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Food Science and Human Wellness 2024年

作者： Jiaqi Cui Yanfei Hong Wei-Hsien Liu Wen Zhao Xinmei Nan Xuekai Shang Yalan Li Haotian Feng Qiuyue Jiang Weilian Hung Guiying Peng inner mongolia yili industrial group co. Ltd. Department of Immunology and Microbiology School of Life Sciences Beijing University of Chinese Medicine

Many studies have shown that the development of allergic asthma is associated with intestinal microbiota dysbiosis. Based on the gut-lung axis theory， probiotic intervention may be a potential strategy for respiratory diseases. Lacticaseibacillus paracasei K56 was reported to regulate gut microbiota in mice. However， its effect on allergic asthma has not been reported. In this study， we investigated the effect of the K56 on ovalbumin-induced asthma and its possible mechanisms. Our results showed that K56 reduced asthma symptoms and inflammatory cell infiltration in the lung of asthmatic mice. And K56 regulated the differentiation of helper T cells in the lung and intestine. Results from 16S rRNA gene sequencing showed that K56 prophylaxis significantly elevated the richness of Akkermansia and Burkholderia. Meanwhile， K56 abrogated the decrease in the levels of short-chain fatty acids (SCFAs) in the feces. In addition， a rebound in the mRNA expression of the SCFA receptors was observed after K56 prophylaxis. Notably， the regulatory T cell (Treg) frequencies and the FFAR3 levels were positively co.related. These results suggest that K56 co.ld attenuate asthma， possibly by modulating the intestinal microbiota and regulating Treg differentiation through SCFA metabolized. Our study showed that K56 may be used as a probiotic to prevent pulmonary inflammation and dysbiosis of the intestinal microbiota in allergic asthma.

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Validation of Molecular Detection Methods for Gluten Allergens in Infant Formula

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Asian Agricultural Research 2024年第1期16卷 29-31,36页

作者： Shuhuan ZHAO Yunxia WANG Cuizhi LI inner mongolia yili industrial group co. Ltd.Inner MongoliaHohhot 010110China

[Objectives]To verify the specificity,sensitivity,precision and negative-positive deviation of the foodproof gluten co.ponent de-tection kit for the detection of gluten allergens in milk powder matrix,and to establish a real-time fluorescent PCR legal method for the detec-tion of gluten allergens in milk powder.[Methods]The specificity,sensitivity,precision and negative-positive deviation of the detection method of foodproof gluten co.ponent detection kit(PCR-probe method)were verified by artificially adding different co.centrations of wheat bran and extracting sample DNA by kit method,and applied to sample detection.[Results] The specific detection results of two kinds of milk powder with wheat bran and buckwheat added showed that the foodproof gluten co.ponent detection kit(PCR-probe method)had good speci-ficity for wheat *** results of artificially added wheat bran positive samples showed that the false positive rate and false negative rate of the kit in the milk powder matrix were O,and the sensitivity and precision were high.[co.clusions]The kit is simple to operate and has high accuracy,which is suitable for the detection of gluten allergen co.ponents in milk powder.

关键词： Real-time fluorescent PCR Gluten Allergen Infant formula

Application of Next Generation Sequencing for Rapid Identification of Lactic Acid Bacteria

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Asian Agricultural Research 2024年第4期16卷 27-32页

作者： Xiaxia HOU Yunxia WANG Shuhuan ZHAO Hongbing JIA Cuizhi LI inner mongolia yili industrial group co. Ltd.Hohhot 010110China

The rapid identification of lactic acid bacteria,which are essential microorganisms in the food industry,is of great significance for industrial *** identification of lactic acid bacteria traditionally relies on the isolation and identification of pure *** this method is well-established and widely used,it is not without *** subjective judgment inherent in the isolation and purification process introduces potential for error,and the inco.plete nature of the isolation process can result in the loss of valuable *** advent of next generation sequencing has provided a novel approach to the rapid identification of lactic acid *** technology offers several advantages,including rapidity,accuracy,high throughput,and low *** generation sequencing represents a significant advancement in the field of DNA *** ability to rapidly and accurately identify lactic acid bacteria strains in samples with insufficient information or in the presence of multiple lactic acid bacteria sets it apart as a valuable *** application of this technology not only circumvents the potential errors inherent in the traditional method but also provides a robust foundation for the expeditious identification of lactic acid bacteria strains and the authentication of bacterial powder in industrial *** paper co.mences with an overview of traditional and molecular biology methods for the identification of lactic acid *** each method has its own advantages,they are not without limitations in practical ***,the paper provides an introduction of the principle,process,advantages,and disadvantages of next generation sequencing,and also details its application in strain identification and rapid identification of lactic acid *** objective of this study is to provide a co.prehensive and reliable basis for the rapid identification of industrial lactic acid bacteria strains and the authen

关键词： Lactic acid bacteria Rapid identification Next generation sequencing

Research Progress of Microplastics Pollution in Environment

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Asian Agricultural Research 2024年第4期16卷 19-22页

作者： Peng WAN Zhen ZHAO Guoyan WEN Yunshuang FU Cuizhi LI Xiaoli WU inner mongolia yili industrial group co. Ltd.Hohhot 010010China

Microplastics pollution has beco.e one of the focuses of global environmental science *** include micro plastic particles and nano-plastic particles,which co.e from the deco.position of plastic products,the release of microfibers and the industrial process of plastic *** distribution of microplastics in water,soil and atmosphere is summarized,and the widespread existence of microplastics in different environmental media is *** paper also summarizes the potential impact of microplastics on eco.ystems and organisms,and pays attention to the transmission and accumulation of microplastics in the food chain,as well as its potential threat to human ***,the paper discusses the methods and technologies of microplastics treatment and monitoring at present,and puts forward the direction of further research on microplastics pollution in order to formulate more effective management and mitigation strategies.

关键词： Microplastics Environment Pollution

Research Progress of Detection Methods for Microplastics

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Asian Agricultural Research 2024年第3期16卷 29-32页

作者： Peng WAN Zhen ZHAO Guoyan WEN Yunshuang FU Cuizhi LI Xiaoli WU inner mongolia yili industrial group co. Ltd.Hohhot 010010China

Microplastics are plastic particles or fibers with a diameter of less than 5 mm,and they widely exist in the environment and pose potential risks to the eco.ystem and human *** detection can provide basic data for formulating effective environmental protection *** this paper,the physical,chemical and biological detection methods of microplastics are reviewed,and the advantages and disadvantages of different methods are *** problems and challenges enco.ntered in microplastics detection are analyzed,and the future research is discussed.

关键词： Microplastics Detection method Research progress

Effect of normal and strict anaerobic fermentation on physico.hemical quality and metabolomics of yogurt

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Food Bioscience 2022年第2期46卷 356-365页

作者： Ruixue Ding Mohan Li Yiting Zou Yiran Wang Chunyue Yan Haibing Zhang Rina Wu Junrui Wu co.lege of Food Science Shenyang Agricultural UniversityShenyang110866PR China inner mongolia yili industrial group co.pany Limited Hohhot010080PR China

Generally,yogurts on the market are usually anaerobic fermentation,but oxygen can be still dissolved in the production process,which is actually not strict *** study explored the multiple quality indicators and the metabolomic analysis of yogurts fermented by Lactobacillus bulgaricus and Streptoco.cus thermophilus using two fermentation methods,namely,strict anaerobic and normal *** changes in flavor substances in yogurts,including 17 free amino acids,17 fatty acids,and 7 response value,were detected using principal co.ponent and multi factor *** on the results of the co.parisons between basic physical and chemical indicators,strict anaerobic fermentation increased the growth and acidification of the two bacteria,the response value of sourness,umami and *** anaerobic fermentation increased the free amino acid(Fold change=1.53)and short chain fatty acid levels(Fold change=1.73),and reduced the polyunsaturated fatty acid(Fold change=0.51)***,metabolomic analysis based on UPLC-Triple TOF-MS/MS showed that 92 differential metabolites were screened;45 differential metabolites were increased and 47 were *** results revealed that strict anaerobic fermentation was beneficial to metabolic changes and nutritional indicators of yogurt,which may be as indicators of valuable reference to improve dairy fermentation process.

关键词： Yogurt Anaerobic fermentation Metabolomic Free amino acid Fatty acid UPLC-Triple TOF-MS/MS

Lacticaseibacillus paracasei K56 inhibits lipid accumulation in adipocytes by promoting lipolysis

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Food Science and Human Wellness 2023年

作者： Silu Wang Jinxing Li Wei-Hsien Liu Niya Li Huijing Liang Weilian Hung Qiuyue Jiang Ruyue Cheng Xi Shen Fang He Department of Nutrition and Food Hygiene West China School of Public Health and West China Fourth Hospital Sichuan University inner mongolia yili industrial group co. Ltd

Probiotics crosstalk immunity to improve host glyco.ipid metabolism， which is a new strategy for obesity. This study aimed to explore the functions of Lacticaseibacillus paracasei K56 (K56)， in regulating the lipid metabolism of adipocytes and its immune regulation *** co.cultured RAW264.7 macrophages with K56， and the K56-stimulated RAW264.7-co.ditioned medium (K56-CM) was co.lected and treated with 3T3-L1 pre-adipocytes. The expression of lipid metabolism-related markers of adipocytes， and the co.tent of cytokines in CMs were detected. The results demonstrated that K56-CM promoted the expression of peroxisome proliferator-activated receptor-γ (PPAR-γ)， CCAAT/enhancer binding protein α (C/EBPα)， and other adipogenesis-related markers， which resulted in the upregulation of lipolysis markers， such as hormone-sensitive lipase (HSL)， adipose triglyceride lipase (ATGL). The activation of lipolysis enhanced the expression of fatty acid β-oxidation-related and mitochondrial biogenesis-related markers and reduced lipid accumulation in adipocytes. The glyco.ipid metabolism pattern of K56 may be due to its immunomodulatory characteristics， which stimulated macrophages to secrete fewer TNF-α， thereby promoting the expression of lipolysis-related markers， and TNF-α synergized with lipase to promote lipolysis. It has not been reported that Lacticaseibacillus paracasei modulated lipid metabolism via the lipolysis pathway， which suggested that K56 may regulate glyco.ipid metabolism of the host by maintaining immune homeostasis.

关键词：

Inhibitory Effect of Lactic Acid Bacteria on Salmonella

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Asian Agricultural Research 2022年第5期14卷 27-30,35页

作者： Yunxia WANG Lijuan JING Shuhuan ZHAO Lijun LIU Cuizhi LI Zhiyong LU Zhijun LI Quality Management Department Inner Mongolia Yili Industrial Group Co. Ltd. Hohhot 010110 China Quality Management Department of Yogurt Division Inner Mongolia Yili Industrial Group Co. Ltd. Hohhot 010110 China

[Objectives]To explore the inhibitory effect of lactic acid bacteria(LAB)on the growth of Salmonella.[Methods]The inhibitory effect of lactic acid bacteria on Salmonella was investigated by Oxford cup agar diffusion test from the aspects of lactic acid bacteria body,metabolites,pH,thermal stability and acid resistance.[Results]The growth of Salmonella was disturbed by the inhibition of lactic acid *** mechanism of inhibitory effect may be:simple low pH and lactic acid bacteria have no inhibitory effect on Salmonella;however,there are active substances in the metabolites of lactic acid bacteria,which have high thermal stability and a wide range of acid resistance,and are effective substances for inhibiting the growth of Salmonella.[co.clusions]This study is helpful for screening more natural preservative substances,reducing the co.tamination rate of Salmonella to food,and is expected to provide a theoretical basis for the preservation and preservation of food.

关键词： Lactic acid bacteria(LAB) Fermented product Salmonella Inhibitory effect

Analysis and Evaluation of Uncertainty in the Determination of Organic Pollutants

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Asian Agricultural Research 2022年第6期14卷 43-46页

作者： Shuhuan ZHAO Yunxia WANG Lijun LIU Cuizhi LI Zhiyong LU Zhijun LI Quality Management Department Inner Mongolia Yili Industrial Group Co. Ltd. Hohhot 010110 China Quality Management Department of Yogurt Division Inner Mongolia Yili Industrial Group Co. Ltd. Hohhot 010110 China

[Objectives]To determine the co.tent of organic pollutants by CALUX bioassay.[Methods]Acco.ding to JJF 1059.1-2012 Technical Specification for Evaluation and Expression of Uncertainty in Measurement,the determination results of organic pollutants were analyzed,various sources of uncertainty that may be introduced in the detection process were analyzed,and the mathematical model of uncertainty was *** A and B evaluation methods were used to calculate the co.ponents of uncertainty and extended uncertainty.[Results]In the range of 95%co.fidence interval,the determination result of organic pollutants was 6.59 pg/g fat,and the extended uncertainty was 0.0764 pg/g fat,so the determination results of organic pollutants co.ld be expressed as(6.59±0.0764)pg/g fat(k=2).[co.clusions]This study can provide a basis for more accurate determination of organic pollutants.

关键词： Uncertainty Organic pollutants CALUX bioassay

Simultaneous Determination of co.tent of VK_(1) and VK_(2) in Milk and Dairy Products by High Performance Liquid Chromatography

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Asian Agricultural Research 2022年第7期14卷 63-68,71页

作者： Huili GONG Guoxia DUAN Lijun LIU Shuo TANG Cuizhi LI Zhiyong LU inner mongolia yili industrial group co.Ltd. Hohhot 010110China

[Objectives]To make simultaneous determination of vitamin K_(1)(VK_(1))and vitamin K_(2)(VK_(2),mainly MK-4,MK-7 and MK-9)in milk and dairy products.[Methods]A high performance liquid chromatographic method was developed for the simultaneous determination of vitamin K_(1)(VK_(1))and vitamin K_(2)(VK_(2),mainly MK-4,MK-7 and MK-9)in milk and dairy *** enzymatic digestion,the samples were extracted with hexane for VK_(1),MK-4,MK-7 and MK-9,subjected to gradient elution at excitation wavelength 243 nm and emission wavelength 430 nm,detected by high performance liquid chromatography with fluorescence detector and quantified by external standard method.[Results]The linearity of VK_(1),MK-4,MK-7 and MK-9 was good in the co.centration range of 2.5-1000 ng/mL with the co.relation co.fficients greater than 0.999;The relative standard deviations(RSD)of VK_(1),MK-4,MK-7 and MK-9 in milk powder,liquid milk and yogurt were 1.32%-5.05%,1.10%-2.48% and 2.20%-3.47%,respectively;the reco.ery rates of VK_(1),MK-4,MK-7 and MK-9 at different levels in milk powder,liquid milk and yogurt were 81.1%-108%,81.8%-103%and 82.1%-99.2%,respectively.[co.clusions]The method is rapid,accurate,reproducible and capable of simultaneous determination of VK_(1),MK-4,MK-7 and MK-9.

关键词： High performance liquid chromatography(HPLC) Vitamin K_(1) Vitamin K_(2) MK-4 MK-7 MK-9