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A METHOD FOR ESTABLISHING AN ANIMAL MODEL OF LIKE-AUDITARY NEUROPATHY

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Journal of Otology 2014年第1期9卷 48-51页

作者： GUO Weiwei CHEN Peipei WEI Xiaoquan YANG Shiming Department of otolaryngology-Head and Neck Surgery Institute of Oto-Neurobiology Centre Otolaryngology Chinese PLA General Hospital Department of otolaryngology-Head and Neck Surgery Nanjing Children's Hospital Nanjing Medical University

Objective: To establish an animal model of like-auditory neuropathy in neonatal rat. Methods The ani-mals were injected with phenylhydrazine hydrochloride or saline at 7-day of age. ABR and DPOAE were performed to assess the auditory function. The cochlea basilar membrane stretched preparation and cochlear frozen sections were prepared for immunohistochemical staining to examine the morphological change of hair cells and spiral ganglion cells (SGNs). Results At 7-day age the ABR waveI, III, V, latencies andI-III,I-V IWIs in the experimental group were significantly prolonged compared with those in the control group. The ABR thresholds were also elevated in the experimental group. We found there is no significant differ-ence in DPOAE in phenylhydrazine hydrochloride exposure group compare to control group. The cochlear hair cells showed no signs of loss in both group, but the total number of neurofilaments positive cells in SGNs were significantly reduced in the phenylhydrazine treated animals. Conclusion Our study suggests that phenylhydrazine hydrochloride can change the auditory function and induce peripheral nerve pathology by targeted mainly the SGNs in neonatal rat.

关键词： Animal model Auditory brainstem response Distortion-product otoacoustic emission auditory neuropathy

ISOLATION AND INDUCTION OF DIFFERENTIATION OF SEINE ADIPOSE-DERIVED MESENCHYMAL STEM CELLS

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Journal of Otology 2014年第2期9卷 101-105页

作者： MA Yueying YUAN Shuolong ZHANG yue XU liangwei GUO Weiwei ZHAO Lidong ZHAI suoqiang YANG Shiming Department of otolaryngology-Head and Neck Surgery Institute of OtolaryngologyChinese PLA General Hospital Department of otolaryngology Head Neck SurgeryBeijing Friendship HospitalCapital Medical University Medical School of Nankai University

Objectives To establish a method for high yield mesenchymal stem cells collection, as well as a culture method for iden- tifying mesenchymal stem cells from the swine adipose-derived mesenchymal stem cell （ADMSC）. Methods Swine AD- MSCs were isolated from fat tissue with collagenase, followed by induction of differentiation to osteogenic, adipogenic and chondrogrnic cells. The survival curve of the ADMSC at the 37℃ and 38℃ were measured using WST-1 Cell Proliferation Assay Reagent. Result ADMSCs isolated with collagenase from swine neck fat tissue generated a stable uniform appearance af- ter the second generation. The passage period was five days. ADMSC could differentiate into osteogenic, adipogenic or chon- drogrnic cells under different culture conditions. The highest growth rate was achieved at 38℃in this study. Conclusion Swine ADMSCs have the potential to differentiate into osteogenic, adipogenic or chondrogrnic cells, and they may be appropriate for transplantation for both research and clinical purpose.

关键词： Adipose-derived mesenchymal stem cell Differentiation Transplantation Osteogenic cell, Adipogenic cell,Chondrogrnic cell

(-)-Epigallocatechin-3-gallate protects spiral ganglion neurons against amikacin-induced apoptosis

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Neural Regeneration Research 2011年第27期6卷 2112-2117页

作者： Qianghe Liu Dinghua Xie Xinming Yang Department of otolaryngology-Head Affiliated Hospital of Guilin Medical College Guilin 541001 Guangxi Zhuang Autonomous RegionChina Department of otolaryngology-Head ＆ Neck Surgery of the Second Xiangya Hospital and Hearing Science Research institute Central SouthUniversity Changsha 410011 Hunan Province China

Morphology of spiral ganglion neurons （SGNs） in Sprague-Dawley rats before and after amikacin treatment was observed by transmission electron microscopy. Amikacin induced cochlear SGN apoptosis. Immunohistochemical staining and RT-PCR revealed a decrease in Bcl-2 protein ex-pression, and an increase in Bax protein, caspase-3 protein and caspase-6 mRNA expression fol-lowing amikacin treatment. （-）-Epigallocatechin-（3）-gallate （EGCG） inhibited SGN Bax protein, caspase-3 protein and caspase-6 mRNA expression, and enhanced Bcl-2 protein expression, thereby decreasing SGN apoptosis. Results demonstrated that EGCG can protect SGNs against amikacin-induced injury.

关键词： amikacin apoptosis (-)-epigallocatechin-3-gallate Bcl-2 Bax caspase-3 caspase-6 spiral ganglion neuron neural regeneration

Excitotoxic Effects of Glutamate on Cochlear Organotypic Cultures

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2015年第1期35卷 117-121页

作者：胡姚周柳青鲁海涛袁琨龚树生 Department of otolaryngology-Head and Neck Surgery Wuhan Central Hospital Department of Otorhinolaryngology Union Hospital Tongji Medical College Huazhong University of Science and Technology Department of Otorhinolaryngology Jingzhou Central Hospital Department of otolaryngology-Head and Neck Surgery Affiliated Beijing Tongren Hospital of Capital Medical University and Beijing Institute of Otolaryngology

Glutamate（Glu） is the major afferent excitatory neurotransmitter in the auditory system, and excessive Glu may play an important role in cochlear dysfunction. It is unclear how excessive Glu plays roles in cochlear dysfunction in cochlear organotypic cultures. In this study neonatal rat cochlear organotypic cultures were prepared, and then the cochlear tissues were incubated with a new medium containing specific concentrations of Glu（0.1, 0.5, 1, 10 or 20 mmol/L） for 24 h, or incubated with the medium containing a concentration of 20 mmol/L Glu for 6, 12, 24 or 72 h, respectively. It was found that when the cochlear tissues were cultured for 24 h, the inner hair cells（IHCs） were damaged at the concentration of 0.5 mmol/L Glu, and with the increases of the concentrations, the injury was gradually aggravated, and 20 mmol/L Glu resulted in the significant loss of IHCs. In the 20 mmol/L Glu groups, the stereocilia bundles were missing or disarrayed on a few IHCs after culture for 6 h and the damage effect was time-dependent. The missing of IHCs was more significant in the basal turn of the cochlea than in the middle turn of the cochlea under the same concentration of Glu exposure. These results suggest that excessive exogenous Glu affects the morphology of IHCs, but not affects the outer hair cells（OHCs） in cochlear organotypic cultures, and the excitotoxic effects are different on IHCs of different parts of the cochlea under the same concentration of Glu exposure.

关键词： cochlear organotypic culture excitotoxic effects glutamate

A hypothesis study on bionic active noise reduction of auditory organs

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Military Medical Research 2018年第2期5卷 178-182页

作者： Qing-Qing Jiang Ning Yu Shi-Ming Yang Department of otolaryngology Head and Neck Surgery Institute of OtolaryngologyChinese PLA General HospitalKey Laboratory of Hearing Impairment ScienceMinistry of EducationBeijing Key Laboratory of Hearing Impairment Prevention and TreatmentBeijing 100853China

Background:Noise exposure can lead to hearing loss and multiple system *** various forms of noise exist in our living environments,and our auditory organs are very sensitive to acoustic stimuli,it is a challenge to protect our hearing system in certain noisy *** of the hypothesis:Herein,we propose that our hearing organ could serve as a noise eliminator for high intensity noise and enhance acoustic signal processing abilities by increasing the signal-noise *** suprathreshold signals,the hearing system is capable of regulating the middle ear muscles and other structures to actively suppress the sound level to a safe *** the hypothesis:To test our hypothesis,both mathematic model analyses and animal model studies are *** on a digital 3D reconstructed model,every structure in the auditory system can be analyzed and tested for its contribution to the process of noise *** manufactured by this bionic method could be used and verified in animal models and ***:By mimicking the noise-reduction effect of the sophisticated structures in the hearing system,we may be able to provide a model that establishes a new active-sound-suppression *** innovative method may overcome the limited capabilities of current noise protection options and become a promising possibility for noise prevention.

关键词： Active Noise reduction Bionic Hearing loss

Evaluation of Vestibular Function in Diagnosis of Vestibular Schwannomas

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Current Medical Science 2021年第4期41卷 661-666页

作者： Xue-qing ZHANG Tai-sheng CHEN Wei WANG Department of Otorhinolaryngology Head and Neck Surgery Tianjin First Central HospitalTianjin300192China institute of otolaryngology of Tianjin Tianjin300192China Key Laboratory of Auditory Speech and Balance Medicine Tianjin300192China Key Clinical Discipline of Tianjin(otolaryngology) Tianjin300192China otolaryngology Clinical Quality Control Centre Tianjin300192China

Vestibular schwannomas(VS)are benign tumors of the vestibular *** common first symptoms are hearing loss and tinnitus,followed by imbalance,vertigo,and facial nerve *** subjective symptoms of VS patients are not consistent with the severity of vestibular lesions and the results of vestibular tests,which often interfere with clinicians’***,the main screening and diagnostic methods for VS are audiometry and magnetic resonance imaging(MRI),ignoring the evaluation of vestibular function at the source of pathological *** the development and improvement of vestibular evaluation technology and its wide application in the clinic,modern vestibular examination technology can reflect the severity and frequency of vestibular lesions and compensation from multiple perspectives,providing an objective basis for the diagnosis and treatment of vestibular *** this report,we review the results and characteristics of vestibular tests in VS patients and further clarify the clinical value of vestibular function assessment in the diagnosis and treatment of VS.

关键词： vestibular schwannomas vestibular function test vestibular compensation vertigo

The progenitors of inner ear hair cells and their regulating genes

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Journal of Otology 2010年第1期5卷 34-39页

作者： SHU Wei-ning ZHAO Li-dong ZHANG Xiao-bing YANG Shi-ming Department of otolaryngology Head and Neck Surgery Institute of OtolaryngologyChinese PLA General HospitalBeijing 100853China The First Affiliated Hospital of Lanzhou University Lanzhou 730000China

Hair cells in the mammalian inner ear are very fragile and are often injured as a result of acoustic trauma or exposure to ototoxic drugs (cisplatin, aminoglycosides, etc) [1]. In amphibians and birds, spontaneous

关键词： Hair cell Regeneration Progenitor cell Inner ear

Understanding auditory neuropathy spectrum disorder： a system- atic review in transgenic mouse models

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Science China(Life Sciences) 2016年第5期59卷 480-486页

作者： Li Wang Jing Guan Hongyang Wang Lan Lan Qiujing Zhang Liang Zong Wan Du Wenping Xiong Fengjiao Li Kaiwen Wu Dayong Wang Qiuju Wang institute of otolaryngology Chinese PLA General Hospital Medical School of Chinese PLA Beijing 100853 China Medical College Nankai University Tianjin 300071 China

Auditory neuropathy spectrum disorder is a unique group of hearing dysfunctions characterized by preserved outer hair cell function and abnormal neural conduction of the auditory pathway. However, the pathogenic mechanism underlying this disorder is not clear. We therefore performed a systematic review of genetic mouse models with different gene mutations to provide a valuable tool for better understanding of the process and the possible molecular mechanisms. Of the 18 articles retrieved, nine met the required criteria. All biochemical, histological, and electrophysiological results were recorded for each of the mouse models, as was the transgenic technology. This review provides a summary of different mouse models that may play an important role in the diagnosis and management of auditory neuropathy spectrum disorder in the future.

关键词： auditory neuropathy spectrum disorder transgenic mouse models pathogenesis systematic review

Outcome measures following tele-rehabilitation and conventional face to face rehabilitation in paediatric cochlear implant users during COVID-19 pandemic:A pilot study in a tertiary care setup

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Journal of Otology 2022年第1期17卷 31-38页

作者： Himanshu Verma Banumathy N Roshani Mishra Naresh K.Panda Department of otolaryngology and Head&Neck Surgery Post Graduate Institute of Medical Education and ResearchChandigarh160012India

Background:Following the COVID-19 pandemic,majority of paediatric cochlear implantees(CI)lost follow ups for rehabilitation and tele-therapy was *** study thus compared the outcome measures of paediatric CI users on tele-therapy versus conventional face to face therapy following COVID-19 ***:Twenty seven unilateral paediatric cochlear implantees in the age range of 2e11 years were divided into two groups based on the therapy modality,viz,tele-and face-to-face *** on the hearing age,participants were further divided into three groups,viz,0e2,2e4,and greater than four years.A complete the test battery comprising Integrated Scales of Development,Speech Intelligibility Rating scale,and Revised Categorical Auditory Perception were *** speech&language test battery was performed prior to initiating the rehabilitation and post 12 months of ***:Results of the present study revealed that conventional rehabilitation had better outcomes compared to *** rate of progress after one year of rehabilitation with respect to hearing-age showed a significant difference for the hearing-age group of 0e2 years across the domains of audition,speech and ***:The present study indicates that conventional method of the speech-language and auditory rehabilitation is far better compared to the tele rehabilitation services especially for those visiting tertiary care hospitals as most of them belong to lower and middle socioeconomic *** the results,it can be delineated that with lesser hearing experience,paediatric CI users always need to initially enroll for conventional therapy for better speech-language and auditory outcomes.

关键词： Cochlear implant Tele-therapy Conventional therapy Language outcomes Speech&language rehabilitation