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Mass Spectrometry Analysis of Newly Emerging Coronavirus HCoV-19 Spike Protein and Human ACE2 Reveals Camouflaging Glycans and Unique Post-Translational Modifications

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Engineering 2021年第10期7卷 1441-1451页

作者： Zeyu Sun Keyi Ren Xing Zhang Jinghua Chen Zhengyi Jiang Jing Jiang Feiyang Ji Xiaoxi Ouyang Lanjuan Li State Key laboratory for Diagnosis and Treatment of Infectious Diseases National Clinical Research Center for Infectious DiseasesCollaborative Innovation Center for Diagnosis and Treatment of Infectious DiseaseThe First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310011China Department of biophysics&Center of Cryo-Electron Microscopy School of MedicineZhejiang UniversityHangzhou 310011China

The coronavirus disease 2019(COVID-19)pandemic has led to worldwide efforts to understand the biological traits of the newly identified human coronavirus(HCoV-19)*** this mass spectrometry(MS)-based study,we reveal that out of 21 possible glycosites in the HCoV-19 spike protein(S protein),20 are completely occupied by N-glycans,predominantly of the oligomannose *** seven glycosylation sites in human angiotensin I converting enzyme 2(hACE2)were found to be completely occupied,mainly by complex ***,glycosylation did not directly contribute to the binding affinity between HCoV-19 S protein and *** post-translational modification(PTM)was identified,including multiple methylated sites in both proteins and multiple sites with hydroxylproline in *** structural models of HCoV-19 S protein and hACE2 were built by adding N-glycan and PTMs to recently published cryogenic electron microscopy *** PTM and glycan maps of HCoV-19 S protein and hACE2 provide additional structural details for studying the mechanisms underlying host attachment and the immune response of HCoV-19,as well as knowledge for developing desperately needed remedies and vaccines.

关键词： N-glycosylation COVID-19 Spike protein hACE2 Structure

Mechanical stretching boosts expansion and regeneration of intestinal organoids through fueling stem cell self-renewal

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Cell Regeneration 2022年第1期11卷 399-412页

作者： Fanlu Meng Congcong Shen Li Yang Chao Ni Jianyong Huang Kaijun Lin Zanxia Cao Shicai Xu Wanling Cui Xiaoxin Wang Bailing Zhou Chunyang Xiong Jihua Wang Bing Zhao Shandong Key laboratory of biophysics Institute of BiophysicsDezhou UniversityDezhou 253023China Department of Mechanics and Engineering Science College of EngineeringPeking UniversityBeijing 100871China State Key laboratory of Genetic Engineering School of Life SciencesZhongshan HospitalFudan UniversityShanghai 200438China Beijing innovation Center for Engineering Science and Advanced Technology College of EngineeringPeking UniversityBeijing 100871China Wenzhou Institute University of Chinese Academy of SciencesOujiang LaboratoryWenzhou 325000ZhejiangChina

Intestinal organoids,derived from intestinal stem cell self-organization,recapitulate the tissue structures and behav-iors of the intestinal epithelium,which hold great potential for the study of developmental biology,disease modeling,and regenerative *** intestinal epithelium is exposed to dynamic mechanical forces which exert profound effects on gut ***,the conventional intestinal organoid culture system neglects the key role of mechanical microenvironments but relies solely on biological ***,we show that adding cyclic stretch to intestinal organoid cultures remarkably up-regulates the signature gene expression and proliferation of intestinal stem ***,mechanical stretching stimulates the expansion of SOX9+progenitors by activating the Wnt/β-Catenin *** data demonstrate that the incorporation of mechanical stretch boosts the stemness of intestinal stem cells,thus benefiting organoid *** findings have provided a way to optimize an organoid generation system through understanding cross-talk between biological and mechanical factors,paving the way for the application of mechanical forces in organoid-based models.

关键词： Mechanical stretching Intestinal organoid Lgr5+stem cell Regeneration Wnt/β-catenin signaling

hTAC internalizes via both clathrin-dependent and clathrin-independent endocytosis in mammalian cells

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Protein & Cell 2018年第10期9卷 896-901页

作者： Xinyu Zhu Min Li Xiaojun Xu Rui Zhang Xiaofei Zhang Zhuo Ma Jingze Lu Tao Xu Junjie Hou Eli Song Key laboratory of Molecular biophysics of the Ministry of Educa-tion College of Life Science and Technology Huazhong Universityof Science and Technology Wuhan 430074 China National laboratory of Biomacromolecules CAS Center forExcellence in Biomacromolecules Institute of Biophysics ChineseAcademy of Sciences Beijing 100101 China College of Life Sciences University of Chinese Academy ofSciences Beijing 100049 China

Dear Editor, Endocytosis is a crucial process employed by cells to internal- ize nutrients and turnover membrane components and is essential for many functions, including nutrient uptake, signal transduction, cytokinesis, morphogenesis, cell adhesion and migration. Endocytosis is classified as clathrin-dependent endocytosis （CDE） or clathrin-independent endocytosis （CIE） according to its dependence on clathrin. Several different CIE pathways have been proposed, including caveolin-dependent endocytosis, flotillin-dependent endocytosis, the clathrin-inde- pendent carrier pathway, ARF6-dependent endocytosis, phagocytosis, macropinocytosis, the IL2RI3 pathway （Doherty and McMahon, 2009）, the newly identified fast endophilin-me- diated endocytosis pathway （Boucrot et al., 2015） and the EGFR-NCE pathway （Caldieri et al., 2017）.

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Mps1 dimerization and multisite interactions with Ndc80 complex enable responsive spindle assembly checkpoint signaling

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Journal of Molecular Cell Biology 2020年第7期12卷 486-498页

作者： Ping Gui Divine M.Sedzro Xiao Yuan Sikai Liu Mohan Hei Wei Tian Najdat Zohbi Fangwei Wang Yihan Yao Felix O.Aikhionbare Xinjiao Gao Dongmei Wang Xuebiao Yao Zhen Dou MOE Key laboratory of Membraneless Organelle and Cellular Dynamics Hefei National Laboratory for Physical Sciences at the MicroscaleSchool of Life SciencesUniversity of Science and Technology of ChinaHefei 230027China Keck Center for Cellular Dynamics and Organoids Plasticity Morehouse School of MedicineAtlantaGA 30310USA Key laboratory of Molecular biophysics of the Ministry of Education College of Life Science and TechnologyHuazhong University of Science and TechnologyWuhan 430074China Life Sciences Institute and innovation Center for Cell Signaling Network Zhejiang UniversityHangzhou 310058China

Error-free mitosis depends on accurate chromosome attachment to spindle microtubules,which is monitored by the spindle assembly checkpoint(SAC)*** an upstream factor of SAC,the precise and dynamic kinetochore localization of Mps1 kinase is critical for initiating and silencing SAC ***,the underlying molecular mechanism remains ***,we demonstrated that the multisite interactions between Mps1 and Ndc80 complex(Ndc80C)govern Mps1 kinetochore ***,we identified direct interaction between Mps1 tetratricopeptide repeat domain and *** further identified that Mps1 C-terminal fragment,which contains the protein kinase domain and C-tail,enhances Mps1 kinetochore ***,Mps1 C-terminal fragment mediates its *** of C-tail attenuates the kinetochore targeting and activity of Mps1,leading to aberrant mitosis due to compromised SAC *** together,our study highlights the importance of Mps1 dimerization and multisite interactions with Ndc80C in enabling responsive SAC signaling.

关键词： mitosis spindle assembly checkpoint kinetochore Mps1 kinase Ndc80 complex

Bipolar electrochemistry in confined nanospace

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Science China Chemistry 2018年第4期61卷 379-380页

作者： Dongping Zhan State Key laboratory of Physical Chemistry of Solid Surfaces Collaborative Innovation Centre of Chemistry for Energy MaterialsDepartment of ChemistryCollege of Chemistry and Chemical EngineeringXiamen University

Nanopores employ a confined space for electrochemical sensing of high-throughput individual biomolecules in *** most general mechanism of nanopore sensing is based on a volume exclusion effect.

关键词：电气化学双极

The structure differences of Japanese encephalitis virus SA14 and SA14-14-2 E proteins elucidate the virulence attenuation mechanism

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Protein & Cell 2019年第2期10卷 149-153页

作者： Xinyu Liu Xin Zhao Rui Na Lili Li Eberhard Warkentin Jennifer Witt Xu Lu Yongxin Yu Yuquan Wei Guohong Peng Yuhua Li Junzhi Wang National Institutes for Food and Drug Control Beijing 102609China Department of Molecular Membrane Biology Max Planck Institute of Biophysics60438 Frankfurt am MainGermany State Key laboratory of Biotherapy and Cancer Center West China HospitalSichuan Universityand Collaborative Innovation Center for BiotherapyChengdu 610041China

Dear Editor,Japanese encephalitis(JE)is a mosquito-borne acute neurological infectious disease caused by the Japanese encephalitis virus(JEV).Globally,68,000 cases of the disease are estimated each year,with a fatality rate of as high as 30%and with approximately 30%-50%of survivors suffering from severe neurological sequelae(WHO,2015).

关键词： Editor JE JEV

Discovering Cathodic Biocompatibility for Aqueous Zn–MnO_(2) Battery:An Integrating Biomass Carbon Strategy

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Nano-Micro Letters 2024年第6期16卷 111-126页

作者： Wei Lv Zilei Shen Xudong Li Jingwen Meng Weijie Yang Fang Ding Xing Ju Feng Ye Yiming Li Xuefeng Lyu Miaomiao Wang Yonglan Tian Chao Xu Institute of Energy Power innovation North China Electric Power UniversityBeijing 102206People’s Republic of China Department of Power Engineering School of EnergyPower and Mechanical EngineeringNorth China Electric Power UniversityBaoding 071003People’s Republic of China Key laboratory of RNA Biology Institute of BiophysicsChinese Academy of SciencesBeijing 100101People’s Republic of China Collaborative innovation Center of Integrated Exploitation of Bayan Obo Multi‑Metal Resources Inner Mongolia University of Science and TechnologyBaotou 014010People’s Republic of China

Developing high-performance aqueous Zn-ion batteries from sustainable biomass becomes increasingly vital for large-scale energy storage in the foreseeable ***,γ-MnO_(2) uniformly loaded on N-doped carbon derived from grapefruit peel is successfully fabricated in this work,and particularly the composite cathode with carbon carrier quality percentage of 20 wt%delivers the specific capacity of 391.2 mAh g^(−1)at 0.1 A g^(−1),outstanding cyclic stability of 92.17%after 3000 cycles at 5 A g^(−1),and remarkable energy density of 553.12 Wh kg^(−1) together with superior coulombic efficiency of~100%.Additionally,the cathodic biosafety is further explored specifically through in vitro cell toxicity experiments,which verifies its tremendous potential in the application of clinical ***,Zinc ion energy storage mechanism of the cathode is mainly discussed from the aspects of Jahn–Teller effect and Mn domains distribution combined with theoretical analysis and experimental ***,a novel perspective of the conversion from biomass waste to biocompatible Mn-based cathode is successfully developed.

关键词： Aqueous Zn-ion batteries Biocompatibility Jahn-Teller effect Mn domains γ-MnO_(2)

Uric formaldehyde levels are negatively correlated with cognitive abilities in healthy older adults

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Neuroscience Bulletin 2014年第2期30卷 172-184页

作者： Jing Yu Tao Su Ting Zhou Yingge He Jing Lu Juan Li Rongqiao He Center on Ageing Psychology Key Laboratory of Mental Health Institute of Psychology Chinese Academy of Sciences Faculty of Psychology Southwest University State Key laboratory of Brain and Cognitive Science Institute of Biophysics Chinese Academy of Sciences The University of Chinese Academy of Sciences

Recent studies have shown that the abnormal accumulation of endogenous formaldehyde could be a critical factor in age-related cognitive decline. The aim of this study was to estimate the correlation between uric formaldehyde and general cognitive abilities in a community-based elderly population, and to measure the extent and direction in which the correlation varied with demographic characteristics. Using a double-blind design, formaldehyde in human urine was analyzed by high-performance liquid chromatography （n = 604）, and general cognitive abilities were measured using the Montreal Cognitive Assessment （MoCA）. Demographic characteristics, in terms of age, gender, residential region, and education were taken into consideration. We found that uric formaldehyde levels were inversely correlated with the MoCA score, and the concentration varied with demographic features： higher odds of a high formaldehyde level occurred among the less educated and those living in old urban or rural areas. In cytological experiments, the level of cellular formaldehyde released into the medium increased as SH-SY5Y and BV2 cells were incubated for three days. Formaldehyde in excess impairedthe processes of N2a cells and neurites of primary cultured rat hippocampal cells. However, removal of formaldehyde markedly rescued and regenerated the processes of N2a cells. These results demonstrated a negative correlation between the endogenous formaldehyde and general cognitive abilities. High formaldehyde levels could be a risk factor for cognitive impairment in older adults, and could be developed as a non-invasive marker for detection and monitoring of age-related cognitive impairment.

关键词： uric formaldehyde the MontrealCognitive Assessment education learning livingregion

AtPQT11,a P450 enzyme,detoxifies paraquat via N-demethylation

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Journal of Genetics and Genomics 2022年第12期49卷 1169-1173页

作者： Yi-Jie Huang Yue-Ping Huang Jin-Qiu Xia Zhou-Ping Fu Yi-Fan Chen Yi-Peng Huang Aimin Ma Wen-Tao Hou Yu-Xing Chen Xiaoquan Qi Li-Ping Gao Cheng-Bin Xiang Division of Life Sciences and Medicine Division of Molecular&Cell BiophysicsHefei National Science Center for Physical Sciencesat the MicroscaleMOE Key Laboratory for Membraneless Organelles and Cellular DynamicsUniversity of Science and Technologyof ChinaThe Innovation Academy of Seed DesignChinese Academy of SciencesHefeiAnhui 230027China School of Life Science Anhui Agricultural UniversityHefeiAnhui 230036China Key laboratory of Plant Molecular Physiology Institute of BotanyThe Innovation Academy of Seed DesignChinese Academy of SciencesBeijing 100093China

Paraquat(1,1’-dimethyl-4,4’-bipyridinium dichloride,PQ)has been widely used as a broad spectrum nonselective herbicide in agriculture for decades(Baldwin et al.,1968).Plants absorb PQ from their environment and transport to the chloroplasts,the site of its action where it competes for electrons from photosystem I(PSI)and cyclically generates superoxide that is converted to H2O2by superoxide dismutases(SODs),resultantly accumulating a large amount of reactive oxygen species(ROS)and thereby killing plants(Farrington et al.,1973).

关键词： thereby converted resultant