检索结果-南通市图书馆

Molecular Plant 2009年第6期2卷 1247-1261页

作者： Andrea Brautigam Andreas RM. Weber institut fur biochemie der pflanzen Heinrich Heine Universitat Universitatsstrasse 1 D-40225 Dusseldorf Germany Genetics Program Michigan State University East Lansing MI USA Department of Plant Biology Michigan State University East Lansing MI USA

Proplastids are undifferentiated plastids of meristematic tissues that synthesize amino acids for protein synthesis, fatty acids for membrane lipid production, and purines and pyrimidines for DNA and RNA synthesis. Unlike chloroplasts, proplastids depend on supply, with reducing power, energy, and precursor metabolites from the remainder of the cell. Comparing proplastid and chloroplast envelope proteomes and the corresponding transcriptomes of leaves and shoot apex revealed a clearly distinct composition of the proplastid envelope. It is geared towards import of metabolic precursors and export of product metabolites for the rapidly dividing cell. The analysis also suggested a new role for the triosephosphate translocator in meristematic tissues, identified the route of organic nitrogen import into proplastids, and detected an adenine nucleotide exporter. The protein import complex contains the import receptors Toc120 and Toc132 and lacks the redox sensing complex subunits of Tic32, Tic55, and Tic62, which mirrors the expression patterns of the corresponding genes in leaves and the shoot apex. We further show that the protein composition of the internal membrane system is similar to etioplasts, as it is dominated by the ATP synthase complex and thus remarkably differs from that of chloroplast thylakoids.

关键词： Membrane biochemistry proteomics transporters chloroplast biology membrane proteins transcriptome analysis.

Immunogenic efficacy of differently produced recombinant vaccines candidates against Pseudomonas aeruginosa infections

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

第二军医大学学报 1998年第S1期19卷 98-103页

作者： Von Specht BU Gabelsberger J△ Knapp B▲ Hundt E▲ SchmidtPilger H Bauernsachs S△Lenz U▲ Domdey H△ From Chirurgische Universitatsklinik der Universitat Freiburg Freiburg Germany institut fur biochemie-Genzentrum-der LMU Munchen Munchen Germany Chiron Behring GmbH Marberg Germany

Ｐｓｅｕｄｏｍｏｎａｓａｅｒｕｇｉｎｏｓａｉｓａｃｏｍｍｏｎｃａｕｓｅｏｆｉｌｎｅｓｓａｎｄｄｅａｔｈｉｎｉｍｍｕｎｏｃｏｍｐｒｏｍｉｓｅｄｐａｔｉｅｎｔｓ．Ｉｎｐａｒｔｉｃｕｌａｒ，ｔｈｅｒｉｓｋｔｏｔｈｏｓｅｓｕ... 详细信息

Ｐｓｅｕｄｏｍｏｎａｓａｅｒｕｇｉｎｏｓａｉｓａｃｏｍｍｏｎｃａｕｓｅｏｆｉｌｎｅｓｓａｎｄｄｅａｔｈｉｎｉｍｍｕｎｏｃｏｍｐｒｏｍｉｓｅｄｐａｔｉｅｎｔｓ．Ｉｎｐａｒｔｉｃｕｌａｒ，ｔｈｅｒｉｓｋｔｏｔｈｏｓｅｓｕｆｅｒｉｎｇｆｒｏｍｓｅ－ｖｅｒｅ...

关键词： Pseudomonas aeruginosa outer membrane protein vaccine

Neuronspezifische　Enolase　（NSE）：　ein　Serummarker　der　klinischen　Progression　für　maligne　metastasierte　Melanome

在线全文

学校读者我要写书评

暂无评论

Journal of Huazhong University of Science and Technology(Medical Sciences) 1995年第1期15卷 19-25页

作者：郭华北 Abteilung für Klinische biochemie Bengbu Medizinische Institut Bengbu institut für Klinische biochemie der Universität Bonn Deutschland

Die Serumkonzentration der neuronspezifischen Enolase(NSE)wurde bei 126 Patienten mit malignem Melanom(MM,Stadium Ⅰ/Ⅱ:n=80;Stadium Ⅲ:n=23;Stadium Ⅳ:n=23)radioimmunologisch *** NSEKonzentrationen(Schwellenwert:>12,5μg/L)fanden sich bei 12,5% der Patienten mit MM im Stadium Ⅰ/Ⅱ,13,0% im Stadium Ⅲ und bei 39,1% im Stadium Ⅳ.In der Langzeit-Verlaufskontrolle bei 20 Patienten im Stadium Ⅲ/Ⅳ zeigte die NSESerumkonzentration eine Ubereinstimmung mit der *** 6 Patienten zeigte sich unter der Therapie ein Abfall erhohter NSE-Werte als Zeichen einer *** Ausbleiben eines signifikanten Abfalls oder gar ein Asstieg wies in 86,7%(13/15)der Falle auf eine Tumorprogression *** signifikanter Anstieg von NSE wurde bei 6 Patienten prdfinal *** laβt sich folgern,daβ NSE fur die Primardiagnostik des malignen Melanoms aufgrund der niedrigen Sensitivitatat als Tumormarker ungeeignet ist;jedoch erscheint eine NSE-Bestimmung bei Patienten mit malignem Melanom im Rahmen einer Verlaufskontrolle sinnvoll.

关键词： Malignes Melanom NSE Serum-Tumormarker

Heat Shock Factors HsfB 1 and HsfB2b Are Involved in the Regulation of Pdf1.2 Expression and Pathogen Resistance in Arabidopsis

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2009年第1期2卷 152-165页

作者： Mukesh Kumar Wolfgang Busch Hannah Birke Birgit Kemmerling Thorsten Nurnberger Friedrich Schoffl Present address: Heinrich-Pette-lnstitut Martinistrabe 52 D-20251 Hamburg Germany Max-Planck-lnstitut fur Entwicklungsbiologie Abteilung Molekularbiologie Speemannstrabe 37-39 D-72076 Tubingen Germany Universitat Tubingen Zentrum for Molekularbiologie der Pflanzen (ZMBP)--AIIgemeine Genetik Auf der Morgenstelle 28 D-72076 Tubingen Germany Universitat Tubingen Zentrum fur Molekularbiologie der Pflanzen (ZMBP)--Biochemie der Pflanzen Auf der Morgenstelle 5 D-72076 Tubingen Germany

In order to assess the functional roles of heat stress-induced class B-heat shock factors in Arabidopsis, we investigated T-DNA knockout mutants of AtHsfB1 and AtHsfB2b. Micorarray analysis of double knockout hsfB1/hsfB2b plants revealed as strong an up-regulation of the basal mRNA-levels of the defensin genes Pdfl.2a/b in mutant plants. The Pdfexpression was further enhanced by jasmonic acid treatment or infection with the necrotrophic fungus Alternaria brassicicola. The single mutant hsfB2b and the double mutant hsfB 1/B2b were significantly improved in disease resistance after A. brassicicola infection. There was no indication for a direct interaction of Hsf with the promoter of Pdf1.2, which is devoid of perfect HSE consensus Hsf-binding sequences. However, changes in the formation of late HsfA2-dependent HSE binding were detected in hsfB1/B2b plants. This suggests that HsfB1/B2b may interact with class A-Hsf in regulating the shut-off of the heat shock response. The identification of Pdfgenes as targets of Hsf-dependent negative regulation is the first evidence for an interconnection of Hsf in the regulation of biotic and abiotic responses.

关键词： Abiotic/environmental stress transcriptional control and transcription factors transcriptome analysis defense responses disease resistance Arabidopsis.

The Arabidopsis Protein CGLD11 Is Required for Chloroplast ATP Synthase Accumulation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2016年第6期9卷 885-899页

作者： Sabine Grahl Bennet Reiter Irene Luise Gugel Evgenia Vamvaka Chiara Gandini Peter Jahns Jurgen Soil Dario Leister Thilo Ruhle Lehrstuhl fur biochemie und Physiologie der pflanzen (Botanik) Department Biologie I Ludwig-Maximilians-Universitat Munchen 82152 Planegg-MartinsriedGermany Lehrstuhl for Molekularbiologie der pflanzen (Botanik) Department Biologie I Ludwig-MaximHians-Universitat MQnchen 82152 Planegg-Martinsried Germany Plant Biochemistry Heinrich-Heine-University DLisseldorf Universittsstrasse 1 40225 Desseldorf Germany Munich Centre for Integrated Protein Science CiPSM Ludwig-Maximilians Universitat Menchen Butenandtstr. 5 - 13 81377 Munich Germany These authors contributed equally to this article.

ATP synthases in chloroplasts （cpATPase） and mitochondria （mtATPase） are responsible for ATP production during photosynthesis and oxidative phosphorylation, respectively. Both enzymes consist of two multi- subunit complexes, the membrane-bound coupling factor O and the soluble coupling factor 1. During cpATPase biosynthesis, several accessory factors facilitate subunit production and orchestrate complex assembly. Here, we describe a new auxiliary protein in Arabidopsis thaliana, which is required for cpATPase accumulation. AtCGLD11 （CONSERVED IN THE GREEN LINEAGE AND DIATOMS 11） is a protein without any known functional domain and shows dual localization to chloroplasts and mitochondria. Loss of AtCGLDll function results in reduced levels of cpATPase and impaired photosynthetic performance with lower rates of ATP synthesis. In yeast two-hybrid experiments, AtCGLD11 interacts with the 13 subunits of the cpATPase and mtATPase. Our results suggest that AtCGLD11 functions in F1 assembly during cpATPase biogenesis, while its role in mtATPase biosynthesis may not, or not yet, be essential.

关键词： chloroplast mitochondria photosynthesis dual targeting ATP synthase assembly

Chapter model systems in heterogeneous catalysis at the atomic level: a personal view

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Chemistry 2020年第4期63卷 426-447页

作者： Hans-Joachim Freund Markus Heyde Helmut Kuhlenbeck Niklas Nilius Thomas Risse Thomas Schmidt Shamil Shaikhutdinov Martin Sterrer Fritz Haber institute of the Max Planck Society Faradayweg 4-6Berlin D-14195Germany Fakultat V-Mathematik und Naturwissenschaften Carl von Ossietzky Universitat OldenburgGermany institut fur Chemie und biochemie Freie UniversitatBerlinGermany institute of Physics University of GrazGrazAustria

The review presents an overview of studies in the surface science of oxide and related surfaces with an emphasis of the studies performed in the authors’ group. Novel instruments and technique developments, as well as their applications are reported, in an attempt to cover studies on model systems of increasing complexity, including some of the key ingredients of an industrially applied heterogeneous catalyst and its fabrication. The review is intended to demonstrate the power of model studies in understanding heterogeneous catalysis at the atomic level. The studies include those on supported nano-particles, both, prepared in vacuum and from solution, interaction of surfaces and the underlying bulk with molecules from the gas phase, strong metal support interaction, as well as the first attempt to include studies on reactions in confined spaces.

关键词： model catalysts oxide surfaces structure and spectroscopy supported nanoparticles reactions in the confined space

Transgenic Poplar Plants for the Investigation of ABA-Dependent Salt and Drought Stress Adaptation in Trees

在线全文

学校读者我要写书评

暂无评论

American Journal of Plant Sciences 2016年第9期7卷 1337-1356页

作者： Domenica Hamisch David Kaufholdt Jennifer-Christin Kuchernig Florian Bittner Ralf R. Mendel Robert Hänsch Jennifer Popko institut für pflanzenbiologie Technische Universitat Braunschweig Braunschweig Germany Albrecht-von-Haller-institutfür pflanzenwissenschaften Abteilung Biochemie der Pflanzen Georg-August-Universitat Gottingen Germany

Important functions of the plant hormone abscisic acid (ABA) in stress reactions, growth and photosynthetic processes are extensively studied in the model plant Arabidopsis thaliana. This paper investigates the importance of Moco-sulphurase ABA3 and aldehyde oxidase (AO) on ABA-biosynthesis in Populus × canescens. ABA3 is essential for activation of the molybdenum enzymes AO and xanthine dehydrogenase (XDH). AO itself catalyzes the last step in ABA-biosynthesis. Generation of transgenic poplar plants altered in ABA3 and AO-activity using RNAi knock down and overexpression was performed. Whereas RNAi-AO plants show a specific loss of AO activity, the RNAi-ABA3 plants has a strongly reduced activity of both molybdenum enzymes: AO and XDH. Constructs of AO and ABA3-promoters fused to β-glucuronidase provide the basis to investigate transcriptional regulation of ABA-biosynthetic processes under stress conditions. Application of high salt concentrations and different drought stress intensities does change the endogenous AO or XDH neither on the side of transcription nor on protein activity. On phytohormone level however, water loss leads to increased ABA-amounts regardless of whether transgenic or wildtype plants are studied. Salt application resulted in higher ABA-levels in all analyzed plant lines. The down regulation of AO in the two different RNAi-plant lines strongly prevented a wildtype-like increase of ABA-levels. Whereas the WT plants accumulated up to 6000 ng ABA g-1 FW-1 after 16 h of salt stress exposure, plants of the RNAi lines revealed a markedly lower increase of only up to 2000 ng ABA g-1 FW-1. Opposing to these observations, ABA-levels increased during drought without any influence by the RNAi-effect. These results revealed that although stresses did not result in a visible increased AO-activity, ABA-production was influenced by AO and ABA3 at least under salinity.

关键词： ABA Biosynthesis Drought Stress Populus × canescens Transgenic Poplar Salt Stress

Ca^(2+)-dependent successive phosphorylation of vacuolar transporter MTP8 by CBL2/3-CIPK3/9/26 and CPK5 is critical for manganese homeostasis in Arabidopsis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2022年第3期15卷 419-437页

作者： Chuanfeng Ju Zhenqian Zhang Jinping Deng Cuicui Miao Zhangqing Wang Lukas Wallrad Laiba Javed Dali Fu Ting Zhang Jörg Kudla Zhizhong Gong Cun Wang State Key Laboratory of Crop Stress Biology for Arid Areas College of Life Sciencesand Institute of Future AgricultureNorthwest Agriculture&Forestry UniversityYanglingShaanxi 712100China State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China institut fur Biologie und Biotechnologie der pflanzen Westfälische Wilhelms-Universität48149 MünsterGermany College of Life Sciences Hebei UniversityBaodingChina

Manganese(Mn)is an essential micronutrient for all living ***,excess Mn supply that can occur in acid or waterlogged soils has toxic effects on plant physiology and *** a variety of Mn transporter families have been characterized,we have only a rudimentary understanding of how these transporters are regulated to uphold and adjust Mn homeostasis in ***,we demonstrate that two calcineurin-B-like proteins,CBL2/3,and their interacting kinases,CIPK3/9/26,are key regulators of plant Mn *** mutants lacking CBL2 and 3 or their interacting protein kinases CIPK3/9/26 exhibit remarkably high Mn ***,CIPK3/9/26 interact with and phosphorylate the tonoplast-localized Mn and iron(Fe)transporter MTP8 primarily at Ser35,which is conserved among MTP8 proteins from various *** transport complementation assays in yeast combined with multiple physiological assays indicate that CBL-CIPK-mediated phosphorylation of MTP8 negatively regulates its transport activity from the cytoplasm to the ***,we show that sequential phosphorylation of MTP8,initially at Ser31/32 by the calcium-dependent protein kinase CPK5 and subsequently at Ser35 by CIPK26,provides an activation/deactivation fine-tuning mechanism for differential regulation of Mn ***,our findings define a two-tiered calcium-controlled mechanism for dynamic regulation of Mn homeostasis under conditions of fluctuating Mn supply.

关键词： manganese Ca^(2+) calcineurin B-like proteins(CBLs) CBL-interacting protein kinases(CIPKs) Mn transporter MTP8 calcium-dependent protein kinases(CPKs)

Plants as concept generators for biomimetic light-weight structures with variable stiffness and self-repair mechanisms

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Bionic Engineering 2004年第4期1卷 199-205页

作者： Thomas Speck Tom Masselter Bettina Prim Olga Speck Rolf Luchsinger Siegfried Fink KompetenznetzBiomimetik der Universit tFreiburg Sch nzlestr.1 D179104 Freiburg Germany Botanischer Garten der Universit t Freiburg Sch nzlestr.1D179104 FreiburgGermany Prospective conceptsag Flughofstr.41 CH18152 Glattbrugg Switzerland institut fur Forsfbotanik und Baumphysiologie der UniversittFreiburg Sch nzlest:1D179104 FreiburgGemmany

Plants possess many structural and functional properties that have a high potential to serve as concept generators for the production of biomimetic technical materials and structures. We present data on two features of plants (variable stiffness due to pressure changes in cellular structures and rapid self-repair functions) that may be used as models for biomimetic projects.

关键词： bionics biomimetics self-repair pneumatic structures Tensairity Aristolochia

Transcriptional Activation and Production of Tryptophan-derived Secondary Metabolites in Arabidopsis Roots Contributes to the Defense against the Fungal Vascular Pathogen Verticillium Iongisporum

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2012年第6期5卷 1389-1402页

作者： Tim Iven Stefanie Konig Seema Singh Susanna A. Braus-Stromeyer Matthias Bischoff Lutz F. Tietze Gerhard H. Braus Volker Lipka Ivo Feussner Wolfgang Droge-Laser Julius-Maximilians-Universitat Wurzburg Julius-von-Sachs-lnstitut Pharmazeutische Biologie Julius-von-Sachs-Platz 2 97082 Wurzburg Germany Georg-August-Universitat Albrecht-von-Haller-lnstitut fur Pflanzenwissenschaften Biochemie der Pflanze Justus-von-Liebig-Weg 11 37077 GottingenGermany Georg-August-Universitat Institut for Mikrobiologie und Genetik Molekulare Mikrobiologie und Genetik Grisebachstr. 8 37077 Gottingen Germany Georg-August-Universitat Institut for Organische und Biomolekulare Chemie AG Tietze Tammannstrasse 2 37077 Gottingen Germany Georg-August-Universitat Schwann-Schleiden-Forschungszentrum for Molekulare Zellbiologie Abteilung Zellbiologie der Pflanze Julia-Lermontowa-Weg3 37077 Gottingen Germany

The soil-borne fungal pathogen Verticillium Iongisporum causes vascular disease on Brassicaceae host plants such as oilseed rape. The fungus colonizes the root xylem and moves upwards to the foliage where disease symptoms become visible. Using Arabidopsis as a model for early gene induction, we performed root transcriptome analyses in re- sponse to hyphal growth immediately after spore germination and during penetration of the root cortex, respectively. Infected roots showed a rapid reprogramming of gene expression such as activation of transcription factors, stress-, and defense-related genes. Here, we focused on the highly coordinated gene induction resulting in the production of tryp- tophan-derived secondary metabolites. Previous studies in leaves showed that enzymes encoded by CYP81F2 and PEN2 （PENETRATION2） execute the formation of antifungal indole glucosinolate （IGS） metabolites. In Verticillium-infected roots, we found transcriptional activation of CYP81F2 and the PEN2 homolog PEL 1 （PEN2-LIKE1）, but no increase in antifungal IGS breakdown products. In contrast, indole-3-carboxylic acid （13CA） and the phytoalexin camalexin accumulated in infected roots but only camalexin inhibited Verticillium growth in vitro. Whereas genetic disruption of the individual metabolic pathways leading to either camalexin or CYP81F2-dependent IGS metabolites did not alter Verticillium-induced disease symptoms, a cyp79b2 cyp79b3 mutant impaired in both branches resulted in significantly enhanced susceptibility. Hence, our data provide an insight into root-specific early defenses and suggest tryptophan-derived metabolites as active anti- fungal compounds against a vascular pathogen.

关键词： Verticillium Iongisporum Arabidopsis root transcriptome camalexin indole glucosinolates.