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High-energy-density lithium manganese iron phosphate for lithium-ion batteries: Progresses, challenges, and prospects

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Journal of Energy Chemistry 2025年第1期100卷 1-17页

作者： Bokun Zhang Xiaoyun Wang Shuai Wang Yan Li Libo Chen Handong Jiao Zhijing Yu Jiguo Tu State Key Laboratory of Advanced Metallurgy University of Science and Technology Beijing Research institute for Intelligent Wearable systems The Hong Kong Polytechnic University institute of Advanced Structure Technology Beijing Institute of Technology

The soaring demand for smart portable electronics and electric vehicles is propelling the advancements in high-energy–density lithium-ion *** manganese iron phosphate (LiMnxFe1-xPO4) has garnered significant attention as a promising positive electrode material for lithium-ion batteries due to its advantages of low cost,high safety,long cycle life,high voltage,good high-temperature performance,and high energy *** LiMnxFe1-xPO4has made significant breakthroughs in the past few decades,there are still facing great challenges in poor electronic conductivity and Li-ion diffusion,manganese dissolution affecting battery cycling performance,as well as low tap *** review systematically summarizes the reaction mechanisms,various synthesis methods,and electrochemical properties of LiMnxFe1-xPO4to analyze reaction processes accurately and guide material ***,the main challenges currently faced are concluded,and the corresponding various modification strategies are discussed to enhance the reaction kinetics and electrochemical performance of LiMnxFe1-xPO4,including multi-scale particle regulation,heteroatom doping,surface coating,as well as microscopic morphology ***,in view of the current research challenges faced by intrinsic reaction processes,kinetics,and energy storage applications,the promising research directions are *** importantly,it is expected to provide key insights into the development of high-performance and stable LiMnxFe1-xPO4materials,to achieve practical energy storage requirements.

关键词： Lithium manganese iron phosphate High energy density Lithium-ion batteries Reaction mechanism Tap density

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Small extrachromosomal circular DNA in amyotrophic lateral sclerosis matter

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Neural Regeneration Research 2025年第5期20卷 1411-1413页

作者： Marcos J.Arauzo-Bravo Daniela Gerovska Matthias Schwab Alexandra Kretz Computational biology and systems Biomedicine Biodonostia Health Research InstituteSan SebastianSpain Basque Foundation for Science IKERBASQUEBilbaoSpainMax Planck Institute for Molecular BiomedicineComputational Biology and Bioinformatics GroupMünsterNorth Rhine-WestphaliaGermany Department of Cell biology and Histology Faculty of Medicine and NursingUniversity of Basque Country(UPV/EHU)LeioaSpain Department of Neurology Jena University HospitalJenaThuringiaGermany

Comprehensive studies identify motor neuron spectrum disorders including amyotrophic lateral sclerosis(ALS)as globally rising fatal disorders with the highest prevalence in aging populations,influenced by ethnicity and ancestry(GBD 2016 Motor Neuron Disease Colla borators,2018).While~10% of diagnoses involve a family history(fALS),most cases are considered sporadic(sALS).However,population-based studies suggest that even cases without a common index mutation impart heritability(Ryan et al.,2019),indicating a crucial role of rare and as yet unknown genetic denominators.

关键词： amyotrophic sclerosis

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Inter-chromosomal insertions into wild-type chromosomes induced by SCRaMbLE

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Frontiers of Chemical Science and Engineering 2024年第9期18卷 125-135页

作者： Sijie Zhou Junyanrui Li Xichen Cui Ying Wang Ying-Jin Yuan Frontiers Science Center for synthetic biology and Key Laboratory of systems Bioengineering(Ministry of Education) School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China Frontiers Research institute for synthetic biology Tianjin UniversityTianjin 300072China

Genomic rearrangements play a crucial role in shaping biological phenotypic diversity and driving species *** chromosome rearrangement and modification by LoxP-mediated evolution(SCRaMbLE)has been applied to explore large-scale genomic rearrangements,yet it has been observed that these rearrangements occur exclusively in genomic regions containing loxPsym ***,we found that SCRaMbLE of synthetic yeast harboring synthetic chromosome V and X can generate a variety of synthetic segment insertions into wild-type chromosomes,ranging from 1 to 300 ***,it was revealed that the novel insertions impacted the transcriptional level of neighboring regions and affected the production of exemplar pathway of ***,our results improve the understanding of the ability of SCRaMbLE to generate complex structural variations in nonsynthetic regions and provide a potential model to explore genomic transposable events.

关键词： genomic rearrangements synthetic yeast genome SCRaMbLE inter-chromosomal insertions synthetic biology

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Dissecting PCNA function with a systematically designed mutant library in yeast

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Journal of Genetics and Genomics 2019年第6期46卷 301-313页

作者： Qingwen Jiang Weimin Zhang Chenghao Liu Yicong Lin Qingyu Wu Junbiao Dai Center for synthetic and systems biology School of Life Sciences Tsinghua University Beijing 100084 China Shenzhen Key Laboratory of synthetic Genomics and Center for synthetic Genomics Institute of Synthetic Biology Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Shenzhen 518055 China Department of biology. Brandeis University. Massachusetts 02454 USA

Proliferating cell nuclear antigen (PCNA), encoded by POL30 in Saccharomyces cerevisiae, is a key component of DNA metabolism. Here, a library consisting of 304 PCNA mutants was designed and constructed to probe the contribution of each residue to the biological function of PCNA. Five regions with elevated sen sitivity to DNA damaging reagents were identified using high-throughput phe no type screening. Using a series of genetic and biochemical analyses, we demonstrated that one particular mutant, K168A, has defects in the DNA damage tolerance (DDT) pathway by disrupting the interaction between PCNA and Rad5. Subsequent domain analysis showed that the PCNA-Rad5 interaction is a prerequisite for the function of Rad5 in DDT. Our study not only provides a resource in the form of a library of versatile mutants to study the functions of PCNA, but also reveals a key residue on PCNA (K168) which highlights the importance of the PCNA-Rad5 interaction in the template switching (TS) pathway.

关键词： Mutagenesis High-throughput DNA damage tolerance Template switching Genetic interaction

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Diatom-inspired multiscale mineralization of patterned protein–polysaccharide complex structures

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National Science Review 2021年第8期8卷 186-197页

作者： Ke Li Yingfeng Li Xinyu Wang Mengkui Cui Bolin An Jiahua Pu Jintao Liu Boyang Zhang Guijun Ma Chao Zhong School of Physical Science and Technology Shanghai Tech University Center for Materials synthetic biology Shenzhen Institute of Synthetic BiologyShenzhen Institutes of Advanced TechnologyChinese Academy of Sciences CAS Key Laboratory of Quantitative Engineering biology Shenzhen Institute of Synthetic BiologyShenzhen Institutes of Advanced TechnologyChinese Academy of Sciences

Marine diatoms construct their hierarchically ordered, three-dimensional(3D) external structures called frustules through precise biomineralization processes. Recapitulating the remarkable architectures and functions of diatom frustules in artificial materials is a major challenge that has important technological implications for hierarchically ordered composites. Here, we report the construction of highly ordered,mineralized composites based on fabrication of complex self-supporting porous structures—made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin—and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2and Ga2O3. Subsequently, using sugar cubes as templates, we demonstrate that 3D fabricated porous structures can become colonized by engineered bacteria and can be functionalized with highly photoreactive minerals,thereby enabling co-localization of the photocatalytic units with a bacteria-based hydrogenase reaction for a successful semi-solid artificial photosynthesis system for hydrogen evolution. Our study thus highlights the power of coupling genetically engineered proteins and polysaccharides with biofabrication techniques to generate hierarchically organized mineralized porous structures inspired by nature.

关键词： biomimetic mineralization patterned porous structure genetic engineering amyloid protein artificial photosynthesis

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synthetic biology in the UK-An outline of plans and progress

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synthetic and systems Biotechnology 2016年第4期1卷 243-257页

作者： L.J.Clarke R.I.Kitney UK synthetic biology Leadership Council UK Department of BioEngineering Imperial CollegeUK Faculty of Life Sciences University of ManchesterUK BionerG UK EPSRC National Centre for synthetic biology and Innovation UK institute of systems and synthetic biology UK BioMedical systems Engineering Imperial CollegeUK BioMedical Systems EngineeringImperial CollegeUK

synthetic biology is capable of delivering new solutions to key challenges spanning the bioeconomy,both nationally and *** this significant potential and the associated need to facilitate its translation and commercialisation the UK government commissioned the production of a national synthetic biology Roadmap in 2011,and subsequently provided crucial support to assist its *** infrastructural investments have been made,and important strides made towards the development of an effectively connected community of practitioners and interest groups.A number of synthetic biology Research Centres,DNA Synthesis Foundries,a Centre for Doctoral Training,and an Innovation Knowledge Centre have been established,creating a nationally distributed and integrated network of complementary facilities and *** UK synthetic biology Leadership Council published a UK synthetic biology Strategic Plan in 2016,increasing focus on the processes of translation and *** 50 start-ups,SMEs and larger companies are actively engaged in synthetic biology in the UK,and inward investments are starting to *** these initiatives provide an important foundation for stimulating innovation,actively contributing to international research and development partnerships,and helping deliver useful benefits from synthetic biology in response to local and global needs and challenges.

关键词： synthetic biology Engineering biology Biodesign Roadmap Responsible Research and Innovation(RRI) Innovation and Knowledge Centre(IKC) synthetic biology Leadership Council(SBLC) Bioeconomy

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Identification of crucial roles of transcription factor IhfA on high production of free fatty acids in Escherichia coli

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synthetic and systems Biotechnology 2024年第1期9卷 144-151页

作者： Lixia Fang Ziyi Han Xueru Feng Xueyan Hao Mengxiao Liu Hao Song Yingxiu Cao Frontiers Science Center for synthetic biology and Key Laboratory of systems Bioengineering(Ministry of Education) School of Chemical Engineering and TechnologyTianjin UniversityTianjin300072China Frontiers Research institute for synthetic biology Tianjin UniversityChina

Transcription factor engineering has unique advantages in improving the performance of microbial cell factories due to the global regulation of gene *** analyses and reverse engineering enable learning and subsequent incorporation of novel design strategies for further ***,we identify the role of the global regulator IhfA for overproduction of free fatty acids(FFAs)using CRISPRi-facilitated reverse engineering and cellular physiological *** the differentially expressed genes in the ihfAL􀀀strain,a total of 14 beneficial targets that enhance FFAs production by above 20% are identified,which involve membrane function,oxidative stress,and *** membrane-related genes,the engineered strains obtain lower cell surface hydrophobicity and increased average length of membrane lipid *** oxidative stress-related genes,the engineered strains present decreased reactive oxygen species(ROS)*** gene modulations enhance cellular robustness and save cellular resources,contributing to FFAs *** study provides novel targets and strategies for engineering microbial cell factories with improved FFAs bioproduction.

关键词： Free fatty acids CRISPRi ihfA Cell membrane ROS

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Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li Dongqing Sun Shaliu Fu Lei Gu Xin Shao Qinqin Wang Xin Dong Bin Duan Feiyang Xing Jun Wu Minmin Xiao Fangqing Zhao Jing-Dong J.Han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research institute of Intelligent Computing Zhejiang Lab Shanghai Research institute for Intelligent Autonomous systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

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Soma-germline communication drives sex maintenance in the Drosophila testis

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National Science Review 2024年第8期11卷 98-113页

作者： Rui Zhang Peiyu Shi Shuyang Xu Zhe Ming Zicong Liu Yuanyuan He Junbiao Dai Erika Matunis Jin Xu Qing Ma Shenzhen Key Laboratory of synthetic Genomics Guangdong Provincial Key Laboratory of Synthetic GenomicsKey Laboratory of Quantitative Synthetic Biology Shenzhen Institute of Synthetic Biology Shenzhen Institute of Advanced Technology Chinese Academy of Sciences State Key Laboratory of Biocontrol School of Life Sciences Sun Yat-Sen University Department of Cell biology Johns Hopkins University School of Medicine

In adult gonads,disruption of somatic sexual identity leads to defective gametogenesis and ***,the underlying mechanisms by which somatic signals regulate germline cells to achieve proper gametogenesis remain *** our previous study,we introduced the chinmoSex Transformation(chinmoST)mutant Drosophila testis phenotype as a valuable model for investigating the mechanisms underlying sex *** chinmoSTtestes,depletion of the Janus Kinase-Signal Transducer and Activator of Transcription downstream effector Chinmo from somatic cyst stem cells（CySCs） feminizes somatic cyst cells and arrests germline ***,we use single-cell RNA sequencing to uncover chinmoST-specific cell populations and their transcriptomic changes during sex *** analysis of intercellular communication networks between wild-type and chinmoSTtestes revealed disruptions in several soma-germline signaling pathways in ***,the insulin signaling pathway exhibited significant enhancement in germline stem cells（GSCs）.Chinmo cleavage under targets and tagmentation（CUT&Tag） assay revealed that Chinmo directly regulates two male sex determination factors,doublesex（dsx） and fruitless（fru）,as wellas Ecdysone-inducible gene L2（ImpL2）,a negative regulator of the insulin signaling *** genetic manipulations confirmed that the impaired gametogenesis observed in chinmoSTtestes was partly contributed by dysregulation of the insulin signaling *** summary,our study demonstrates that somatic sex maintenance promotes normal spermatogenesis through Chinmo-mediated conserved sex determination and the insulin signaling *** work offers new insights into the complex mechanisms of somatic stem cell sex maintenance and soma-germline communication at the single-cell ***,our discoveries highlight the potential significance of stem cell sex instability as a novel mechanism contributing to testicular tumorigene

关键词： Drosophila testis somatic stem cell sex conversion single-cell sequencing soma-germline communication insulin signaling

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Biotransformation of ethylene glycol by engineered Escherichia coli

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synthetic and systems Biotechnology 2024年第3期9卷 531-539页

作者： Wenlong Yan Xinhua Qi Zhibei Cao Mingdong Yao Mingzhu Ding Yingjin Yuan Frontiers Science Center for synthetic biology and Key Laboratory of systems Bioengineering(Ministry of Education) School of Chemical Engineering and TechnologyTianjin UniversityTianjin300072China Frontiers Research institute for synthetic biology Tianjin UniversityTianjin300072China

There has been extensive research on the biological recycling of PET waste to address the issue of plastic waste pollution,with ethylene glycol(EG)being one of the main components recovered from this ***,finding ways to convert PET monomer EG into high-value products is crucial for effective PET waste *** this study,we successfully engineered Escherichia coli to utilize EG and produce glycolic acid(GA),expecting to facilitate the biological recycling of PET *** engineered ***,able to utilize 10 g/L EG to produce 1.38 g/L GA within 96 h,was initially ***,strategies based on overexpression of key enzymes and knock-out of the competing pathways are employed to enhance EG utilization along with GA *** engineered ***,characterized by the highest GA production titer and substrate conversion rate,was *** GA titer increased to 5.1 g/L with a yield of 0.75 g/g EG,which is the highest level in the shake flake *** level analysis and metabolomic analysis were then conducted,revealing that overexpression of key enzymes and knock-out of the competing pathways improved the metabolic flow in the EG *** improved metabolic flow also leads to accelerated synthesis and metabolism of amino acids.

关键词： PET biological recycling Ethylene glycol utilization Glycolic acid production Escherichia coli synthetic biology Metabolomics analysis

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