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Spatial-temporal Distribution and Influencing Factors of Urban Carbon Emissions under the Background of Carbon Emission Reduction:A Case Study of Guangxi Autonomous Region

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Journal of Resources and ecology 2024年第4期15卷 870-879页

作者： ZHOU Xinran WANG Jinye HE Wen WEI Qingqing YANG Yihui School of Tourism and landscape Architecture Guilin University of TechnologyGuilinGuangxi 541006China Guangxi Key Laboratory of Karst plant Conservation and Restoration ecology Guangxi Institute of BotanyChinese Academy of SciencesGuilinGuangxi 541006China

Based on the panel data of Guangxi from 2005 to 2017,the spatiotemporal characteristics and determinants of urban carbon emissions in Guangxi were analyzed using the extended STIRPAT model and the Geographically and Temporally Weighted Regression(GTWR)*** main findings of our research can be summarized as *** the total carbon emissions of cities in Guangxi consistently increased from 2005 to 2014,the growth trend slowed after 2014,leading to a stabilization in the total *** addition,there are significant differences in the total carbon emissions among the *** central and northeastern regions have higher emissions,while the southwestern region has lower ***,there are variations in the degrees and directions of the impacts that factors have on carbon emissions among the different time periods and *** land use is a key factor driving carbon emissions,and it has a negative impact on most cities in ***,factors such as industrial structure,population urbanization,population concentration,and economic growth have significant positive effects on carbon emissions in *** influence of urban roads on carbon emissions is generally positive,while the degree of openness to the outside world and environmental regulations has relatively weaker impacts on *** summary,in order to promote the low-carbon transition of Guangxi and achieve high-quality development,the cities in Guangxi should implement differentiated urban carbon reduction strategies that are focused on optimizing urban land use and industrial structure.

关键词： urban carbon emissions GTWR model improved SPIRPAT model influencing factor Guangxi

Genome sequencing provides insights into Caprifoliaceae genome evolution and the mechanism underlying second blooming phenomenon in Heptacodium miconioides

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Horticultural plant Journal 2024年第3期10卷 897-910页

作者： Yueling Li Zhongshuai Sun Zexin Jin Junmin Li Zhejiang Provincial Key Laboratory of plant Evolutionary ecology and Conservation Taizhou UniversityTaizhouZhejiang 318000China institute of ecology Taizhou UniversityTaizhouZhejiang 318000China School of Life Sciences Taizhou UniversityTaizhouZhejiang 318000China

plants of the Caprifoliaceae family are widely cultivated worldwide as ornamental plants owing to their numerous,sweet-smelling,beautiful flowers and *** miconioides Rehd.,a member of the family,is endemic to eastern China and is cultivated as a popular ornamental plant in North America and European *** has a rather novel and beautiful trait of high horticultural value,that is,its sepals persist and enlarge,turning purplish ***,we report the chromosome-level genome assembly of *** to understand its evolution and floral *** 622.28 Mb assembled genome harbored a shared whole-genome duplication with a related species,Lonicera *** genomic analysis suggested that chromosome fission events following genome duplication underlie the unusual chromosome number of these two species,as well as chromosome fission of another five chromosomes in ***,giving rise to a haploid chromosome number of 14(versus 9 in ***).In addition,based on transcriptome and chloroplast genome analysis of 17 representative species in the Caprifoliaceae,we assumed that large structural variations in the chromosomes of *** were not caused by *** in the candidate genes of the MADS-box family were detected in the *** genome,including AP1-,AP3-,and SEPexpanded,which might underlie the sepal elongation and development in this *** current findings provided a critical resource for genome evolution studies in Caprifoliaceae and it was an example of how multi-omics data can elucidate the regulation of important ornamental traits.

关键词： Heptacodium miconioides Genome assembly Caprifoliaceae Chromosome fusion Sepal traits

Masculinizer gene controls sexual differentiation in Hyphantria cunea

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Insect Science 2024年第2期31卷 405-416页

作者： Xiaowei Li Huihui Liu Honglun Bi Yaohui Wang Jun Xu Sufang Zhang Zhen Zhang Ze Zhang Yongping Huang Laboratory of Evolutionary and Functional Genomics School of Life SciencesChongqing UniversityChongqingChina CAS Key Laboratory of Insect Developmental and Evolutionary Biology CAS Centerfor Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologyShanghaiChina Key Laboratory of Forest Protection Research Institute of Forest EcologyEnvironment and ProtectionChinese Academy of ForestryState Forestry AdministrationBeijingChina

The Masculinizer gene,Masc,encodes a lepidopteran-specific novel CCCHtype zinc finger protein,which controls sex determination and dosage compensation in Bombyx *** the potential application of it in pest control,it is necessary to investigate the function of Masc gene in Hyphantria cunea,a globally invasive forest *** the present study,we identified and functionally characterized the Masc gene,HcMasc,in *** analysis revealed that HcMASC contained the conserved CCCH-type zinc finger domain,nuclear localization signal,and male determining domain,in which the last was confirmed to be required for its masculinization in BmN cell ***,expression data showed that unlike male-biased expression in ***,Hc-Masc gene expresses in main all developmental stages or tissues in both *** regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9-based disruption of the common exons 1 and 3 of the HcMasc gene resulted in imbalanced sex ratio and abnormal external genitalia of both *** results suggest that the HcMasc gene is required for both male and female sexual differentiation and dosage compensation in *** and provide a foundation for developing better strategies to control this pest.

关键词： CRISPR/Cas9 dosage compensation Hyphantria cunea Masc sexual differentiation

Single-cell transcriptomic landscape of peripheral blood cells provides insights into adaptation of red-eared sliders(Trachemys scripta elegans)

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Integrative Zoology 2024年第3期19卷 468-479页

作者： Rui GUO Guangwei MA Xiaofei ZHAI Haitao SHI Jichao WANG Ministry of Education Key Laboratory for ecology of Tropical Islands Key Laboratory of Tropical Animal and Plant Ecology of Hainan ProvinceCollege of Life SciencesHainan Normal UniversityHaikouChina

Red-eared sliders(Trachemys scripta elegans),as one of the 100 most threatening aliens,have stronger immunity than the native species in response to environmental *** cells are an important component of immunity in the ***,the blood cell researches of turtle are still in the traditional blood cell classification and morphological structure ***,turtle granulocytes cannot be accurately identified using traditional ***-cell RNA sequencing techniques have been successfully implemented to study cells based on the mRNA expression patterns of each *** present study profiled the transcriptomes of peripheral blood cells in red-eared sliders to construct a single-cell transcriptional landscape of the different cell types and explored environmental adaptation mechanism from the perspective of *** 14 transcriptionally distinct clusters(platelets,erythrocytes1,erythrocytes2,CSF1R monocytes,POF1B monocytes,neutrophils,GATA2high basophils,GATA2low basophils,CD4 T cells,CD7 T cells,B cells,ACKR4 cells,serotriflin cells,and ficolin cells)were identified in the peripheral blood cells of the red-eared *** particular,a subtype of erythrocytes(erythrocytes1)that expressed immune signals was *** blood cells were grouped into three lineages:platelet,erythroid/lymphoid,and myeloid cell ***,based on differentiation trajectory and up-regulated gene expression,ACKR4 cells were newly identified as lymphocytes,and serotriflin and ficolin cells as *** single-cell transcriptional atlas of the peripheral blood cells in red-eared sliders provided in the present study will offer a comprehensive transcriptome reference for the exploration of physiological and pathological hematology in this species.

关键词： blood cells single-cell RNA-seq Trachemys scripta elegans

Does ash dieback affect the reproductive ecology of Fraxinus excelsior L.?

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Journal of Forestry Research 2024年第2期35卷 54-69页

作者： Anna‑Katharina Eisen Lisa Buchner Barbara Fussi Susanne Jochner‑Oette Physical Geography/landscape ecology and Sustainable Ecosystem Development Catholic University of Eichstätt-Ingolstadt85072 EichstättGermany Bavarian Office for Forest Genetics(AWG) 83317 TeisendorfGermany

Forest tree species reproduction is a key factor in maintaining the genetic diversity of future generations and the stability of forest *** ongoing ash dieback disease could affect the reproductive ecology of Fraxinus excelsior *** have a major impact on the quantity and quality of pollen and *** this study,we investigated pollen production and viability of pollen and seeds of ash trees with different health status from 2018 to *** were collected from 105 trees(pollen production),pollen from 125 trees(pollen viability),and seeds from 53 trees(seed quality)in two seed orchards and in one floodplain forest in southern *** all parameters were examined at every site every *** average pollen production per tree was estimated at 471.2±647.9 billion pollen *** addition,we found that a high number of inflorescences did not equate to high pollen production per *** production of healthy and diseased trees did not differ significantly,although only 47%of severely diseased male trees(vs.72%for healthy trees)produced *** regards to pollen viability,the TTC test showed an average viability of 73%±17%.Overall,there was a slight tendency for diseased trees to have less viable ***,a significant difference could only be calculated for trees in the floodplain *** percentage of germinable seeds in 2018 was 38%in the floodplain forest and 57%in one of the seed *** percentage of viable seeds(TTC test)ranged from 17 to 22%in the orchards in ***-viable seeds were usually heavily infested by *** general,seed quality was not significantly different between healthy and diseased *** results indicate that ash dieback affects flower formation and pollen viability but not pollen production or seed ***,the fact that hardly any flowering was observed,especially for trees that were seriously affected,suggests a negative effect of ash dieback on reproductive per

关键词： Common ash Seed orchard Floodplain forest Pollen production Viability TTC test Seed stratification Phenology

How plants sense and respond to osmotic stress

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Journal of Integrative plant Biology 2024年第3期66卷 394-423页

作者： Bo Yu Dai-Yin Chao Yang Zhao Shanghai Center for plant Stress Biology CAS Center for Excellence in Molecular Plant SciencesThe Chinese Academy of SciencesShanghai 200032China Key Laboratory of plant Carbon Capture The Chinese Academy of SciencesShanghai 200032China National Key Laboratory of plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologyThe Chinese Academy of SciencesShanghai 200032China University of Chinese Academy of Sciences Beijing 100049China

Drought is one of the most serious abiotic stresses to land *** sense and respond to drought stress to survive under water *** have studied how plants sense drought stress,or osmotic stress caused by drought,ever since Charles Darwin,and gradually obtained clues about osmotic stress sensing and signaling in *** stress is a physical stimulus that triggers many physiological changes at the cellular level,including changes in turgor,cell wall stiffness and integrity,membrane tension,and cell fluid volume,and plants may sense some of these stimuli and trigger downstream *** this review,we emphasized water potential and movements in organisms,compared putative signal inputs in cell wall-containing and cell wall-free organisms,prospected how plants sense changes in turgor,membrane tension,and cell fluid volume under osmotic stress according to advances in plants,animals,yeasts,and bacteria,summarized multilevel biochemical and physiological signal outputs,such as plasma membrane nanodomain formation,membrane water permeability,root hydrotropism,root halotropism,Casparian strip and suberin lamellae,and finally proposed a hypothesis that osmotic stress responses are likely to be a cocktail of signaling mediated by multiple *** also discussed the core scientific questions,provided perspective about the future directions in this field,and highlighted the importance of robust and smart root systems and efficient source-sink allocations for generating future high-yield stress-resistant crops and plants.

关键词： cell volume drought membrane tension osmotic stress turgor

Seasonal increase in nest defense,but not egg rejection,in a cuckoo host

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Avian Research 2024年第1期15卷 50-54页

作者： Bo Zhou Wei Liang Ministry of Education Key Laboratory for ecology of Tropical Islands Key Laboratory of Tropical Animal and Plant Ecology of Hainan ProvinceCollege of Life SciencesHainan Normal UniversityHaikou 571158China institute of Biological Resources Jiangxi Academy of ScienceNanchang 330096China

The interactions between avian brood parasites and their hosts provide an informative and easy-to-handle system for studying *** brood parasitism reduces the reproductive success of hosts,and thus,hosts have evolved anti-parasitic strategies,such as rejecting parasitic eggs and adopting aggressive nest defense strategies,to avoid the cost brought on by brood *** test whether host anti-parasitic strategies are adjusted with the risk of being parasitized when the breeding seasons of brood parasites and hosts are not synchronous,we conducted a field experiment assessing nest defense and egg recognition behaviors of the Isabelline Shrike(Lanius isabellinus),a host of the Common Cuckoo(Cuculus canorus).In the local area,the host Isabelline Shrike begins to breed in April,whereas the summer migratory Common Cuckoo migrates to the local area in May and begins to lay parasitic *** showed that nest defense behaviors of the Isabelline Shrike increases significantly after cuckoo arrival,showing higher aggressiveness to cuckoo dummies,with no significant difference in attack rates among cuckoo,sparrowhawk and dove dummies,but their egg rejection did not change *** results imply that Isabelline Shrikes may adjust their nest defense behavior,but not egg rejection behavior,with seasonality.

关键词： Aggression Anti-parasite strategy Egg rejection Mismatch cuckoo parasitism Nest defense

Optimized protoplast isolation and transfection with a breakpoint:accelerating Cas9/sgRNA cleavage efficiency validation in monocot and dicot

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aBIOTECH 2024年第2期5卷 151-168页

作者： Debasmita Panda Subhasis Karmakar Manaswini Dash Swagat Kumar Tripathy Priya Das Sagar Banerjee Yiping Qi Sanghamitra Samantaray Pradipta Kumar Mohapatra Mirza J.Baig Kutubuddin A.Molla ICAR National Rice Research institute CuttackOdisha 753006India Department of Botany Ravenshaw UniversityCuttackOdisha 753003India Department of plant Science and landscape Architecture University of MarylandCollege ParkMD 20742USA

The CRISPR-Cas genome editing tools are revolutionizing agriculture and basic biology with their simplicity and precision ability to modify target genomic ***-predicted guide RNAs(gRNAs)often fail to induce efficient cleavage at target *** target loci are inaccessible due to complex chromatin ***,there is no suitable tool available to predict the architecture of genomic target sites and their ***,significant time and resources are spent on performing editing experiments with inefficient *** in vitro-cleavage assay could provide a rough assessment of gRNA efficiency,it largely excludes the interference of native genomic *** in-vivo testing gives a proper assessment of the cleavage ability of editing reagents in a native genomic ***,we developed a modified protocol that offers highly efficient protoplast isolation from rice,Arabidopsis,and chickpea,using a sucrose gradient,transfection using PEG(polyethylene glycol),and validation of single guide RNAs(sgRNAs)cleavage efficiency of *** have optimized various parameters for PEG-mediated protoplast transfection and achieved high transfection efficiency using our protocol in both monocots and *** introduced plasmid vectors containing Cas9 and sgRNAs targeting genes in rice,Arabidopsis,and chickpea *** dual sgRNAs,our CRISPRdeletion strategy offers straightforward detection of genome editing success by simple agarose gel *** sequencing of PCR products confirmed the editing efficiency of specific ***,we demonstrated that isolated protoplasts can be stored for up to 24/48 h with little loss of viability,allowing a pause between isolation and *** high-efficiency protocol for protoplast isolation and transfection enables rapid(less than 7 days)validation of sgRNA cleavage efficiency before proceeding with stable *** isolation and transfection method can als

关键词： Protoplast Genome editing Transgene-free edited plants Rice Chickpea

Linkages among leaf nutrient concentration,resorption efficiency, litter decomposition and their stoichiometry to canopy nitrogen addition and understory removal in subtropical plantation

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Ecological Processes 2024年第2期13卷 90-102页

作者： Jawad Ali Shah Wenfei Liu Saif Ullah Honglang Duan Fangfang Shen Yingchun Liao Guomin Huang Jianping Wu Ministry of Education Key Laboratory for Transboundary Ecosecurity of Southwest China Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology and Institute of Biodiversity School of Ecology and Environmental Science Yunnan University Laboratory of Soil ecology and Health in Universities of Yunnan Province Yunnan University Jiangxi Key Laboratory for Restoration of Degraded Ecosystems & Watershed Ecohydrology Nanchang Institute of Technology Key Laboratory of Forest Cultivation in Plateau Mountain of Guizhou Province Institute for Forest Resources and Environment of Guizhou College of ForestryGuizhou University

Background The prevalence of understory removal and anthropogenic nitrogen(N) deposition has significantly altered the ecological processes of forest ecosystems at both regional and global scales. However, it remains a pressing challenge to understand how N deposition and understory removal affect leaf nutrient dynamics, nutrient resorption, litter decomposition, and their linkages for better managing forest ecosystems under nutrient imbalances induced by N enrichment. To address this research gap, a field manipulation experiment was carried out in a subtropical Cunninghamia lanceolata plantation with four treatments including: control(CK), canopy N addition(CN), understory removal(UR), and canopy N addition plus understory removal(CN × UR). Green and senesced leaf N and phosphorus(P) concentrations, N and P resorption efficiencies, litter decomposition, and their correlations were *** The results revealed that the average N concentrations of green early and late leaves in UR were increased by 6.61 and 18.89% compared to CK. UR had the highest whereas CN had the lowest P concentrations in green leaves across the two sampling seasons. Following this, UR, leaf type, season, and their interactions significantly affected leaf N, P, and N:P(P < 0.05). The highest leaf N resorption(32.68%) and P resorption efficiencies(63.96%) were recorded in UR. Litter decomposition was significantly retarded in UR(P < 0.01) relative to CN. The regression analysis demonstrated that leaf nutrient status was significantly interconnected with leaf nutrient resorption efficiencies. In addition, leaf nutrient dynamics were strongly correlated with litter nutrients, indicating that both were *** These findings can deepen our knowledge of biogeochemical cycling and reveal contrasting nutrientacquisition strategies on N and P limitation in response to UR and CN. Considering the P limitation, it is important to note that P was resorbed more efficiently, illustrating a

关键词： Nutrient resorption Nitrogen deposition Litter decomposition Stoichiometry Understory removal Subtropical plantation