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BFP1:One of 700 Arabidopsis F-box proteins mediates degradation of JA oxidases to promote plant immunity

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Molecular Plant 2024年第3期17卷 375-376页

作者： Claus Wasternack Bettina Hause Department of Molecular Signal Processing Leibniz Institute of Plant Biochemistry7 Weinberg 306120 Halle(Saale)Germany Department of Cell and Metabolic Biology Leibniz Institute of Plant Biochemistry06120 Halle(Saale)Germany

A switch off in jasmonate(JA)signaling by hydroxylation upon wounding was described in 2008(Miersch et al.,2008).This initiated the cloning of CYP450 enzymes involved in hydroxylation and carboxylation of JA and its amino acid conjugate JA-le,the most active form of JA compounds(reviewed by Wasternack and Feussner[2018]and Heitz et al.[2019]).Due to the central role of JA and JA-lle in plant stress responses and development(Wasternack and Hause,2013),catabolism of JA and its derivatives is an essential component in the sustainment of homeostasis of active JA compounds(Howe et al.,2018;Heitz et al.,2019).Catabolism of JA and JAIle takes place by JA-induced oxygenases(JOXs),also named JA-oxidases(JAOs),and by hydroxylation(CYP94B3/B1)followed by carboxylation(CYP94C1),respectively(Heitz et al.,2019).Another reaction performed by IAR3/ILL6 results in the deconjugation of JA-lle and 12-OH-JA-le to JA and 12-OH-JA,respectively(Heitz et al.,2019;Wasternack and Feussner,2018).

关键词： conjugate initiated media

THE NUCLEOTIDE SEQUENCE OF SURFACE ANTIGEN GENE OF HEPATITIS B VIRUS SUBTYPE adr

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中国科学B辑(英文版) 1984年第9期27卷 926-935页

作者：甘人宝沈绿萍储美瑾李载平 Shanghai institute of biochemistry Academia Sinica Academia Sinica

The nucleotide sequence of the XhoI-BamHI fragment (1279bp), which contains the sur-face antigen gene (S gene) of HBVadr, was determined by Maxam and Gilbert’s method.By comparing the differences both of the nuclectide sequence in the S gene and its codedamino acid sequence between adr and those reported for adw, ayw and adyw, some new var-iation sites were discovered. The differences were mainly distributed in the two hydrophilieregions. However, at those sites which might show biological function, there were no varia-tions among different subtypes, they are relatively conservative in heredity and evolution.Comparing the variation of the nucleotide sequence in the S gene region with that in thenon-S gene region, it is shown that the frequency of variation in the non-S gene regiondoubled that in the S gene region. Tim S gene region is more conservative.

关键词： conservative SURFACE Gilbert BamHI varia 司卜 nanometer advice genomic labeled

EXPRESSION OF SURFACE ANTIGEN GENE OF HUMAN HEPATITIS B VIRUS SEROTYPE adr IN Escherichia coli

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中国科学B辑(英文版) 1985年第6期28卷 618-625页

作者：敖世洲周翊钟潘铁城李载平 Shanghai institute of biochemistry Academia Sinica Academia Sinica

The construction of an expression plasmid of hepatitis B virus surface antigen (HBsAg) gone from the cloned hepatitis B virus (HBV) genome subtype adr is reported. The expression products of this plasmid in E. coli were detected by means of radioimmunoassay in competitive suppression and polyacrylamide-SDS gel electrophoresis. The presence of a fusion protein containing HBsAg was confirmed.

关键词： plasmid cloned SURFACE suppression competitive Agarose linker polyacrylamide agarose HBsAb

Unlocking new potential of clinical diagnosis with artificial intelligence:Finding new patterns of clinical and lab data

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World Journal of Diabetes 2024年第3期15卷 308-310页

作者： Pradeep Kumar Dabla Department of biochemistry Govind Ballabh Pant Institute of Postgraduate Medical Education and ResearchDelhi 110002India

Recent advancements in science and technology,coupled with the proliferation of data,have also urged laboratory medicine to integrate with the era of artificial intelligence(AI)and machine learning(ML).In the current practices of evidencebased medicine,the laboratory tests analysing disease patterns through the association rule mining(ARM)have emerged as a modern tool for the risk assessment and the disease stratification,with the potential to reduce cardiovascular disease(CVD)mortality.CVDs are the well recognised leading global cause of mortality with the higher fatality rates in the Indian population due to associated factors like hypertension,diabetes,and lifestyle choices.AI-driven algorithms have offered deep insights in this field while addressing various challenges such as healthcare systems grappling with the physician shortages.Personalized medicine,well driven by the big data necessitates the integration of ML techniques and high-quality electronic health records to direct the meaningful outcome.These technological advancements enhance the computational analyses for both research and clinical practice.ARM plays a pivotal role by uncovering meaningful relationships within databases,aiding in patient survival prediction and risk factor identification.AI potential in laboratory medicine is vast and it must be cautiously integrated while considering potential ethical,legal,and privacy concerns.Thus,an AI ethics framework is essential to guide its responsible use.Aligning AI algorithms with existing lab practices,promoting education among healthcare professionals,and fostering careful integration into clinical settings are imperative for harnessing the benefits of this transformative technology.

关键词： Laboratory medicine Artificial intelligence Machine learning Association rule mining Cardiovascular diseases

THE POLYHEDRIN GENE OF Bombyx mori NUCLEAR POLYHEDROSIS VIRUS

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Science in China,Ser.B 1985年第10期28卷 1051-1059页

作者：王珩忻纪厚顾懋治李载平 Shanghai institute of biochemistry Academia Sinica

Cloned SalI fragments of Bombyx mori nuclear polyhedrosis virus (BmSNPV) DNA were screened with the polyhedrin gene of Autographa californica nuclear polyhedrosis virus as a probe. One positive clone, pBN61, with an insert of 1.65 Kb, was obtained. The Hind-Ⅲ, HpaⅡ and AluⅠ maps of the insert were constructed. Part of its nucleotide sequence has been determined. The 46 amino acid sequence, as determined from the nucleotide sequence, was compared with the reported sequence of BmSNPV polyhedrin. Only ono amino acid difference has been found. It is likely that clone pBN61 contains the whole BmSNPV polyhedrin gene.

关键词： NPV THE POLYHEDRIN GENE OF Bombyx mori NUCLEAR POLYHEDROSIS VIRUS DNA gene

Expression, Purification and Sublocalization of SARS-CoV Nucleocapsid Protein in Insect Cells

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Acta Biochimica et Biophysica Sinica 2004年第11期36卷 754-758页

作者： Ai-Xia REN, You-Hua XIE, Yu-Ying KONG, Guan-Zhen YANG, Yao-Zhou ZHANG,Yuan WANG, and Xiang-Fu WUinstitute of biochemistry and Biotechnology, College of Life Science, Zhejiang University, Hangzhou 310029, China State Key Laboratory of Molecular Biology, Shanghai institute of biochemistry and cell Biology, Shanghai institutes for Biological Sciences,Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China institute of biochemistry, Zhejiang University of Sciences, Hangzhou 310018, China institute of biochemistry and Biotechnology College of Life Science Zhejiang University Hangzhou 310029 China.

The causative agent of severe acute respiratory syndrome (SARS) is a previously unidentified coronavirus, SARS-CoV. The nucleocapsid (N) protein of SARS-CoV is a major viral protein recognized by acute and early convalescent sera from SARS patients. To facilitate the studies on the function and structure of the N protein, this report describe the expression and purification of recombinant SARS-CoV N protein using the baculovirus

关键词： severe acute respiratory syndrome (SARS) SARS coronavirus nucleocapsid protein baculovirus insect cells

CODING ASSIGNMENTS OF EACH mRNA SYNTHESIZED IN VITRO AND PROTEIN COMPONENTS OF CPV OF SILKWORM BOMBYX MORI

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Science in China,Ser.B 1983年第2期26卷 162-166页

作者：巫爱珍戴仁鸣沈学仁孙玉昆 Shanghai institute of biochemistry Academia Sinica

The coding propertics of each mRNA synthesized in vitro of CPV have been examined. The mRNAs were separated by polyacrylamide gel electrophoresis and isolated from the gel slices. The individual mRNA was then translated into the wheat germ cell-free protein synthesizing system. The products of translation were analyzed by the immuno-reaction with the 125I-IgG of each of the five electrophoretic protein components of CPV. The coding assignments were deduced on the basis of the immuuo-reaction as follows: mRNA1, mRNA3 and mRNA9 encode group P1 of viral proteins, mRNA2 and mRNA6 encode group P2 and P3, respectively, mRNA7 and mRNA6 encode group P4 while mRNA9 encode group P5.There axe more than 10 polypeptides in the CPV virion as demonstrated by two-dimensional electrophoresis.

关键词： mRNA CODING ASSIGNMENTS OF EACH mRNA SYNTHESIZED IN VITRO AND PROTEIN COMPONENTS OF CPV OF SILKWORM BOMBYX MORI CPV

Bone marrow microRNA-34a is a good indicator for response to treatment in acute myeloid leukemia

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Oncology Research 2024年第3期32卷 577-584页

作者： MONA SA BDELLATEIF NAGLAA MHASSAN MAHMOUD MKAMEL YOMNA MEL-MELIGUI Department of Cancer Biology Medical Biochemistry and Molecular BiologyNational Cancer InstituteCairo UniversityCairo11976Egypt Department of Cinical Pathology National Cancer InstituteCairo UniversityCairo11976Egypt

Background:microRNA 34a(miR 34a)had been reported to have a diagnostic role in acute myeloid leukemia(AML).However,its value in the bone marrow(BM)of AML patients,in addition to its role in response to therapy is still unclear.The current study was designed to assess the diagnostic,prognostic,and predictive significance of miR 34a in the BM of AML patients.Methods:The miR.34a was assed in BM aspirate of 82 AML patients in relation to 12 normal control subjects using qRT-PCR.The data were assessed for correlation with the relevant dinical critenia,response to therapy,disease-free survival(DFS),and overall survival(OS)rates.Results:miR.34a was significantly downregulated in AML patients[0.005(3.3×10^(-6)-1.32)],compared to the control subjects[0.108(3.2× 10^(-4)-1.64),p=0.021].The.median relative quantification(RQ)of miR-34a was 0.106(range;0-32.12).The specifaity,sensitivity,and area under the curve(AUC)for the diagnosis of AML were(58.3%,69.5%,0.707,respectively,p=0.021).patients with upregulated miR-34a showed decreased platelets count<34.5 × 10^(9)/L,and achieved early complete remission(CR,p=0.031,p=0.044,respectively).Similarly,patients who were refractory to therapy showed decreased miR 34a levels in comparison to those who achieved CR[0.002(0-0.01)and 0.12(0-32.12),respectively,p=0.002].Therefore,miR 34a could significantly identify patients with CR with a specificity of 75%and sensitivity of 100%at a cut-off of 0.014(AUC=0.927,p=0.005).There was no considerable association between miR-34a expression and survival rates of the induded AML patients.Condusion:miR-34a could be a beneficial diagnostic biomarker for AML patients.In addition,it serves as a good indicator for response to therapy,which could possibly identify patients who are refractory to treatment with 100%sensitivity and 75%specificity.

关键词： AML miR 34a microRNA Leukemia

CLONING AND RESTRICTION MAPPING OF HUMAN HBV GENOME SEROTYPE adr

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Science in China,Ser.B 1983年第9期26卷 954-960页

作者：吴祥甫周翊钟冯宗铭李载平夏绍源 Shanghai institute of biochemistry Academia Sinica Hygienic and Anti-epidemic Station of Shanghai

A Bam HI cleaved 3.2 Kb fragment from the HBV adr genome was cloned in E. coli, using pBR322 as vector. sixteen restriction sites from the action of Bam HI, Hind Ⅲ, Bgl Ⅰ, Bgl Ⅱ, Ava Ⅰ, Hinc Ⅱ, Sph Ⅰ, Xba Ⅰ, Xho Ⅰ and Sst Ⅱ were determined and mapped. No cleavage sites were found for Eco RI, Pst Ⅰ, Sma Ⅰ, Hpa Ⅰ, Kpn Ⅰ, Pvu Ⅱ and Sst Ⅰ.The restriction map for HBV adr is significantly different from those reported for the subtypes adw, ayw and adyw.

关键词： HBV CLONING AND RESTRICTION MAPPING OF HUMAN HBV GENOME SEROTYPE adr DNA