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Significant expansion and red-shifting of fluorescent protein chromophore determined through computational design and genetic code expansion

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Biophysics Reports 2018年第5期4卷 273-285页

作者： Li Wang Xian Chen Xuzhen Guo Jiasong Li Qi Liu Fuying Kang Xudong Wang Cheng Hu Haiping Liu Weimin Gong Wei Zhuang Xiaohong Liu Jiangyun Wang institute of Biophysics Chinese Academy of Sciences College of Life Sciences University of Chinese Academy of Sciences Key Laboratory of Physics and Technology for Advanced Batteries (Ministry of Education) Department of PhysicsJilin University State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences

Fluorescent proteins(FPs) with emission wavelengths in the far-red and infrared regions of the spectrum provide powerful tools for deep-tissue and super-resolution imaging. The development of red-shifted FPs has evoked widespread interest and continuous engineering efforts. In this article,based on a computational design and genetic code expansion, we report a rational approach to significantly expand and red-shift the chromophore of green fluorescent protein(GFP). We applied computational calculations to predict the excitation and emission wavelengths of a FP chromophore harboring unnatural amino acids(UAA) and identify in silico an appropriate UAA, 2-amino-3-(6-hydroxynaphthalen-2-yl)propanoic acid(naphthol-Ala). Our methodology allowed us to formulate a GFP variant(cpsf GFP-66-Naphthol-Ala) with red-shifted absorbance and emission spectral maxima exceeding 60 and 130 nm, respectively, compared to those of GFP. The GFP chromophore is formed through autocatalytic post-translational modification to generate a planar 4-(p-hydroxybenzylidene)-5-imidazolinone chromophore. We solved the crystal structure of cpsf GFP-66-naphthol-Ala at 1.3 ? resolution and demonstrated the formation of a much larger conjugated p-system when the phenol group is replaced by naphthol. These results explain the significant red-shifting of the excitation and emission spectra of cpsf GFP-66-naphthol-Ala.

关键词： Computational design Green fluorescent protein Red-shift Unnatural amino acids

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Er-Y-Cr-Si化合物在极低温区的巨低场磁热效应

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Science China Materials 2023年第5期66卷 2039-2050页

作者：奚磊郑新奇高亚伟许家旺刘超凡王鼎淞徐菊萍殷雯杨淑娴靳宝杰朱梦媛许玮峯申见昕张静言黄河吴燕飞顾飞史慧宇陶怡璇王守国沈保根 School of Materials Science and Engineering Beijing Advanced Innovation Center for Materials Genome EngineeringUniversity of Science and Technology BeijingBeijing 100083China School of Materials Science and Engineering Anhui UniversityHefei 230601China Beijing National Laboratory for condensed matter Physics Institute of PhysicsChinese Academy of Sciences&University of Chinese Academy of SciencesBeijing 100190China Songshan Lake Materials Laboratory Dongguan 523808China institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China Spallation Neutron Source Science Center Dongguan 523803China Ningbo institute of Materials Technology and Engineering Chinese Academy of SciencesNingbo 315201China

高性能低温低场磁热材料在气体液化等领域具有重要的应用前景.本团队通过真空电弧熔炼的方式成功合成了一系列多晶Er_(1-x)Y_(x)Cr_(2)Si_(2)(0≤x≤0.8)样品,这些材料表现出巨大的低场磁热效应.其中Cr含量为0.1的样品显示出最好的低场... 详细信息

高性能低温低场磁热材料在气体液化等领域具有重要的应用前景.本团队通过真空电弧熔炼的方式成功合成了一系列多晶Er_(1-x)Y_(x)Cr_(2)Si_(2)(0≤x≤0.8)样品,这些材料表现出巨大的低场磁热效应.其中Cr含量为0.1的样品显示出最好的低场磁热性能以及接近2 K的合适的工作温区.更重要的是,在0-1 T的磁场变化下,该样品的最大磁熵变峰值以及最大绝热温变峰值分别高达19.2 J kg^(-1)K^(-1)和4.3 K.其磁熵变峰值为目前已报道的20 K以下温区合金类磁热材料的最大值.通过Arrott曲线,平均场理论以及约化磁熵变曲线等手段,证明了磁相变特征为二级相变.物理机理分析表明,10%的Y替代导致高达15.9%的磁熵变峰值增强的原因在于替代样品所具有的大饱和磁化强度以及小饱和磁场.

关键词： magnetocaloric effect magnetic structure RCr_(2)Si_(2)compounds

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Breaking the concentration quenching limit of lanthanide emitter through multi-coupling of confined quasi-0D & 2D energy migration

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Science China Chemistry 2024年

作者： Yuanchao Lei Shanshan Zhou Jianxi Ke Licheng Yu Youchao Wei Yongsheng Liu Maochun Hong State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences School of Physical Science and Technology Shanghai Tech University Fujian Science & Technology Innovation Laboratory for Optoelectronic Information of China

The widespread applications of lanthanide-doped materials have fuelled a growing demand for precise control over the luminescence characteristics of these materials. However, the limitation imposed by concentration quenching remains a major obstacle in achieving efficient luminescence from lanthanide-doped materials. Herein, a novel strategy utilizing Sc2O3:Ln3+microflowers to enable confined energy migration in both microscopic quasi-zero-dimensional（0D） and mesoscopic twodimensional（2D） multi-coupling systems is proposed to overcome the concentration quenching limit of lanthanide emitters and achieve a remarkable doping amount of 48.14% on a single particle without resorting to complex core/multishell *** studies reveal that the multi-confinement structure effectively restricts the range of energy migration and significantly reduces excitation energy migration to defects. Based on these highly doped Sc2O3microflowers, full spectrum and power-dependent tunable multi-color lanthanide emission in a single particle is successfully achieved. Furthermore, the 2Dencoded patterns derived from these microflowers hold great promise for anti-counterfeiting applications. Our findings emphasize the multi-coupling of confined quasi-0D & 2D energy migration within a well-designed structure, providing valuable insights into concentration quenching mechanisms. This also opens up new opportunities for multi-level anti-counterfeiting systems and information security.

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Effect of number density on velocity distributions in a driven quasi-two-dimensional granular gas

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Chinese Physics B 2010年第10期19卷 617-622页

作者： Sajjad Hussain Shah 李寅阊厚美瑛 institute of Physics Chinese Academy of Sciences Physics Department Beijing Institute of Technology

The motion of mono-disperse spherical steel particles in a vibration driven quasi-two-dimensional （2D） square cell is studied. The cell is horizontally vibrated to eliminate the effect of gravity compaction. The velocity distributions at different particle number densities are studied and found to obey the form exp[-β（｜Vy｜/σy）α], in which Vy and （σy are velocity and its variance in the transverse direction, and α and β are fitting parameters. The value of α is found to decrease with the number density of particles increasing. To investigate the effect of the bottom plate, the molecular dynamics simulation without considering any bottom friction is performed. The accordance between the simulation result and the experimental result shows that the influence of bottom plate friction force on the high energy tail of the velocity distribution can be neglected.

关键词： granular matter velocity distribution

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Modulation of the cutoff wavelength in the spectra for solar selective absorbing coating based on high-entropy films

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International Journal of Minerals,Metallurgy and Materials 2020年第10期27卷 1371-1378页

作者： Ping Song Cong Wang Jie Ren Ying Sun Yong Zhang Angélique Bousquet Thierry Sauvage Eric Tomasella Center for condensed matter and Material Physics Beihang UniversityBeijing 100083China Key Laboratory for Microstructural Material Physics of Hebei Province School of ScienceYanshan UniversityQinhuangdao 066004China Beijing Advanced Innovation Center of Materials Genome Engineering State Key Laboratory for Advanced Metals and MaterialsUniversity of Science and Technology BeijingBeijing 100083China UniversitéClermont Auvergne Centre national de la recherche scientifique(CNRS)SIGMA ClermontInstitut de Chimie de Clermont-Ferrand(ICCF)Clermont-Ferrand F-63000France Extreme Conditions and Materials:High Temperature and Irradiation(CEMHTI-UPR 3079 CNRS Orléans F-45100France

This paper demonstrates an intrinsic modulation of the cutoff wavelength in the spectra for solar selective absorbing coating based on high-entropy ***(NiCuCrFeSi)N((NCCFS)N)films were deposited by a magnetron sputtering *** backscattering spectroscopy analysis confirms the uniform composition and good homogeneity of these high-entropy *** real and imaginary parts of the permittivity for the(NCCFS)N material are calculated on the basis of the reflectance spectral fitting results.A redshift cutoff wavelength of the reflectance spectrum with increasing nitrogen gas flow rate exists because of the different levels of dispersion when changing nitrogen *** realize significant solar absorption,the film surface was reconstituted to match its impedance with air by designing a pyramid nanostructure *** with the absorptance of the as-deposited films,the designed metasurface obtains a significant improvement in solar absorption with the absorptance increasing from 0.74 to *** metasurfaces also show low mid-infrared emissions with thermal emittance that can be as low as *** results demonstrate a new idea in the design of solar selective absorbing surface with controllable absorptance and low infrared emission for high-efficiency photo-thermal conversion.

关键词： cutoff wavelength solar selective absorption infrared emission nanostructure high-entropy film

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Cooperative control of perpendicular magnetic anisotropy via crystal structure and orientation in freestanding SrRuO_(3)membranes

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npj Flexible Electronics 2022年第1期6卷 83-90页

作者： Zengxing Lu Yongjie Yang Lijie Wen Jiatai Feng Bin Lao Xuan Zheng Sheng Li Kenan Zhao Bingshan Cao Zeliang Ren Dongsheng Song Haifeng Du Yuanyuan Guo Zhicheng Zhong Xianfeng Hao Zhiming Wang Run-Wei Li CAS Key Laboratory of Magnetic Materials and Devices Ningbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingbo 315201China Zhejiang Province Key Laboratory of Magnetic Materials and Application Technology Ningbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingbo 315201China School of Materials and Metallurgy University of Science and Technology LiaoningAnshan 114051China Key Laboratory of Applied Chemistry College of Environmental and Chemical EngineeringYanshan UniversityQinhuangdao 066004China University of Nottingham Ningbo 315201China Nano Science and Technology institute University of Science and Technology of ChinaHefei 230026China institutes of Physical Science and Information Technology Anhui UniversityHefei 230601China Anhui Key Laboratory of condensed matter Physics at Extreme Conditions High Magnetic Field LaboratoryHFIPSAnhuiChinese Academy of SciencesHefei 230031China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China

Flexible magnetic materials with robust and controllable perpendicular magnetic anisotropy(PMA)are highly desirable for developing flexible high-performance spintronic ***,it is still challenge to fabricate PMA films on polymers ***,we report a facile method for synthesizing single-crystal freestanding SrRuO_(3) membranes with controlled crystal structure and orientation using water-soluble Ca_(3-x)Sr_(x)Al_(2)O_(3)6 sacrificial *** cooperative effect of crystal structure and orientation,flexible membranes reveal highly tunable magnetic anisotropy from in-plane to out-of-plane with a remarkable PMA energy of 7×10^(6) erg·cm^(−3).First-principle calculations reveal that the underlying mechanism of PMA modulation is intimately correlated with structure-controlled Ru 4d-orbital occupation,as well as spin-orbital matrix element differences,dependent on the crystal *** addition,even after 10,000 bending cycles,the PMA keeps stable,indicating a robust magnetism reliability in the prepared *** work provides a feasible approach to prepare the flexible oxide films with strong and controllable PMA.

关键词： anisotropy magnetic structure

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双J/ψ质量谱中的第三个峰结构

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Science Bulletin 2023年第8期68卷 800-803,M0003页

作者：牛鹏宇张振宇王倩杜孟林 Guangdong Provincial Key Laboratory of Nuclear Science Institute of Quantum MatterSouth China Normal UniversityGuangzhou 510006China Guangdong-Hong Kong Joint Laboratory of Quantum matter Southern Nuclear Science Computing CenterSouth China Normal UniversityGuangzhou 510006China School of Physics University of Electronic Science and Technology of ChinaChengdu 611731China

强相互作用是自然界中四种基本相互作用之一,其色禁闭性质决定实验上能直接观测到的粒子都是色单态的强子.因此,研究强子的结构和性质是理解强子化的最重要的途径之一.近些年,国内外各大高能实验发现了大量超出传统夸克模型的奇特强子... 详细信息

强相互作用是自然界中四种基本相互作用之一,其色禁闭性质决定实验上能直接观测到的粒子都是色单态的强子.因此,研究强子的结构和性质是理解强子化的最重要的途径之一.近些年,国内外各大高能实验发现了大量超出传统夸克模型的奇特强子态候选者,这为研究强子形成的动力学机制提供了独特的环境.2020年欧洲核子中心的LHCb合作组在J/ψJ/ψ不变质量谱中发现两个峰值结构.两年后,CMS和ATLAS合作组分别在J/ψJ/ψ和J/ψψ’不变质量谱中7.2 GeV附近发现第三个峰结构.本文研究表明ηc(2S)ηc(2S)对第三个峰结构至关重要.由于散射矩阵的幺正性,J/ψJ/ψ不变质量谱中7.2 GeV处的峰结构与同能量处不变质量谱中的低谷结构同时存在.文章建议实验在7.2 GeV能量处同时细致扫描J/ψJ/ψ和J/ψψ’的不变量质量谱.这为确定全粲四夸克态的性质提供了独特的线索.

关键词：强相互作用夸克模型强子散射矩阵动力学机制候选者质量谱基本相互作用

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Highly Dispersive Metal Atoms Anchored on Carbon Matrix Obtained by Direct Rapid Pyrolysis of Metal Complexes

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CCS Chemistry 2022年第9期4卷 2968-2979页

作者： Bing Huang Minghao Wang Chuxin Wu Lunhui Guan CAS Key Laboratory of Design and Assembly of Functional Nanostructures Fujian Key Laboratory of NanomaterialsFujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou 350108 University of Chinese Academy of Sciences Beijing 100049 Fujian Normal University Fuzhou 350108

The oxygen reduction reaction(ORR)and oxygen evolution reaction(OER)are critical in energy conversion and storage ***-atom catalysts(SAC)with atomic utilization efficiency are among the most promising candidates for oxygen *** on the controllable and efficient synthesis of SACs,we develop a direct rapid pyrolysismethod that shortens the synthesis process from several hours to 1 min.

关键词： electrochemical catalysis rapid pyrolysis carbon materials bifunctional catalysts zinc–air batteries

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Nonpolar Na_(10)Cd(NO_(3))_(4)(SO_(3)S)_(4) Exhibits a Large Second-Harmonic Generation

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CCS Chemistry 2022年第2期4卷 526-531页

作者： Youchao Liu Youquan Liu Zheshuai Lin Yanqiang Li Qingran Ding Xin Chen Lina Li Sangen Zhao Maochun Hong Junhua Luo State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhouFujian 350002 Technical institute of Physics and Chemistry Chinese Academy of SciencesBeijing 100190 University of the Chinese Academy of Sciences Beijing 100049 Fujian Science&Technology Innovation Laboratory for Optoelectronic Information of China FuzhouFujian 350108

Conventional wisdom says that nonpolar structures do not favorably produce strong second-harmonic generation(SHG)responses since the polarization in their microscopic functional groups are ***,we report the first nonlinear optical thiosulfate,Na_(10)Cd(NO_(3))_(4)(SO_(3)S)_(4),which crystallizes in a nonpolar space group of P−***,this thiosulfate exhibits a strong SHG response of about 4.2 times that of the benchmark KH2PO4,which is larger than those of polar *** to first-principles calculations and a flexible dipole model,the SHG enhancement is mainly ascribed to the larger flexibility of the S=S bond in the SO_(3)S tetrahedra in comparison with the S–O bond in the SO4 *** findings indicate that constructing flexible bonds is an effective strategy to design highperformance nonlinear optical materials regardless of polarity.

关键词： nonlinear optical material secondharmonic generation crystal structure structure-property relationships thiosulfate

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Boosting photocatalytic hydrogen evolution of g-C_(3)N_(4) catalyst via lowering the Fermi level of co-catalyst

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Nano Research 2022年第2期15卷 1128-1134页

作者： Hairui Cai Bin Wang Laifei Xiong Jinglei Bi Hanjing Hao Xiaojing Yu Chao Li Jiamei Liu Shengchun Yang MOE Key Laboratory for Non-equilibrium Synthesis and Modulation of condensed matter Key Laboratory of Shaanxi for Advanced Materials and Mesoscopic PhysicsState Key Laboratory for Mechanical Behavior of MaterialsSchool of PhysicsXian Jiaotong UniversityNo.28 West Xianning RoadXi'an 710049China Shaanxi Collaborative Innovation Center for Hydrogen Fuel Cell Performance Improvement Xian Jiaotong UniversityNo.28 West Xianning RoadXi'an 710049China School of Materials Science and Engineering Xi'an University of TechnologyXi'an 710048China Instrument Analysis Center of Xian Jiaotong University Xian Jiaotong UniversityNo.28 West Xianning RoadXi'an 710049China

The photocatalytic performances are highly dependent on the charge separation and surface reaction kinetics of *** at figuring out the effects of co-catalyst with the lower Fermi level on photocatalytic activity,we tuned the Fermi level of Pt nanoparticles on g-C_(3)N_(4)(GCN)by introducing Co *** results show that lowering the Fermi level of co-catalyst does not alter light absorption of GCN due to the invariable ***,Pt_(3)Co with a lower Fermi level contributes less positive influence on charge separation in GCN due to an opposite effect from the stronger electron-trap ability of Pt_(3)Co and increased band bending in GCN-Pt_(3)*** density functional theory(DFT)calculations indicate that GCN-Pt_(3)Co has faster surface reaction kinetics than GCN-Pt,owing to easier dissociation of H_(2)O molecules and faster desorption of H^(*)on Pt_(3)***,GCN-Pt_(3)Co exhibits an excellent H_(2) evolution rate with 2.91 mmol g^(-1)·h^(-1),which 2.67 times that of GCN-Pt.

关键词： Schottky heterojunction surface reactions band bending Fermi level photocatalytic hydrogen evolution

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