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Anticancer effect of Psidium guajava(Guava) leaf extracts against colorectal cancer through inhibition of angiogenesis

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Asian Pacific Journal of Tropical Biomedicine 2020年第7期10卷 293-307页

作者： Bronwyn Lok Doblin Sandai Hussein M.Baharetha Mansoureh Nazari V Muhammad Asif Chu Shan Tan AMS Abdul Majid EMAN Laboratory Discipline of PharmacologySchool of Pharmaceutical SciencesUniversiti Sains MalaysiaPenangMalaysia infectomics cluster Advance Medical and Dental InstituteUniversiti Sains MalaysiaPenangMalaysia Department of Pharmacy College of Medicine and Health SciencesHadhramout UniversityHadhramoutYemen Department of Pharmacology Faculty of Pharmaceutical SciencesGovernment College University FaisalabadFaisalabadPakistan Discipline of Pharmacology School of Pharmaceutical SciencesUniversiti Sains MalaysiaPenangMalaysia ACRF Department of Cancer Biology and Therapeutics John Curtin School of Medical ResearchAustralian National UniversityCanberraAustralia

Objective:To evaluate the anti-angiogenic and anticancer activities of Psidium guajava leaf extracts against angiogenesis-dependent colorectal ***:Three extracts were produced using distilled water,ethanol,and n-hexane as *** extracts were physically characterised through gas chromatography–mass spectrometry,ultraviolet–visible spectroscopy,and Fourier transform infrared *** antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl,total phenolic content,and total flavonoid content *** assess their anti-angiogenic activity,cell viability and rat aortic ring assays were conducted,while cell migration,tube formation,colony formation,and VEGF ELISA assays were conducted to elucidate their effects on different aspects of *** docking was used to assess the antiangiogenic potential of some possible compounds in the *** spheroid assay was used to assess the extracts’potential as a treatment for colorectal ***:The ethanol extract showed the best antioxidant *** distilled water and ethanol extracts exhibited more inhibitory activity against ***926 cell viability and aortic ring microvessel *** addition,the ethanol extract performed significantly better than the distilled water extract against cell migration and colony formation,and VEGF expression of the cells was suppressed by the ethanol *** the distilled water and ethanol extracts showed significant inhibitory effect on ***926 tube formation and tumour spheroids consisting of ***926 and HCT116 *** ethanol extract containedβ-caryophyllene andβ-elemene by phytochemical analysis and subsequent docking studies,which may contribute to its anti-angiogenic ***:The ethanol extract of Psidium guajava has potential in the treatment of colorectal cancer through the inhibition of angiogenesis.

关键词： Angiogenesis Antioxidant Colorectal cancer Psidium guajava VEGF

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Naringenin prevents experimental liver fibrosis by blocking TGFβ-Smad3 and JNK-Smad3 pathways

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World Journal of Gastroenterology 2017年第24期23卷 4354-4368页

作者： Erika Hernández-Aquino Natanael Zarco Sael Casas-Grajales Erika Ramos-Tovar Rosa E Flores-Beltrán Jonathan Arauz Mineko Shibayama Liliana Favari Víctor Tsutsumi José Segovia Pablo Muriel Laboratory of Experimental Hepatology Department of PharmacologyCinvestav-IPNApartado Postal 14-740Mexico City Department of Physiology Biophysics and NeurosciencesCinvestav-IPNApartado Postal 14-740Mexico City Department of Pharmacology School of MedicineAutonomous University of Baja CaliforniaMexicaliApartado Postal 21100 Department of infectomics and Molecular Pathogenesis Cinvestav-IPNApartado Postal 14-740Mexico City

To study the molecular mechanisms involved in the hepatoprotective effects of naringenin (NAR) on carbon tetrachloride (CCl4)-induced liver fibrosis. METHODSThirty-two male Wistar rats (120-150 g) were randomly divided into four groups: (1) a control group (n = 8) that received 0.7% carboxy methyl-cellulose (NAR vehicle) 1 mL/daily p.o.; (2) a CCl4 group (n = 8) that received 400 mg of CCl4/kg body weight i.p. 3 times a week for 8 wk; (3) a CCl4 + NAR (n = 8) group that received 400 mg of CCl4/kg body weight i.p. 3 times a week for 8 wk and 100 mg of NAR/kg body weight daily for 8 wk p.o.; and (4) an NAR group (n = 8) that received 100 mg of NAR/kg body weight daily for 8 wk p.o. After the experimental period, animals were sacrificed under ketamine and xylazine anesthesia. Liver damage markers such as alanine aminotransferase (ALT), alkaline phosphatase (AP), γ-glutamyl transpeptidase (γ-GTP), reduced glutathione (GSH), glycogen content, lipid peroxidation (LPO) and collagen content were measured. The enzymatic activity of glutathione peroxidase (GPx) was assessed. Liver histopathology was performed utilizing Masson’s trichrome and hematoxylin-eosin stains. Zymography assays for MMP-9 and MMP-2 were carried out. Hepatic TGF-β, α-SMA, CTGF, Col-I, MMP-13, NF-κB, IL-1, IL-10, Smad7, Smad3, pSmad3 and pJNK proteins were detected via western blot. RESULTSNAR administration prevented increases in ALT, AP, γ-GTP, and GPx enzymatic activity; depletion of GSH and glycogen; and increases in LPO and collagen produced by chronic CCl4 intoxication (P 4. Although zymography assays showed that CCl4 produced an increase in MMP-9 and MMP-2 gelatinase activity; interestingly, NAR administration was associated with normal MMP-9 and MMP-2 activity (P < 0.05). The anti-inflammatory, antine

关键词： Fibrosis Transforming growth factor-β Naringenin pSmad3 Smad3 JNK Nuclear factor kappa Carbon tetrachloride

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