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HT-SuperSAGE of the gut tissue of a Vip3Aa-resistant Heliothis virescens (Lepidoptera: Noctuidae) strain provides insights into the basis of resistance

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insect Science 2019年第3期26卷 479-498页

作者： Camilo Ayra-Pardo Maria E. Ochagavia Ben Raymond Asim Gulzar Lianet Rodriguez-Cabrera Claudia Rodriguez de la Noval Ivis Moran Bertot Ryohei Terauchi Kentaro Yoshida Hideo Matsumura Pilar Tellez Rodriguez Daily Hernandez Hemandez Orlando Borras-Hidalgo Denis J. Wright Plant Division Centre for Genetic Engineering and Biotechnology (CIGB) Havana Cuba Department of Life Sciences Faculty of Natural Sciences Imperial College London Berkshire UK Genetics and Genomics Research Group Iwate Biotechnology Research Center Kitakami Japan Current addresses: University of Exeter Penryn campus Penryn Cornwall TR10 9FE UK Department of Entomology University of Arid Agriculture Rawalpindi Muree Road Rawalpindi 46300 Pakistan laboratory of Plant Genetics Kobe University 1-1 Rokkodai-cho Nada ward Kobe 657-8501 Japan

Multitoxin Bt-crops expressing insecticidal toxins with different modes of action, for example, Cry and Vip, are expected to improve resistance management in target pests. While Cry1A resistance has been relatively well characterized in some insect species, this is not the case for Vip3A, for which no mechanism of resistance has yet been identified. Here we applied HT-SuperSAGE to analyze the transcriptome of the gut tissue of tobacco budworm Heliothis virescens (F.) laboratory-selected for Vip3Aa resistance. From a total of 1 324 252 sequence reads, 5 895 126-bp tags were obtained representing 17 751 nonsingleton unique transcripts (UniTags) from genetically similar Vip3Aa-resistant (Vip- Sel) and susceptible control (Vip-Unsel) strains. Differential expression was significant (≥2.5 fold or ≤0.4;P < 0.05) for 1989 sequences (11.2% of total UniTags), where 420 represented overexpressed (OE) and 1569 underexpressed (UE) genes in Vip-Sel. BLASTN searches mapped 419 UniTags to H. virescens sequence contigs, of which, 416 (106 OE and 310 UE) were unambiguously annotated to proteins in NCBI nonredundant protein databases. Gene Ontology distributed 345 of annotated UniTags in 14 functional categories with metabolism (including serine-type hydrolases) and translation/ribosome biogenesis being the most prevalent. A UniTag homologous to a particular member of the REsponse to PAThogen (REPAT) family was found among most overexpressed, while UniTags related to the putative Vip3Aa-binding ribosomal protein S2 (RpS2) were underexpressed. qRT-PCR of a subset of UniTags validated the HT-SuperSAGE data. This study is the first providing lepidopteran gut transcriptome associated with Vip3Aa resistance and a foundation for future attempts to elucidate the resistance mechanism.

关键词： Bt resistance Heliothis virescens HT-SuperSAGE transcriptomics Vip3Aa

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Integration of body-mounted ultrasoft organic solar cell on cyborg insects with intact mobility

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npj Flexible Electronics 2022年第1期6卷 726-734页

作者： Yujiro Kakei Shumpei Katayama Shinyoung Lee Masahito Takakuwa Kazuya Furusawa Shinjiro Umezu Hirotaka Sato Kenjiro Fukuda Takao Someya Center for Emergent Matter Science RIKEN2-1 HirosawaWakoSaitama 351-0198Japan Department of Integrative Bioscience and Biomedical Engineering Center for Advanced Biomedical SciencesTWIns Waseda Univerisity2-2 Wakamatsu-choShinjuku-kuTokyo 162-8480Japan Department of Modern Mechanical Engineering Waseda University3-4-1 OkuboShinjuku-kuTokyo 169-8555Japan Department of Applied Chemistry and Food Science Faculty of Environmental and Information SciencesFukui University of Technology3-6-1 GakuenFukui 910-8505Japan School of Mechanical and Aerospace Engineering Nanyang Technological UniversityN3.2-01-2065 Nanyang DriveSingapore 637460Singapore Thin-Film Device laboratory RIKEN2-1 HirosawaWakoSaitama 351-0198Japan Electrical and Electronic Engineering and Information Systems The University of Tokyo7-3-1 HongoBunkyo-kuTokyo 113-8656Japan

Cyborg insects have been proposed for applications such as urban search and ***-mounted energy-harvesting devices are critical for expanding the range of activity and functionality of cyborg ***,their power outputs are limited to less than 1 mW,which is considerably lower than those required for wireless locomotion *** area and load of the energy harvesting device considerably impair the mobility of tiny ***,we describe the integration of an ultrasoft organic solar cell module on cyborg insects that preserves their motion *** quantified system design strategy,developed using a combination of ultrathin film electronics and an adhesive-nonadhesive interleaving structure to perform basic insect motion,successfully achieved the fundamental locomotion of traversing and *** body-mounted ultrathin organic solar cell module achieves a power output of 17.2 *** demonstrate its feasibility by displaying the recharging wireless locomotion control of cyborg insects.

关键词： mounted harvesting considerably

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Occurrence and control of Main Soybean Diseases and insect pests in Greater Mekong Sub-region

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Agricultural Biotechnology 2018年第3期7卷 60-62,136页

作者： Qiong LI Hongbin LYU Lu FENG Mingliang DING Xiaoming ZHANG Institute of Food Crops Yunnan Academy of Agricultural Sciences Department of International Cooperation Yunnan Academy of Agricultural Sciences College of Plant Protection Yunnan Agricultural University/National Key Laboratory for the Protection and Utilization of Biological Resources in Yunnan

Soybean rust,soybean downy mildew,and soybean thrips,soybean pod borers,and soybean nocturnal moths are the world wide diseases and insect pests in soybean production,which pose a potential threat to soybean production in Great Mekong Sub-region（ GMS）,comprising Cambodia,Lao People＇s Democratic Republic,Myanmar,Thailand,Vietnam,and Yunnan province,the People＇s Republic of China. This paper summarized the host range,epidemiology,damage and control methods of these diseases and insect pests in GMS,with the aim to provide information basis for understanding and effective control of soybean diseases and insect pests in GMS.

关键词： Soybean Disease insect pest Occurrence control GMS

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Biological Aspects of Orius similis Zheng Reared on Two Preys at Three Constant Temperatures

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Journal of Agricultural Science and Technology(A) 2018年第6期8卷 350-363页

作者： Marwa Elsayed Sanad Amer Yueguan Fu Liming Niu Department of Integrated pest Management Environmental and Plant Protection Institute(EPPI)Chinese Academy of Tropical Agricultural ScienceHaikou CityHainan Province 571101China Department of Scale insects and Mealybugs Plant Protection Research Institute(PPRI)Agricultural Research Center(ARC)DokkiGiza 12611Egypt

Mass-rearing of Orius similis Zheng on two preys,Aphis craccivora Koch and eggs of Corcyra cephalonica Stainton at three constant temperatures(22,26 and 30°C)and 60%±10%RH and 16:8 L/D photoperiod under laboratory conditions was investigated to study the effect of different temperatures and different preys on the biology of *** highest survival rate(%)of nymphal stages was 81.14%and the longest oviposition period for females(20.6 d)was recorded at 26°***,the highest fecundity of female also recorded at 26°*** highest rate of nymphal feeding consumption was(122.5 individuals of ***)also,recorded at 26°*** well as,the two preys had significant effects on the biological characteristics of *** highest survival rate for nymphal stage was recorded when *** nymphs fed on ***,the longest survival rate was founded in the nymphs which fed on the individuals of *** the nymphal period of *** which consumed more individuals of *** than the eggs of *** results on the effect of three constant temperatures and two preys on the biology of *** will share to improve the rearing of *** in biological control agents in China and share to suppress the population of pests in field and greenhouse.

关键词： Orius similis Zheng biological characteristics feeding consumption

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Assessment of pesticide Use against Tephritidae Fruit Fly and Other pest among Small-scale Solanaceous Vegetable Farmers in Bugorhe-Kabare the Democratic Republic of Congo

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NASS Journal of Agricultural Sciences 2022年第2期4卷 27-35页

作者： Rubabura,K.JA. Ndatabaye,L.F. Lina,A.A. Muhigwa,B.JB. Agricultural Entomology laboratory Entomology SectionResearch Centre in Natural Sciences(CRSN-Lwiro)Democratic Republic of Congo Department of Biology State University of BukavuDemocratic Republic of Congo

Ninety-six farmers interviewed in Kabare,east of the DR Congo during *** majority were males(79.17%),ranging 30 to 60 years,used different pesticides in vegetable farms and the main solanaceous crops cultivated is *** use of insecticide and fungicide were high,with many different formulations of the different class types recorded in use,(20%)endocrine disruptors,(40%)cholinesterase inhibitors,(35%)carcinogen and potential carcinogens suspected to be.A lot of out of those pesticides are unregistered for general *** applied pesticide once a week and they didn’t have specific *** skin effects,headaches and dizziness are *** do not have a good system of pesticide packaging *** reducing pesticide application,we propose options of agro *** suggest that the Congolese government must create a quarantine,control and surveillance service for phytosanitary products,fruits and vegetables within the DRC country and at these ***,it needs urgent action from the federal and regional governments to formulate policy,design legislation,and enforcing for its implementation concerning the supply,transportation,storage,appropriateness,and application of harmful pesticides.

关键词： pesticide Market gardeners’crops Farmer’s small holders Endocrine disruptors Cholinesterase inhibitors

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Study of a crop-pest-natural enemy model with Z-type control——An approach to pest management

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International Journal of Biomathematics 2023年第4期16卷 175-207页

作者： Dibyendu Sekhar Mandal Sudip Samanta Rana D.Parshad Abdennasser Chekroun Mohamed Helal Joydev Chattopadhyay Department of Mathematics AmityUniversity Maharashtra Mumbai Maharashtra 410206India Agricultural and Ecological Research Unit Indian Statistical Institute 203B.T.Road Kolkata 700108India Department of Mathematics Bankura University WestBengal722155India Department of Mathematics Iowa State University AmesIA 50011USA Laboratoire d'Analyse Nonlineaire et Mathematiques Appliquees University of Tlemcen Tlemcen 13000Algeria Biomathematics laboratory Universite Sidi Bel-Abbes P.B.8922000Algeria

In this study,the Z-type control method is applied to an intraguild crop-pest-natural enemy model,assuming that the natural enemy can predate on both crop and pest *** this purpose,the indirect Z-type controller is considered in the natural enemy *** providing the design function for the crop-pest-natural enemy model with Z-control,we find the analytical expression of the update *** findings indicate that the uncontrolled system can produce chaos through perioddoubling bifurcation due to crop over-consumption by the pest *** draw a Poincare map to confirm the occurrence of chaos and compute the maximum Lyapunov *** the observations further indicate that the pest population can be controlled by using an indirect Z-control mechanism in the natural enemy population,we postulate that,if natural enemy abundance can be governed by the update parameter,any desired pest population abundance can be achieved through the proposed Z-type controller,thus controlling the *** verify these assertions,extensive numerical simulations are performed to explore the potential for practical application of the proposed Z-type controller.

关键词： Crop-pest-natural enemy model Z-type control numerical simulations pest control

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Floral biology, breeding system and pollination ecology of an endangered tree Tetracentron sinense Oliv. (Trochodendraceae)

Floral biology, breeding system and pollination ecology of a...

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生态文明建设中的植物学：现在与未来——中国植物学会第十...

作者： Xiaohong Gan Lingling Cao Xia Zhang Huaichun Li College of Life Science China West Normal University Key laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education) China West Normal University Library of China West Normal University

In order to explore the endangered factors related to pollination, and to provide important information for its conservation, the floral biology, breeding system and pollination ecology of T. sinense in Dafengding Natural Reserve were investigated. T. sinense usually flowers by the beginning of June, and the flowering period of the population is about two months, and the florescence of florets lasted for 15 to 24 days with delicate fragrance, and fruiting period for about 3 months. The flowering dynamic of a single flower could be divided into the folowing stages: flower buds with closed sepals, sepals opening, style elongating and reflexing, the elongation of bilateral stamens, the dehiscence of bilateral anthers, the elongation of upper and lower stamens, dehiscence of bilateral anthers, the termination. During anthesis, the recurved styles were gradually positioned lower than the anthers, and the stigmas have receptivity during the whole period of anthesis. The pollen/ovule ratio is 720±28, and the outcrossing index is three. controlled pollination experiment showed that T. sinense is self-compatible, and outbreeding fertile, with no indication of agamospermy. The germination rate of seed from xenogamy and control treatment is better than that from autogamy treatment. The occurrence of natural pollination both by wind and insects indicates T. sinense isambophilous and the maintenance of ambophily in T. sinense may attribute to the decrease of population density. insect pollinators were predominantly represented by Coleoptera, Diptera and Hymenoptera. Syrphid fly and bees were the main effective pollinators. The results suggested that T. sinense exhibits a mixed-mating system with outcrossing and self-pollination. In the natural population, the space between scattered individuals of T. sinense is usually bigger, and its flowering period is just coincident with the rainy season, which may have negative effect on the outcrossing (wind- and insect-pollination). Thus,

关键词： Floral biology Breeding system Pollination Tetracentron sinense Oliv. Natural regeneration.

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Global attractivity of the predator-prey system for cotton aphid and seven-spot ladybird beetle with stage structure

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International Journal of Biomathematics 2018年第1期11卷 279-300页

作者： Lifei Zheng Guixin Hu Huiyan Zhao M. K. D.K. Piyaratne Aying Wan College of Science Northwest A&F University Yangling Shaan'xi 712100 P. R. China School of Mathematics and Information Science Henan Polytechnic University (HPU) Jiaozuo 454000 P. R. China State Key laboratory of Crop Stress in Arid Areas Northwest A& F University Yangling Shaan'xi 712100 P. R. China Faculty of Agriculture University of Ruhuna Mapalana Kamburupitiya Sri Lanka Department of Mathematics Hulunbeir College Hailar Inner Mongolia 021008 P. R. China

It is well known that the cotton aphid is the major pest in cotton fields of Northwest China, and seven-spot ladybird is an important natural enemy among the various possible natural enemies of cotton aphid. In order to increase the applications of population dynamics in integrated pest management and control the cotton aphids biologically, we need to understand the population dynamics of cotton aphid and their natural enemies. A delay predator–prey system on cotton aphid and seven-spot ladybird beetle are proposed in this paper. Based on the comparison theorem and an iterative method, we investigate the global attractivity of the equilibrium points which have important biological meanings. Furthermore, some numerical simulations were carried out to illustrate and expand our theoretical results, in which a conjecture to generalize the well-known Theorem 16.4 in H. R. Thiemes book was put forward, which was taken as the open problem. The numerical simulations show coexistence of periodic solution, confirming the theoretical prediction.

关键词：棉花蚜虫食肉动物甲壳虫瓢虫系统猎物人口动力学结构

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Glycoside hydrolase family 18 and 20 enzymes are novel targets of the traditional medicine berberine

Glycoside hydrolase family 18 and 20 enzymes are novel targe...

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中国生物化学与分子生物学会第十二届全国会员代表大会暨2...

作者： Yanwei Duan Tian Liu Yong Zhou Tongyi Dou Qing Yang State Key laboratory of Fine Chemical Engineering School of Life Science and Biotechnology and School of SoftwareDalian University of Technology School of Life Science and Medicine Dalian University of Technology Institute of Plant Protection Chinese Academy of Agricultural Sciences

Berberine is a traditional medicine that has multiple medicinal and agricultural ***,little is known about whether berberine can be a bioactive molecule toward carbohydrate-active enzymes,which play numerous vital roles in the life *** this study,berberine and its analogs were discovered to be competitive inhibitors of glycoside hydrolase family 20 β-N-acetyl-D-hexosaminidase(GH20 Hex) and GH18 chitinase from both humans and the insect pest Ostrinia *** and its analog SYSU-1 inhibit insect GH20 Hex from ***(Of Hex1),with Ki values of 12 and 8.5 μM,***-crystallization of berberine and its analog SYSU-1 in complex with Of Hex1 revealed that the positively charged conjugate plane of berberine forms π-π stacking interactions with Trp,which are vital to its inhibitory ***,the 1,3-dioxole group of berberine binds an unexplored pocket formed by Trp,Trp and Val,which also contributes to its inhibitory *** was also found to be an inhibitor of human GH20 Hex(Hs HexB),human GH18 chitinase(Hs Cht and AMCase) and insect GH18 chitinase(Of ChtI).Besides GH18 and GH20 enzymes,berberine was shown to weakly inhibit human GH84 O-GlcNAcase(Hs OGA) and Saccharomyces cerevisiae GH63 α-Glucosidase I(Sc Glu I).By analyzing the published crystal structures,berberine was revealed to bind with its targets in an identical mechanism,namely via π-π stacking and electrostatic interactions with the aromatic and acidic residues in the binding *** paper reports new molecular targets of berberine and may provide a berberine-based scaffold for developing multi-target drugs.

关键词： berberine glycoside hydrolase β-N-acetyl-D-hexosaminidase chitinase inhibitor

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Plastid-mediated crop protection by RNA interference

Plastid-mediated crop protection by RNA interference

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2016年全国植物生物学大会

作者： Jizhen Wei Yuyong Wu Sher Afzal Khan David G.Heckel Ralph Bock Jiang Zhang Hubei Collaborative Innovation Center for Green Transformation of Bio-resources Hubei Key Laboratory of Industrial BiotechnologyCollege of Life SciencesHubei University Max Planck Institute for Chemical Ecology Max Planck Institute of Molecular Plant Physiology

Plastid(chloroplast) genetic engineering has a great promise in plant biotechnology. It has several attractive advantages such as high-level of transgene expression owing to the polyploidy of the plastid genetic system,transgene containment via maternal inheritance and absence of gene silencing and pleiotropic effects. It has been demonstrated that double-stranded RNAs(dsRNAs) targeted against essential genes can trigger a lethal RNAi response in insect pests. Previously,we found chloroplasts can be capable of stably accumulating long dsRNAs,in which case dsRNA expression from the plastid genome provide better protection against Colorado potato beetle(CPB),a notorious agricultural pest,than dsRNA expression from the nuclear genome(Zhang et al.,Science,2015). Encouraged by this primary success,we aimed to employ this strategy to target another important insect pest,cotton bollworm(CBW). However,feeding transplastomic plants expressing dsRNA against essential genes of CBW did not cause the gene silencing and suppress the growth of CBW larvae. While no effective RNAi responses were observed in CBW,we found that a secreted RNA nuclease from midgut cell of CBW larvae can quickly degrade the ingested dsRNA,which might impede the effect of RNAi. From this findings,we proposed an approach how to escape the activity of this RNA nuclease and control the insects that showed no or less sensitivity to RNAi. In the end,I will summarize an updated working model of plastid-mediated crop protection and the potential application of this technology.

关键词： crop protection plastid genetic engineering RNAi RNA nuclease

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