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Mercury in sediments and vegetation in a moderately contaminated salt marsh (Tagus Estuary, Portugal)

Journal of Environmental Sciences 2010年第8期22卷 1151-1157页

作者： Joao Canário Carlos Vale Laurier Poissant Marta Nogueira Martin Pilote Vasco Branco inrb I.P./IPIMAR Av. Brasília Environment Canada Science and Technology Branch

Depth variations of total mercury （Hg） and methylmercury （MeHg） concentrations were studied in cores from non-colonized sediments, sediments colonized by Halimione portulacoides, Sarcocornia fruticosa and Spartina maritima and belowground biomass, in a moderately contaminated salt marsh （Tagus Estuary, Portugal）. Concentrations in belowground biomass exceeded up to 3 （Hg） and 15 （MeHg） times the levels in sediments, and up to 198 （Hg） and 308 （MeHg） times those found in aboveground parts. Methylmercury in colonized sediments reached 3% of the total Hg, 50 times above the maximum values found in non-colonized sediments. The absence of correlations between total Hg concentrations in sediments and the corresponding MeHg levels suggested that methylation was only dependent on the environmental and microbiological factors. The analysis of belowground biomass at high-depth resolution （2 cm） provided evidence that Hg and MeHg were actively absorbed from sediments, with higher enrichment factors at layers where higher microbial activity was probably occurring. The results obtained in this study indicated that the biotransformation of Hg to the toxic MeHg could increase the toxicity of plant-colonized sediments.

关键词： mercury methylmercury salt marshes Tagus Estuary Portugal

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Analysis of genetic relationship among Arbutus unedo L. genotypes using RAPD and SSR markers

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Journal of Forestry Research 2013年第2期24卷 227-236页

作者： Filomena Gomes Rita Costa Maria M. Ribeiro Elisa Figueiredo Jorge M. Canhoto CERNAS Departamento Florestal Escola Superior Agrária Coimbra inrb Instituto Nacional de Recursos Biológicos Departamento de Recursos Naturais e Desenvolvimento Sustentável Escola Superior Agrária Centre of Functional Ecology Department of Life Sciences University of Coimbra

The strawberry tree （Arbutus unedo L.） is an underutilized, drought tolerant, fire resistant species with a south western distribution in Europe, and with ecological and putative socio-economical impact in Portugal and Mediterranean countries. Our aim was to develop an appropriate set of molecular markers to enable genetic diversity to be assessed and to fingerprint Arbutus unedo genotypes for breeding and conservation purposes in Portugal. Twenty-seven trees from a broad geographic range were screened with 20 random amplified polymorphic DNA （RAPD primers） and 11 microsatellite markers （SSR）. The RAPDs generated 124 bands, 57.3% of which were polymorphic, with an expected heterozygosity of 27%. We cross-amplified 11 SSR primers developed for Vaccinium spp., and 5 were found to be polymorphic in A. unedo, with 75% of expected heterozygosity, a number of alleles of 11.6, a null allele frequency of 7.6% and a polymorphic information content of 71%. Although the SSRs were more polymorphic and informative than the RAPDs, both markers displayed high genetic variability with the gathered data. No geographic pattern was observed in the genetic variation distribution based on both marker systems, and the lack of correlation between genetic and geographical matrices was confirmed by Mantel tests. Likely, no correlation was found between pairwise SSR and RAPD band-sharing matrices. These results and their implications on A. unedo breeding and conservation programs are discussed.

关键词： Ericaceae fingerprinting geographic pattern molecular markers strawberry tree.

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The road towards sustainable control of schistosomiasis in the Democratic Republic of Congo:Pre-assessment of staff performance and material resources in endemic regions

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Asian Pacific Journal of Tropical Biomedicine 2017年第4期7卷 275-279页

作者： Sylvie Linsuke Liliane Mpabanzi Sabin Nundu Faustin Mukunda Pascal Lutumba Katja Polman National Institute of Biomedical Research(inrb) Department of Epidemiology Biomedical Sciences Department Institute of Tropical Medicine National Control Program against Schistosomiasis and Intestinal Parasites(PNLB/PI) Ministry of Health Department of Tropical Medicine Faculty of MedicineUniversity of Kinshasa

Objective: To improve knowledge and practice of health staff as well as the availability of material resources for diagnosis and management of schistosomiasis in two endemic provinces of DRC(Kinshasa and Bas-Congo).Methods: Structured interviews were performed using questionnaires with staff from 35 healthcare facilities in 9 health zones(HZ) of Kinshasa and 2 HZ in ***: Schistosomiasis was reported to be present in all the included *** staff knew the most important symptoms of schistosomiasis, but advanced symptoms were more accurately reported in *** of symptoms related to schistosomiasis such as anemia(P = 0.0 115) and pollakiuria(P = 0.0 260) was statistically different in both two ***-Katz technique and urine filtration were unavailable in both *** diagnosis was mostly performed using the direct smear *** was available in 70% of the health facilities, all situated in *** and treatment mostly relied on symptoms and cost more in urban area than in ***: Though knowledge on schistosomiasis among health staff appears sufficient, substantial efforts still must be made to improve the availability of diagnostic tools and treatment in the health facilities in DRC.

关键词： Schistosomiasis Control Performance staff and material resources Democratic Republic of Congo

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