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Natural Resources 2024年第3期15卷 61-68页

作者： Amadou Issoufou Abdourhimou Moussa Boubacar Habou Rabiou Soumana Idrissa Mahamane Ali Faculté des Sciences agronomiques Université de Diffa Diffa Niger institut national de la Recherche agronomique du Niger Niamey Niger Faculté des Sciences et Techniques Université Abdou Moumouni de Niamey Niamey Niger

Information on the dynamics of savannah is important to a country's plan to overcome the problems of uncontrolled development and environmental hazards. Taking the reserve partielle de Dosso, Niger as the case study area, this paper analyzed the long-term land use land cover change from 2002 to 2022. Satellite images were processed by using Google Earth Engine (GEE). Therefore, four major land cover classes were identified based on spectral characteristics of Land sat, namely, built-up, vegetation, cropland, bare land and water. The result revealed that barren and built-up areas increased at the expense of vegetation and water. From the four major land use land cover the large area is covered by vegetation which comprises about 192963.5 hectares followed by cropland and water consisting of 32506.43 and 1596.4 hectares respectively. The built-up area gained substantial area (most) during the study period. The reduction in some of the land cover/uses underlines the dangerous trend of the pressure poised by population growth and the changing functionality. Land cover change is influenced by a variety of societal factors operating on several spatial and temporal levels. The area estimates and spatial distributions of the LULC classes produced from the current study will assist local authorities, managers, and other stakeholders in decision-making and planning regarding forest land cover and uses.

关键词： Land Use/Cover Change detection Classification Dosso

Phenological stage effect on phenolic composition and repellent potential of Mentha pulegium against Tribolium castaneum and Lasioderma serricorne

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Asian Pacific Journal of Tropical Biomedicine 2018年第4期8卷 207-216页

作者： Nidhal Salem Jazia Sriti Olfa Bachrouch Kamel Msaada Saber Khammassi Majdi Hammami Saoussen Selmi Emna Boushih Marwa Ouertani Nesrine Hachani Manef Abderraba Brahim Marzouk Ferid Limam Jouda Mediouni Ben Jemaa Laboratoire des Substances Bioactives Centre de Biotechnologie a la Technopole de Borj Cedria Laboratoire de Protection des Vegetaux Institut National de la Recherche Agronomique de Tunisie (INRAT) institut Superieur des Sciences Biologiques Appliquees de Tunisia Laboratoire de Biotechnologie Appliquee a l'Agriculture Institut National de la Recherche Agronomique de Tunisie (INRAT)

Objective:To elucidate for the first time the phenological stage effect on polyphenol,flavonoid and proanthocyanidin contents of pennyroyal(Mentha pulegium)as well as their antioxidant capacities and insecticidal potentials against Tribolium castaneum and Lasioderma ***:Crude methanol extracts from pennyroyal(Mentha pulegium)aerial parts were evaluated for their anti radical,reducing power activities and repellent potentials against selected coleopteran insects during different phenological ***:Phenolic contents of pennyroyal aerial parts and their antioxidant effects were significantly dependent on maturity stage of the *** maximum of phenol production was reached during the full flowering *** was the major phenol in Mentha pulegium with an amount of6.01 mg/g of dry vegetable matter during this ***,at fructification stage,the highest antioxidant activity was not related to high phenolic *** repulsive effect of pennyroyal extract showed that Lasioderma serricorne was more susceptible to pennyroyal extract than Tribolium castarneum with corresponding median repellent dose values of 0.124and 0.006 mg/mL ***,great differences in insect repulsion depending on extract concentrations,exposure time and developmental stage was *** the lowest concentration(0.125 mg/mL),the repellent effect against Tribolium reached its maximum(90%)during the fructification stage(after 24 h of exposure)while this repellent effect was 80%during the vegetative ***:Due to the strong insecticidal potential of pennyroyal extracts,this study highlights the therapeutic properties of this plant and encourages its use as a safer,environmental-friendly and efficient insecticide in food industry.

关键词： Apigenin Lasioderma Repellent activity Full flowering stage

Epistasis and Maternal Effect in Resistance to Puccinia coronata ***. avenae Eriks in Oats(Avena sp.)

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Agricultural Sciences in China 2010年第10期9卷 1462-1466页

作者： Bnejdi F Hammami I Allagui M B Saadoun M el Gazzah M Laboratoire de Génétique et Biométrie Faculté des Sciences de Tunis Université Tunis institut national de la Recherche agronomique de tunisie(INRAT) Rue Hadi Karray

The objective of this paper was to investigate the mode of heredity for resistance in oats （Avena sp.） to crown rust caused by Puccinia coronata Cda. f. sp. avenae Eriks, Eight generations of 2 crosses were used to estimate genetic effects and narrow-sense heritability （NSH）. Separate generation means analysis （GMA） indicated a complex gene action controlling this trait with additive, dominance, epistatic and maternal effects （ME）. The genetic model which assumed no epistasis and no ME did not accurately describe the resistance to P. coronata. In both crosses, the digenic epistatic model with ME was sufficient to explain variation in generation means for isolate CRec58 and isolate CRec46. Additive dominance and epistatic components were negative in most cases, suggesting that gene effects contributed more to the resistance than to the susceptibility. The estimated values of NSH were 15-99% depending upon the cross and isolates. The results indicated that appropriate choice of maternal parent and recurrent selection would increase resistance to crown rust in oats.

关键词： epistasis heritability maternal effect resistance

Very high conservation between Cyp6a2 from Drosophila melanogaster and its ortholog Cyp6a26 from D. simulans

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Insect Science 2007年第1期14卷 15-27页

作者： SOPHIE TARES LAURY ARTHAUD ALEXANDRA BRUN-BARALE DIDIER CROCHARD JEAN-MARC BRIde MARCEL AMICHOT institut national de la Recherche agronomique UMR ROSE 1112 Sophia-Antipolis France

Although Drosophila simulans is closely related to D. melanogaster, very few cytochrome P450 genes have been studied in this species until now. As Cyp6a2 from D. melanogaster is a major gene implicated in the detoxification of xenobiotic molecules, we decided to look for its ortholog in D. simulans. The isolated gene, Cyp6a26, presents structural characteristics very similar to those of Cyp6a2： an identical size of 1590-bp comprising two exons separated by a 69-bp intron and a nucleotide sequence homology of 95%. Many putative transcriptionally important motifs were identified in the upstream DNAs of the two genes but only 16 elements are in common positions. Treatment of flies with phenobarbital leads to an increased production of Cyp6a26 mRNAs. The expression of Cyp6a26 mRNAs varies following developmental stages in the same manner as Cyp6a2. Immunohistochemistry experiments of phenobarbital-treated adult drosophila show that the spatial expression pattern of the two proteins is also conserved between the two species. All these data argue in favor of the conservation of the function of these homologous genes between the two Drosophila species.

关键词： cytochrome P450 Drosophila ortholog mRNA expression promotor

From Prototype to Inference:A Pipeline to Apply deep Learning in Sorghum Panicle detection

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Plant Phenomics 2023年第1期5卷 94-109页

作者： Chrisbin James Yanyang Gu Andries Potgieter Etienne David Simon Madec Wei Guo Frédéric Baret Anders Eriksson Scott Chapman School of Agriculture and Food Sciences The University of QueenslandBrisbaneAustralia School of Information Technology and Electrical Engineering The University of QueenslandBrisbaneAustralia Queensland Alliance for Agriculture and Food Innovation The University of QueenslandBrisbaneAustralia Arvalis Institut du VégétalParisFrance Graduate School of Agricultural and Life Sciences The University of TokyoTokyoJapan institut national de la Recherche agronomique ParisFrance

Head(panicle)density is a major component in understanding crop yield,especially in crops that produce variable numbers of tillers such as sorghum and *** of panicle density both in plant breeding and in the agronomy scouting of commercial crops typically relies on manual counts observation,which is an inefficient and tedious *** of the easy availability of red–green–blue images,machine learning approaches have been applied to replacing manual ***,much of this research focuses on detection per se in limited testing conditions and does not provide a general protocol to utilize deep-learning-based *** this paper,we provide a comprehensive pipeline from data collection to model deployment in deep-learning-assisted panicle yield estimation for *** pipeline provides a basis from data collection and model training,to model validation and model deployment in commercial *** model training is the foundation of the ***,in natural environments,the deployment dataset is frequently different from the training data(domain shift)causing the model to fail,so a robust model is essential to build a reliable *** we demonstrate our pipeline in a sorghum field,the pipeline can be generalized to other grain *** pipeline provides a high-resolution head density map that can be utilized for diagnosis of agronomic variability within a field,in a pipeline built without commercial software.

关键词： crops breeding utilize

Endophyte infection of Festuca eskia enhances seedling survival to drought and cutting at the expense of clonal expansion

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Journal of Plant Ecology 2011年第4期4卷 201-208页

作者： Anaïs Gibert Laurent Hazard institut national de la Recherche agronomique UMR 1248 AGIR31320 Castanet TolosanFrance

Symbiotic relationships between fungal endophytes and grass species are known to increase stress resistance in the grass host;however,there is little evidence to suggest that the positive effects occur early in the grass life *** this study,we explored the effects of the endophyte Epichloe¨festucae on the growth and survival of Festuca eskia seedlings under drought and frequent cutting *** Festuca eskia seedlings were collected from the western part of the plant repartition area in a non-symbiotic population located in a mesic and heavily grazed site(W-NS)and from the eastern part in a symbiotic population from a xeric and lightly grazed site(E-S).The E-S population was experimentally freed from its endophyte(E-F).Two greenhouse experiments were conducted to compare growth and survival between the three seedling types under drought stress and frequent *** the first experiment,126 seedlings per seedling type(n=378)were grown for 6 weeks under non-limiting conditions before the cessation of *** 3 weeks without irrigation,full irrigation was restored for 10 days to measure the survival *** length,leaf elongation rate and survival rate were assessed per *** the second experiment,156 seedlings per seedling type(n=468)were grown under non-limiting *** seedlings were cut to 3 cmhigh,twice a week,during the first month of *** elongation and tillering were monitored on 52 seedlings per seedling *** each type,seedling survival rate was determined by the number of plants alive after 10 days of regrowth,without *** Findings The drought experiment revealed a phenotypic differentiation to drought in 30 *** populations,suggesting adaptive differentiation:the eastern seedlings showed the highest survival rate.A tradeoff between growth and survival was highlighted:the highest drought survival rate was associated with the lowest leaf elongation rate under non-limiting water ***

关键词： Epichloëfestucae water stress cutting fitness native grass mutualism

Multichannel imaging for monitoring chemical composition and germination capacity of cowpea(Vigna unguiculata) seeds during development and maturation

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The Crop Journal 2022年第5期10卷 1399-1411页

作者： Gamal ElMasry Nasser Mandour Yahya Ejeez Didier demilly Salim Al-Rejaie Jerome Verdier Etienne Belin David Rousseau Agricultural Engineering department Faculty of AgricultureSuez Canal UniversityIsmailiaEgypt Groupe d’Etude et de Controle des Variétés et des Semences(GEVES) Station Nationale d’Essais de Semences(SNES)Beaucouze49071AngersFrance department of Pharmacology&Toxicology College of PharmacyKing Saud UniversitySaudi Arabia Laboratoire Angevin de Recherche en Ingenierie des Systemes(LARIS) Universited’AngersAngersFrance institut national de la Recherche agronomique(INRA) UMR1345 Institut de Recherche en Horticulture et SemencesBeaucouzéF-49071AngersFrance

This study aimed to set a computer-integrated multichannel spectral imaging system as a high-throughput phenotyping tool for the analysis of individual cowpea seeds harvested at different developmental stages. The changes in germination capacity and variations in moisture, protein and different sugars during twelve stages of seed development from 10 to 32 days after anthesis were nondestructively monitored. Multispectral data at 20 discrete wavelengths in the ultraviolet, visible and near infrared regions were extracted from individual seeds and then modelled using partial least squares regression and linear discriminant analysis(LDA) models. The developed multivariate models were accurate enough for monitoring all possible changes occurred in moisture, protein and sugar contents with coefficients of determination in prediction R^(2) of 0.93, 0.80 and 0.78 and root mean square errors in prediction(RMSEP) of 6.045%, 2.236% and 0.890%, respectively. The accuracy of PLS models in predicting individual sugars such as verbascose and stachyose was reasonable with R~2 of 0.87 and 0.87 and RMSEP of 0.071%and 0.485%, respectively;but for the prediction of sucrose and raffinose the accuracy was relatively limited with R^(2) of 0.24 and 0.66 and RMSEP of 0.567% and 0.045%, respectively. The developed LDA model was robust in classifying the seeds based on their germination capacity with overall correct classification of96.33% and 95.67% in the training and validation datasets, respectively. With these levels of accuracy,the proposed multichannel spectral imaging system designed for single seeds could be an effective choice as a rapid screening and non-destructive technique for identifying the ideal harvesting time of cowpea seeds based on their chemical composition and germination capacity. Moreover, the development of chemical images of the major constituents along with classification images confirmed the usefulness of the proposed technique as a non-destructive tool for estim

关键词： Multispectral imaging Multichannel imaging Chemical imaging Spectral analysis Seeds Cowpea

Dimensions of hepatocellular carcinoma phenotypic diversity

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World Journal of Gastroenterology 2018年第40期24卷 4536-4547页

作者： Romain Désert Natalia Nieto Orlando Musso institut NuMeCan Université de Rennes 1 Institut national de la recherche agronomique (INRA) Institut national de la santé et de la recherche médicale (INSERM) department of Pathology Department of Medicine (Gastroenterology and Hepatology) University of Illinois at Chicago

Hepatocellular carcinoma(HCC) is the 3^(rd) leading cause of cancer-related death worldwide. More than 80% of HCCs arise within chronic liver disease resulting from viral hepatitis, alcohol, hemochromatosis, obesity and metabolic syndrome or genotoxins. Projections based on Western lifestyle and its metabolic consequences anticipate a further increase in incidence, despite recent breakthroughs in the management of viral hepatitis. HCCs display high heterogeneity of molecular phenotypes, which challenges clinical management. However, emerging molecular classifications of HCCs have not yet formed a unified corpus translatable to the clinical practice. Thus, patient management is currently based upon tumor number, size, vascular invasion, performance status and functional liver reserve. Nonetheless, an impressive body of molecular evidence emerged within the last 20 years and is becoming increasingly available to medical practitioners and researchers in the form of repositories. Therefore, the aim this work is to review molecular data underlying HCC classifications and to organize this corpus into the major dimensions explaining HCC phenotypic diversity. Major efforts have been recently made worldwide toward a unifying "clinically-friendly" molecular landscape. As a result, a consensus emerges on three major dimensions explaining the HCC heterogeneity. In the first dimension, tumor cell proliferation and differentiation enabled allocation of HCCs to two major classes presenting profoundly different clinical aggressiveness. In the second dimension, HCC microenvironment and tumor immunity underlie recent therapeutic breakthroughs prolonging patients' survival. In the third dimension,metabolic reprogramming, with the recent emergence of subclass-specific metabolic profiles, may lead to adaptive and combined therapeutic approaches. Therefore, here we review recent molecular evidence, their impact on tumor histopathological features and clinical behavior and highlight the r

关键词： Liver metabolism Liver zonation Hepatocellular carcinoma classification Wnt/β-catenin TP53 Tumor microenvironment Inflammation Tumor immunity Hepatocyte proliferation Hepatocyte differentiation

Oil and fatty acid accumulation during coriander (Coriandrum sativum L.) fruit ripening under organic cultivation

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The Crop Journal 2015年第4期3卷 366-369页

作者： Quang-Hung Nguyen Thierry Talou Mureil Cerny Philippe Evon Othmane Merah Université Fédérale de Toulouse Midi-Pyrénées INP-ENSIACET Laboratoire de Chimie Agro-industrielle institut national de Recherche agronomique UMR 1010 CAI

To evaluate the accumulation of oil and fatty acids in coriander during fruit ripening, a field experiment was conducted under organic cultivation conditions in Auch(near Toulouse,southwestern France) during the 2009 cropping season. The percentage and composition of the fatty acids of coriander were determined by gas chromatography. Our results showed that rapid oil accumulation started in early stages(two days after flowering, DAF). Twelve fatty acids were identified. Saturated and polyunsaturated acids were the dominant fatty acids at earlier stages(2–12 DAF), but decreased after this date. After this stage,petroselinic acid increased to its highest amount at 18 DAF. In contrast, palmitic acid followed the opposite trend. Saturated and polyunsaturated fatty acids decreased markedly and monounsaturated fatty acids increased during fruit maturation. It appears that the fruit of coriander may be harvested before full maturity.

关键词： Oil content Petroselinic acid Coriander Organic cultivation Fatty acid accumulation

Farmer Participatory Evaluation of Sorghum Hybrid in Niger

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Journal of Agricultural Chemistry and Environment 2023年第4期12卷 386-396页

作者： Mamadou Ibrahim Aissata Hamé Abdou Kadi Kadi Abdelkader Mahaman Soulé Zakari Moussa Ousmane institut national de la Recherche agronomique du Niger (INRAN) Niamey Niger Faculté d’Agronomie Université Abdou Moumouni (UAM) Niamey Niger

Participatory varietal selection (PVS) with farmers and Seed Companies was conducted at Maradi research station (07°05'E/13°48'N) and in farmer field to evaluate and select sorghum hybrid varieties for high yield and other important agronomic traits. The experimental design was a randomized complete block with three replications where flowering (days), plant height (cm) and yield (kg) were collected. The analysis of variance showed highly significance among treatments of all traits measured Environment and Genotype by Environment interactions also contributed significantly to the performance of yield components. The highest average grain yield was recorded from hybrids P9511A x ST9007-5-2-1 (4289 kg/ha), NE223A x 90SN1 (3666 kg/ha), NE223A x Sepon 82 (3533 kg/ha) and NE223A x P9405 (3519 kg/ha) across locations. Farmers’ preferences were the panicle size, good seed set, earliness, and seed color. Hence, in a variety selection farmer’s preferences focus more on prioritized yield-related trait. The best varieties ranked by traits of interest were P9511A x ST9007-5-2-1, NE223A x 90SN1, NE223A x P9405 and P9511A x SEPON 82 that performed well under their circumstances. The results showed that farmers’ preferred varieties match with researchers. Therefore, based on objectively measured traits, farmers’ preferences and the agro ecologies of the site, varieties NE223A x 90SN1 and P9511A x ST9007-5-2-1 were found promising for production.

关键词： Sorghum Breeding Multi-Location Testing Participatory Approaches Yield Niger