检索结果-南通市图书馆

Chinese Chemical Letters 2019年第12期30卷 2009-2012页

作者： Ci Huang Feifei Lu Ke Xu Guanjun Ding Lijun You Jiabin Wang Qiqing Zhang Institute of Biomedical and Pharmaceutical Technology College of Chemistry Fuzhou University

Magnetic polyphosphazene(MPZS) particles coated by Ag nanoparticles(MPZS-Ag) have been developed as surface enhanced Raman spectroscopy(SERS) substrates for sensitive detection of melamine in aqueous solutions and milk samples.5,5’-Dithiobis-(2-nitrobenzoic acid)(DTNB) was used as model analyte to test the SERS activity of the MPZS-Ag particles.The prepared MPZS-Ag particles possess both magnetic responsiveness and excellent SERS properties.SERS detection of different concentrations of melamine aqueous solutions and spiked milk samples were performed by the MPZS-Ag particles.The limit of detection(LOD) of the melamine in aqueous solutions was 10^-7 mol/L(0.0126 mg/L) and 0.6 mg/L in real milk samples using the MPZS-Ag particles as SERS substrates.The LOD of the melamine are much lower than the safety values of Food and Drug Administration and Codex Alimentarius Commission.These results indicate that the MPZS-Ag particles have promising application prospect for SERS analysis in food safety fields.

关键词： Polyphosphazene Magnetic polymer particles Surface enhanced Raman spectroscopy Ag nanoparticles Melamine detection

Excellent Hydrophilic and Anti-bacterial Fouling PVDF Membrane Based on Ag Nanoparticle Self-assembled PCBMA Polymer Brush

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Polymer Science 2017年第7期35卷 809-822页

作者：李建华 De-bin Zhang Xing-xing Ni Hui Zheng 张其清 Institute of Biomedical and Pharmaceutical Technology Fuzhou University Fuzhou 350001 China

A silver nanoparticles-poly（carboxybetaine methacrylate）（AgNPs-PCBMA） nanocomposite was prepared on poly（vinylidene fluoride）（PVDF） membrane surface to improve its hydrophilicity and antifouling properties. Firstly, the PVDF membranes were grafted by PCBMA via physisorbed free radical grafting technique. Then Ag＋ coordinated to the carbonyl group on PCBMA and subsequently was reduced to silver nanoparticles. The hydrophilicity of the PVDF-g- PCBMA/Ag membrane was enhanced with the increasing fixed degree （FD） of AgNPs, and the original water contact angle of membrane was reduced to 33.97°. Additionally, water flux recovery ratio （FRR） and bovine serum albumin （BSA） rejection ratio of PVDF-g-PCBMA/AgNPs membrane were improved from 52% to 93.32% and 28.12% to 91.12%, respectively. Further, the PVDF-g-PCBMA/AgNPs membranes exhibited the more pronounced inhibition zone. The study demonstrated that compared with pure AgNPs or the PCBMA polymer brush, the synergistic effect of PCBMA and AgNPs made PVDF membranes have better hydrophilicity and anti-bacterial performances.

关键词： PVDF membrane Zwitterionic monomer CBMA Silver nanoparticle Antifouling performance Antibacterialability

Physicochemical properties,antimicrobial activity and oil release of fish gelatin films incorporated with cinnamon essential oil

在线全文

学校读者我要写书评

暂无评论

Aquaculture and Fisheries 2017年第4期2卷 185-192页

作者： Jiulin Wu Xinyu Sun Xiaoban Guo Shangying Ge Qiqing Zhang Institute of Biomedical and Pharmaceutical Technology Fuzhou UniversityFuzhou 350002China

Fish skin gelatin films incorporated with various concentrations of cinnamon essential oil(CEO)were prepared and characterized.The results showed that tensile strength(TS),elongation at break(EAB),and water content(WC)of the gelatin based film decreased with the increasing concentrations of CEO,but water vapor permeability(WVP)increased.Addition of CEO improved light barrier property of the film.The Scanning electron microscope(SEM)showed that the heterogeneous surface and porous formation appeared in gelatin-CEO films.Fourier transform infrared spectroscopy analyses(FTIR-ATR)spectra indicated the interactions existed between gelatin and CEO.The gelatin-CEO films exhibited good inhibitory effects against the tested microorganisms(Escherichia coli,Staphylococcus aureus,Aspergillus niger,Rhizopus oryzae,and Paecilomyces varioti)and their antifungal activity seemed to be more effective than the resistance to bacterial growth.In vitro release studies showed an initial burst effect of CEO release and that subsequently slowed down at 40℃,but the initial burst release was not obvious at 4℃.The obtained results suggested that incorporation of CEO as a natural antimicrobial agent into gelatin film has potential for developing as active food packaging.

关键词： Gelatin film Cinnamon essential oil Physical property Antimicrobial activity In vitro release

Vesicular carriers containing phenylethyl resorcinol for topical delivery system; liposomes, transfersomes and invasomes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of pharmaceutical Sciences 2018年第5期13卷 472-484页

作者： Thanaporn Amnuaikit Tunyaluk Limsuwan Pasarat Khongkow Prapaporna Boonme Department of pharmaceutical technology Faculty of Pharmaceutical Sciences Prince of Songkla University institute of biomedical Engineering Faculty of Medicine Prince of Songkla University Nanotec-PSU Center of Excellence on Drug Delivery System

Topical administration of phenylethyl resorcinol(PR) has attracted much attention as skin lightening agent with potent anti-tyrosinase activity. Two novel types of elastic carriers were developed to overcome the limitation of PR as topical delivery by increasing the solubility, stability and decreasing skin irritation compared to conventional liposomes. In addition, it also promotes skin penetration of PR to reach deep skin layer at the target site. The lead formulations were obtained from the invasomes containing 1%(w/v) d-limonene mixed with 10%(v/v) absolute ethanol as the skin enhancer, and transfersomes containing 15%(w/w) sodium deoxycholate(SDC) as edge activator. All formulations gave a vesicle size 50%, and good stability on storage at 30 °C at 75% RH for 4 months. Transfersomes have a lower degree of deformability(6.63%) than invasomes(25.26%). In contrast, the liposomes as rigid vesicles do not show a deformable property. This characteristic affects the skin permeation, and thus, transfersomes with high elastic property provided a significantly higher cumulative amount, steady state flux( J ss) and permeability coefficient( K p) compared to other formulations. However, in vitro PR accumulation in full-thickness newborn pig skin demonstrated that the application of elastic carrier formulations gave significantly higher accumulation than liposomes, and gave anti-tyrosinase activity up to 80%. These results are straightforwardly related to the results of cellular level study. Transfersomes and invasomes showed higher tyrosinase inhibition activity and melanin content reduction when compared to liposomes in B16 melanoma cells. In addition, acute irritation test in rabbits confirmed that these formulations are safe for skin application. Therefore, elastic vesicle carriers have theefficiency to deliver PR into the deep skin in both quantity and effectiveness which are better than conventional liposomes and appropriate for

关键词： Phenylethyl resorcinol Transfersomes Invasomes Tyrosinase inhibition

Development of composite PLGA microspheres containing exenatide-encapsulated lecithin nanoparticles for sustained drug release

在线全文

学校读者我要写书评

暂无评论

Asian Journal of pharmaceutical Sciences 2020年第3期15卷 347-355页

作者： Ni Dong Chune Zhu Junhuang Jiang Di Huang Xing Li Guilan Quan Yang Liu Wen Tan Xin Pan Chuanbin Wu School of pharmaceutical Sciences Sun Yat-sen UniversityGuangzhou 510006China institute of biomedical and pharmaceutical Sciences Guangdong University of TechnologyGuangzhou 510006China Shenyang pharmaceutical University Benxi 117004China College of Pharmacy Chongqing Medical and Pharmaceutical CollegeChongqing 401331China

This study aimed to prepare poly(D, L-lactic-co-glycolic acid) microspheres(PLGA-Ms)by a modified solid-in-oil-in-water(S/O/W) multi-emulsion technique in order to achieve sustained release with reduced initial burst and maintain efficient drug concentration for a prolonged period of time. Composite PLGA microspheres containing exenatideencapsulated lecithin nanoparticles(Ex-NPs-PLGA-Ms) were obtained by initial fabrication of exenatide-loaded lecithin nanoparticles(Ex-NPs) via the alcohol injection method,followed by encapsulation of Ex-NPs into PLGA microspheres. Compared to Ms prepared by the conventional water-in-oil-in-water(W/O/W) technique(Ex-PLGA-Ms), Ex-NPs-PLGAMs showed a more uniform particle size distribution, reduced initial burst release, and sustained release for over 60 d in vitro. Cytotoxicity studies showed that Ms prepared by both techniques had superior biocompatibility without causing any detectable cytotoxicity.In pharmacokinetic studies, the effective drug concentration was maintained for over 30 d following a single subcutaneous injection of two types of Ms formulation in rats, potentially prolonging the therapeutic action of Ex. In addition, administration of Ex-NPs-PLGA-Ms resulted in a more smooth plasma concentration-time profile with a higher area under the curve(AUC) compared to that of Ex-PLGA-Ms. Overall, Ex-NPs-PLGA-Ms prepared by the novel S/O/W method could be a promising sustained drug release system with reduced initial burst release and prolonged therapeutic efficacy.

关键词： Microspheres PLGA Peptides Lipid nanoparticles Sustained drug release

Conjugated Polymers for Combatting Antimicrobial Resistance

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemistry 2022年第6期40卷 759-772页

作者： Zijuan Wang Hao Geng Chenyao Nie Chengfen Xing School of Materials Science and Engineering Hebei University of TechnologyTianjin 300130China Key Laboratory of Biotechnology and pharmaceutical Engineering School of Pharmaceutical SciencesCixi Biomedical Research InstituteWenzhou Medical UniversityWenzhouZhejiang 325035China Key Laboratory of Hebei Province for Molecular Biophysics Institute of BiophysicsHebei University of TechnologyTianjin 300130China

Antimicrobial resistance(AMR)is one of the major challenges in pathogenic infections,which endangers human health and brings devastating public health threats.Therefore,developing new materials to reduce the over-reliance on antibiotics or to replace antibiotics is in great need to solve this problem.Conjugated polymers(CPs)with excellent light-harvesting properties and good biocompatibility are ideal candidate materials for combat-ting AMR through photodynamic antimicrobial therapy(PACT)by sensitizing oxygen and photothermal therapy(PTT)by light to heat conversion.Sig-nificant works of CPs-based antimicrobial approaches have been reported in recent years.Herein,we summarize the types of CPs-based antimicrobial materials,illustrate their antimicrobial mechanisms,and discuss their applications in combating AMR mainly by PACT or antimicrobial PTT.In addi-tion,we proposed some other antimicrobial treatment strategies based on CPs,hoping to provide inspiring insight for overcoming AMR.

关键词： Polymers Antimicrobial resistance Photochemistry Materials science Phototherapy

Preparation of solid pharmaceutical forms of celecoxib with immediate-release and sustained-release by using wet granulation techniques and evaluation of their in vitro characteristics

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Chinese pharmaceutical Sciences 2022年第7期31卷 536-544页

作者： Ahmed Alkhodri Svetlana Suslina Department of General pharmaceutical and biomedical technology Medical InstitutePeoples’Friendship University of RussiaStreet Miklukho-Maklaya 6Moscow 117198Russia

In the present study,we aimed to formulate matrix tablets of celecoxib with immediate-release(IR)and sustained-release(SR)and evaluate the influence of different types and concentrations of polymers on drug release characteristics in vitro.All formulations were prepared by using the wet granulation technique.One formulation(F5)with the traditional release was composed without release-control polymers.In contrast,four formulations(F1–F4)with prolonged action were designed by using different cellulosic polymers,such as hydroxypropyl methylcellulose polymers(HPMC-K100M and HPMC-Е6),hydroxypropyl cellulose with high viscosity grade(HPCh),carboxymethyl cellulose(CMC),and ethylcellulose(EC-10 cps).All tablet formulations contained sodium lauryl sulfate(SLS),polyvinylpyrrolidone(PVP-k30),microcrystalline cellulose(MCC),and lactose monohydrate.The MCC and PVP-k30 were used in a fixed quantity in all formulations except for formulation F5,which contained 10%and 2.7%of them,respectively.The effects of polymer viscosity grade and its quantity on celecoxib release from two formulations containing the same polymer were studied.Drug release in vitro was evaluated in phosphate buffer(pH=7.4).The amount of celecoxib released in phosphate medium was calculated by preparing a standard series.In vitro profile indicated that formulations F1(HPMC-K100M)and F2(HPMC-E6)extended the drug release for 18 h(82%)and 10 h(96.001%),respectively.Formulations F3and F4 released 72.09%and 59.8%of their contents during 18 h,respectively,and thus their effects could last for more than a day.However,IR formulation(F5)released more than 90%of its content within 1–2 h.MS Excel was used to analyze the dissolution profile data for drug release kinetics,such as first-order,Zero-order,Higuchi,and Korsmeyer-Peppas models.The release mechanism of all formulations was CaseⅡrelaxation release.This study showed that cellulosic derivative polymers could successfully prolong IR of matrix tablet formulation.

关键词： Immediate release Sustained release Celecoxib HPMC Wet granulation Dissolution profile

Native proteomics utilizing nondenaturing gel electrophoresis and quantitative MS mapping

在线全文

学校读者我要写书评

暂无评论

Native proteomics utilizing nondenaturing gel electrophoresi...

Engineered gold/black phosphorus nanoplatforms with remodeling tumor microenvironment for sonoactivated catalytic tumor theranostics

2018年中国质谱学术大会（CMSC2018）

作者： Ya Jin institute of biomedical and pharmaceutical Sciences Guangdong University of Technology

Gel electrophoresis is one of the most widely technique in life science. Nondenaturing 1 D and 2 D PAGE provide high-resolution separation of proteins under conditions that their native structures, interactions and enzymatic activities could be largely retained. These embedded information, however, though highly valuable for structural and functional studies, is often difficult to be unveiled. In our works, a series of methods based on a strategy of native MS mapping were developed for large-scale analysis of proteins and protein-protein interactions. The methods generally include the following steps, i) a proteomic sample is separated by nondenaturing 1 DE/2 DE, ii) an area of interest or the whole area of the gel is grid-cut into equal squares, iii) all the gel pieces are analyzed in standard procedures of in-gel digestion followed by quantitative LC-MS/MS, iv) a quantity distribution of each MS-detected protein in the gel(area) is reconstructed(a native map), and v) pattern comparison of the native maps and bioinformatic analysis is conducted to obtain information on subunit structures and protein interactions. This strategy has been applied for analyzing interactions of human high-density lipoprotein(HDL) and low-density lipoprotein(LDL), respectively. By mapping all proteins in the areas of the two lipoproteins on nondenaturing 2 D gels of plasma, followed with map comparison with the major component apolipoproteins, 18 HDL and 22 LDL apoliproteins were revealed [1,2]. The strategy was then extensively examined for analysis of cellular proteins. Soluble proteins of human bronchial smooth muscle cells were separated by nondenaturing 2 DE and the gel was grid-cut into 972 squares. Native 2 D maps of totally 4323 proteins were reconstructed. To evaluate the degree of map similarity, an algorithm and corresponding computer programs were developed to calculate an "overlap factor" of each protein map against each of the other maps. Maps of 2328 proteins that were detected in 3 or more squares were evaluated and 43

关键词： native proteomics MS mapping protein interactions nondenaturing 1DE/2DE LC-MS/MS

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Bioactive Materials 2022年第4期7卷 515-525页

作者： Ting Chen Weiwei Zeng Changjun Tie Mian Yu Huisong Hao Yang Deng Qianqian Li Hairong Zheng Meiying Wu Lin Mei School of pharmaceutical Sciences(Shenzhen) Sun Yat-sen UniversityShenzhen518107China Paul C.Lauterbur Research Center for biomedical Imaging Institute of Biomedical and Health EngineeringShenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen518055China Tianjin Key Laboratory of biomedical Materials Key Laboratory of Biomaterials and Nanotechnology for Cancer ImmunotherapyInstitute of Biomedical EngineeringChinese Academy of Medical SciencesPeking Union Medical CollegeTianjin300192China

The imbalance between oxidants and antioxidants in cancer cells would evoke oxidative stress-induced cell death,which has been demonstrated to be highly effective in treating malignant tumors.Sonodynamic therapy(SDT)adopts ultrasound(US)as the excitation source to induce the production of reactive oxygen species(ROS),which emerges as a noninvasive therapeutic strategy with deep tissue penetration depth and high clinical safety.Herein,we construct novel sonoactivated oxidative stress amplification nanoplatforms by coating MnO_(2) on Au nanoparticle-anchored black phosphorus nanosheets and decorating soybean phospholipid subsequently(Au/BP@MS).The Au/BP@MS exhibit increased ROS generation efficiency under US irradiation in tumor tissues due to Au/BP nanosensitizer-induced improvement of electron-hole separation as well as MnO_(2)-mediated O_(2) generation and GSH depletion,thus leading to notable inhibition effect on tumor growth.Moreover,tumor microenvironment-responsive biodegradability of Au/BP@MS endows them with enhanced magnetic resonance imaging guidance and clinical potential for cancer theranostics.

关键词： Black phosphorus Oxidative stress Tumor microenvironment Sonodynamic therapy Theranostic

Local dose-dense chemotherapy for triple-negative breast cancer via minimally invasive implantation of 3D printed devices

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of pharmaceutical Sciences 2024年第1期19卷 69-85页

作者： Noehyun Myung Hyun-Wook Kang Department of biomedical Engineering Ulsan National Institute of Science and Technology(UNIST)Ulju-gun 44919South Korea

Dose-dense chemotherapy is the preferred first-line therapy for triple-negative breast cancer(TNBC),a highly aggressive disease with a poor prognosis.This treatment uses the same drug doses as conventional chemotherapy but with shorter dosing intervals,allowing for promising clinical outcomes with intensive treatment.However,the frequent systemic administration used for this treatment results in systemic toxicity and low patient compliance,limiting therapeutic efficacy and clinical benefit.Here,we report local dose-dense chemotherapy to treat TNBC by implanting 3D printed devices with timeprogrammed pulsatile release profiles.The implantable device can control the time between drug releases based on its internal microstructure design,which can be used to control dose density.The device is made of biodegradable materials for clinical convenience and designed for minimally invasive implantation via a trocar.Dose density variation of local chemotherapy using programmable release enhances anti-cancer effects in vitro and in vivo.Under the same dose density conditions,device-based chemotherapy shows a higher anticancer effect and less toxic response than intratumoral injection.We demonstrate local chemotherapy utilizing the implantable device that simulates the drug dose,number of releases,and treatment duration of the dose-dense AC(doxorubicin and cyclophosphamide)regimen preferred for TNBC treatment.Dose density modulation inhibits tumor growth,metastasis,and the expression of drug resistance-related proteins,including p-glycoprotein and breast cancer resistance protein.To the best of our knowledge,local dose-dense chemotherapy has not been reported,and our strategy can be expected to be utilized as a novel alternative to conventional therapies and improve anti-cancer efficiency.

关键词： Dose-dense chemotherapy Triple-negative breast cancer 3D printing Pulsatile release Local drug delivery systems