检索结果-南通市图书馆

American Journal of Plant Sciences 2018年第10期9卷 2053-2062页

作者： Elisabeth Zongo Bouma James Néya Valentin Stanislas Edgar Traoré Essowé Palanga Jean Zabré Nicolas Barro Oumar Traoré Laboratoire de Virologie et de biotechnologie Végétale (LVBV)/institut de l’Environnement et de Recherches Agricoles (INERA) & Laboratoire Mixte Internationale (LMI) Patho-Bios/INERA Ouagadougou Burkina Faso Université Ouaga I Professeur Joseph Ki-Zerbo Ouagadougou Burkina Faso Laboratoire National de Biosécurité Ouagadougou Burkina Faso

Bambara groundnut [Vigna subterranean (L.) Verdc] is a food legume with high potential for food security in Sub-Saharan Africa. However, in addition to being a neglected crop, its production is limited by several constraints among which viral diseases are most cited. In order to contribute to the improvement of Bambara groundnut in Burkina Faso, local accessions of the crop were screened for resistance to Cowpea mottle virus (CPMoV), one of the most damaging viruses in grain legumes. Seven local accessions (C1 to C7) from two agro-ecological zones were evaluated by mechanical inoculation in field conditions in 2016 and 2017. The infected plants exhibited various symptoms of chlorosis, leaf deformation, growth retardation and plant stunting. CPMoV caused a significant reduction in the number of flowers and pods. As a result, grain yield was reduced by 49.5% to 83.9% depending on the accessions. The impact of the virus in yield loss was lowest in accessions C6 and C7 which indicated their possible used in the management of Cowpea mottle virus disease in bambara groundnut.

关键词： Bambara Groundnut Cowpea mottle virus Impact Yield Loss

Molecular Characterization of a Large Diversity of Bambara Groundnut Potyviruses (Vigna subterranea(L.) Verdc.) in Burkina Faso

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advances in Bioscience and Biotechnology 2019年第9期10卷 270-281页

作者： Aboubié Elisabeth Zongo Bouma James Néya Essowé Palanga Drissa Sérémé Moustapha Koala Régis Dimitri Sokpé Longué Jean Zabré Zakaria Bouda Nicolas Barro Oumar Traoré Laboratoire de Virologie et de biotechnologie Végétale (LVBV)/institut de l’Environnement et de Recherches Agricoles (INERA) & Laboratoire Mixte Internationale (LMI) Patho-Bios/INERA Ouagadougou Burkina Faso Université Ouaga I Professeur Joseph Ki-Zerbo Ouagadougou Burkina Faso Laboratoire National de Biosécurité Ouagadougou Burkina Faso

Potyviruses are major constraints to grain legume production by causing significant yield losses. Potyviruses infecting Bambara groundnut (Vigna subterranea) were investigated in Burkina Faso. Leaf samples collected from three agroclimatic zones were subjected to RT-PCR and sequence analyses. Of a total of 135 samples, 36 (26.67%) were detected positive in RT-PCR tests using potyvirus universal primers. Analysis of full coat protein (cp) sequences from 24 isolates revealed the occurrence of three groups of Bambara groundnut-infecting potyviruses. Virus isolates in group 1 shared 94.5% - 100% nucleotide (nt) identity with CABMV whereas those in group 2 and group 3 were distantly related Bean common necrosis virus (BCMNV) and Passiflora virus Ugandan which were their respective closest potyviruses. Group 2 shared 77.1% nt and 78.8% - 79.9% aa identity with BCMNV and group 3 shared 77.3% - 78.3% nt and 80.7% - 81.5% aa identity with Passiflora virus Ugandan. All three groups were confirmed by phylogenetic analyses. Taking into account potyvirus demarcation criteria, group 1 isolates belonged to CABMV species. Group 2 and group 3 were assigned to a potentially new potyviruses species and designated Bambara groundnut potyvirus 1 (BGPV1) and Bambara groundnut potyvirus 2 (BGPV2).

关键词： Bambara Groundnut Potyvirus Characterization Coat Protein Sequence Diversity

Metagenomic analysis reveals wide distribution of phototrophic bacteria in hydrothermal vents on the ultraslow-spreading Southwest Indian Ridge

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Marine Life Science & Technology 2022年第2期4卷 255-267页

作者： Hong Chen deng Hui Li Ai Jun Jiang Xue Gong Li Shi Jun Wu Jian Wei Chen Meng Jie Qu Xiao Qing Qi Jie Dai Rui Zhao Wei-Jia Zhang Shan Shan Liu Long-Fei Wu Laboratory of deep Sea Microbial Cell Biology Institute of Deep Sea Science and EngineeringChinese Academy of SciencesSanya572000China University of Chinese Academy of Sciences Beijing100864China France-China Joint Laboratory for Evolution and development of Magnetotactic Multicellular Organisms(LIA-MagMC) MarseilleFrance/SanyaChina institution of deep-Sea Life Sciences IDSSE-BGIIDSTI-CAS/Hainan Deep-Sea Technology LaboratorySanya/ShenzhenChina BGI-Qingdao BGI-ShenzhenQingdao266555China Zhejiang University Hangzhou310027China BGI-Shenzhen Shenzhen518083China Qingdao-Europe Advanced institute for Life Sciences BGI-ShenzhenQingdao266555China Aix Marseille University Centre national de la recherche scientifiqueLaboratoire de Chimie BactérienneInstitut de Microbiologie de la MéditerranéeL’Institut MicrobiologieBioénergies et Biotechnologie13402MarseilleFrance

deep-sea hydrothermal vents are known as chemosynthetic ***,high temperature vents emit light that hypothetically can drive photosynthesis in this *** studies have sporadically reported the occurrence of phototrophic populations such as cyanobacteria in hydrothermal *** determine how geographically and taxonomically widespread phototrophs are in deep-sea hydrothermal vents,we collected samples from three niches in a hydrothermal vent on the Southwest Indian Ridge and carried out an integrated metagenomic *** determined the typical community structures of microorganisms found in active venting fields and identified populations of known potential chlorophototrophs and *** chlorophyll biosynthetic pathways were identified in all *** contrast,proteorhodopsins were only found in active beehive smoker *** groups possessing potential phototrophy dependent on semiconductors present in hydrothermal vents were also found in these *** systematic comparative metagenomic study reveals the widespread distribution of phototrophic bacteria in hydrothermal vent *** results support the hypothesis that the ocean is a seed bank of diverse *** vent light may provide energy and confer a competitive advantage on phototrophs to proliferate in hydrothermal vent ecosystems.

关键词： Chlorophyll Rhodopsin Phototrophic bacteria Hydrothermal vent Southwest Indian Ridge

Roots and rhizomes of wild Asparagus:Nutritional composition,bioactivity and nanoencapsulation of the most potent extract

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food Bioscience 2022年第1期45卷 349-360页

作者： Khaoula Adouni Ana Júlio Celestino Santos-Buelga Ana M.Gonzalez-Paramas Patrícia Filipe Patricia Rijo Sofia A.Costa Lima Salette Reis Angela Fernandes Isabel C.F.R.Ferreira Virginia Fernandez-Ruiz Patricia Morales Guido Flamini Lotfi Achour Pedro Fonte Laboratoire de Recherche“Bioressources:Biologie Integrative&Valorisation” Institut Superieur de Biotechnologie de MonastirAvenue Tahar HaddedBP 74Universite de Monastir5000MonastirTunisia CBIOS-Research Center for Biosciences&Health Technologies Universidade Lusofona de Humanidades e TecnologiasCampo Grande 3761749-024LisbonPortugal department of Biomedical Sciences University of Alcal´aCtra.Madrid-Barcelona Km.33.60028871Alcala de HenaresMadridSpain Grupo de Investigacion en Polifenoles(GIP-USAL) Facultad de FarmaciaUniversidad de SalamancaCampus Miguel de UnamunoE-37007SalamancaSpain Research institute for Medicines(iMed.ULisboa) Faculty of PharmacyUniversidade de LisboaAv.Professor Gama Pinto1649-003LisboaPortugal LAQV REQUIMTEDepartment of Chemical Sciences–Applied Chemistry LabFaculty of Pharmacy10 University of PortoRua Jorge de Viterbo Ferreira 2284050-313PortoPortugal Centro de Investigacao de Montanha(CIMO) Instituto Politecnico de BragancaCampus de Santa Apolonia5300-253BragancaPortugal department of Nutrition and Food Science Faculty of PharmacyComplutense University of Madrid(UCM)Plaza Ramon y Cajal s/n28040MadridSpain Dipartimento di Farmacia University of Pisavia Bonanno 656126PisaItaly Centro Interdipartimentale di Ricerca“Nutraceutica e Alimentazione per la Salute”University of Pisa Via del Borghetto 8056124PisaItaly Center for Marine Sciences(CCMar) University of AlgarveGambelas Campus8005-139FaroPortugal department of Chemistry and Pharmacy Faculty of Sciences and TechnologyUniversity of AlgarveGambelas Campus8005-139FaroPortugal iBB-institute for Bioengineering and Biosciences Department of BioengineeringInstituto Superior TecnicoUniversidade de Lisboa1049-001LisboaPortugal Associate Laboratory i4HB—institute for Health and Bioeconomy at instituto superior Tecnico Universidade de LisboaAv.Rovisco Pais1049-001LisboaPortugal

The nutritional composition and bioactive properties of roots and rhizomes of Asparagus stipularis were *** activity of extracts obtained by infusion was evaluated using free radicals scavenging and reducing power *** liver primary cell was used to check the hepatotoxicity of *** revealed that Asparagus samples are likely a source of nutrients,such as dietary fibre and essential fatty ***-DAD-ESI/MS characterization of infusions allowed the identification and quantitation of 7 phenolic compounds,all hydroxycinnamoyl derivatives,with caffeic acid as the most *** infusion contained the highest amounts of these *** also exhibited the highest antioxidant activity in all assays,with EC_(50) values of 0.44±0.01,0.98±0.03 and 0.64±0.01 mg/mL for DPPH,ABTS and FRAP assays,respectively,with no toxicity towards PLP2 primary cell cultures(GI_(50)>400μg/mL).PLGA nanoparticles loaded with root extract were prepared using solvent-evaporation double emulsion *** size was about 260 nm and a polydispersity index around 0.1,with a zeta potential of about-36 mV,as well as a good encapsulation efficiency of approximately 83%.Their morphology was analysed by SEM and spherical polymeric nanoparticles with a smooth surface were *** and DSC were also performed,which allowed corroborating the efficacy of the encapsulation and to confirm the production of a stable and robust system to load Asparagus *** developed nanoparticles are expected to be used as delivery systems for bioactive compounds of *** and they could be used as an innovative dietary supplement.

关键词： Asparagus stipularis Nutritional composition Antioxidant activity Nanoparticles PLGA

Microbiological Quality of Fresh and Grilled Mutton Sold in Ouagadougou, Burkina Faso

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food and Nutrition Sciences 2022年第12期13卷 986-1000页

作者： Zoénabo Douamba Abel Tankoano Donatien Kaboré Diarra Compaore-Sereme Mahamoudou Ouédraogo Pingdwindé Marie Judith Samadoulougou-Kafando Adama Paré Mamadou Hama Dicko Hagrétou Sawadogo/Lingani institut de Recherche en Sciences Appliquées et Technologies (IRSAT)/Centre National de la Recherche Scientifique et Technologique (CNRST) Ouagadougou Burkina Faso institut de Recherche en Sciences Appliquées et Technologies (IRSAT)/Centre National de la Recherche Scientifique et Technologique (CNRST) Bobo-Dioulasso Burkina Faso Laboratoire de Biochimie Biotechnologie Technologie Alimentaire et Nutrition (LABIOTAN) UFR/SVT Université Joseph KI-ZERBO Ouagadougou Burkina Faso

In Ouagadougou, grilled meats in the form of pieces and brochettes are very popular and well-known to consumers. The aim of this study was to assess the microbial quality of mutton meat sold in Ouagadougou, Burkina Faso. A total of 120 samples were collected from 20 meat grillers 60 samples of fresh meat and 60 samples of grilled meat. The sampling was done between the month of August 2018 and the month of February 2019. The samples were analyzed according to standard methods. The test performed were counts of Aerobic Mesophilic Bacteria (AMB), yeasts and molds, enterobacteria, Campylobacter spp, Staphylococcus aureus, Bacillus cereus, Brucella spp, Pseudomonas aeruginosa, the search for salmonella, and the detection of antibiotics residues. Results showed a high count of AMB (8.77 and 6.78 log UFC/g);enterobacteria (6.58 and 3.05 log UFC/g), Staphylococcus aureus (6.45 and 4.35 log UFC/g), Bacillus cereus (6.98 and 4.52 log UFC/g), Campylobacter (6.03 and 3.86 log UFC/g), yeasts and molds (4.80 and 3.26 log UFC/g) and Pseudomonas aeruginosa (0.45 and 0.15 log UFC/g), respectively in fresh meat and grilled meat. Presumptive Salmonella was found in 95% of fresh meat samples and in 75% of grilled meat samples. In the tested samples, no Brucella spp were detected. However, residues of antibiotics were found in 5% of fresh meat samples and 5% of grilled meat samples. Means of moisture and pH were respectively 74.91% and 6.05% for fresh meat and 53.21% and 6.06% for grilled meat. The average microbial counts recorded in fresh and grilled meat are significantly high and indicate poor hygiene in the raw material and ready-to-eat meat. Good practices of hygiene and processing guides should be developed for the meat grilling value chain actors to reduce contamination risks.

关键词： Meat Mutton Grilling Microbial Quality

Variability in patterns of macro-epiphytic leaf community of Posidonia oceanica in the Islands of Kuriate:Western coast of Tunisia

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Coastal Life Medicine 2016年第3期4卷 211-216页

作者： Ben Brahim Mounir Abdennahder Moufida Feki-Sahnoun Wafa Mahfoudi Mabrouka Hamza Asma Faculty of Sciences of sfax Road of Soukra km 4BP 802-3038 SfaxTunisia National institut of Sciences and Technology of the Sea Center of SfaxBP 1035-CP 3018 SfaxTunisia

Objective:To test the response of the epiphyte community structure and biomass of the Posidonia oceanica(***)leaves to natural ***:Sampling of *** was carried in winter and summer on three sites in Kuriate Islands(western coast of Tunisia)subject to different environments *** of *** were preserved in seawater-formalin(5%)solution for macro-epiphytes species identification in the *** samples were examined for leaf surface per shoot and the coverage(expressed as a percentage of leaf surface)of each morphological group,then carefully scraped with a razor *** and scraped leaves were oven-dried at 60℃for 48 *** was expressed as g dry weight/***:The biomass and the percentage cover of macro-epiphytic leaves showed seasonal *** highest values of epiphytic leaves were detected in summer whereas the lowest values were registered during *** showed that Kuriate Islands functioned as a single ecosystem in terms assemblage of macro-epiphytic leaves since no significant variation was detected for biomass and percentage cover at the scale *** study showed that natural disturbance had no effect on the assemblage distribution and the biomass of macro-epiphyte on the leaves of *** between the scales of site,whereas variability at the smallest scale was *** showed that exposure to wind and current had no effect on the biomass of macro-epiphytes ***:Biomass and assemblages of macro-epiphytic leaves of *** were high in summer and homogenous between all sites *** disturbances such as exposure to wind have no effect on the distribution and the biomass of epiphytes on the shallow meadow.

关键词： Macro-epiphyte Assemblage Variability Posidonia oceanica Tunisia

Synthesis of New 2-Phenylamino-4H-chromene-3-carbonitrile derivatives and Their Effects on Tumor Cell Lines and against Protein Kinases

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Organic Chemistry 2020年第2期10卷 88-103页

作者： Ali Bouattour Mehdi Fakhfakh Souhir Abid Ludovic Paquin Rémy Le Guével Thierry Charlier Sandrine Ruchaud Stéphane Bach Jean-Pierre Bazureau Houcine Ammar Laboratoire de Chimie Appliquée: Hétérocycles Corps Gras et Polymères Faculté des Sciences de Sfax Université de Sfax Sfax Tunisie Département de Chimie Collège des Sciences et des Arts Université de Jouf Al Qurayyat KSA institut des Sciences Chimiques de Rennes (ISCR) UMR CNRS 6226 Université de Rennes 1 Campus de Beaulieu Rennes France S2 Wave Platform ScanMAT UMS 2001CNRS Université de Rennes 1 Campus de Beaulieu Rennes France Université de Rennes 1 ImPACcell Platform Campus Villejean Rennes France Sorbonne Universités UPMC Université Paris 06 CNRS USR3151 Protein Phosphorylation and Human Disease Unit KISSf Platform Station Biologique Place Georges Teissier Roscoff France

The synthesis of 2-phenylimino-4H-chromene-3-carbonitriles 6(a-d) in good overall yields using an efficient and practical methodology in 3 steps has been implemented in this present work. The first step was a heterocyclization between 2-hydroxybenzaldehyde 1 and propanedinitrile 2 which produced 2-iminocoumarin 3 which was submitted to nitrogen/nitrogen displacement in the presence of aromatic primary amine 4. In the third step, reduction of 5 led to the desired 2-phenylimino-4H-chromene-3-carbonitriles 6. Compounds 5(a-d) and 6(a-d) were evaluated for their potential in vitro cytotoxicity against six selected tumor cell lines (Huh7-D12, Caco2, MDA-MB231, HCT 116, PC3 and NCI-H727) and tested for their protein kinase inhibition on eight selected protein kinases. Among them, compounds 5c and 6b exhibited inhibition on HsCK1e (5c: 44% and 6b: 42% at 1 μM) and 5c for cytotoxicity on PC3 cell lines (63% at 25 μM).

关键词： Iminocoumarin 2H-benzopyran 2-Imino-2H-benzopyran 4H-chromene 2-Amino-4H-chromene Nitrogen/Nitrogen Displacement Protein Kinase Inhibition Cytotoxicity

The Calcineurin B-Like Ca2＋ Sensors CBL1 and CBL9 Function in Pollen Germination and Pollen Tube Growth in Arabidopsis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2013年第4期6卷 1149-1162页

作者： Anette Maihs Leonie Steinhorst Jian-Pu Han Li-Ke Shen Yi Wang Jorg Kudla institut for Biologie und biotechnologie der Pflanzen Universitat MOnster Schlossplatz 4 48149 MOnster Germany State Key Laboratory of Plant Physiology and Biochemistry College of Biological Sciences National Plant Gene Research Center (Beijing) China Agricultural University Beijing 100193 China

Ca2＋ has been established as an important second messenger regulating pollen germination and tube growth. However, to date, only a few signaling components have been identified to decode and relay Ca2＋ signals in growing pollen tubes. Here, we report a function for the calcineurin B-like （CBL） Ca2＋ sensor proteins CBL1 and CBL9 from Arabidopsis in pollen germination and tube growth. Both proteins are expressed in mature pollen and pollen tubes and impair pollen tube growth and morphology if transiently overexpressed in tobacco pollen. The induction of these phenotypes requires efficient plasma membrane targeting of CBL1 and is independent of Ca2＋ binding to the fourth EF-hand of CBL1. Overexpression of CBL1 or its closest homolog CBL9 in Arabidopsis renders pollen germination and tube growth hypersensitive towards high external K＋ concentrations while disruption of CBL1 and CBL9 reduces pollen tube growth under low K~ conditions. Together, our data identify a crucial function for CBL1 and CBL9 in pollen germination and tube growth and suggest a model in which both proteins act at the plasma membrane through regulation of K＋ homeostasis.

关键词： calcium CBL pollen tube growth potassium

Volatile Compounds Analysis of Tunisian Propolis and Its Antifungal Activity

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Biosciences and Medicines 2018年第6期6卷 115-131页

作者： Ayari Jihene Ines Jabri Karoui Ayed Ameni Majdi Hammami Manef Abderrabba Laboratoire Matériaux Molécules et Applications Institut Préparatoire des Etudes Scientifiques et Techniques IPEST La Marsa Tunisia Laboratoire des Substances Bioactives Centre de Biotechnologie’a la Technopole de Borj-Cedria (CBBC) Hammam-Lif Tunisia

In this study, four samples of Tunisian propolis were analyzed for their volatile compounds. Static Headspace technique coupled with gas chromatography-mass spectrometry (GC/MS) was used for the volatile profile characterization. Statistical investigation of the samples was made applying hierarchical clustering method, K-Means cluster and principal component analysis. Results showed that 47, 36, 30 and 24 different compounds were identified, respectively, in the samples from Zouarine, Zelligua, Bizerte and Beni Khalled. Propolis volatiles were dominated by monoterpene hydrocarbons. α-pinene was the major compound representing 81.14%, 82.67% and 90.74%, respectively, of the total propolis volatiles collected from Zelligua, Beni Khalled and Zouarine and only 45.22% of the sample from Bizerte which had a very different composition. The in vitro antifungal activity of the volatiles from all samples against Candida albicans was also assayed and reported.

关键词： Propolis Headspace/GC-MS α-Pinene PCA Antifungal Activity

3-(Tetrazol-5-yl)-2-imino-coumarins derivatives: Synthesis, Characterization, and Evaluation on Tumor Cell Lines

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Organic Chemistry 2021年第1期11卷 24-34页

作者： Ali Bouattour Mehdi Fakhfakh Souhir Abid El-Gharbi Majdi Abid Ludovic Paquin Rémy Le Guevel Thierry Charlier Houcine Ammar Jean-Pierre Bazureau Laboratoire de Chimie Appliquée: Hétérocycles Corps Gras et Polymères Faculté des Sciences de Sfax Université de Sfax Sfax Tunisie Chemistry department College of Science and Arts Jouf University Al Qurayyat Al Jawf KSA institut des Sciences Chimiques de Rennes ISCR UMR CNRS 6226 Campus Beaulieu Université de Rennes 1 Bat. 10A Rennes Cedex France S2Wave Platform ScanMAT UMS 2001 CNRS Campus Beaulieu Université de Rennes 1 Bat. 10A Rennes Cedex France ImPACcell Platform SFR Biosit Bat. 8 Campus Villejean Rennes Cedex France

The first report of new 3-(tetrazol-5-yl)-2-iminocoumarin derivatives is described. The title compounds were prepared in two steps and were obtained in good yields (55-93%). They have been fully characterized by sup>1sup>H, sup>13sup>C NMR, FTIR, UV-Visible and HRMS. They were tested for their antiproliferative activities against six representative human tumor cell lines (Huh 7-D12, Caco2, MDA-MB231, HCT 116, PC3 and NCI-H727) and HaCat keratinocytes. Among them, compound 5e was active on HCT 116 (IC50 15 μM).

关键词： 2-Iminocoumarin Tetrazole Fluorescence Tumor Cell Lines HCT 116