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Fluorescence-based PCR Detection of Goat Milk Powder Mixed with co. Milk

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Asian Agricultural Research 2021年第8期13卷 43-45,48页

作者： Yunxia WANG Lijuan JING Zhijun LI Cuizhi LI Zhiyong LU Lijun LIU Quality Management Department Inner Mongolia Yili Industrial Group Co. Ltd. Hohhot 010110 China Quality Management Department of Yogurt Division Inner Mongolia Yili Industrial Group Co. Ltd. Hohhot 010110 China

[Objectives]To amplify and identify the specific gene fragments of cattle and sheep by real-time fluorescence-based PCR,and quickly and accurately identify the adulteration problem of goat milk powder acco.ding to the differences of mitochondrial genes among different species.[Methods]The specificity,limit of detection and repeatability test were carried out by isolating somatic cells,extracting template DNA and determining the reaction co.ditions of real-time fluorescence-based PCR.[Results]The real-time fluorescence-based PCR method co.ld be used to identify the bovine and ovine-derived materials of milk powder with high specificity,high sensitivity and good repeatability,and the limit of detection of milk co.ponents in goat milk powder co.ld reach 0.01%.[co.clusions]The real-time fluorescence-based PCR method can be used to quickly and accurately detect the milk co.ponents in goat milk powder,and provide some technical support for the adulteration detection of dairy industry.

关键词： Goat milk powder co. milk Real-time fluorescence-based PCR Animal origin

Simultaneous Determination of co.tent of VK_(1) and VK_(2) in Milk and Dairy Products by High Performance Liquid Chromatography

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Asian Agricultural Research 2022年第7期14卷 63-68,71页

作者： Huili GONG Guoxia DUAN Lijun LIU Shuo TANG Cuizhi LI Zhiyong LU inner mongolia yili industrial group co.Ltd. Hohhot 010110China

[Objectives]To make simultaneous determination of vitamin K_(1)(VK_(1))and vitamin K_(2)(VK_(2),mainly MK-4,MK-7 and MK-9)in milk and dairy products.[Methods]A high performance liquid chromatographic method was developed for the simultaneous determination of vitamin K_(1)(VK_(1))and vitamin K_(2)(VK_(2),mainly MK-4,MK-7 and MK-9)in milk and dairy *** enzymatic digestion,the samples were extracted with hexane for VK_(1),MK-4,MK-7 and MK-9,subjected to gradient elution at excitation wavelength 243 nm and emission wavelength 430 nm,detected by high performance liquid chromatography with fluorescence detector and quantified by external standard method.[Results]The linearity of VK_(1),MK-4,MK-7 and MK-9 was good in the co.centration range of 2.5-1000 ng/mL with the co.relation co.fficients greater than 0.999;The relative standard deviations(RSD)of VK_(1),MK-4,MK-7 and MK-9 in milk powder,liquid milk and yogurt were 1.32%-5.05%,1.10%-2.48% and 2.20%-3.47%,respectively;the reco.ery rates of VK_(1),MK-4,MK-7 and MK-9 at different levels in milk powder,liquid milk and yogurt were 81.1%-108%,81.8%-103%and 82.1%-99.2%,respectively.[co.clusions]The method is rapid,accurate,reproducible and capable of simultaneous determination of VK_(1),MK-4,MK-7 and MK-9.

关键词： High performance liquid chromatography(HPLC) Vitamin K_(1) Vitamin K_(2) MK-4 MK-7 MK-9

Characterization of the co.e microflora and nutrient co.position in packaged pasteurized milk products during storage

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Food Science and Human Wellness 2023年第4期12卷 1279-1286页

作者： Ruixue Ding Shanshan Yang Lijuan Geng Yumeng Liu Baoping He Liyun Liu Xiqing Yue Rina Wu Junrui Wu Liaoning Engineering Research Center of Food Fermentation Technology Shenyang Key Laboratory of Microbial Fermentation Technology InnovationCollege of Food ScienceShenyang Agricultural UniversityShenyang 110866China Shenyang Institute for Food and Drug co.trol Shenyang 110122China inner mongolia yili industrial group co. Ltd.Hohhot 010080China China Mengniu Dairy co. Ltd.Hohhot 010000China

Pasteurized milk co.tains co.plex microbial co.munities affected by sterilization and storage *** co.plex microflora may be the possible reason that pasteurized dairy products are highly prone to *** this study,packaged pasteurized milk products co.lected from dairy processing factories in China were stored at 0,4,10,15,and 25℃ for 0−15 days and subjected to microbial identification using high-throughput ***,6 phyla and 44 genera were identified as the dominant ***,the changes in nutritional co.position of the pasteurized milk,including in 16 free amino acids,7 taste values,and 8 chemical co.stituents,were analyzed using principal co.ponent and multi-factor *** Pearson co.relation analysis identified Pseudomonas,Aeromonas,Paenibacillus,and Serratia genera as the co.e functional microbiota that significantly affects the nutritional co.position of pasteurized ***,the results provide a co.prehensive understanding of the safety and shelf-life of stored pasteurized milk.

关键词： Pasteurized milk Storage co.ditions Microbiota High-throughput sequencing(HTS)

Analysis of internal crack in a six-ton P91 ingot

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China Foundry 2016年第3期13卷 191-198页

作者： Jing-an Yang Hou-fa Shen Bai-cheng Liu Ya-dong Xu Yong-ping Hu Bing-wang Lei School of Materials Science and Engineering Tsinghua University Special Material Institute Inner Mongolia North Heavy Industries Group Co. Ltd.

P91 is a new kind of heat-resistant and high-tensile steel. It can be extruded after ingot casting and can be widely used for different pipes in power plants. However, due to its mushy freezing characteristics, a lack of feeding in the ingot center often generates many defects, such as porosity and crack. A six-ton P91 ingot was cast and sliced, and a representative part of the longitudinal section was inspected in more detail. The morphology of crack-like defects was examined by X-ray high energy industrial CT and reco.structed by 3D software. There are f ive main portions of defects larger than 200 mm^3, four of which are interco.nected. These initiated from co.tinuous liquid f ilm, and then were torn apart by excessive tensile stress within the brittle temperature range(BTR). The 3D FEM analysis of thermo-mechanical simulation was carried out to analyze the formation of porosity and internal crack defects. The results of shrinkage porosity and Niyama values revealed that the center of the ingot suffers from inadequate feeding. Several criteria based on thermal and mechanical models were used to evaluate the susceptibility of hot crack formation. The Clyne and Davies' criterion and Katgerman's criterion successfully predicted the high hot crack susceptibility in the ingot center. Six typical locations in the longitudinal section had been chosen for analysis of the stresses and strains evolution during the BTR. Locations in the defects region showed the highest tensile stresses and relative high strain values, while other locations showed either low tensile stresses or low strain values. In co.clusion, hot crack develops only when stress and strain exceed a threshold value at the same time during the BTR.

关键词： hot crack industrial CT liquid film thermo-mechanical simulation steel ingot

Bifidobacterium lactis BL-99 protects mice with osteoporosis caused by co.itis via gut inflammation and gut microbiota regulation

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Bifidobacterium lactis BL-99 protects mice with osteoporosis...

2′-Fuco.yllactose modulates the function of intestinal microbiota to reduce intestinal permeability in mice co.onized by feces from healthy infants

中国营养学会第十五届全国营养科学大会

作者： Hui Lan Wei-Hsien Liu Hanying Zheng Haotian Feng Wen Zhao Wei-Lian Hung Hongwei Li School of Public Health Xiamen University inner mongolia yili industrial group co. Ltd.

Osteoporosis is a metabolic systemic skeletal disease with high incidence and co.plex etiology in China and the world. It is closely related to age, environment, genetics, diseases and other influencing factors. Among them, the incidence of osteoporosis in patients with gastrointestinal diseases is high. Due to intestinal inflammation, the intestinal microenvironment changes, and the gut microbiota is imbalanced, which affects the absorption and deposition of calcium and the process of bone metabolism. This experiment hypothesized that probiotic intervention co.ld alter the co.position and abundance of gut microbiota, alleviate gut inflammation, and thereby prevent the development of osteoporosis. In the experiment, the ability of Bifidobacterium lactis BL-99 to prevent bone loss was analyzed, and the possible protective mechanism was explored. Methods 6-week-old male C57 BL/6 J mice were divided into 5 groups acco.ding to their initial body weight, with 8 mice in each group. The experiment used dextran sodium sulfate(DSS) chemical induction, 2% and 1% DSS solutions were free to drink water for one week, respectively, to establish an ulcerative co.itis(UC) mouse animal model, and a week before modeling and during modeling Different types and co.centrations of probiotics were administered by gavage for three weeks. During the modeling period, the body weight of the mice was measured every day, and the loose and bloody stools of the mice were observed. Blood, co.on, femur and tibia were co.lected at the end of three weeks. ELISA was used to detect the expression levels of inflammatory factors in serum and co.on tissue;HE staining was used to observe the pathological changes of co.on and tibia;Micro-CT was used to analyze the changes of bone microstructure;16 SrDNA gene sequencing was used to analyze the changes of gut microbiota;Western The expression level of intestinal barrier proteins was detected by Blot method. Results were expressed as mean ± standard deviation(

关键词： Probiotics Osteoporosis Ulcerative co.itis Gut microbiota

来源： cnki会议评论

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Food Science and Human Wellness 2023年

作者： Qingxue Chen Liu Yang Fangqin Xiang Ignatius Man-Yau Szeto Yalu Yan Biao Liu Jinju Cheng Lu Liu Bailiang Li Sufang Duan Food co.lege， Northeast Agricultural University National Center of Technology Innovation for Dairy Hohhot inner mongolia yili industrial group， co.Ltd.， yili Maternal and Infant Nutrition Institute (YMINI) Key Laboratory of Dairy Science， Ministry of Education， Northeast Agricultural University

2′-Fuco.yllactose (2’-FL) shows the potential to support intestinal health as a natural prebiotic that bridges the gap between infant formula feeding and breastfeeding. However， the effect and mechanism of 2’-FL in improving intestinal permeability are not clear. In this study， we co.structed human microbiota-associated (HMA) mouse models by co.onizing healthy infant feces in mice with antibiotic-depleted intestinal microbiota. The protective effect of 2’-FL on the intestinal permeability was explored using the HMA mouse models， and the co.bination of metagenomics was used to analyze the possible mechanisms by which the microorganisms reduced the intestinal permeability. The results showed that 2’-FL decreased the co.centration of markers of intestinal permeability (enterotoxin and DAO) and increased the expression levels of tight junctions (occludin and claudin). Metagenomics revealed the enrichment of Bifidobacterium and increased the expression of glyco.ide hydrolases (GHs)， including GH31， GH28， and GH5. In co.clusion， 2’-FL strengthened intestinal permeability function by improving microbiota co.position to co.trol the translocation of harmful substance.

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A Model of Spray Tool and a Parameter Optimization Method for Spraying Path Planning

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International Journal of Automation and co.puting 2021年第6期18卷 1017-1031页

作者： Ru-Xiang Hua Wei Zou Guo-Dong Chen Hong-Xuan Ma Wei Zhang School of Artificial Intelligence University of Chinese Academy of SciencesBeijing 100080China Institute of Automation Chinese Academy of SciencesBeijing 100190China Shandong Jonkin Intelligent Technology co. LtdWeihai 264200China inner mongolia First Machinery group of co. LtdBaotou 014000China

The digital camouflage spraying of special vehicles carried out by robots can greatly improve the spraying efficiency, spraying quality, and rapid adaptability to personalized patterns. The selection of spray tool and the accuracy of the adopted mathematical spray tool model has a great impact on the effectiveness of spray path planning and spraying quality. Since traditional co.ical spray tool models are not suitable for spraying rectangular digital camouflage, acco.ding to the characteristics of digital camouflage, the co.ting thickness cumulative distribution model of strip nozzle spray tool for 2 D plane spraying and 3 D surface spraying is derived, and its validity is verified by simulation. Based on the accumulation velocity model of the co.ting thickness(AVCT) on the curved surface and aiming at spraying path planning within the same surface and different surfaces, a path parameter optimization method based on co.ting uniformity evaluation of adjacent path overlapping area is proposed. co.bined with the vehicle surface model, parameters such as path interval, spray tool angle and spray tool motion velocity can be calculated in real-time to ensure uniform co.ting. Based on the known local three-dimensional model of vehicle surface and the co.prehensive spraying simulation, the validity of the purposed models: the co.ting thickness on the adjacent path area(CTAPA), the co.ting thickness on the intersection of two surfaces(CTITS), the co.ting thickness on the intersection of a plane and a surface(CTIPS), and the optimization method of path parameters are verified. The results show that co.pared with the traditional spray tool, the strip nozzle can better ensure the uniformity of the co.ting thickness of digital camouflage spray. Finally, acco.ding to a practical spraying experiment, the results prove that the proposed models not only are effective but also meet the practical industrial requirements and are of great practical value.

关键词： Digital camouflage spraying robot path optimization parameter setting co.ting thickness uniformity

Building Rare Earth industrial Chain

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China Rare Earth Information 2009年第12期15卷 1-2页

作者： ZHANG Zhong General manager of inner mongolia Baogang Rare Earth（group） Hi-Tech co. Ltd

Since the seco.d half of 2008,the financial storm induced by the subprime mortgage crisis in the US rapidly spread to the *** large sectors pushing RE co.sumption,like metallurgy industry,automotive industry,petroleum... 详细信息

关键词：产业链稀土能源消费冶金工业汽车工业石油化工电子工业金融

Physico.hemical properties and volatile co.ponents of pea flour fermented by Lactobacillus rhamnosus L08

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Food Bioscience 2022年第2期46卷 793-800页

作者： Mengqi Pei Zifu Zhao Song Chen E.I.Reshetnik S.L.Gribanova Chun Li Guofang Zhang Libo Liu Lin Zhao Key Laboratory of Dairy Sciences Ministry of EducationCollege of Food SciencesNortheast Agricultural UniversityHarbin150030China inner mongolia yili group co.Ltd Hohhot010080China Far Eastern State Agrarian University Blagoveshchensk675005Russia Heilongjiang Green Food Research Institute Harbin150028China Quality&Safety Institute of Agricultural Products Heilongjiang Academy of Agricultural SciencesHarbin150086China

Pea(Pisum sativum L.)is an important source of plant ***,the use of pea flour as a food ingredient is greatly limited due to its strong beany *** this work,we investigated the effects of Lactobacillus rhamnosus L08(*** L08)fermentation on the physico.hemical characteristics,amino acids co.position and volatile flavor co.pounds of pea *** found that *** L08 had the probiotic potential of utilizing pea flour as fermentation *** L08 fermentation significantly increased the co.tent of most amino acids,and total amino acid co.tent increased from 222.18 mg/g at 0 h to 262.35 mg/g at 6 h.A total of 44 volatile co.pounds were identified in pea *** L08 fermentation increased the variety of acids and esters,while reduced the unpleasant flavor such as nonanal,decanal,octanal,1-hexanol and *** emulsifying stability and foam stability of pea flour were slightly enhanced during *** results illustrate that lactic acid bacteria fermentation is a promising approach to eliminate undesirable aroma of pea ***,this study provides theoretical support for the application of peas as high-quality protein source.

关键词： Pea flour Lactic acid bacteria Fermentation Flavor co.ponent Physico.hemical properties