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d(E/')TECTION de LA MUTATION dU G(E/')NE hMLh1 dANS LE CANCER COLO-RECTAL PAR(E/')LECTROPhOR(E/')SE CAPILAI-LRE ASSOCI(E/')E (A/') LA FLUORESCENCE LASER-INdUITE

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Journal of shanghai Second Medical University(Foreign Language Edition) 2006年第1期18卷 20-25页

作者：冯波郑民华陆爱国李健文江唯娜王明亮马君俊董峰石先哲许国旺 Service de chirurgiel'h(o|^)pital Ruijin l'université médicale numéro 2 de ShanghaiInstitut Chirurgical Digestif de ShanghaiShanghai 200025Chine institut physico-chimique de daliandalian 116011 Chine

Objectif L＇objectif de ce travail est d＇etablir une méthode d＇analyse du polymorphisme de la conformation monochaine （ PCMC ）, capable de détecter la mutation ponctuelle du géne hMLh1 dans le cancer colique en utilisant l＇ l ctrophor se capillaire associée a it la fluorescence laser-induite （FLI-EC）. Méthodes Les exons 12 du gdne hMLh1 du sang périphérique de 42 patients de cancer colo-rectal sporadique et 20 sujets sains témoins sont amplifies par la PCR. Le PCMC des produits du PCR est analysé par FLI-EC. Les échantillons anormaux ont été confirmés par séquen age EC. Les effets de la concentration du milieu （ LPA ） , de la temperature de séparationéet du voltage de séparation sur le comportement de EC ont été aussi etudiés. Résultats L＇analyse de PCMC par la methode FLI-EC a retrouvé une mutation hétérozygote chez 4 des 42 patients. La mutation ponctuelle de Tl l51A a été objectivée par la séquen age EC de ces échantillons, aucune mutation n＇a été retrouvée chez les 20 sujets sains témoins. La séparation des pics d＇AdN monochainaux a été facilitée par une légdre augmentation de la concentration de LPA （4%-6%）, une légdre baisse de la temperature de séparation （20℃） et une légére élévation du voltage de séparation （9kV）. Conclusion Une concentration adequate de LPA, une temperature de séparation appropriée et un voltage de séparation bien approprié améliorent considérablement l＇efficacité du FLI-EC. L＇application de cette méthode pour détecter la mutation ponctuelle du géne hMLh1 est d＇une rapiditY, d＇une efficacité, et d＇uneéreproductivit consid rables. Cette méthode de réalisation facile reste trés prometteur pour le dépistage rapide et massive des mutations génétiques.

关键词： électrophorèse électrique cancer colo-rectal hMLh1 polymorphisme de la conformation monochaine( PCMC) mutation génétique

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Tunable construction of transition metal-coordinated helicene cages

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Chinese Chemical Letters 2021年第12期32卷 3988-3992页

作者： Yongle ding Chengshuo Shen Fuwei Gan Jinghao Wang Guoli Zhang Lingling Li Mouhai Shu Bangshang Zhu Jeanne Crassous huibin Qiu School of Chemistry and Chemical Engineering Frontiers Science Center for Transformative MoleculesState Key Laboratory of Metal Matrix CompositesShanghai Jiao Tong UniversityShanghai 200240China Instrumental Analysis Center Shanghai Jiao Tong UniversityShanghai 200240China Univ Rennes Institut des Sciences Chimiques de RennesUMR CNRS 6226Campus de BeaulieuRennes 35042France

We report a facile and tailored method to prepare globally twisted chiral molecular cages through tunable coordination of bis-bipyridine-terminated helicene ligands to a series of transition metals including Fe(Ⅱ), Co(Ⅱ), Ni(Ⅱ) and Zn(Ⅱ). This system shows an efficient remote transfer of stereogenecity from the helicene core to the bipyridine-metal coordination sites and subsequently the entire cages. While the Fe(Ⅱ), Co(Ⅱ) and Ni(Ⅱ)-derived M_(2)L_(3)(M for metal and L for ligand) cages exhibit quasi-reversible redox features, the Zn(Ⅱ) analogues reveal prominent yellow circularly polarized luminescence. Interestingly,with the addition of Na_(2)SO_(4), the Zn_(2)L_(3)cages reassemble into sextuple-stranded Zn_(6)L_(6)(SO_(4))_(4)cages in which three Zn_(2)L_(2) units are bound together by four sulfates and further coalesced by offset inter-ligandπ-π interactions.

关键词： helicene Coordination cage Chiral Global helicity Circularly polarized luminescence

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Pyrene Excimer-based Bis-triazolyl Pyranoglycoligands as Specific Mercury Sensors

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Chinese Journal of Chemistry 2012年第12期30卷 2874-2878页

作者： he Xiaopeng Xie Juan Chen Guorong Chen Kaixian Key Laboratory for Advanced Materials & institute of Fine Chemicals East China University of Science and Technology Shanghai 200237 China PPSM Institut d'Alembert ENS de Cachan CNRS UMR 8531 61 Avenue du pt Wilson F-94235 Cachan France State Key Laboratory of drug Research Shanghai Institute ofMateria Medica Chinese Academy of Sciences Shanghai 201203 China

New C3,4-disubstituted bis-triazolyl glycoligands that feature a glucosyl or galactosyl scaffold incorporating two pyrenyl groups were synthesized via the Cu^1-catalyzed azide-alkyne 1,3-dipolar cycloaddition reaction （Cue-AAC）. These compounds exert a major emission band corresponding to that of pyrene excimer and respond specifically to mercury with a markedly quenched fluorescence. The epimeric nature of the pyranoglycosyl scaffold is determined influential toward the selectivity of the sensors.

关键词： glycoligand click chemistry sugar mercury chemosensor

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Terahertz Radiation from a Longitudinal Electric Field Biased Femtosecond Filament in Air

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Chinese Physics Letters 2020年第6期37卷 44-47页

作者： Yi Lin Shaojie Liu Aurélien houard AndréMysyrowicz Vladimir T.Tikhonchuk shanghai Key Lab of Modern Optical System University of Shanghai for Science and TechnologyShanghai 200093China Laboratoire d’Optique Appliquée ENSTA ParisEcole PolytechniqueCNRSInstitut Polytechnique de Paris828 Boulevard des Maréchaux91762 Palaiseau cedexFrance Centre Lasers Intenses et Applications University of Bordeaux-CNRS-CEA351 Cours de la Liberation33405 Talence cedexFrance ELI-Beamlines Institute of PhysicsCzech Academy of Sciences25241 Dolni BrezanyCzech Republic

The terahertz(Thz)temporal waveform and spectrum from a longitudinal electrically biased femtosecond filament is studied *** initial direction of the electron motion inside the unbiased filament plasma is deduced from the transformation of the Thz temporal waveform with applied fields of opposite ***,a spectrum shift to lower frequency of the Thz spectrum is observed in the presence of a biased *** agrees well with theoretical predictions.

关键词： field. spectrum opposite

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Far Infrared Resonance Transition Study of the Chain-End S=1/2 Modes in an S=1 Antiferromagnetic Chain

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Chinese Physics Letters 1995年第5期12卷 313-316页

作者： LU Wei ShEN Xuechu LIU Pulin M.von Ortenberg J.Tuchendler J.P.Renard ZhENG Fen National Laboratory for Infrared Physics Academia SinicaShanghai Institute of Technical PhysicsAcademia SinicaShanghai 200083 Fudan-T.d.Lee Physics Laboratory Department of PhysicsFudan UniversityShanghai 200433 humboldt University of Berlin Invalidenstraβe 1100-1040 BerlinF.R.Germany Lahoratoire de dispositiofs Infrarouge et Micro-Ondes Universite Paris VIF-75252 ParisFrance institut d’Electronique Fondamentale Universite Paris-SudF-91405 OrsayFrance department of Biology Shanghai Normal UniversityShanghai 200234

The unpaired spin S=1/2 states which occur at the ends of an S=1 heisenberg-like antiferromagnetic chain,have been observed recently in low-temperature electron spin-resonance measurements of Ni(C_(2)h_(8)N_(2))_(2)NO_(2)ClO_(4) containing selected *** present here a further study on this topic by far infrared transmission spectra under the magnetic *** splitting of S=1/2 modes was clearly observed and explained by the interaction of S=1/2 modes at two nearby chains,which can be separated by vacancy-like defect.

关键词： S=1/2 field. Transition

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Metamorphosis of Magnetospirillum magneticum AMB-1 cells

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Chinese Journal of Oceanology and Limnology 2010年第2期28卷 304-309页

作者：张风丽张葵赵三军肖天 Michel deNIS 吴龙飞 Laboratoire de Chimie Bactérienne UPR9043 Institut de Biologie Structurale et Microbiologie Laboratory of Marine Biotechnology School of Life Sciences & Biotechnology Key Laboratory of Microbial Metabolism Ministry of Education Shanghai Jiao Tong University Laboratoire de Microscopies et d’Etude de Nanostructures (LMEN) Université de Reims Key Laboratory of Marine Ecology & Environmental Sciences Institute of Oceanology Chinese Academy of Sciences Laboratoire de Microbiologie Géochimie et Ecologie Marines CNRS UMR6117 Centre d'Océanologie de Marseille Université de la Méditerranée

Magnetospirillum magneticum strain AMB-1 belongs to the family of magnetotactic bacteria. It possesses a magnetosome chain aligning, with the assistance of cytoskeleton filaments MamK, along the long axis of the spiral cells. Most fresh M. magneticum AMB-1 cells exhibit spiral morphology. In addition, other cell shapes such as curved and spherical were also observed in this organism. Interestingly, the spherical cell shape increased steadily with prolonged incubation time. As the actin-like cytoskeleton protein MreB is involved in maintenance of cell shapes in rod-shaped bacteria such as Escherichia coli and Bacillus subtilis, the correlation between MreB protein levels and cell shape was investigated in this study. Immunoblotting analysis showed that the quantity of MreB decreased when the cell shape changed along with incubation time. As an internal control, the quantity of MamA was not obviously changed under the same conditions. Cell shape directs cell-wall synthesis during growth and division. MreB is required for maintaining the cell shape. Thus, MreB might play an essential role in maintaining the spiral shape of M. magneticum AMB-1 cells.

关键词： Magnetotactic bacteria Magnetospirillum magneticum AMB-1 MreB

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distinct pathogenic mechanisms of various RARS1 mutations in Pelizaeus-Merzbacher-like disease

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Science China(Life Sciences) 2021年第10期64卷 1645-1660页

作者： Guang Li Gilbert Eriani En-duo Wang Xiao-Long Zhou State Key Laboratory of Molecular Biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of Sciences/University of Chinese Academy of SciencesShanghai 200031China Architecture et Réactivitéde l’ARN UPR9002 CNRSInstitut de Biologie Moléculaire et CellulaireUniversitéde Strasbourg67084 StrasbourgFrance School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China

Mutations of the genes encoding aminoacyl-tRNA synthetases are highly associated with various central nervous system *** mutations,including c.5A>G,p.d2G;c.1367C>T,p.S456L;c.1535G>A,p.R512Q and c.1846_1847del,p.Y616Lfs*6 of RARS1 gene,which encodes two forms of human cytoplasmic arginyl-tRNA synthetase(hArgRS),are linked to Pelizaeus-Merzbacher-like disease(PMLd)with unclear *** these mutations,c.5A>G is the most extensively reported mutation,leading to a p.d2G mutation in the N-terminal extension of the long-form ***,we showed the detrimental effects of R512Q substitution andΔC mutations on the structure and function of hArgRS,while the most frequent mutation c.5A>G,p.d2G acted in a different manner without impairing hArgRS *** nucleotide substitution c.5A>G reduced translation of hArgRS mRNA,and an upstream open reading frame contributed to the suppressed translation of the downstream main *** together,our results elucidated distinct pathogenic mechanisms of various RARS1 mutations in PMLd.

关键词： aminoacyl-tRNA synthetase(aaRS) central nervous system(CNS) protein biosynthesis translation initiation tRNA

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EFFECTS OF hUMAN BLOOd CONSTITUENTS ON FORMATION OF dNA-CARCINOGEN AddUCTS AFdG REVEALEd BY 32p-POSTLABELLING

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Chinese Science Bulletin 1990年第21期35卷 1827-1829页

作者：沈建华 M.WEGENKE T.WOLFF shanghai institute of Entomology Academia Siniea Shanghai 200025 PRC institut für Toxikologie Gesellschaft für Strahlen-und Umweltforschung D-8042 Neuherberg München FRG

The formation of dNA-adducts, i.e. the covalent modification of dNA molecules with carcinogens, is the initial event in carcinogenesis. In the organisms, most of the genotoxic chemicals must be first converted into el... 详细信息

关键词： dNA-adducts 32p-postlabelling 2-aminofluorene(2-AF) human blood.

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Seasonal metal fluxes derived by the interaction of surface water and groundwater in an aquaculture estuary

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海洋学报:英文版 2023年第8期42卷 113-124页

作者： Xiaoxiong Wang Jordi Garcia-Orellana Xiaogang Chen Jianan Liu Fenfen Zhang Jianguo Qu Zhuoyi Zhu Jinzhou du State Key Laboratory of Estuarine and Coastal Research East China Normal UniversityShanghai 200062China ICTA-UAB Institut de Ciència i Tecnologia AmbientalsUniversitat Autònoma de BarcelonaBarcelona E-08193Spain departament de Física Universitat Autònoma de BarcelonaBarcelona E-08193Spain Key Laboratory of Coastal Environment and Resources of Zhejiang Province School of EngineeringWestlake UniversityHangzhou 310024China School of Oceanography Shanghai Jiao Tong UniversityShanghai 200030China

Submarine groundwater discharge(SGd)plays a major role as a conveyor of metals to coastal ***,the seasonal change of metal fluxes derived through SGd is ***,we evaluated the behaviours and fluxes of trace metals(Mn,Fe,Ba,Pb,U,Cr,Zn,Cu)in an estuary under different seasonal *** behaviours of trace metals revealed that SGd was the source of Mn(3.51 mmol/(m^(2)·d)),Fe(0.174 mmol/(m^(2)·d))and Ba(0.024 mmol/(m^(2)·d)),but the Cu sink(−0.55μmol/(m^(2)·d))and other metals exhibited a seasonal source‒sink *** seasonal variation of dissolved organic matter and the fresh groundwater proportion in subterranean estuaries may have an important effect on metals fluxes especially for the Fe,Mn and *** result shows that the single seasonal metal fluxes estimation applied to the annual scale will cause a large deviation,up to 3.6 times for Fe,5.5 times for Mn,and 15 times for ***,the influence of seasonal fluctuations on SGdderived metal fluxes cannot be ignored,and our findings will be important for comprehending the metal budget and cycle in nearshore environment.

关键词： subterranean estuary seawater intrusion heavy metals seasonal changes coastal management

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Genetic Analysis of ele Mutants and Comparative Mapping of ele1 Locus in the Control of Organ Internal Asymmetry in Garden Pea

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Journal of Integrative Plant Biology 2010年第6期52卷 528-535页

作者： Xin Li Li-Li Zhuang Mike Ambrose M. Phil. Catherine Rameau Xiao-he hu Jun Yana da Luo School of Life Science and Biotechnology Shanghai JiaoTong University Shanghai 200030 China department of Crop Genetics John Innes Centre Colney Norwich NR4 7UH United Kingdom Station de Genetique et d'Amelioration des Plantes Institut J.P. Bourgin UR 254 INRA Versailles France National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biologica Sciences the Chinese Academy of Sciences Shanghai 200032 China

Previous study has shown that during zygomorphic development in garden pea （Pisum sativum L.）, the organ internal （IN） asymmetry of lateral and ventral petals was regulated by a genetic locus, SYMMETRIC PETAL 1 （SYP1）, while the dorsoventral （dV） asymmetry was determined by two CYC-like TCP genes or the PsCYC genes, KEELEd WINGS （K） and LOBEd STANdARd 1 （LST1）. In this study, two novel loci, ELEPhANT EAR-LIKE LEAF 1 （ELE1） and ELE2 were characterized. These mutants exhibit a similar defect of IN asymmetry as syp1 in lateral and ventral petals, but also display pleiotropic effects of enlarged organ size. Genetic analysis showed that ELE1 and ELE2 were involved in same genetic pathway and the enlarged size of petals but not compound leaves in e/e2 was suppressed by introducing k and Ist1, indicating that the enlargement of dorsal petal in e/e2 requires the activities of K and LST1. An experimental framework of comparative genomic mapping approach was set up to map and clone LjELE1 locus in Lotus japonicus. Cloning the ELE1 gene will shed light on the underlying molecular mechanism during zygomorphic development and further provide the molecular basis for genetic improvement on legume crops.

关键词： GENETIC research PEAS GENETICS FOLIAR diagnosis LOCUS (Genetics) COMPARATIVE genomic hybridization LEGUMES

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