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Advances in genotypic antimicrobialresistance testing: a comprehensive review

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Science China Life Sciences 2025年第1期68卷 130-143页

作者： Boheng Duan Xianjun Zeng Junping Peng Huan Kui College of Nanchang University Department of Imaging The Second Affiliated Hospital of Nanchang University NHC Key Laboratory of Systems Biology of Pathogens National Institute of Pathogen BiologyChinese Academy of Medical Sciences & Peking Union Medical College Key Laboratory of Respiratory disease Pathogenomics Chinese Academy of Medical Sciences & Peking Union Medical College Key Laboratory of Pathogen infection Prevention and Control (Ministry of Education) State Key Laboratory of Respiratory Health and MultimorbidityNational Institute of Pathogen BiologyChinese Academy of Medical Sciences & Peking Union Medical College

Antimicrobial resistance(AMR) represents a substantial threat to global public health, complicating the treatment of common infections and leading to prolonged illness and escalated healthcare expenses. To effectively combat AMR, timely and accurate detection is crucial for AMR surveillance and individual-based therapy. Phenotypic antibiotic resistance testing(AST) has long been considered the gold standard in clinical applications, serving as the foundation for clinical AMR diagnosis and optimized therapy. It has significantly contributed to ensuring patients′ health and the development of novel antimicrobials. Despite advancements in automated culture-based AST technologies,inherent limitations impede the widespread use of phenotypic AST in AMR surveillance. Genotypic AST technologies offer a promising alternative option, exhibiting advantages of rapidity, high sensitivity, and specificity. With the continuous advancement and expanding applications of genotypic AST technologies, such as microfluidics, mass spectrometry, and high-resolution melting curve analysis, new vigor has been injected into the development and clinical implementation of genotypic AST technologies. In this narrative review, we discuss the principles, applications, and advancements of emerging genotypic AST methods in clinical settings. The comprehensive review aims to highlight the significant scientific potential of emerging genotypic AST technologies in clinical AMR diagnosis, providing insights to enhance existing methods and explore novel approaches.

关键词： antimicrobial resistance (AMR) genotypic antibiotic resistance testing (AST) AST technologies review

Intestinal detoxification time of hand-foot-and-mouth disease in children with EV71 infection and the related factors

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World Journal of Pediatrics 2015年第4期11卷 380-385页

作者： Shu Teng Yi Wei Shi-Yong Zhao Xian-Yao Lin Qi-Min Shao Juan Wang Department of Pediatric infection Hangzhou Children's HospitalHangzhouChina

Background:Hand-foot-and-mouth disease(HFMD)is a common pediatric infectious disease caused by a variety of intestinal *** 71(EV71)is the primary pathogen that might cause severe symptoms and even death in children with *** study aimed to investigate the intestinal detoxification time of HFMD children with EV71 infection and its related ***:Sixty-five HFMD children with EV71 infection were followed *** stool samples were collected once every 4 to 7 *** nucleic acids were detected byfl uorescent polymerase chain reaction until the results became *** positive rates of viral nucleic acids were analyzed by the Kaplan-Meier *** Log-rank test and Cox-Mantel test were used to analyze factors affecting the HFMD children with EV71 ***:On the 2nd,4th,6th and 10th week,the positive rates of viral nucleic acids in stool samples of the 65 children were 94.6%,48.1%,17.2%and 0,*** analysis showed that the intestinal detoxification time of the children were related to gender,pre-admission disease course,severity of disease,and use of steroids or gamma globulin(Pdisease was an independent factor affecting the intestinal detoxification time(Pinfection was 10 *** severity of disease was an important factor affecting the intestinal detoxification time of HFMD children with EV71 *** HFMD children with EV71 infection had a longer intestinal detoxification time.

关键词： detoxification time enterovirus 71 hand-foot-and-mouth disease relative factor

Three-dimensional cell culture models for investigating human viruses

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Virologica Sinica 2016年第5期31卷 363-379页

作者： Bing He Guomin Chen Yi Zeng State Key Laboratory of infection disease Prevention and Control National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention

Three-dimensional(3D) culture models are physiologically relevant, as they provide reproducible results, experimental flexibility and can be adapted for high-throughput experiments. Moreover,these models bridge the gap between traditional two-dimensional(2D) monolayer cultures and animal models. 3D culture systems have significantly advanced basic cell science and tissue engineering, especially in the fields of cell biology and physiology, stem cell research, regenerative medicine, cancer research, drug discovery, and gene and protein expression studies. In addition,3D models can provide unique insight into bacteriology, virology, parasitology and host-pathogen interactions. This review summarizes and analyzes recent progress in human virological research with 3D cell culture models. We discuss viral growth, replication, proliferation, infection, virus-host interactions and antiviral drugs in 3D culture models.

关键词： three-dimensional (3D) cell culture models scaffolds human viruses

Influenza virus H1N1 induced apoptosis of mouse astrocytes and the effect on protein expression

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Asian Pacific Journal of Tropical Medicine 2014年第7期7卷 572-575页

作者： Xu-Dong Pei Yu-Feng Zhai Huai-Hong Zhang Department of infection and Liver disease Nanyang Central Hospital

Objective:To investigate the effects of influenza A virus H1N1 infection on the proliferation and apoptosis of mouse astrocytes cells and its protein ***:After mouse astrocytes was infected with purified influenza A virus H1N1 in vitro,viral integration and replication status of the cells were detected by RT-PCR assay,cell proliferation and apoptosis was determined by MTT method and flow cytometry,*** protein expression was delected by Western ***:Agarose gel electrophoresis showed H1N1 virus can infect astrocytes and can be *** staining showed H1N1 virus infection can inhibit the proliferation of mouse astrocytes,which makes cell viability decreased *** cytometry showed that the proportion of Annein V staining positive vascular endothelial cells in the influenza A virus group was significantly higher than that in the control *** blot analysis showed after24 h and 32 h of infection,there were cells caspase-3 protein and the expression of its active form(lysed caspase-3 protein)*** proportion of Bax/Bcl-2 also ***:Influenza A virus can infect human vascular endothelial cells and proliferation and it can induce apoptosis of endothelial cells.

关键词： Influenze virus H1N1 Apoptosis

Clostridium difficile infection and inflammatory bowel disease:Understanding the evolving relationship

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World Journal of Gastroenterology 2010年第39期16卷 4892-4904页

作者： Udayakumar Navaneethan Preethi GK Venkatesh Bo Shen Victor W.Fazio Center for Inflammatory Bowel disease Department of GastroenterologyDigestive Disease Institutethe Cleveland Clinic Foundation

Clostridium difficile(***)infection(CDI)is the leading identifiable cause of antibiotic-associated *** there is an alarming trend of increasing incidence and severity of CDI in the united States and Europe,superimposed CDI in patients with inflammatory bowel disease(IBD)has drawn considerable attention in the gastrointestinal *** majority of IBD patients appear to contract CDI as *** affects disease course of IBD in several ways,including triggering disease flares,sustaining activity,and in some cases,acting as an"innocent"*** its wide spectrum of presentations,CDI has been reported to be associated with a longer duration of hospitalization and a higher mortality in IBD *** patients with restorative proctocolectomy or with diverting ileostomy are not immune to CDI of the small bowel or ileal *** immunomodulator or corticosteroid therapy for IBD should be continued in patients with superimposed CDI is *** appears that more adverse outcomes was observed among patients treated by a combination of immunomodulators and antibiotics than those treated by antibiotics *** use of biologic agents does not appear to increase the risk of acquisition of *** CDI in the setting of underlying IBD,vancomycin appears to be more efficacious than *** controlled trials are required to clearly define the appropriate management for CDI in patients with IBD.

关键词： Clostridium difficile Inflammatory bowel disease Antibiotics Colectomy

Interaction Between the Neglected Tropical disease Human Schistosomiasis and HCV infection in Egypt:a Puzzling Relationship

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Journal of Clinical and Translational Hepatology 2014年第2期2卷 134-141页

作者： Mahmoud M. Bahgat Immunology and Infectious diseases Group Therapeutic Chemistry Department the Centre of Excellence for Advanced Sciences the National Research Centre Dokki Cairo Egypt Research Group of Biomarkers for infection and Immunity Institute of Experimental Infection Research TWINCORE Centre for Experimental and Clinical Infection Research Hannover Germany

Egypt has the highest prevalence of chronic hepatitis C virus (HCV) infection and seropositivity worldwide, and it has been proposed that this enhanced susceptibility to HCV is related to coinfection with schistosomiasis. Although currently, there are no studies regarding the actual prevalence of both human schistosomiasis and schistosomiasis/HCV coinfection evi-dences strongly support that eliminating human schistoso-miasis from Egypt is necessary to reduce both HCV prevalence and liver pathology. The present review highlights the significant impact of the neglected tropical disease human schistosomiasis on both susceptibility of Egyptians to HCV coinfection, severity of the resulting liver pathology, and poor response to antiviral therapy. The immune evasion mechan-isms exerted by the HCV-NS3/4A protease domain, and the possible impact of immune evasion mechanisms exerted by proteases of larval, worm and egg stages of the parasite Schistosoma on human susceptibility to HCV infection are discussed. In addition, schistosome immune evasion mechanisms may include immunosuppression that in turn prevents clearance of HCV viremia and leads to relapsing HCV infection and severe liver pathology. I propose the generation of a replicon system from the most prevailing genotype (HCV-4a) in Egypt and establishing its replication on hepatoplas-toma or immune cells in presence of bilharzial antigens. Finally, the use of a humanized small animal model that can acquire both HCV and S. mansoni infections will be important to further understand in real time the impact of coinfection on both the immune system and liver pathology.

关键词： Egypt Neglected tropical disease Human schistosomiasis HCV Coinfection Immune evasion mechanisms HCV protease Schistosome pro-teases

Therapeutic Potential of Anti-HIV RNA-loaded Exosomes

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Biomedical and Environmental Sciences 2018年第3期31卷 215-226页

作者： QIU Yong MA Jing ZENG Yi College of Life Science and Bioengineering Beijing University of Technology State Key Laboratory of infection disease Prevention and Control National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention

Highly active antiretroviral therapy（HAART）is used globally and has significantly reduced the morbidity,mortality,and transmission of human immunodeficiency virus（HIV）-related ***,the attendant shortcomings such as toxic side effects,necessity of life-long therapy that is expensive, and inefficiency in eradicating latent viral infections have greatly limited the application of HAART.

关键词： HIV Therapeutic Potential of Anti-HIV RNA-loaded Exosomes RNA

Epidemiological Investigation of Human Papillomavirus infection in Men Attending a Sexually Transmitted disease Clinic in Hangzhou Area

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Biomedical and Environmental Sciences 2006年第2期19卷 153-157页

作者： XU TANG AI-E XU XIAO-PING DONG XIU-KUN SUN HONG SHEN JI-FENG LIU Department of Dermatology the 3rd Hospital of Hangzhou Hangzhou 310009 Zhejiang China Prion s Laboratory National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 100052 China

Objective To investigate the epidemiological characteristics of human papillomavirus （HPV） infection in men attending a sexually transmitted diseases （STD） clinic in Hangzhou area. Methods Male subjects （n=375） aged 18-70 years, attending the STD clinic were recruited. Urethral swabs were assessed for HPV DNA using polymerase chain reaction （PCR） with the consensus primers MY09/11. HPV genotypes of positive PCR products were determined by restriction fragment length polymorphisms and direct sequence analysis. Results Of the 375 swabs collected, 305 （81.3%） yielded sufficient DNA for the subsequent HPV analysis. Among the 305 subjects, the prevalence of HPV was 13.8%. Nononcogenic HPV types were found in 8.5% （26/305） of subjects, oncogenic types in 4.3% （13/305）, and multiple types in 1.0% （3/305）. The prevalence of HPV infection was higher in subjects from urban area than in those from rural area （P〈0.05）. The prevalence was also higher in those who received fewer years of education （P〈0.05） and those who had more sex partners （P〈0.05）. Conclusions HPV infection among men at high risk is not uncommon. The detection rate of HPV DNA is significantly related to some sociodemographic factors, such as residence, educational level and the number of sex partners.

关键词： Human papillomavirus Men Sexually transmitted diseases

Evolution of Influenza A H7N9 Virus with an Emphasis on Gene Constellation

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Journal of Genetics and Genomics 2014年第1期41卷 3-6页

作者： Zifeng Yang Runfeng Li Tianyu Zhang State Key Laboratory of Respiratory disease The First Affiliated HospitalGuangzhou Medical University Guangzhou Institute of Respiratory disease National Clinical Research Center for Respiratory disease Center for infection and Immunity Guangzhou Institute of Biomedicine and Health Chinese Academy of Sciences

The H7 subtype avian influenza viruses, including H7N2, HTN3 and HTN7, have posed a public health threat worldwide. Except one H7N7 fatal case in the Netherlands in 2003, the other H7 human cases have resulted in self... 详细信息

关键词： In gene Evolution of Influenza A H7N9 Virus with an Emphasis on Gene Constellation