检索结果-南通市图书馆

Biomedical and Environmental Sciences 2009年第1期22卷 32-39页

作者： SHAO-LING WANG YING LI YING WEN YAN-FENG CHEN LI-XIN NA SONG-TAO LI CHANG-HAO SUN Department of Nutrition and Food hygiene Harbin Medical University No. 157 Baojian Road Harbin 150081 Heilongjiang China

Objective To investigate the attenuating effect of curcumin, an anti-inflammatory compound derived from dietary spice turmeric （Curcuma longa） on the pro-inflammatory insulin-resistant state in 3T3-L1 adipocytes. Methods Glucose uptake rate was determined with the [3H] 2-deoxyglucose uptake method. Expressions of tumor necrosis factor-α （TNF-α） and interleukin-6 （IL-6） were measured by quantitative RT-PCR analysis and ELISA. Nuclear transcription factor kappaB p65 （NF-r,B p65） and mitogen-activated protein kinase （MAPKs） were detected by Western blot assay. Results The basal glucose uptake was not altered, and curcumin increased the insulin-stimulated glucose uptake in 3T3-L1 cells. Curcumin suppressed the transcription and secretion of TNF-α and IL-6 induced by palmitate in a concentration-dependent manner. Palmitate induced nuclear translocation of NF-kB. The activities of Jun NH2-terminal kinase ONK）, extracellular signal-regulated kinasel/2 （ERKI/2） and p38MAPK decreased in the presence of curcumin. Moreover, pretreatment with SP600125 （inhibitor of JNK） instead of PD98059 or SB203580 （inhibitor of ERKI/2 or p38MAPK, respectively） decreased the up-regulation of TNF-α induced by palmitate. Conelusion Curcumin reverses palmitate-induced insulin resistance state in 3T3-L1 adipocytes through the NF-kB and JNK pathway.

关键词： Curcumin Insulin resistance Inflammation Adipocyte Free fatty acids

Composition of inflammatory infiltrate and its correlation with HBV/HCV antigen expression

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2008年第25期14卷 4040-4046页

作者： Bozena Walewska-Zielecka Kazimierz Madalinski Joanna Jablonska Paulina Godzik Joanna Cielecka-Kuszyk Bogumila Litwinska Department of Virology National Institute of Public Health National Institute of Hygiene Department of Hepatology and AIDS Warsaw Medical University

AIM： To study the composition of liver inflammatory infiltrate in biopsy material from patients chronically infected with hepatotropic viruses and to evaluate the correlation of inflammatory infiltrate with hepatitis B virus （HBV） and hepatitis C virus （HCV） viral antigen expression in chronic B and C hepatitis. METHODS： The phenotype of inflammatory cells was evaluated by the EnVision system, using a panel of monoclonal antibodies. HBV and HCV antigens were detected with the use of monoclonal anti-HBs, polyclonal anti-HBc and anti-HCV antibodies, respectively. RESULTS： The cellular composition of liver inflammatory infiltrate was similar in the patients with B and C hepatitis：～50%-60% of cells were T helper lymphocytes. Approximately 25% were T cytotoxic lymphocytes; B lymphocytes comprised 15% of inflammatory infiltrate; other cells, including NK, totalled 10%. Expression of HLA antigens paralleled inflammatory activity. Portal lymphadenoplasia was found more often in hepatitis C （54.5%） than in hepatitis B （30.6%）. Expression of HB-cAg was found more often in chronic B hepatitis of moderate or severe activity. Overall inflammatory activity in HBV-infected cases did not correlate with the intensity of HBsAg expression in hepatocytes. Inflammatory infiltrates accompanied the focal expression of HCV antigens. A direct correlation between antigen expression and inflammatory reaction in situ was noted more often in hepatitis C than B. CONCLUSION： Irrespective of the etiology and activity of hepatitis, components of the inflammatory infiltrate in liver were similar. Overall inflammatory activity did not correlate with the expression of HBsAg and HCVAg; HBcAg expression, however, accompanied chronic hepatitis 8 of moderate and severe activity.

关键词： Chronic B and C hepatitis Inflammatoryinfiltrate Lymphoid follicle Lymphadenoplasia

Inhibition of PKB/Akt activity involved in apigenin-induced apoptosis in human gastric carcinoma cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2007年第16期52卷 2226-2232页

作者： YUAN LinHong XIA Wei ZHAO XiuJuan ZHANG XiaoHua ZHANG Ling WU Kun Department of Nutrition and Food hygiene School of Public Health Harbin Medical University Harbin 150081 China Department of Nutrition and Food hygiene School of Public Health and Family MedicineCapital Medical University Beijing100061 China

Apigenin is a flavonoid widely distributed in fruits and vegetables. It possesses growth inhibitory properties against numerous cancer cell lines. However,the molecular mechanism(s) by which api-genin elicits its effects have not been fully elucidated. Here we studied whether apigenin inhibits growth and induces apoptosis in human gastric carcinoma cells. We showed that the flavonoid inhibited growth of the cells and caused apoptosis,as evidenced by DNA Ladder,cleavage of pro-caspase-3 in a time-dependent manner. Induction of apoptosis was dependent on inhibition of the PKB/Akt activity. We found that while apigenin had no effect on the expression of Akt and Bad,it inhibited specific phosphorylation of the two proteins that are associated with pro-survival mechanisms. We propose that this important flavonoid induces apoptosis in gastric cancer cells by inhibiting Akt activity. Since Akt is often activated in cancers,our findings may have clinical implications.

关键词：胃癌细胞凋亡 PKB/Akt 类黄酮分子机制

A New Hapten for Immunoassay of Aldicarb

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2006年第8期17卷 1021-1024页

作者： Yan Feng ZHANG Zhi Xian GAO Qing Min ZHANG Shu Gui DAI College of Environmental Science and Engineering Nankai University Tianjin 300071 Institute of hygiene and Environmental Medicine Tianjin 300050

A new hapten, aldicarb oxime succinic ester （AOSE）, was synthesized for immunoassay of aldicarb. It was conjugated to proteins by active ester method. Polyclonal antibody was raised against AOSE-BSA （bovine serum albumin） conjugate. Enzyme-linked immunosorbent assays （ELISAs） showed that this antiserum had high affinity to aldicarb and can be used for sensitive and selective immunoassay of aldicarb.

关键词： Aldicarb immunoassay hapten analysis.

Integrated nuclear techniques for sedimentation assessment in Latin American region

在线全文

学校读者我要写书评

暂无评论

International Soil and Water Conservation Research 2020年第4期8卷 406-409页

作者： JoséLuis Peralta Vital Reinaldo Honorio Gil Castillo Yanna Llerena Padrón Yusleidy Milagro Cordovi Miranda Naymi Labrada Arevalo Leroy Alonso Pino Environmental Impact Assessment Group Center of Radiation Protection and HygieneLa HabanaZip Code 10600Cuba

The paper shows the novel techniques introduction in the region of Latin America and the Caribbean in order to strengthen the surveillance and monitoring of the sedimentation phenomenon in the surface water *** integrated use of the nuclear techniques allows a more complete assessment of water and land *** the development of a regional project,supported by the International Agency of Atomic Energy,Cuba participates as technical leader for 15 countries,to encourage and implement the integrated application of 3 nuclear techniques(Fallout Radionuclides(137Cs,7Be,210pb),Compound Specific Stable Isotopes(fatty acids of the(e)13C)and the Isotopic Hydrology(18O,2H,3H).The methodology used in the last studies referred to RLA 5076 Project is to apply the integration of these three techniques allows evaluating,in the hydraulic facilities and surface water reservoirs,the negative sedimentation impacts in natural and anthropic process as environmental and social *** techniques evaluate the sedimentation process and its synergy,obtaining since the soil quantification in the landscape,definition the soil origin deposited until the dynamic characterization of the water body as soil *** integrated application of these nuclear techniques in the Project RLA-5076 confirms it as a useful tool to support the decisions makers in the definition of the national strategies and programs related to the land and the water resources *** the support of the IAEA projects in the Latin American and Caribbean region,has been evidenced an important increment in the use of these novel techniques.

关键词： Nuclear technique Sedimentation Water reservoir Soil Land Hydric resources

Synergistic Effect of Zinc and Vitamin A on T Cell Functions

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2007年第2期20卷 131-134页

作者： TING-XIN LI YUN LI Department of Nutrition and Food hygiene Huaxi College of Public Health Sichuan University Chengdu 641000 Sichuan China

Objective To determine whether supplementation of zinc and vitamin A may improve the function of T cells in human PBMC. Methods T cells were separated and cultured in vitro, supplemented with either Zn or vitamin A alone, or both Zn and vitamin A （10^-6 mol/L, 10^-5 mol/L, 10^-4 mol/L）. After harvesting, cell proliferation, cell cycle, apoptosis, expression or function of cell-surface molecules, such as CD^3＋, CD^4＋, and CD^8＋ were detected. Results Higher proliferation level and lower apoptosis level were observed in cells supplemented with both Zn and vitaminA, showing the strongest effect （P〈0.05）. Zn-supplement increased the CD^4＋/CD^3＋ cell percentage, and simultaneously decreased the CD^8＋/CD^3＋ cell population. VA-supplement showed the opposite effect in comparison with Zn-supplement. Conclusion T-cell function can be improved depending on the zinc and/or vitamin A supplemented.

关键词： Vitamin A supplementation Zinc supplementation T cell Immunity

Pterostilbene Ameliorates Glycemic Control,Dyslipidemia and Liver Injury in Type 2 Diabetes Rats

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2020年第5期33卷 365-368页

作者： ZHANG Yu Jing SUN Hua Lei WANG Teng LIU Xin Xin LIU Chang SHEN Fang WANG Bing Ya DING Run Rong LIU Yi Ming HUANG Guo Yu LI Wen Jie LI Xing Department of Nutrition and hygiene College of Public HealthZhengzhou UniversityZhengzhou 450001HenanChina

Type 2 diabetes mellitus(T2DM)is typified by the increment of chronic blood glucose levels that is caused by an absolute and/or a relative deficiency of insulin,accounts for 90%of diabetes and causes a range of compli... 详细信息

关键词： PTE Pterostilbene Ameliorates Glycemic Control,Dyslipidemia and Liver Injury in Type 2 Diabetes Rats

Anticancer effects of oligomeric proanthocyanidins on human colorectal cancer cell line, SNU-C4

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2005年第30期11卷 4674-4678页

作者： Youn-Jung Kim Hae-Jeong Park Seo-Hyun Yoon Mi-Ja Kim Kang-Hyun Leem Joo-Ho Chung Hye-Kyung Kim Department of Dental hygiene Department of Pharmacology Department of Obesity Management College of Oriental Medicine Department of Food and Biotechnology

AIM: Oligomeric proanthocyanidins (OPC), natural polyphenolic compounds found in plants, are known to have antioxidant and anti-cancer effects. We investigated whether the anti-cancer effects of the OPC are induced by apoptosis on human colorectal cancer cell line, ***: Colorectal cancer cell line, SNU-C4 was cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum. The cytotoxic effect of OPC was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenylt-etrazolium bromide (MTT) assay. To find out the apoptotic cell death, 4, 6-diamidino-2-phenylindole (DAPI) staining,terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, reverse transcriptionpolymerase chain reaction (RT-PCR), and caspase-3 enzyme assay were ***: In this study, cytotoxic effect of OPC on SNUC4 cells appeared in a dose-dependent manner. OPC treatment (100 μg/mL) revealed typical morphological apoptotic features. Additionally OPC treatment (100 μg/mL)increased level of BAX and CASPASE-3, and decreased level of BCL-2 mRNA expression. Caspase-3 enzyme activity was also significantly increased by treatment of OPC (100 μg/mL) compared with ***: These data indicate that OPC caused cell death by apoptosis through caspase pathways on human colorectal cancer cell line, SNU-C4.

关键词： Oligomeric pranthocyanidins Apoptosis Anticancer effects Colorectal cancer

Epidemiologic Study of Cancer Mortality among Chloroprene Workers

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 1989年第2期2卷 141-149页

作者： LI SHOUQI DONG QINAN LIU YUQING LIU YINGFEI Research Laboratory of hygiene Toxicology,West China University of Medical Sciences,Chengdu,610044,China Research Laboratory of hygiene Toxicology West China University of Medical Sciences Chengdu.

Both case-control and cohort studies were undertaken from July 1,1969 to June 30,1983 to ascertain whether exposure to chloroprene increases the risk of ***-five cases of cancer deaths were verified,16 of which had histories of exposure to chloroprene ranging from 3 to 23 years(median 11 years)with a latent period of 8-27 years,except for one case of 3 years(median 12.5 years).Fifty-four pairs were obtained by matching the cancer deaths to noncaneer deaths in accordance with strict *** odds ratio for the paired data was 13,x^2=8.64,P <*** average age at death from cancer of workers exposed to chloroprene was 12.7 years younger than that of unexposed workers,t'=2.98,P<*** total cohort consisted of 1213 persons,among whom 149(11.6%)had histories of exposure for over 25 years,381(31.5%) for over 20 years,and 852(70.2%) for over 15 *** SMR for the total cohort was 2.38(P <0.01),and all SMRs for the high-exposure occupations were of significance(P<0.05or P<0.01),in contrast to those of the low-exposure groups whose SMRs were low or ***, a dose-response relationship *** the high-exposure occupations,maintenance me- chanics seem to have the highest risk of cancers,and SMRs for liver,lung,and lymphatic cancers were significant in this *** results suggested that chloroprene exposure increases the risk of developing cancer.1989 Academic Press,Inc.

关键词： work Epidemiologic Study of Cancer Mortality among Chloroprene Workers

Helicobacter pylori vaccination:Is there a path to protection?

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2014年第34期20卷 11939-11949页

作者： Florian Anderl Markus Gerhard Institute for Medical Microbiology Immunology and HygieneTechnische Universit?t MünchenMunich 81675Germany

Helicobacter pylori (H. pylori) is a pathogenic, extracellular bacterium that colonizes the stomach in approximately 50% of the world population. It strongly interacts with the gastric epithelium and mostly causes asymptomatic gastritis. The colonization of H. pylori leads to ulcer development in around 20% of infected patients and may progress to gastric cancer or mucosa-associated lymphoid tissue lymphoma in 1%. Thus, H. pylori is the major cause of gastric cancer worldwide. It has been classified as a class I carcinogen by the World Health Organization. Since its discovery in the early eighties by Warren and Marshall, research has been focused on the investigation of H. pylori biology, host-pathogen interaction, prevention and treatment. Although H. pylori induces a strong humoral and local cellular immune response, the pathogen is not cleared and establishes a chronic infection after encounters in childhood. The ability to colonize the stomach is mediated by several virulence factors that change the host environment, promote adhesion to the epithelium, influence the gastric inflammation and induce immune evasion. H. pylori can be eradicated by antibiotic treatment in combination with a proton-pump inhibitor, but efficacy is decreasing. Current therapies are expensive, have side effects and contribute to increasing antibiotic resistance, underlining the need for novel therapeutics.

关键词： Helicobacter pylori Vaccination Immune response Dendritic cells T-cells