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P450 gene CYP321A8 is responsible for cross-resistance of insecticides in field populations of Spodoptera frugiperda

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insect Science 2025年第1期32卷 227-242页

作者： Yumei Chen Yongjie Cen Yu Liu Yanan Peng Yiguang Lin Qili Feng Yong Xiao Sichun Zheng Guangdong Provincial Key Laboratory of insect Developmental Biology and Applied Technology Guangzhou Key Laboratory of Insect Development Regulation and Application ResearchInstitute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina Institute of Plant Protection Guangdong Academy of Agricultural SciencesKey Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural AffairsGuangdong Provincial Key Laboratory of High Technology for Plant ProtectionGuangzhouChina

Continuous and long-term use of traditional and new pesticides can result in cross-resistance among pest populations in different *** on the mechanism of cross-resistance and related genes will help resistance management and field pest *** this study,the pesticide-resistance mechanism in Spodoptera frugiperda(FAW)was studied with field populations in 3 locations of South *** FAW populations were highly resistant to traditional insecticides,chlorpyrifos(organophosphate)and deltamethrin(pyrethroid),and had higher levels of cytochrome P450 activity than a non-resistant laboratory *** of P450 activity by piperonyl butoxide significantly increased the sensitivity of resistant FAW in 3 locations to chlorpyrifos,deltamethrin and chlorantraniliprole(amide),a new type of insecticide,suggesting that P450 detoxification is a critical factor for insecticide resistance in field FAW *** analysis indicated that 18 P450 genes were upregulated in the field FAW populations collected in 3 regions and in 2 consecutive years,with CYP321A8,the most significantly upregulated *** of CYP321A8 messenger RNA by RNA interference resulted in an increased sensitivity to the 3 tested insecticides in the field *** activity and molecular docking analyses indicated that CYP321A8 enzyme was able to metabolize the 3 tested insecticides and interact with 8 other types of insecticides,confirming that CYP321A8 is a key cross-resistance gene with a wide range of substrates in the field FAW populations across the different regions and can be used as a biomarker and target for management of FAW insecticide resistance in fields.

关键词： cross-resistance cytochrome P450 field population insecticide resistance Spodopterafrugiperda

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Optimizing methyl-eugenol aromatherapy to maximize posttreatment effects to enhance mating competitiveness of male Bactrocera carambolae （Diptera： Tephritidae）

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insect Science 2015年第5期22卷 661-669页

作者： Ihsan ul Haq Marc J.B. Vreysen Carlos Caceres Todd E. Shelly Jorge Hendrichs insect pest control Laboratory Joint FAOIIAEA Division of Nuclear Techniques in Food and Agriculture Seibersdorf Austria National Agricultural Research Centre Park Road Islamabad Pakistan USDAIAPHIS 41-650 Ahiki Street Waimanalo HI 96795 USA and insect pest control section Joint FAOIIAEA Division of Nuclear Techniques in Food and Agriculture Vienna Austria

Methyl-eugenol （ME）（1,2-dimethoxy-4-（2-propenyl）benzene）, a natural phy- tochemical, did enhance male Bactrocera carambolae Drew ＆ Hancock （Diptera： Tephri- tidae） mating competitiveness 3 d after ingestion. Enhanced male mating competitiveness can significantly increase the effectiveness of the sterile insect technique （SIT）. ME appli- cation to mass reared sterile flies by feeding is infeasible. ME application by aromatherapy however, would be a very practical way of ME application in fly emergence and release facilities. This approach was shown to enhance mating competitiveness ofB. carambolae 3 d posttreatment （DPT）. Despite this added benefit, every additional day of delaying re- lease will reduce sterile fly quality and will add cost to SIT application. The present study was planned to assess the effects of ME-aromatherapy on male B. carambolae mating competitiveness 1DPT and 2DPT. ME aromatherapy 1DPT or 2DPT did enhance mating competitiveness of B. carambolae males whereas ME feeding 1DPT and 2DPT did not. Male mating competitiveness was enhanced by the ME aromatherapy irrespective if they received 1DPT, 2DPT or 3DPT. ME aromatherapy, being a viable approach for its applica- tion, did enhance mating competitiveness of male B. carambolae 1 d posttreatrnent as ME feeding did 3 d after ingestion.

关键词： Bactrocera carambolae mating competitiveness ME aromatherapy methyl-eugenol ME feeding posttreatment effect SIT

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Genome-wide identification and characterization of putative effectors in Heterodera schachtii that suppress plant immune response

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Journal of Integrative Agriculture 2025年第1期24卷 196-208页

作者： Ke Yao Menghan Zhang Jianjun Xu Deliang Peng Wenkun Huang Ling'an Kong Shiming Liu Guangkuo Li Huan Peng State Key Laboratory for Biology of Plant Diseases and insect pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Key Laboratory of Integrated pest Management on Crop in Northwestern Oasis Institute of Plant ProtectionXinjiang Academy of Agricultural Sciences/Scientific Observing and Experimental Station of KorlaMinistry of Agriculture and Rural AffairsUrumqi 830013China Zhongyuan Research Center Chinese Academy of Agricultural SciencesXinxiang 453000China

The sugar beet cyst nematode(Heterodera schachtii) is one of the most destructive pathogens in sugar beet production, which causes serious economic losses every year. Few molecular details of effectors of H. schachtii parasitism are known. We analyzed the genome and transcriptome data of H. schachtii and identified multiple potential predicted proteins. After filtering out predicted proteins with high homology to other plant-parasitic nematodes, we performed functional validation of the remaining effector proteins. 37 putative effectors of H. schachtii were screened based on the Nicotiana benthamiana system for identifying the effectors that inhibit plant immune response, eventually determines 13 candidate effectors could inhibit cell death caused by Bax. Among the 13 effectors, nine have the ability to inhibit GPA2/RBP1-induced cell death. All 13 effectortriggered immunity(ETI) suppressor genes were analyzed by qRT-PCR and confirmed to result in a significant downregulation of one or more defense genes during infection compared to empty vector. For in situ hybridization,13 effectors were specifically expressed and located in esophageal gland cells. These data and functional analysis set the stage for further studies on the interaction of H. schachtii with host and H. schachtii parasitic control.

关键词： sugar beet cyst nematode Heterodera schachti effector-triggered immunity hypersensitive response effector

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Methoprene application and diet protein supplementation to male melon fly, Bactrocera cucurbitae, modifies female remating behavior

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insect Science 2014年第5期21卷 637-646页

作者： Ihsan ul Haq Marc J. B. Vreysen P. E. A. Teal Jorge Hendrichs insect pest control Laboratory Joint FAO/IAEA Agriculture and Biotechnology Laboratories A-2444 Seibersdorf Austria NationalAgricultural Research Centre Park Road Islamabad 45500 Pakistan Center for Medical Agricultural and Veterinary EntomologyUSDA ARS Gainesville Florida 32604 USA insect pest control section Joint FAO/IAEA Division IAEA A-1400 Vienna Austria

Methoprene （an analogue of juvenile hormone） application and feeding on a protein diet is known to enhance male melon fly, Bactrocera cucurbitae Coquillett （Diptera： Tephritidae）, mating success. In this study, we investigated the effect of these treatments on male B. cucurbitae＇s ability to inhibit female remating. While 14-d-old females were fed on protein diet, 6-d-old males were exposed to one of the following treatments：（i） topical application of methoprene and fed on a protein diet; （ii） no methoprene but fed on a protein diet; （iii） methoprene and sugar-fed only; and （iv） sugar-fed, 14-d-old males acted as controls. Treatments had no effect on a male＇s ability to depress the female remating receptivity in comparison to the control. Females mated with protein-deprived males showed higher remating receptivity than females first mated with protein-fed males. Methoprene and protein diet interaction had a positive effect on male mating success during the first and second mating of females. Significantly more females first mated with sugar-fed males remated with protein-fed males and females first mated with methoprene treated and protein-fed males were more likely to remate with similarly treated males. Females mating latency （time to start mating） was significantly shorter with protein-fed males, and mating duration was significantly longer with protein-fed males compared with protein-deprived males. These results are discussed in the context of methoprene and/or dietary protein as prerelease treatment of sterile males in area-wide control of melon fly integrating the sterile insect technique （SIT）.

关键词： Bactrocera cucurbitae methoprene protein rich diet remating behavior SIT Tephritidae

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CRISPR-mediated mutagenesis of the odorant receptor co-receptor(Orco)gene disrupts olfaction-mediated behaviors in Bactrocera dorsalis

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insect Science 2022年第5期29卷 1275-1286页

作者： Li Xu Hong-Bo Jiang Kai-Yue Tang Ying Yan Marc F.Schetelig Jin-Jun Wang Key Laboratory of Entomology and pest control Engineering College of Plant ProtectionSouthwest UniversityChongqingChina International Joint Laboratory of China-Belgium on Sustainable Crop pest control Academy of Agricultural SciencesSouthwest UniversityChongqingChina Department of insect Biotechnology in Plant Protection Justus-Liebig-University GiessenInstitute for Insect BiotechnologyGiessenGermany

Olfaction plays an essential role in insect behavior such as host location,foraging,mating,and *** odorant receptor co-receptor(Orco)is an obligatory odorant receptor and indispensable in odor ***,we characterized the Orco gene from the oriental fruit fly,Bactrocera dorsalis(Hendel),a notorious agriculture *** olfactory deficiency mutants were generated by editing the BdorOrco gene using the CRISPR/Cas9 ***(EAG)and olfactory preference assays confirmed that BdorOrco^(−/−)mutant flies had reduced perception of methyl eugenol,β-caryophyllene,and ethyl *** bioassays showed that the eggs laid by BdorOrco^(−/−)females mediated by benzothiazole and 1-octen-3-ol were significantly *** addition,BdorOrco^(−/−)mutant flies took a significantly longer time to locate the food source compared with wild type(WT)***,our data indicated that Orco is essential for multiple physiological processes in ***,and it expands our understanding of the function of insect Orco.

关键词： CRISPR/Cas9 foraging olfaction oriental fruit fly oviposition

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Identification and expression profiles of gustatory receptor genes in Bactrocera minax larvae(Diptera:Tephritidae):Role of BminGR59f in larval growth

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insect Science 2022年第5期29卷 1240-1250页

作者： Guijian Zhang Shuai Cao Tong Guo Haoran Wang Xuewei Qi Xueming Ren Changying Niu Hubei Key Laboratory of insect Resource Application and Sustainable pest control College of Plant Science&TechnologyHuazhong Agricultural UniversityWuhanChina

insects employ various types of gustatory receptors(GRs)to identify nutrient-rich food and avoid toxic *** larval gustatory system is the critical checkpoint for food acceptance or *** a specialist herbivore,the larvae of Bactrocera minax feed only on unripe citrus ***,how larvae use GRs to check and adapt to the secondary metabolites in unripe citrus fruits remains *** this study,we first performed developmental expression profiles showing that most BminGRs genes were highly expressed in 1st and 2nd instar larvae and that tissue-specific expression indicated high expression of most BminGRs genes in the mouthparts of 2nd instar ***,we found that silencing BminGR59f by RNA interference(RNAi)affected the growth of 2nd instar *** *** and naringin were screened as ligands of BminGR59f via RNAi and cell calcium imaging,and the combination of these two flavones increased the body weight of *** summary,we identified a novel gustatory perception pattern in *** for detecting hesperidin and naringin,which boosted the growth of *** *** results shed light on how specialist herbivores detect and adapt to host metbolites in adverse environments depending on larval GRs.

关键词： gustatory receptors secondary metabolites specialist herbivores tephritid unripe citrus fruits

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Tradeoff between triglyceride consumption and ovariole development in Plutella xylostella(L.) released in mixed-host environments

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Journal of Integrative Agriculture 2019年第4期18卷 865-872页

作者： HUANG Bin SHI Zhang-hong HOU You-ming State Key Laboratory of Ecological pest control for Fujian and Taiwan Crops/Key Laboratory of insect Ecology in Fujian/College of Plant Protection Fujian Agriculture and Forestry University

After exposure to mixed environments with multiple hosts, the frequency of flight take-offs for Plutella xylostella(L.) is known to increase with a concomitant decrease in female fecundity. The objective of the present study was to elucidate the physiological mechanism underlying the increased flight activity and decreased fecundity of P. xylostella in mixed-host environments. We measured ovariole length, and triglyceride and vitellogenin(Vg) content in P. xylostella females after they were released into different host environments consisting of either a single host(Brassica campestris, Brassica oleracea or Brassica juncea) or a mixture of all three hosts. Our results indicated that ovariole length varied significantly between female adults from different host environments. Females from the B. campestris environment had the longest ovarioles, whereas those from the mixed environment had the shortest ones. A negative correlation was found between ovariole length and the flight take-off frequency of P. xylostella adults. Additionally, there were significant differences in the triglyceride content of P. xylostella females from different host environments. Our data revealed that more triglyceride was consumed by P. xylostella female adults from B. oleracea and the mixed environments than those from environments containing only B. campestris or B. juncea. In contrast, the relative Vg content in P. xylostella females from the mixed environment was lower than that in females from the B. campestris-, B. juncea-or B. oleracea-only environments. In conclusion, the mixed environment caused increased consumption of available energy resources(triglyceride) at the cost of retarding ovarian development and decreasing the amount of Vg produced.

关键词： diamondback moth triglyceride Lepidoptera ovariole length Plutellidae vitellogenin

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Patterns of biomass,carbon,and nitrogen storage distribution dynamics after the invasion of pine forests by Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae) in the three Gorges Reservoir Region

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Journal of Forestry Research 2018年第2期29卷 453-464页

作者： Ruihe Gao Youqing Luo Zhuang Wang Hanjun Yu Juan Shi Beijing Key Laboratory for Forest pest control Beijing Forestry University

Masson pine stands infected by Pine wilt disease(PWD) in the Three Gorges Reservoir Region of central China were surveyed to quantify the immediate responses and subsequent trajectories of biomass,carbon(C),and nitrogen(N) in stand-level major ecosystem *** biomasses of above-and belowground tree components,as well as of the understory,forest floor,and mineral soil(0–40 cm),were determined within each stand.C and N storage were also estimated for each ecosystem *** biomass decreased steadily with the extent of PWD *** biomass ranged from1.97 to 4.16 Mg ha,and the observed value for forest floor biomass was 12.89–22.59 Mg *** highest mean C and N concentrations were found in the stem bark and needles of Masson pine,respectively,while the lowest were found in the semi-to fully decomposed layer of the forest floor and stem wood of Masson pine,*** C and N storage of aboveground trees,tree roots,and the aboveground ecosystem decreased with the extent ofPWD ***,the C and N contents of the understory,forest floor,and total mineral soil initially declined after PWD infection before recovering over the following several *** result concluded that the biomass,C,and N storage of different forest ecosystem compartments have experienced certain variations following the PWD *** is vital to understand the shifts in stand-level C and N allocation in PWD-damaged forest stands,as well as for predicting the responses of regional and global C and N cycling.

关键词： Carbon insect outbreak Masson pine Nitrogen Pine sawyer beetle Pine wilt disease

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Isolation and characterization of insecticidal activity of （Z）- asarone from Acorus calamus L.

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insect Science 2008年第3期15卷 229-236页

作者： Yingjuan Yao Wanlun Cai Changju Yang Dong Xue Yanzhang Huang Institute of Urban pest control Huazhong Agriculture University Wuhan China

The insecticidal activity of Acorus calamus L. rhizome-derived material against adults of Sitophilus zeamais Motschulsky was examined by using repellency method and contact toxicity. The biologically active constituent of the A. calamus rhizome was separated and identified. The results showed that the ethanol extract of A. calamus had strong repellency and contact effect to S. zeamais and the active constituent of the A. calamus was characterized as （Z）-asarone by spectroscopic analysis. Responses from the tests varied with exposure times and doses. In the repellency test, ethanol extract of A. calamus had 93.92% repellency at 629.08 μg/cm^2 but only 71.38% at 157.27 μg/cm^2 12 h after treatment. As a contrast, （Z）-asarone showed 84.50% repellency at 314.54μg/cm^2 and 77.02% at 78.63 μg/cm^2 12 h after treatment. In the filter paper diffusion test, ethanol extract of A. calamus caused 95.56% and 17.78% mortality to S. zeamais at 314.54 μg/cm^2 and 78.63 μg/ cm^2 4 days after treatment, while （Z）-asarone brought about 100.00% and 15.56% mortality at 40.89 μg/cm^2 and 15.73 μg/cm^2 respectively. These results indicate that the insecticidal activity of the A. calamus extract may be due to （Z）-asarone.

关键词： Acorus calamus asarone natural insecticide Sitophilus zeamais

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Wing patterning genes of Nilaparvata lugens identification by transcriptome analysis, and their differential expression profile in wing pads between brachypterous and macropterous morphs

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Journal of Integrative Agriculture 2015年第9期14卷 1796-1807页

作者： LI Kai-yin HU Ding-bang LIU Fang-zhou LONG Man LIU Si-yi ZHAO Jing HE Yue-ping HUA Hong-xia Hubei insect Resources Utilization and Sustainable pest Management Key Laboratory College of Plant Science and TechnologyHuazhong Agricultural University

The brown planthopper, Nilaparvata lugens is an economically important pest on rice plants. This species produces macropterous and brachypterous morphs in response to environmental cues, which makes it very dififcult to control. The molecular basis of wing patterning in N. lugens is stil unknown. It is necessary to identify wing patterning genes of N. lugens, and also to clarify the expression differences of wing patterning genes between macropterous and brachypter-ous morphs. High-throughput deep sequencing of transcriptome of N. lugens wing pad yielded 116 744 580 raw reads and 113 042 700 clean reads. Al the reads were assembled into 55 963 unigenes with an average length of 804 bp. With the E-value cut-off of 1.0E–5,18 359 and 2 883 unigens had hits in NCBI-NR (NCBI non-redundant protein sequences) and NCBI-NT (NCBI nucleotide sequences) databases, respectively. A total of 16 502 unigenes were assigned to GO (gene ontology) classiifcation, 9 709 ungenes were grouped into 26 COG (cluster of orthologous groups of proteins) classiifcations, and 6 724 unigenes were assigned to different KEGG (Kyoto encyclopedia of genes and genomes) path-ways. In total, 56 unigenes which are homologous to wing patterning genes of Drosophila melanogaster or Tribolium castaneum were identiifed. Out of the 56 unigenes, 24 unigenes were selected, and their expression levels across the ifve nymphal stages between macropterous strain and brachypterous strain were examined by qRT-PCR. Two-way ANOVA analysis showed that development stage had signiifcant effects on the expression level of al the 24 genes (Pinsect, N. lugens. The identiifed wing patterning genes would be useful resource for future exploration of molecular basis of wing development. The 8 differential y expressed win

关键词： Nilaparvata lugens wing pad transcriptome wing patterning genes

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