检索结果-南通市图书馆

Enrichment of ER tubule-derived microsomes by differential centrifugation and immunoprecipitation

在线全文

学校读者我要写书评

暂无评论

biophysics Reports 2024年第2期10卷 61-66页

作者： Yiduo Liu Junjie Hu Bing Yan National Laboratory of Biomacromolecules CAS Center for Excellence in Biomacromolecules Institute of Biophysics Chinese Academy of Sciences University of Chinese academy of sciences

The endoplasmic reticulum(ER) is an essential component of the endomembrane system in eukaryotes and plays a crucial role in protein and lipid synthesis, as well as the maintenance of calcium homeostasis. Morphologically, the ER is composed primarily of sheets and tubules. The tubular ER is composed of a network of tubular membrane structures, each with diameters ranging from 30 to 50nanometers. In recent years, there has been in-depth research on the molecular mechanisms of membrane shaping and membrane fusion of the tubular ER. However, there is still limited understanding of the specific physiological functions of the tubular ER. Here, we report a protocol that combines differential centrifugation and immunoprecipitation to specifically enrich microsomes originating from the tubular ER in yeast. The ER tubule-derived microsomes can be further used for proteomic and lipidomic studies or other biochemical analyses.

关键词： Endoplasmic reticulum Microsome Differential centrifugation Immunoprecipitation

Complicated target recognition by archaeal box C/D guide RNAs

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science China(Life sciences) 2024年第4期67卷 631-644页

作者： Jiayin Wang Songlin Wu Keqiong Ye Key Laboratory of RNA Science and Engineering CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China University of Chinese academy of sciences Beijing 100049China

Box C/D RNAs guide the site-specific formation of 2′-O-methylated nucleotides(Nm)of RNAs in eukaryotes and *** C/D RNAs have been profiled in several archaea,their targets have not been experimentally ***,we mapped Nm in r RNAs,t RNAs,and abundant small RNAs(s RNAs)and profiled C/D RNAs in the crenarchaeon Sulfolobus *** targets of C/D RNAs were assigned by analysis of base-pairing interactions,in vitro modification assays,and gene deletion experiments,revealing a complicated landscape of C/D RNA-target interactions.C/D RNAs widely use dual antisense elements to target adjacent sites in r RNAs,enhancing modification at weakly bound *** consecutive sites can be guided with the same antisense element upstream of box D or D′,a phenomenon known as doublespecificity that is exclusive to internal box D′in eukaryotic C/D *** C/D RNAs guide modification at a single non-canonical *** study reveals the global landscape of RNA-guided 2′-O-methylation in an archaeon and unexpected targeting rules employed by C/D RNA.

关键词： C/D RNA 2′-O-methylation archaea guide RNA RNA modification

FL-Online:An x-ray crystallographic web-server for atomic-scale structure analysis of biomolecule

在线全文

学校读者我要写书评

暂无评论

Chinese Physics B 2024年第7期33卷 37-42页

作者：王宾堂牛彤欣范海福丁玮 Beijing National Laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190China University of Chinese academy of sciences Beijing 100049China Songshan Lake Materials Laboratory Dongguan 523808China Center for Biological Imaging Core Facilities for Protein ScienceInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China

FL-Online(http://***) is an out-of-box modern web service featuring a user-friendly interface and simplified parameters, providing academic users with access to a series of online programs for biomolecular crystallography, including SAPI-online, OASIS-online, C-IPCAS-online and a series of upcoming software releases. Meanwhile, it is a highly scalable and maintainable web application framework that provides a powerful and flexible solution for academic web development needs. All the codes are open-source under MIT licenses in GitHub.

关键词： graphical web service OASIS IPCAS direct methods protein crystallography

Numerical evaluations on the fluid production in the in-situ conversion of continental shale oil reservoirs

在线全文

学校读者我要写书评

暂无评论

Petroleum Science 2024年第4期21卷 2485-2501页

作者： Zhao-Bin Zhang Maryelin Josefina Brice?o Montilla Shou-Ding Li Xiao Li Jian-Peng Xing Yan-Zhi Hu Key Laboratory of Shale Gas and Geoengineering Institute of Geology and Geophysics Chinese Academy of Sciences institute of Earth sciences Chinese Academy of Sciences College of Earth and Planetary sciences University of Chinese Academy of Sciences institute of Energy Peking University

In-situ conversion presents a promising technique for exploiting continental oil shale formations,characterized by highly fractured organic-rich rock. A 3D in-situ conversion model, which incorporates a discrete fracture network, is developed using a self-developed thermal-flow-chemical(TFC) *** of the model elucidates the in-situ conversion process in three stages and defines the transformation of fluids into three distinct outcomes according to their end stages. The findings indicate that kerogen decomposition increases fluid pressure, activating fractures and subsequently enhancing permeability. A comprehensive analysis of activated fracture permeability and heating power reveals four distinct production modes, highlighting that increasing heating power correlates with higher cumulative fluid production. Activated fractures, with heightened permeability, facilitate the mobility of heavy oil toward production wells but hinder its cracking, thereby limiting light hydrocarbon ***, energy efficiency research demonstrates the feasibility of the in-situ conversion in terms of energy utilization, especially when considering the surplus energy from high-fluctuation energy sources such as wind and solar power to provide heating.

关键词： In-situ conversion Continental shale oil Natural fracture network TFC model

Development of Ti–V–Cr–Mn–Mo–Ce high-entropy alloys for high-density hydrogen storage in water bath environments

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Rare Metals 2024年第10期43卷 5229-5241页

作者： Hua-Zhou Hu Hou-Qun Xiao Xin-Cong He Wen-Hao Zhou Xiao-Xuan Zhang Rui-Zhu Tang Jie Li Chuan-Ming Ma Qing-Jun Chen Key Laboratory of Rare Earths Chinese Academy of SciencesJiangxi Institute of Rare EarthsGanjiang Innovation AcademyChinese Academy of Sciences

The V-based body-centered cubic （BCC）-type hydrogen storage alloys have attracted significant attention due to their high theoretical hydrogen storage capacity of3.80 wt%.However,their practical application faces challenges related to low dehydriding capacity and poor activation *** overcome these challenges,a BCC-type Ti–V–Cr–Mn–Mo–Ce high-entropy alloy（HEA） with an effectively dehydriding capacity of 2.5 wt% above 0.1 MPa was *** introduction of Mo and conducting heat treatment,the precipitation of Ti-rich phase in HEA was successfully suppressed,resulting in improved compositional uniformity and dehydriding ***,the effective dehydriding capacity increased significantly from 0.60 wt% to 2.50 wt% a65℃,surpassing that of other types of hydrogen storage alloys under the same ***,the addition of 1 wt%Ce enabled initial hydrogen absorption at 25℃ without the need for activation at 400℃.Furthermore,Ce doping reduced the dehydriding activation energy of the Ti–V–Cr–Mn–Mo–Ce HEA from 52.71 to 42.82 kJ·mol-1Additionally,the enthalpy value of dehydrogenation decreased from 46.89 to 17.96 k J·mol-1,attributed to a decrease in the hysteresis factor from 0.68 to *** findings provide valuable insights for optimizing the hydrogen storage property of HEA.

关键词： BCC structure Ce doping Heat treatment High-entropy alloys Hydrogen storage alloys

Pseudoeffective thresholds and cohomology of twisted symmetric tensor fields on irreducible Hermitian symmetric spaces

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science China Mathematics 2024年第11期67卷 2433-2452页

作者： Feng Shao institute of Mathematics Academy of Mathematics and Systems ScienceChinese Academy of Sciences School of Mathematical sciences University of Chinese Academy of Sciences

Let X be an irreducible Hermitian symmetric space of compact type(IHSS for short).In this paper,we give the irreducible decomposition of *** a by-product,we give a cohomological characterization of the rank of ***,we introduce pseudoeffective thresholds to measure the bigness of tangent bundles of smooth complex projective varieties precisely and calculate them for irreducible Hermitian symmetric spaces of compact type.

关键词： tangent bundle pseudoeffective threshold irreducible Hermitian symmetric space of compact type (IHSS) multiplicity free action

SARS-CoV-2 ORF8 does not function in the nucleus as a histone mimic

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Protein & Cell 2024年第2期15卷 79-82页

作者： Ping Liu Junjie Hui Lei Wang National Laboratory of Biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing100101China College of Life sciences University of Chinese Academy of SciencesBeijing 100049China

Disruption of epigenetic regulation in host cells is a common evasion strategy used by viruses(Ozturkler and Kalkan 2021).Recently,Kee et al.(2022)reported that accessory protein ORF8 of SARS-CoV-2 can diffuse into the cell nucleus and mimic histone H3 through the ARKS motif,thereby interfering with H3 post-translational modifications(PTMs),disrupting gene transcription,and diminishing the antiviral response.

关键词： mimic thereby function

Recursive Projected Filter Algorithm with Binary-Valued Observations

在线全文

学校读者我要写书评

暂无评论

Journal of Systems Science & Complexity 2024年第5期37卷 1832-1860页

作者： ZHANG Xiaoyan WANG Ying XUE Wenchao ZHAO Yanlong Key Laboratory of Systems and Control Institute of Systems Science Academy of Mathematics and Systems Science Chinese Academy of Sciences School of Mathematical sciences University of Chinese Academy of Sciences

This paper focuses on the state estimate for a class of systems with both process noise and measurement noise under binary-valued observations, in which the Gaussian assumption on the predicted density of the state is not required. A recursive projected filter algorithm with time-varying thresholds is constructed to estimate the state under binary-valued observations. The time-varying thresholds are designed as the prediction value of the measurement, which can provide more information about the system state. The convergence property is established with some suitable stability,boundedness and observability conditions. In particular, the estimation error between state and estimate is proved to be asymptotically bounded in the mean-square sense, whose upper bound is related to the variance of process noise. Finally, the theoretical results are demonstrated via numerical examples of first-order and high-order systems.

关键词： Binary-valued observations convergence recursive filter time-varying thresholds

How to build a habitable planet:an interview with Charles H. Langmuir

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

National Science Review 2024年第6期11卷 76-80页

作者： Yi-Gang Xu the Guangzhou institute of Geochemistry Chinese Academy of Sciences

Earth is the only known habitable planet in the solar system. Understanding how Earth developed its unique habitability has been the frontier of Earth sciences and has become one of the main themes of current deep-space explorations. What are the decisive factors that led to a habitable planet? What is the role of solid Earth processes in the origin of life and in modulating the surface environment? Are Earth’s habitability studies relevant to current challenges that human beings face? These questions have attracted the interest of both scientists and the public alike.

关键词： Earth (planet)