检索结果-南通市图书馆

The overexpression of Rps14 in Lgr5+progenitor cells promotes hair cell regeneration in the postnatal mouse cochlea

Engineered Regeneration 2023年第3期4卷 328-336页

作者： Jingru Ai Pei Jiang Jingyuan Huang Hairong Xiao Yanqin Lin Mingchen Dai Yintao Wang Yuan Zhang Mengyao Tian Renjie Chai Shasha Zhang State Key Laboratory of Digital Medical Engineering Department of Otolaryngology Head and Neck SurgeryZhongda HospitalSchool of Life Sciences and TechnologyAdvanced Institute for Life and HealthJiangsu Province High-Tech Key Laboratory for Bio-Medical ResearchSoutheast UniversityNanjing 210096China Department of otolaryngology Head and Neck Surgery Affiliated Drum Tower Hospital of Nanjing University Medical SchoolJiangsu Provincial Key Medical Discipline(Laboratory)NanjingChina Research institute of otolaryngology NanjingChina Co-Innovation Center of Neuroregeneration Nantong UniversityNantong 226001China Department of otolaryngology Head and Neck Surgery Sichuan Provincial People’s HospitalUniversity of Electronic Science and Technology of ChinaChengduChina institute for Stem Cell and Regeneration Chinese Academy of ScienceBeijingChina Beijing Key Laboratory of Neural Regeneration and Repair Capital Medical UniversityBeijing 100069China

Sensory hair cells(HCs)in the cochlea cannot regenerate spontaneously in adult mammals after being damaged by external or genetic ***,several genes and signaling pathways are reported to induce cochlear HC regeneration at the early neonatal ***14 encodes a ribosomal protein that is involved in the regulation of cell differentiation and proliferation in ***,its roles in the cochlea have not been reported in ***,we specifically overexpressed Rps14 in Lgr5+progenitor cells in the newborn mouse cochlea and found that Rps14 conditional overexpression(cOE)mice had significantly increased the ectopic HCs,including inner and outer *** further explored the source of these ectopic HCs and found no EdU+supporting cells observed in the Rps14 cOE *** lineage tracing results,on the other hand,revealed that Rps14 cOE mice had significantly more tdTomato+HCs in their cochleae than control *** results indicated that regenerated HCs by cOE of Rps14 are most likely derived from inducing the direct trans-differentiation of Lgr5+progenitor cells into ***,real-time qPCR results suggested that the transcription factor genes Atoh1 and Gfi1,which are important in regulating HC differentiation,were upregulated in the cochlear basilar membrane of Rps14 cOE *** summary,this study provides in vivo evidence that,in the postnatal mouse cochlea,Rps14 is a potential gene that can promote the spontaneous trans-differentiation of Lgr5+progenitor cells into *** gene may one day be exploited as a therapeutic target for treating hearing loss.

关键词： Rps14 Lgr5+progenitor cells Ectopic hair cells Regeneration Proliferation Trans-differentiation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The single-cell transcriptomic landscape of the topological differences in mammalian auditory receptors

引用

Science China(Life Sciences) 2024年第11期67卷 2398-2410页

作者： Xiangyu Ma Xin Chen Yuwei Che Siyao Zhu Xinlin Wang Shan Gao Jiheng Wu Fanliang Kong Cheng Cheng Yunhao Wu Jiamin Guo Jieyu Qi Renjie Chai State Key Laboratory of Bioelectronics Department of Otolaryngology Head and Neck SurgeryZhongda HospitalSchool of Life Sciences and TechnologyAdvanced Institute for Life and HealthJiangsu Province High-Tech Key Laboratory for Bio-Medical ResearchSoutheast UniversityNanjing 210096China School of Engineering Vanderbilt UniversityNashville 37240USA Department of otolaryngology-Head and Neck Surgery Affiliated Drum Tower Hospital of Nanjing University Medical SchoolJiangsu Provincial Key Medical Discipline(Laboratory)Nanjing 210096China Research institute of Otorhinolaryngology Nanjing 210096China Department of Neurology Aerospace Center HospitalSchool of Life SciencesBeijing Institute of TechnologyBeijing 100081China Advanced Technology Research institute Beijing Institute of TechnologyBeijing 100081China Co-innovation Center of Neuroregeneration Nantong UniversityNantong 226001China Department of otolaryngology Head and Neck Surgery Sichuan Provincial People’s HospitalUniversity of Electronic Science and Technology of ChinaChengdu 610072China Southeast University Shenzhen Research institute Shenzhen 518063China

Mammalian hair cells(HCs)are arranged spirally along the cochlear axis and correspond to different frequency *** as primary sound detectors,HCs spatially segregate component frequencies into a topographical *** display significant diversity in anatomical and physiological characteristics,yet little is known about the organization of the cochleotopic map of HCs or the molecules involved in this *** single-cell RNA sequencing,we determined the distinct molecular profiles of inner hair cells and outer hair cells,and we identified numerous position-dependent genes that were expressed as *** identified genes such as Ptn,Rxra,and Nfe2l2 were found to be associated with *** employed the SCENIC algorithm to predict the transcription factors that potentially shape these tonotopic ***,we confirmed that Nfe2l2,a tonotopy-related transcription factor,is critical in mice for sensing low-tomedium sound frequencies in *** analysis of cell-cell communication revealed potential receptor-ligand networks linking inner hair cells to spiral ganglion neurons,including pathways such as BDNF-Ntrk and PTN-Scd4,which likely play essential roles in tonotopic ***,these findings suggest that molecular gradients serve as the organizing principle for maintaining the selection of sound frequencies by HCs.

关键词： tonotopic axis hair cell auditory receptor scRNA-seq

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

MUSIC AND COCHLEAR IMPLANTS

引用

Journal of Otology 2013年第1期8卷 32-38页

作者： Mao Yitao Xu Li Department of otolaryngology- Head and Neck Surgery Institute of OtologyThe Second Xiangya HospitalCentral South University School of Rehabilitation and Communication Sciences Ohio University

Currently, most people with modern multichannel cochlear implant systems can understand speech in qui-et environment very well. However, studies in recent decades reported a lack of satisfaction in music percep-tion with cochlear implants. This article reviews the literature on music ability of cochlear implant users by presenting a systematic outline of the capabilities and limitations of cochlear implant recipients with regard to their music perception as well as production. The review also evaluates the similarities and differences be-tween electric hearing and acoustic hearing regarding music perception. We summarize the research results in terms of the individual components of music (e.g., rhythm, pitch, and timbre). Finally, we briefly intro-duce the vocal singing of prelingually-deafened children with cochlear implants as evaluated by acoustic measures.

关键词： Music perception vocal singing cochlear implants

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Reproductive management through integration of PGD and MPS-based noninvasive prenatal screening/diagnosis for a family with GJB2-associated hearing impairment

引用

Science China(Life Sciences) 2015年第9期58卷 829-838页

作者： XIONG WenPing WANG DaYong GAO Yuan GAO Ya WANG HongYang GUAN Jing LAN Lan YAN JunHao ZONG Liang YUAN Yuan DONG Wei HUANG SeXin WU KeLiang WANG YaoShen WANG ZhiLi PENG HongMei LU YanPing XIE LinYi ZHAO Cui WANG Li ZHANG QiuJing GAO Yun LI Na YANG Ju YIN ZiFang HAN Bing WANG Wei CHEN Zi-Jiang WANG QiuJu Department of otolaryngology-Head and Neck Surgery Chinese PLA Institute of Otolaryngology Chinese PLA General Hospital Center for Reproductive Medicine Shandong Provincial Hospital Affiliated to Shandong University National Research Center for Assisted Reproductive Technology and Reproductive Genetics BGI-Shenzhen Department of Ultrasonography Chinese PLA General Hospital Department of Obstetrics and Gynecology Chinese PLA General Hospital The Key laboratory for Reproductive Endocrinology of Ministry of Education

A couple with a proband child of GJB2 （encoding the gap junction protein connexin 26）-associated hearing impairment and a previous pregnancy miscarriage sought for a reproductive solution to bear a healthy child. Our study aimed to develop a cus- tomized preconception-to-neonate care trajectory to fulfill this clinical demand by integrating preimplantation genetic diagno- sis （PGD）, noninvasive prenatal testing （NIPT）, and noninvasive prenatal diagnosis （N1PD） into the strategy. Auditory and ge- netic diagnosis of the proband child was carried out to identify the disease causative mutations. The couple then received in-vitro-fertilization treatment, and eight embryos were obtained for day 5 biopsy. PGD was performed by short-tandem-repeat linkage analysis and Sanger sequencing of GJB2 gene. Transfer of a GJB2c.235delC heterozygous embryo resulted in a sin- gleton pregnancy. At the 13th week of gestation, genomic DNA （gDNA） from the trio family and cell-free DNA （cfDNA） from maternal plasma were obtained for assessment of fetal chromosomal aneuploidy and GJB2 mutations. NIPT and NIPD showed the absence of chromosomal aneuploidy and GJB2-associated disease in the fetus, which was later confirmed by inva- sire procedures and postnatal genetic/auditory diagnosis. This strategy successfully prevented the transmission of hearing im- pairment in the newborn, thus providing a valuable experience in reproductive management of similar cases and potentially other monogenic disorders.

关键词： preimplantation genetic diagnosis(PGD) noninvasive prenatal testing(NIPT) noninvasive prenatal diagnosis(NIPD) GJB2(encoding the

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Nonsense mutations in the PAX3 gene cause Waardenburg syndrome type I in two Chinese patients

引用

Chinese Medical Journal 2007年第1期120卷 46-49页

作者： YANG Shu-zhi CAO Ju-yang ZHANG Rui-ning LIU Li-xian LIU Xin ZHANG Xin KANG Dong-yang LI Mei HAN Dong-yi YUAN Hui-jun YANG Wei-yan Department of otolaryngology Head and Neck Surgery FirstAffiliated Hospital to Chinese General Hospital of PLA Beijing 100037 institute of otolaryngology Chinese General Hospital of PLABeijing 100853 China Department of otolaryngology Head and Neck Surgery CentralHospital Yuncheng 044000 China

Background Waardenburg syndrome type I （WS1） is an autosomal dominant disorder characterized by sensorineural hearing loss, pigmental abnormalities of the eye, hair and skin, and dystopia canthorum. The gene mainly responsible for WS1 is PAX3 which is involved in melanocytic development and survival. Mutations of PAX3 have been reported in familiar or sporadic patients with WS1 in several populations of the world except Chinese. In order to explore the genetic background of Chinese WS1 patients, a mutation screening of PAX3 gene was carried out in four WS1 pedigrees. Methods A questionnaire survey and comprehensive clinical examination were conducted in four Chinese pedigrees of WSI. Genomic DNA from each patient and their family members was extracted and exons of PAX3 were amplified by PCR. PCR fragments were ethanol-purified and sequenced in both directions on an ABl_Prism 3100 DNA sequencer with the BigDye Terminator Cycle Sequencing Ready Reaction Kit. The sequences were obtained and aligned to the wild type sequence of PAX3 with the GeneTool program. Results Two nonsense PAX3 mutations have been found in the study population. One is heterozygous for a novel nonsense mutation S209X. The other is heterozygous for a previously reported mutation in European population R223X. Both mutations create stop codons leading to truncation of the PAX3 protein. Conclusions This is the first demonstration of PAX3 mutations in Chinese WS1 patients and one of the few examples of an identical mutation of PAX3 occurred in different populations.

关键词： Waardenburg syndrome type 1 PAX3 gene mutation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Development of Chinese herbal medicine for sensorineural hearing loss

引用

Acta Pharmaceutica Sinica B 2024年第2期14卷 455-467页

作者： Yunhao Wu Jingwen Zhang Qiuping Liu Zhuang Miao Renjie Chai Wenyong Chen State Key Laboratory of Bioelectronics Department of Otolaryngology Head and Neck SurgeryZhongda HospitalSchool of Life Sciences and TechnologyAdvanced Institute for Life and HealthJiangsu Province HighTech Key Laboratory for Bio-Medical ResearchSoutheast UniversityNanjing 210096China Department of otolaryngology-Head and Neck the Second Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510120China Department of otolaryngology-Head and Neck Surgery Affiliated Hospital of Jiangnan UniversityWuxi 214000China Department of otolaryngology Head and Neck Surgery Sichuan Provincial People’s HospitalUniversity of Electronic Science and Technology of ChinaChengdu 610000China Co-Innovation Center of Neuroregeneration Nantong UniversityNantong 226001China institute for Stem Cell and Regeneration Chinese Academy of ScienceBeijing 100085China Beijing Key Laboratory of Neural Regeneration and Repair Capital Medical UniversityBeijing 100069China

According to the World Health Organization’s world report on hearing,nearly 2.5 billion people worldwide will suffer from hearing loss by 2050,which may contribute to a severe impact on individual life quality and national *** hearing loss(SNHL)occurs commonly as a result of noise exposure,aging,and ototoxic drugs,and is pathologically characterized by the impairment of mechanosensory hair cells of the inner ear,which is mainly triggered by reactive oxygen species accumulation,inflammation,and mitochondrial *** recent advances have been made in understanding the ability of cochlear repair and regeneration,there are still no effective therapeutic drugs for *** herbal medicine which is widely distributed and easily accessible in China has demonstrated a unique curative effect against SNHL with higher safety and lower cost compared with Western *** we present trends in research for Chinese herbal medicine for the treatment of SNHL,and elucidate their molecular mechanisms of action,to pave the way for further research and development of novel effective drugs in this field.

关键词： Sensorineural hearing loss Chinese herbal medicine Molecular mechanism Active ingredients Prescriptions Ototoxic drugs Noise Hair cells

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Yin-Yang and Zheng:Exported from Chinese Medicine

引用

Chinese Journal of Integrative Medicine 2014年第4期20卷 250-255页

作者：张红激王展翔 Department of otolaryngology Indiana University School of Medicine Department of Pediatrics Indiana University School of Medicine

Chinese medicine （CM） basic theory differs substantially from modernmedicine in methodology. CM views the healthy human body as an entity in yin-yang equilibrium. CM practitioners typically diagnose patients with ＂Zheng （CMsyndrome）＂-yin-yang status of specific organs, and the ultimate goal of treatment inCM is to restore the yin-yang balance. This concept might seem strange to Westernperceptions; however, CM philosophy and methodology have been graduallyaccepted and used in the West. There is growing evidence from prestigiousworldwide journals like Nature, Science and Cell indicating that CM theory isbecoming an important component in dealing with health issues in the West.

关键词： Chinese medicine Syndrome basic theory yin-yang Zheng West medicine integration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Image cytometric DNA analysis of mucosal biopsies in patients with primary achalasia

引用

World Journal of Gastroenterology 2006年第19期12卷 3020-3025页

作者： I Gockel P K(a|¨)mmerer J Brieger UR Heinrich WJ Mann F Bittinger VF Eckardt T Junginger Department of General and Abdominal Surgery Johannes Gutenberg-University Mainz Laboratory of Tumor Biology Department of otolaryngology Johannes Gutenberg-UniversityMainz institute of Pathology Johannes Gutenberg-University Mainz Department of Gastroenterology German Diagnostic Clinic Wiesbaden

AIM： To determine DNA aneuploidy in mucosal biopsies of achalasia patients for subsequent rapid diagnosis. METHODS： Biopsies from the middle third of the esophagus were obtained in 15 patients with achalasia. Immunohistochemical staining was carried out with monoclonal antibodies MIB-1 for Ki67 and PAb 1801 for p53, in addition to the conventional histologic examination for dysplasia. Nuclei of fresh biopsy material were enzymatically and mechanically isolated, and the DNA content was determined with image cytometry after Feulgen staining. DNA grading of malignancy was assessed according to Boecking to determine the variability of DNA values noted around the normal diploid peak. Further indices measured included the aneuploid rate, and the 5c-, 7c- and 9c-exceeding rate. RESULTS： The histological examination did not demonstrate dysplasia; while MIB-1 （basal） showed a positive reaction in 8/15 achalasia specimens, p53 was negative in all specimens. Image cytometric DNA analysis detected aneuploidy in 4/15 （26.7%） specimens. Samples from 15 patients with squamous cell carcinoma as well as specimens obtained exclusively 2 cm proximal to the tumor served as reference tests. All carcinomas （15/15） as well as 9 of the peritumoral samples （9/15） were aneuploid. The comparison of biopsies from achalasia patients with peritumoral and carcinoma specimens revealed statistically significant differences regarding the aneuploid rate （diploid： P 〈 0.0001; tetraploid： P = 0.001）, grading of malignancy according to Boecking （P 〈 0.0001） and the 5c- （P 〈 0.0001）, 7c-（P 〈 0.0001）, and 9c- （P = 0.0001） exceeding rate with progredient DNA alterations in the respective order. CONCLUSION： The finding that DNA aneuploidy was identified by image cytometry in esophageal specimens of patients with achalasia, which may be due to specific chromosomal alterations presenting as precancerous lesions in 27% of patients, leads us to conclude that image cytometry represents a valuable screening

关键词： Achalasia Precancerous epithelial alterations Esophageal carcinoma Image cytometric DNA analysis Proliferation marker Ki67 Tumor suppressor gene p53

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Role of the caudate-putamen nucleus in sensory gating in induced tinnitus in rats

引用

Neural Regeneration Research 2021年第11期16卷 2250-2256页

作者： Meng-Lin Wang Yu Song Jun-Xiu Liu Ya-Li Du Shan Xiong Xin Fan Jiang Wang Zhi-Di Zhang Lan-Qun Mao Fu-Rong Ma Department of otolaryngology Head and Neck Surgery Peking University Third HospitalBeijingChina Beijing National Laboratory for Molecular Sciences Key Laboratory of Analytical Chemistry for Living BiosystemsInstitute of Chemistrythe Chinese Academy of SciencesBeijingChina

Tinnitus can be described as the conscious perception of sound without external stimulation,and it is often accompanied by anxiety,depression,and *** clinical treatments for tinnitus are *** recent studies have indicated that the caudateputamen nucleus may be a sensory gating area involved in noise elimination in tinnitus,the underlying mechanisms of this disorder are yet to be *** investigate the potential role of the caudate-putamen nucleus in experimentally induced tinnitus,we created a rat model of tinnitus induced by intraperitoneal administration of 350 mg/kg sodium *** results revealed that the mean spontaneous firing rate of the caudate-putamen nucleus was increased by sodium salicylate treatment,while dopamine levels were *** addition,electrical stimulation of the caudate-putamen nucleus markedly reduced the spontaneous firing rate of neurons in the primary auditory *** findings suggest that the caudate-putamen nucleus plays a sensory gating role in sodium salicylate-induced *** study was approved by the Institutional Animal Care and Use Committee of Peking University Health Science Center(approval No.A2010031)on December 6,2017.

关键词： caudate-putamen nucleus deep brain stimulation dopamine primary auditory cortex sodium salicylate sound striatum tinnitus

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Vasculogenic mimicry is a key prognostic factor for laryngeal squamous cell carcinoma： a new pattern of blood supply

引用

Chinese Medical Journal 2012年第19期125卷 3445-3449页

作者： LIN Peng WANG Wei SUN Bao-cun CAI Wen-juan LI Li LU Hong-hua HAN Chun-rong ZHANG Jin-mei Department of otolaryngology Head and Neck Tianjin First Central Hospital Tianjin Institute of Otolaryngology Tianjin Medical University Tianjin 300192 China Tianjin Medical University Cancer institute and Hospital Tianjin Medical University Tianjin 300060 China

Background Recurrence and local lymph node metastasis affected the prognosis of patients with laryngeal cancer. The aim of this study was to analyze clinical and pathological significance of vasculogenic mimicry （VM） in laryngeal squamous cell carcinoma （LSCC） and evaluate its contribution to prognosis. Methods Data of 168 cases of LSCC were reviewed retrospectively to reveal clinical pathology and prognostic significance of VM. CD31 and periodic acid-Schiff double staining was used to identify VM. Results VM in LSCC contributed to lymph node metastasis （P=0.003） and clinical progression. VM correlated to histopathology grade （P=0.001） of LSCC. VM was an adverse prognostic factor for both disease-specific survival （P=0.039） and metastasis-free survival （P=0.042） by univariate survival analyses. And it was an independent prognostic factor for only disease-specific survival （P=0.003） by multivariate survival analyses. Conclusions VM existed in LSCC. LSCC with VM has more Dotential to invasion and metastasis.

关键词： laryngeal squamous cell carcinoma vasculogenic mimicry prognosis metastasis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：