检索结果-南通市图书馆

Morphological and Hormonal Identifi cation of Porcine Atretic Follicles and Relationship Analysis of Hormone Receptor Levels During Granulosa Cell Apoptosis In vivo

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2014年第5期13卷 1058-1064页

作者： YU De-bing YU Min-li LIN Fei JIANG Bao-chun YANG Li-na WANG Si-yu ZHAO Ying WNAG Zheng-chao Department of animal genetics Breeding and Reproduction College of Animal Science and Technology Nanjing Agricultural University

Recent reports have demonstrated that follicular atresia is initiated or caused by granulosa cell apoptosis followed by theca cell degeneration in mammalian ovaries, but the mechanism of follicular atresia is still to be elucidated. Therefore, our present study was designed to examine our hypothesis that the changes of follicular microenvironment induce the granulosa cell apoptosis during pocrine follicular atresia in vivo. We firstly isolated intact porcine antral follicles and identified them into three groups, healthy follicles （HF）, early atretic follicles （EAF） and progressed atretic follicles （PAF） through morphology and histology. To further confirm their status, we detected hormone levels in follicular fluids and the expression level of apoptosis gene Bax in granulosa cells. The rate of progesterone （P） and estradiol （E2） was increased with the expression of Bax, indicating hormone can be used as a marker of granulosa cell apoptosis or follicular atresia. Finally, we analyzed the expression level of hormone receptor genes in granulosa cells and their relationship with follicular atresia. In PAF, the expression of Progesterone receptor （PGR） was increased significantly while estradiol receptor （ER） had no notable changes, which suggesting the increased-PGR accelerated the effect of P-stimulated granulosa cell apoptosis. The dramatic increasing of androgen receptor （AR） expression in PAF and the obvious increase of tumor necrosis factor-u receptor （TNFR） in EAF indicated that there are different pathways regulating granulosa cell apoptosis during follicular atresia. Together, our results suggested that different pathways of granulosa cell apoptosis was induced by changing the follicular microenvironment during follicular atresia.

关键词： granulosa cell apoptosis follicular atresia progesterone estradiol porcine

Estimation of inbreeding and identification of regions under heavy selection based on runs of homozygosity in a Large White pig population

在线全文

学校读者我要写书评

暂无评论

Journal of animal Science and Biotechnology 2020年第4期11卷 965-974页

作者： Liangyu Shi Ligang Wang Jiaxin Liu Tianyu Deng Hua Yan Longchao Zhang Xin Liu Hongmei Gao Xinhua Hou Lixian Wang Fuping Zhao Key Laboratory of animal genetics Breeding and Reproduction(poultry)of Ministry of AgricutureInstitute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193China

Background: Runs of homozygosity(ROHs) are homozygous segments of the genome where the two haplotypes inherited from the parents are identical. The current availability of genotypes for a very large number of single nucleotide polymorphisms(SNPs) is leading to more accurate characterization of ROHs in the whole genome. Here,we investigated the occurrence and distribution of ROHs in 3,692 Large White pigs and compared estimates of inbreeding coefficients calculated based on ROHs(FROH), homozygosity(FHOM), genomic relationship matrix(FGRM)and pedigree(FPED). Furthermore, we identified genomic regions with high ROH frequencies and annotated their candidate ***: In total, 176,182 ROHs were identified from 3,569 animals, and all individuals displayed at least one ROH longer than 1 Mb. The ROHs identified were unevenly distributed on the autosomes. The highest and lowest coverages of Sus scrofa chromosomes(SSC) by ROH were on SSC14 and SSC13, respectively. The highest pairwise correlation among the different inbreeding coefficient estimates was 0.95 between FROH_totaland FHOM, while the lowest was-0.083 between FGRMand FPED. The correlations between FPEDand FROHusing four classes of ROH lengths ranged from 0.18 to 0.37 and increased with increasing ROH length, except for ROH > 10 Mb. Twelve ROH islands were located on four chromosomes(SSC1, 4, 6 and 14). These ROH islands harboured genes associated with reproduction, muscular development, fat deposition and adaptation, such as SIRT1, MYPN, SETDB1 and ***: FROHcan be used to accurately assess individual inbreeding levels compared to other inbreeding coefficient estimators. In the absence of pedigree records, FROHcan provide an alternative to inbreeding *** findings can be used not only to effectively increase the response to selection by appropriately managing the rate of inbreeding and minimizing the negative effects of inbreeding depression but also to help detect genomic regions with an

关键词： Candidate genes Inbreeding coefficients Runs of homozygosity Sus scrofa

Genome-wide identification of RNA editing in seven porcine tissues by matched DNA and RNA high-throughput sequencing

在线全文

学校读者我要写书评

暂无评论

Journal of animal Science and Biotechnology 2019年第2期10卷 339-352页

作者： Yuebo Zhang Longchao Zhang Jingwei Yue Xia Wei Ligang Wang Xin Liu Hongmei Gao Xinhua Hou Fuping Zhao Hua Yan Lixian Wang Key Laboratory of animal(Poultry)genetics Breeding and reproduction Ministry of Agriculture institute of animal Science Chinese Academy of Agricultural SciencesBeijing 100193China

Background: RNA editing is a co/posttranscriptional modification mechanism that increases the diversity of transcripts, with potential functional consequences. The advent of next-generation sequencing technologies has enabled the identification of RNA edits at unprecedented throughput and resolution. However, our knowledge of RNA editing in swine is still ***: Here, we utilized RES-Scanner to identify RNA editing sites in the brain, subcutaneous fat, heart, liver,muscle, lung and ovary in three 180-day-old Large White gilts based on matched strand-specific RNA sequencing and whole-genome resequencing datasets. In total, we identified 74863 editing sites, and 92.1% of these sites caused adenosine-to-guanosine(A-to-G) conversion. Most A-to-G sites were located in noncoding regions and generally had low editing levels. In total, 151 A-to-G sites were detected in coding regions(CDS), including 94 sites that could lead to nonsynonymous amino acid changes. We provide further evidence supporting a previous observation that pig transcriptomes are highly editable at PRE-1 elements. The number of A-to-G editing sites ranged from 4155(muscle) to 25001(brain) across the seven tissues. The expression levels of the ADAR enzymes could explain some but not all of this variation across tissues. The functional analysis of the genes with tissuespecific editing sites in each tissue revealed that RNA editing might play important roles in tissue ***, more pathways showed significant enrichment in the fat and liver than in other tissues, while no pathway was enriched in the ***: This study identified a total of 74863 nonredundant RNA editing sites in seven tissues and revealed the potential importance of RNA editing in tissue function. Our findings largely extend the porcine editome and enhance our understanding of RNA editing in swine.

关键词： ADAR A-to-G High-throughput sequencing RNA editing Swine

The landscape of chromatin accessibility in skeletal muscle during embryonic development in pigs

在线全文

学校读者我要写书评

暂无评论

Journal of animal Science and Biotechnology 2021年第4期12卷 1281-1295页

作者： Jingwei Yue Xinhua Hou Xin Liu Ligang Wang Hongmei Gao Fuping Zhao Lijun Shi Liangyu Shi Hua Yan Tianyu Deng Jianfei Gong Lixian Wang Longchao Zhang Key Laboratory of animal(Poultry)genetics Breeding and reproduction Ministry of AgricultureInstitute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100193China

Background:The development of skeletal muscle in pigs during the embryonic stage is precisely regulated by transcriptional mechanisms,which depend on chromatin ***,how chromatin accessibility plays a regulatory role during embryonic skeletal muscle development in pigs has not been *** gain insight into the landscape of chromatin accessibility and the associated genome-wide transcriptome during embryonic muscle development,we performed ATAC-seq and RNA-seq analyses of skeletal muscle from pig embryos at 45,70 and 100 days post coitus(dpc).Results:In total,21,638,35,447 and 60,181 unique regions(or peaks)were found across the embryos at 45 dpc(LW45),70 dpc(LW70)and 100 dpc(LW100),*** than 91%of the peaks were annotated within−1 kb to 100 bp of transcription start sites(TSSs).First,widespread increases in specific accessible chromatin regions(ACRs)from embryos at 45 to 100 dpc suggested that the regulatory mechanisms became increasingly complicated during embryonic ***,the findings from integrated ATAC-seq and RNA-seq analyses showed that not only the numbers but also the intensities of ACRs could control the expression of associated ***,the motif screening of stage-specific ACRs revealed some transcription factors that regulate muscle developmentrelated genes,such as MyoG,Mef2c,and *** potential transcriptional repressors,including E2F6,OTX2 and CTCF,were identified among the genes that exhibited different regulation trends between the ATAC-seq and RNA-seq ***:This work indicates that chromatin accessibility plays an important regulatory role in the embryonic muscle development of pigs and regulates the temporal and spatial expression patterns of key genes in muscle development by influencing the binding of transcription *** results contribute to a better understanding of the regulatory dynamics of genes involved in pig embryonic skeletal muscle development.

关键词： Chromatin accessibility Embryo Pig Skeletal muscle Transcriptome

Dominant changes in the breast muscle lipid profiles of broiler chickens with wooden breast syndrome revealed by lipidomics analyses

在线全文

学校读者我要写书评

暂无评论

Journal of animal Science and Biotechnology 2022年第6期13卷 1703-1713页

作者： Ranran Liu Fuli Kong Siyuan Xing Zhengxiao He Lu Bai Jiahong Sun Xiaodong Tan Di Zhao Guiping Zhao Jie Wen institute of animal Sciences Chinese Academy of Agricultural SciencesState Key Laboratory of Animal NutritionKey Laboratory of Animal(Poultry)Genetics Breeding and ReproductionMinistry of AgricultureBeijingChina

Background: Chicken is the most consumed meat worldwide and the industry has been facing challenging myopathies. Wooden breast(WB), which is often accompanied by white striping(WS), is a serious myopathy adversely affecting meat quality of breast muscles. The underlying lipid metabolic mechanism of WB affected broilers is not fully ***: A total of 150 chickens of a white-feathered, fast-growing pure line were raised and used for the selection of WB, WB + WS and control chickens. The lipids of the breast muscle, liver, and serum from different chickens were extracted and measured using ultra performance liquid chromatography(UPLC) plus Q-Exactive Orbitrap tandem mass spectrometry. In the breast, 560 lipid molecules were identified. Compared to controls, 225/225 of 560 lipid molecules(40.2%) were identified with differential abundance(DA), including 92/100 significantly increased neutral lipids and 107/98 decreased phospholipids in the WB/WB + WS groups, respectively. The content of monounsaturated fatty acids(MUFA) was significantly higher, and the polyunsaturated fatty acids(PUFA) and saturated fatty acids(SFA) were significantly lower in the affected breasts. In the liver, 434 lipid molecules were identified, and 39/61 DA lipid molecules(6.7%/14.1%) were detected in the WB and WB + WS groups, respectively. In the serum, a total of 529 lipid molecules were identified and 4/44 DA lipid molecules(0.8%/8.3%) were detected in WB and WB + WS group, respectively. Compared to controls, the content of MUFAs in the serum and breast of the WB + WS group were both significantly increased, and the content of SFAs in two tissues were both significantly decreased. Only five lipid molecules were consistently increased in both liver and serum in WB + WS ***: We have found for the first time that the dominant lipid profile alterations occurred in the affected breast muscle. The relative abundance of 40.2% of lipid molecules were changed and is characteri

关键词： Broiler chicken Lipid profle Liver Serum Wooden breast

More abundant and healthier meat:will the MSTN editing epitome empower the commercialization of gene editing in livestock?

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2022年第2期65卷 448-450页

作者： Xiaolong Wang Björn Petersen Key Laboratory of animal genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling 712110China institute of Farm animal genetics Friedrich-Loeffler-Institute(FLI)Mariensee 31535Germany

In recent years,various gene-edited crops and livestock have been developed to find rapid and unconventional solutions to boost agriculture,animal breeding,environmental sustain-ability,and animal *** the animal production field,significant gene-edited livestock with economic beneficial traits,like the MSTN-edited double muscles,can be com-mercialized on a large scale to benefit more people with real *** demand for animal proteins increases an-nually with improvement of living conditions in modern societies and developing ***,the number of people with special needs for healthier meat(with high-protein and low-fat)is growing(e.g.,elderly patients with cardio/cerebrovascular diseases).With steady global en-vironmental deterioration,increasing human population,growing food demand,continuous disease outbreaks,and concerns regarding animal welfare。

关键词： abundant crops breeding

同方期刊数据库

Sirtuin 3 regulation:a target to alleviateβ-hydroxybutyric acid-induced mitochondrial dysfunction in bovine granulosa cells

在线全文

学校读者我要写书评

暂无评论

Journal of animal Science and Biotechnology 2023年第4期14卷 1377-1394页

作者： Shanjiang Zhao Jianfei Gong Yi Wang Nuo Heng Huan Wang Zhihui Hu Haoyu Wang Haobo Zhang Huabin Zhu State Key Laboratory of animal Nutrition Key Laboratory of Animal GeneticsBreeding and Reproduction of Ministry of Agriculture and Rural AffairsInstitute of Animal ScienceChinese Academy of Agricultural SciencesBeijingChina

Background During the transition period,the insufficient dry matter intake and a sharply increased in energy consumption to produce large quantities of milk,high yielding cows would enter a negative energy balance(NEB)that causes an increase in ketone bodies(KBs)and decrease in reproduction *** excess concentrations of circulating KBs,represented byβ-hydroxybutyric acid(BHBA),could lead to oxidative damage,which potentially cause injury to follicular granulosa cells(fGCs)and delayed follicular *** 3(Sirt3)regulates mitochondria reactive oxygen species(mitoROS)homeostasis in a beneficial manner;however,the molecular mechanisms underlying its involvement in the BHBA-induced injury of fGCs is poorly *** aim of this study was to explore the protection effects and underlying mechanisms of Sirt3 against BHBA overload-induced damage of *** Our findings demonstrated that 2.4 mmol/L of BHBA stress increased the levels of mitoROS in bovine *** investigations identified the subsequent mitochondrial dysfunction,including an increased abnormal rate of mitochondrial architecture,mitochondrial permeability transition pore(MPTP)opening,reductions in mitochondrial membrane potential(MMP)and Ca^(2+)release;these dysfunctions then triggered the caspase cascade reaction of apoptosis in ***,the overexpression of Sirt3 prior to treatment enhanced mitochondrial autophagy by increasing the expression levels of Beclin-1,thus preventing BHBA-induced mitochondrial oxidative stress and mitochondrial dysfunction in ***,our data suggested that the AMPK-mTOR-Beclin-1 pathway may be involved in the protective mechanism of Sirt3 against cellular injury triggered by BHBA *** These findings indicate that Sirt3 protects fGCs from BHBA-triggered injury by enhancing autophagy,attenuating oxidative stress and mitochondrial *** study provides new strategies to mitigate the fGCs injury caused by excess

关键词： BHBA Dairy cows Granulosa cells Ketosis Mitochondrial function Sirt3

Building a sequence map of the pig pan-genome from multiple de novo assemblies and Hi-C data

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2020年第5期63卷 750-763页

作者： Xiaomeng Tian Ran Li Weiwei Fu Yan Li Xihong Wang Ming Li Duo Du Qianzi Tang Yudong Cai Yiming Long Yue Zhao Mingzhou Li Yu Jiang Key Laboratory of animal genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100China institute of animal genetics and Breeding College of Animal Science and TechnologySichuan Agricultural UniversityChengdu 611130China

Pigs were domesticated independently in the Near East and China,indicating that a single reference genome from one individual is unable to represent the full spectrum of divergent sequences in pigs ***,12 de novo pig assemblies from Eurasia were compared in this study to identify the missing sequences from the reference *** a result,72.5 Mb of nonredundant sequences(~3% of the genome)were found to be absent from the reference genome(Sscrofa11.1)and were defined as *** the pan-sequences,9.0 Mb were dominant in Chinese pigs,in contrast with their low frequency in European *** sequence dominant in Chinese pigs contained the complete genic region of the tazarotene-induced gene 3(TIG3)gene which is involved in fatty acid *** flanking sequences and Hi-C based methods,27.7% of the sequences could be anchored to the reference *** supplementation of these sequences could contribute to the accurate interpretation of the 3D chromatin structure.A web-based pan-genome database was further provided to serve as a primary resource for exploration of genetic diversity and promote pig breeding and biomedical research.

关键词： pan-genome pig reference genome 3D chromatin structure presence-absence variation

Meclofenamic acid represses spermatogonial proliferation through modulating m^6A RNA modification

在线全文

学校读者我要写书评

暂无评论

Journal of animal Science and Biotechnology 2019年第4期10卷 919-930页

作者： Tao Huang Jiayin Guo Yinghua Lv Yi Zheng Tongying Feng Qiang Gao Wenxian Zeng Key Laboratory of animal genetics Breeding and Reproduction of Shaanxi Province College of Animal Science and Technology Northwest A&F University

Background: N6-Methyladenosine(m^6A), the most prevalent modification in mammalian m RNA, plays important roles in numerous biological processes. Several m^6A associated proteins such as methyltransferase like 3(METTL3),methyltransferase like 14(METTL14), α-ketoglutarate-dependent dioxygenase Alk B homolog 5(ALKBH5) and YTH domain containing 2(YTHDC2) are involved in the regulation of spermatogenesis and oogenesis. However, the role of the first detected m^6A demethylase, fat mass and obesity associate protein(FTO), in germ cells remains *** of FTO roles in the regulation of germ cell fate will provide novel insights into the mammalian ***: Mouse GC-1 spg cells were treated with the ester form of meclofenamic acid(MA2) to inhibit the demethylase activity of FTO. The cellular m^6A and m^6Amlevel were analyzed through high performance liquid chromatography combined with tandem mass spectrometry(HPLC/MS-MS). The cell apoptosis was detected via TUNEL and flow cytometry. The cell proliferation was detected through Ed U and western blot. The m RNA level of core cyclin dependent kinases(CDKs) was quantified via q-PCR. RNA decay assay were performed to detect RNA stability. Dual fluorescence assay was conducted to study whether MA2 affects the expression of CDK2 dependent on the m^6A modification at 3’***: MA2 significantly increased the cellular m^6A level and down-regulated the expression of CDK1, CDK2, CDK6 and Cd C25 a, resulting in arrest of G1/S transition and decrease of cell proliferation. MA2 downregulated CDK2 m RNA stability. Additionally, mutation of the predicted m^6A sites in the Cdk2–3’UTR could mitigated the degradation of CDK2 m RNA after MA2 ***: MA2 affected CDKs expression through the m^6A-dependent m RNA degradation pathway, and thus repressed spermatogonial proliferation.

关键词： Cell cycle FTO Meclofenamic acid N6-methyladenosine Spermatogonial proliferation