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Deep learning methods for biotic and abiotic stresses detection and classification in fruits and vegetables:State of the art and perspectives

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Artificial Intelligence in Agriculture 2023年第3期9卷 46-60页

作者： Sèton Calmette Ariane Houetohossou Vinasetan Ratheil Houndji Castro Gbêmêmali Hounmenou Rachidatou Sikirou Romain Lucas Glele Kakaï Laboratoire de Biomathématiques et d'Estimations Forestières Faculty of Agronomic SciencesUniversity of Abomey-Calavi04 BP 1525CotonouBenin institut de Formation et de Recherche en Informatique University of Abomey-Calavi01 BP 526CotonouBenin Laboratoire de Défense des Cultures Centre de Recherches Agricoles d'AgonkanmeyInstitut National des Recherches Agricoles du Bénin(INRAB)01 BP 884CotonouBenin

Deep Learning(DL),a type of Machine Learning,has gained significant interest in many fields,including *** paper aims to shed light on deep learning techniques used in agriculture for abiotic and biotic stress detection in fruits and vegetables,their benefits,and the challenges faced by *** papers were collected from Web of Science,Scopus,Google Scholar,Springer,and Directory of Open Access Journals(DOAJ)using combinations of specific keywords such as:’Deep Learning’OR’Artificial Intelligence’in combination with fruit disease’,vegetable disease’,‘fruit stress',OR‘vegetable stress'following PRISMA *** the initial 818 papers identified using the keywords,132 were reviewed after excluding books,reviews,and the *** recovered scientific papers were from 2003 to 2022;93%addressed biotic stress on fruits and *** most common biotic stresses on species are fungal diseases(grey spots,brown spots,black spots,downy mildew,powdery mildew,and anthracnose).Few studies were interested in abiotic stresses(nutrient deficiency,water stress,light intensity,and heavy metal contamination).Deep Learning and Convolutional Neural Networks were the most used keywords,with GoogleNet(18.28%),ResNet50(16.67%),and VGG16(16.67%)as the most used ***-two percent of the data used to compile these models come from the fields,followed by data obtained *** problems due to unbalanced classes and the small size of some databases were also *** provided the research gaps and some perspectives from the reviewed *** research works are required for a deep understanding of the use of machine learning techniques in fruit and vegetable studies:collection of large datasets according to different scenarios on fruit and vegetable diseases,evaluation of the effect of climatic variability on the fruit and vegetable yield using AI methods and more abiotic stress studies.

关键词： Deep learning Prediction Fruits Vegetables Stress Agricultural yield

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Morphological Diversity of Corn’s (<i>Zea mays</i>L.) Local Cultivar and Improved Varieties in Central and North of Benin

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American Journal of Plant Sciences 2015年第18期6卷 2867-2877页

作者： Hafiz A. Salami Adolphe Adjanohoun Wilfrid Padonou Abdoul-Madjidou Yacoubou Djima Aly Chabi Yallou Haziz Sina Lamine Baba-Moussa Laboratoire de Biologie et de Typage Moléculaire en Microbiologie Département de Biochimie et de Biologie Cellulaire Faculté des Sciences et Techniques Université d’Abomey-Calavi Cotonou Bénin Centre de recherches agricoles Sud Institut National des Recherches Agricoles du Bénin (INRAB) Attogon Benin Programme Technologie Agricole et Alimentaire (PTAA) Institut National des Recherches Agricoles du Bénin (INRAB) Porto-Novo Benin Centre de recherches agricoles Nord Institut National des Recherches Agricoles du Bénin (INRAB) N’dali Benin

The better knowledge of a plant genetic biodiversity is based on a prior study of its agro-morphological characteristics. The objective of this study was to investigate the morphological diversity of maize accessions and its structure on the basis of 14 variables. For the experimentation, 43 and 98 maize accessions were planted in three replicates respectively in central and northern Benin following an incomplete randomized block. The mixed model analysis of two factors variance revealed a significant difference for all accessions considering each agro-morphological characteristic evaluated except the germination day of two areas seed accessions. The numerical classification grouped the accessions into four groups in each zone. The stepwise discriminant analysis showed that early characters related to plant height and ear insertion were the variables that discriminated accessions in both zones. Maturity and recovery ears, sensitivity to streak and the germination days are the variables that discriminate accessions of the two areas. These results provide a database for the creation of improved maize varieties that meet the needs of

关键词： Accessions Characterization Agro-Morphological Zea mays L. Benin

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Genetic Diversity of Maize Accessions (Zea mays L.) Cultivated from Benin Using Microsatellites Markers

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American Journal of Molecular Biology 2016年第1期6卷 12-24页

作者： Hafiz A. Salami Kamirou Chabi Sika Wilfrid Padonou Djima Aly Chabi Yallou Adolphe Adjanohoun Simeon Kotchoni Lamine Baba-Moussa Laboratoire de Biologie et de Typage Moléculaire en Microbiologie Département de Biochimie et de Biologie Cellulaire Faculté des Sciences et Techniques Université d’Abomey-Calavi Cotonou Bénin Programme de Technologie Agricole (PTAA) Institut National des Recherches Agricoles du Bénin (INRAB) Porto-Novo Bénin Centre de recherches agricoles Sud Institut National des Recherches Agricoles du Bénin (INRAB) Cotonou Bénin Centre de recherches agricoles Nord Institut National des Recherches Agricoles du Bénin (INRAB) Ina Bénin Departement of Biology Rutgers University Camden USA

Maize (Zea mays L.) is the major cereal cultivated in Benin and it is important to know its genetic diversity to improve the yield. The genetic markers of important traits are evaluated in order to improve the maize inbred lines. The aim of this study was to evaluate the genetic diversity of Benin’s maize accessions by SSR marker. Thus, one hundred eighty seven maize accessions from three areas (South, Center and North) were analyzed using three SSR markers. A total of 227 polymorphic bands were produced and showed high genetic diversity (Shannon index = 0.51). The polymorphic information content (PIC) values for the SSR loci ranged from 0.58 to 0.81, with an average of 0.71. Genetic distance-based UPGMA dendrogram showed a genetic differentiation between accessions and they were grouped into four clusters in each area. This work provides necessary information that can be used not only to improve the maize production and conservation but also to better manage genetic species resources in Benin.

关键词： SSR PCR Molecular Characterization LZea mays L. Benin

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Crystal structure and infrared spectrum of thallium holmium polyphosphate, TlHo(PO_3)_4

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Journal of Rare Earths 2008年第6期26卷 765-769页

作者： Karima Horchani-Naifer Jaouher Amami Mokhtar Férid Unité de recherches de Matériaux de Terres Rares Centre National de Recherches en Sciences des Matériaux

Crystals of thallium-holmium polyphosphate T1Ho（PO3）4 were grown by flux method technique and characterized by single crystal X-ray diffraction. Structure of T1Ho（PO3）4 was solved for the first time, and it crystallized in the monoclinic P21/n space group with the following unit-cell dimensions： a=1.02225（3） nm, b=0.88536（2） nm, c=1.09541（4） nm, β=105.888（1）°, V=0.95354（5） nm^3 and Z=4. The crystal structure was solved from 2174 independent reflections with final R1（F^2）=0.0442 and Rw（F^2）=0.0861 refined with 164 parameters. The atomic arrangement could be described as a long chain polyphosphate organization. Holmium atoms had eightfold coordination. The structure of T1Ho（PO3）4 consisted of HoO8 polyhedra sharing oxygen atoms with phosphoric group PO4. Infrared spectrum was investigated at room temperature in the frequencies range, 350--4000 cm^-1, showing some characteristic vibration bands of infinite chain structure of PO4 tetrahedra linked by bridging oxygen.

关键词： polyphosphate holmium crystal structure infrared spectroscopy rare earths

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Synthesis and Crystal Structure of a New Potassium Yttrium Diphosphate Dihydrate KYP_2O_7·2H_2O

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Chinese Journal of Structural Chemistry 2008年第10期27卷 1199-1204页

作者： Férid M Horchani-Naifer K Unité de recherches de Matériaux de Terres Rares Centre National de Recherches en Sciences des MatériauxB.P.95 Hammam-Lif 2050Tunisie

The dihydrated potassium yttrium diphosphate, KYP2O7·2H2O, has been synthesized for the first time. Single-crystal XRD study has allowed the determination of its structure and correct formula. It crystallizes in the monoclinic system, space group P21/c, with the cell parameters a = 7.7069（3）, b = 10.5801（4）, c = 10.0204（5）A,β = 93.24（3）A, V= 815.76（6）A^3, Z= 4, Mr = 337.98, F（000） = 656, Dc = 2.752 g/cm^3 and/t = 8.073 mm^-1. The single-crystal structure was solved from 1856 unique reflections with the final R = 0.0365 and wR = 0.0772, refined with 119 parameters. The atomic arrangement of KYP2O7·2H2O is built by the P2O7 groups, the YO7 and the KO10 polyhedrons which are connected by corner and edge-sharing to form a three-dimensional framework. Water molecules are directly bonded to the Y and K atoms, and they are located in channels running along the a direction.

关键词： crystal structure yttrium diphosphate infrared spectrum TGA-DTA analysis

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Physical and Technological Parameters of Maize Varieties (Zea mays L.) Grown in the Southern Parts of Benin and the Influence of Chemical Composition

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Food and Nutrition Sciences 2016年第14期7卷 1386-1400页

作者： Josiane Adjobignon Semassa Victor Bienvenu Anihouvi Adolphe Adjanohoun Wilfrid Padonou Joachim Gbénou Lamine Baba-Moussa Laboratoire des Sciences des Aliments Faculté des Sciences Agronomiques Université d’Abomey-Calavi Cotonou Bénin Laboratoire de Biologie et de Typage Moléculaire en Microbiologie Département de Biochimie et de Biologie Cellulaire Université d’Abomey-Calavi Cotonou Bénin Centre de recherches agricoles Sud Institut National des Recherches Agricoles du Bénin Attogon Bénin Programme Technologie Agricole et Alimentaire Centre de Recherches Agricoles d’Agonkanmey Institut National des Recherches Agricoles du Bénin Abomey-Calavi Bénin Laboratoire de Pharmacognosie et des huiles essentielles Faculté des Sciences et Techniques Université d’Abomey-Calavi Cotonou Bénin

Objective: The study aims to investigate some physical and technological parameters of varieties of maize collected from the most representative areas of maize production in the southern part of Benin, and mainly used by the populations of those areas. Method: The chemical composition, physical and technological characteristics of the grains were determined using standard methods. Results: The results showed that the weight of 1000 grains ranges from 158.01 to 305.12 g, the length from 0.79 to 1.81 cm, the average width from 0.74 to 1.51 cm, thickness from 0.36 to 0.85 cm and density from 1.07 to 1.25. Regarding the technological parameters, the hardness of the grains before and after cooking was between 16.78 and 47.20 kgf, and 0.23 and 0.71 kgf respectively, while the cooking time varies from 71.00 and 158.33 mn. There was a positive and significant correlation (r = 0.405;p < 0.026) between the hardness after cooking and the weight of 1000 grains, between the hardness after cooking and grain width (r = 0.460;p < 0.011). A positive and highly significant correlation (r = 0.527, p < 0.003) was also observed between the hardness after cooking and the grain length, between the hardness after cooking and the thickness of grain (r = 0.476, p < 0.008), and between the grain density and the weight of 1000 grains (r = 0.481, p < 0.007). A positive and highly significant correlation (r = 0.923, p < 0.000) was also observed between maize grain length and width, and between grain length and grain thickness (r = 0.764, p < 0.000) and between the width and thickness (r = 0.764, p < 0.000). On the other hand, a significant negative correlation (r = -0.394, p < 0.031) was found between the hardness before cooking and the thickness and between the hardness before cooking and sphericity index (r = -0465*, p Conclusion: Through its chemical and technological characteristics the maize constitutes an important raw material for many culinary recipes in West African region.

关键词： Maize Physical Characteristics Technological Parameters Chemical Parameters

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Influence of Isolated PGPR Rhizobacteria in Central and Northern Benin on Maize Germination and Greenhouse Growth

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American Journal of Plant Sciences 2018年第13期9卷 2775-2793页

作者： Olaréwadjou Amogou Gustave Dagbénonbakin Nadege Adouke Agbodjato Pacome Agossou Noumavo Hafiz Adio Salami Salako Valere Aguegue Mevognon Ricardos Assogba Abado Sylvestre Koda Fousseni Abdel Djihal Adolphe Adjanohoun Lamine Baba-Moussa Departement de Biochimie et de Bio-logie Cellulaire Faculte des Sciences et Techniques(FAST)Laboratoire de Biologie et de Typage Moleculaire en MicrobiologieUniversite d’Abomey-Calavi(UAC)CotonouBénin Centre de recherches agricoles d’Agonkanmey Institut National des Recherches Agricoles du BéninCotonouBénin Département de Biologie Végétale Laboratoire de Microbiologie et des Technologies AlimentairesCotonouBénin Faculte des Science Agronomique(FSA) Laboratoire de Biomathématique et d’Estimation ForestièreUniversite d’Abomey-Calavi(UAC)CotonouBénin Centre de recherches agricoles Sud Institut National des Recherches Agricoles du BéninAttogonBénin

The objective of the study was to evaluate the effects of nine plant growth-promoting rhizobacteria (PGPR) alone or in combination on maize seed germination and seedling growth under laboratory and greenhouse conditions. The germination and growth tests were carried out in square petri dishes and pots. Maize seeds were inoculated with suspension of 108 CFU/ml of rhizobacteria. The experimental device was a random block of 16 treatments with four repetitions. Germination test results showed that seeds inoculated with PGPR including the control induced good germination in the range of 93.75% to 100%. The vigor index and root length of the seeds treated with Bacillus panthothenicus were significantly improved by 76.64% and 58.86%, respectively, while the maximal lengths of the seedlings were obtained with Pseudomonas cichorii with an increase of 118.95%. In greenhouse experience, data demonstrated that Serratia marcescens better improved the leaf area, height and underground biomass, respectively by 58.83%, 108.43%, and 59.16% as compared to the control. The highest fresh aerial biomass and air dry matter was obtained with plants treated only with Pseudomonas putida. These results show the potential to use such rhizobacteria as biofertilizers to improve maize productivity in Benin.

关键词： PGPR Biofertilization Germination Growth Soil Ferruginous

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Status of Techniques Used to Control Moulds in Maize Storage in Africa

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Agricultural Sciences 2022年第1期13卷 49-64页

作者： Halfane Lehmane Rafiatou Ba durand Dah-Nouvlessounon Haziz Sina Gautier Roko Farid T. Bade Akim Socohou Adolphe Adjanohoun Lamine Baba-Moussa Laboratory of Biology and Molecular Typing in Microbiology Department of Biochemistry and Cell Biology Faculty of Sciences and Techniques University of Abomey-Calavi Cotonou Benin institut national des recherches agricoles du Bénin Cotonou Benin

In Benin, the preservation of maize is a real problem involving important physical and chemical properties losses. Physical losses are due by rodents, pests and insects and chemical losses are mainly due to fungal infection. Several methods, products and practices are used by farmers to overcome these problems. The methods used do not always control the losses caused by fungal infection. The objective of the study was to make a bibliographic synthesis of the impacts induced by the items, practices and methods used to overcome the chemical losses of maize. In the search for information, the library of the Ministry of Agriculture, Livestock and Fisheries was consulted;the archives of the library of the University of Abomey-Calavi and the national institute of Agricultural Research of Benin were also consulted. Thus, chemicals such as sofagrain, Pyrimiphos-Methyl, Thiamethoxam, Pyrimiphos-Methyl, Permethrin, Deltamethrin, fumigant are used to control physical attacks on maize. Ash and plants species such as neem tree (Azadirachta indica) are generally used for the preservation and storage of maize seeds. In addition, chemicals such as arsenious anhydride and zinc phosphide are used for the preservation of corn. The use of these molecules and chemical products has negative impacts on human health and the environment. Of all the methods used, no treatment is still popularized for the reduction of the chemical losses caused by the fungal infection in corn storage.

关键词： Impact Aflatoxins Cereals Conservation Africa

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Generation of Synthetic Transcriptome Data with Defined Statistical Properties for the Development and Testing of New Analysis Methods

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Genomics, Proteomics & Bioinformatics 2007年第1期5卷 45-52页

作者： Guillaume Brysbaert Sebastian Noth Arndt Benecke Systems Epigenomics Group Institut des Hautes Etudes Scientifiques/Institut de Recherches InterdisciplinairesCNRS/INSERM 91440 Bures sur Yvette France

We have previously developed a combined signal/variance distribution model that accounts for the particular statistical properties of datasets generated on the Applied Biosystems AB1700 transcriptome system. Here we show that this model can be efficiently used to generate synthetic datasets with statistical properties virtually identical to those of the actual data by aid of the JAVA application *** creator 1.0 that we have developed. The fundamentally different structure of AB1700 transcriptome profiles requires re-evaluation, adaptation, or even redevelopment of many of the standard microarray analysis methods in order to avoid misinterpretation of the data on the one hand, and to draw full benefit from their increased specificity and sensitivity on the other hand. Our composite data model and the *** creator 1.0 application thereby not only present proof of the correctness of our parameter estimation, but also provide a tool for the generation of synthetic test data that will be useful for further development and testing of analysis methods.

关键词： transcriptome microarray analysis signal/variance distribution distribution modeling parameter approximation synthetic data generation

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人肺泡上皮细胞株A549和脐静脉内皮细胞中的内皮素-1通路(英文)

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Acta Pharmacologica Sinica 2000年第6期21卷 21-28页

作者： Isabelle DEPREZ-ROY,Francis COGE,Lydia BERTRY,Jean-Pierre GALIZZI,Michel FELETOU,Paul MVANHOUTTE,Emmanuel CANET institut de recherches Servier 11 rue des Moulineaux 92150 Suresnes France institut de recherches Servier 11 rue des Moulineaux 92150 Suresnes France institut de recherches Servier 11 rue des Moulineaux 92150 Suresnes France institut de recherches Servier 11 rue des Moulineaux 92150 Suresnes France institut de recherches Servier 11 rue des Moulineaux 92150 Suresnes France institut de recherches Servier 11 rue des Moulineaux 92150 Suresnes France institut de recherches Servier 11 rue des Moulineaux 92150 Suresnes France Departement de Dibetologie institut de recherches Servier II rue des Moulineaux 92150 Suresnes France.

AIM: This study was designed to characterize the en-dothelin pathway in an immortalized human adenocarcino-ma-derived alveolar epithelial cell line (A549) and human umbilical vein endothelial cell line (HUVEC). METHODS: The release of BT-1 and big-ET-1 was measured in the incubation medium of both cell lines. The expression of mRNAs coding for the endothelin iso-forms (hppET-1, -2, -3), the endothelin converting enzymes (hECE-la, b, c, and d) and the hETA and hETB receptors was investigated using RT-PCR. The expression of ECE-1 mRNA in various human tissues and in A549 cells was investigated by Northern blot analysis and the subcellular localization of ECE-1 in A549 cells was investigated by immunoblotting using a polyclonal antibody. RESULTS: Under control conditions, HUVEC release both ET-1 and big-ET-1 (ratio 5 to 1) while in A549 cells the big-ET-1 levels were below the threshold of detection. The release of these two peptides was minimally affected by various inhibitors of peptidases. However, in b

关键词：内皮素-1 分泌内皮素受体

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