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Cultivar-dependent rhizobacteria community and cadmium accumulation in rice: Effects on cadmium availability in soils and iron-plaque formation

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Journal of Environmental Sciences 2022年第6期34卷 90-102页

作者： Feng Zhang Di Peng Lu Liu Huidan Jiang Lianyang Bai Longping Branch Graduate School of Hunan UniversityChangsha 410125China biotechnology research institute Hunan Academy of Agricultural SciencesChangsha 410125China

The association between the rhizospheric microbial community and Cd accumulation in rice is poorly understood.A field trial was conducted to investigate the different rhizobacterial communities of two rice cultivars with high Cd accumulation(HA)and low Cd accumulation(LA)at four growth stages.Results showed that the Cd content in the roots of the HA cultivar was 1.23-27.53 higher than that of the LA cultivar(0.08-10.5μg/plant)at four stages.The LA cultivar had a significantly lower Cd availability in rhizosphere and a higher quantity of iron plaque(IP)on the root surface than the HA cultivar at four stages.This resulted in the reduction of Cd concentration in IPs and Cd translocation from IP-to-root.Microbial analysis indicated that the LA cultivar formed a distinct rhizobacterial community from the HA cultivar and had lessα-diversity.The rhizosphere of the LA cultivar was enriched in specific bacterial taxa(e.g.,Massilia and Bacillus)involved in Cd immobilization by phosphate precipitation and IP formation by iron oxidization.However,the rhizosphere in the HA cultivar assembled abundant sulfur-oxidizing bacteria(e.g.,Sulfuricurvum)and iron reduction bacteria(Geobacter).They promoted Cd mobilization and reduced IP formation via the metal redox process.This study reveals a potential approach in which specific rhizobacteria decrease or increase Cd accumulation in rice on contaminated soil and provides a new perspective for secure rice production.

关键词： Rice cultivar Cd accumulation Rhizosphere Bacterial community Iron plaque Cd availability

Root Endophyte Shift and Key Genera Discovery in Rice under Barnyardgrass Stress

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Rice science 2023年第2期30卷 160-170,I0040-I0048页

作者： LI Shuyan YAN Qiling WANG Jieyu JIANG Huidan LI Zuren PENG Qiong Long Ping Branch Graduate School of Hunan UniversityChangsha 410125China Hunan Agricultural Biotechnology Research Institute Hunan Academy of Agricultural SciencesChangsha 410125China

Despite increasing knowledge of barnyardgrass(Echinochloa crus-galli) interference with rice, relatively little is known how endophytes improve the ability of rice against barnyardgrass stress. Here, we provided a detailed temporal characterization of rice root-associated microbiomes during co-cultivation with barnyardgrass and a comparison with the microbiomes of weed-free rice plants. Alpha diversity analysis indicated that barnyardgrass had the opposite effects on endophytic bacteria and fungi in rice roots, in terms of the community diversity, richness and coverage at the rice seedling stage. Principal coordinate analysis showed that barnyardgrass had only a minor effect on the community composition of endophytes in rice roots at the rice seedling stage, but showed a significant and maximum interference at the heading stage. Rice recruited many endophytes to resist biotic stress from barnyardgrass, especially for fungi. PICRUSt(phylogenetic investigation of communities by reconstruction of unobserved states) predictive analysis indicated that 23 metabolic pathways of bacteria were overrepresented in rice. In addition, the main trophic mode of fungi was pathotroph according to FUNGuild analysis. A positive correlation between bacteria and fungi in rice roots was found via network analysis. Anaeromyxobacter, Azospira and Pseudolabrys were the vital bacteria, Phaeosphaeria and Funneliformis were the dominant fungi in maintaining the stability of the ecological network. These results provided data and a theoretical basis for the in-depth understanding of what role endophytes play in rice resistance to barnyardgrass stress and will have implications on improving the resistance of rice against biotic stress using root microbiota.

关键词： rice Echinochloa crus-galli biotic stress endophytic bacterium endophytic fungus phylogenetic investigation of communities by reconstruction of unobserved states FUNGuild test

Characterization and mapping of a novel light-dependent lesion mimic mutant lmm6 in rice(Oryza sativa L.)

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Journal of Integrative Agriculture 2015年第9期14卷 1687-1696页

作者： XIAO Gui-qing ZHANG Hai-wen LU Xiang-yang HUANG Rong-feng College of Bioscience and biotechnology Hunan Agricultural University hunan agricultural Bioengineering research institute biotechnology research institute Chinese Academy of Agricultural Sciences

A novel rice lesion mimic mutant (LMM) was isolated from an ethane methyl sulfonate (EMS)-induced 02428 mutant bank. The mutant, tentatively designated as lmm6, develops necrotic lesions in the whole growth period along with changes in several important agronomic traits. We found that the initiation of the lesions was induced by light and cel death occurred in lmm6 accompanied with accumulation of reactive oxygen species (ROS). The lower chlorophyl content, soluble protein content and superoxide dismutase (SOD) activity, the higher malondialdehyde (MDA) content were detected in lmm6 than in the wild type (WT). Moreover, the observation by transmission electronic microscope (TEM) demonstrated that some organel es were damaged and the stroma lamel a of chloroplast was irregular and loose in mesophyl cel of lmm6. In addition, lmm6 was more resistant than WT to rice blast fungus Magnaporthe grisea infection, which was consistent with increased expression of four genes involved in the defense-related reaction. Genetic analysis showed that mutant trait of lmm6 is inherited as a monogenic recessive nuclear gene located on the long arm of chromosome 6. Using simple sequence repeat (SSR) markers, the target gene was ifnal y delimited to an interval of 80.8 kb between markers MM2359 and MM2370, containing 7 annotated genes. Taken together, our results provide the information to identify a new gene involved in rice lesion mimic, which wil be helpful in clarifying the mechanism of cel death and disease resistance in rice.

关键词： rice,lesion mimic mutant,gene mapping,ROS,blast resistance

Synthesis,Crystal Structure and Insecticidal Activity of N-(pyridin-2-ylmethyl)-1-phenyl-1,4,5,6,7,8-hexahydrocyclohepta[c]pyrazole-3-carboxamide

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Chinese Journal of Structural Chemistry 2018年第4期37卷 551-556页

作者：邓希乐周小毛王赞永芮昌辉杨新玲 hunan Agriculture biotechnology research institute Hunan Academy of Agricultural Sciences Department of Applied Chemistry College of ScienceChina Agricultural University Laboratory of Integrated Pest Management in Crops Ministry of AgricultureInstitute of Plant ProtectionChinese Academy of Agricultural Sciences

The title compound N-（pyridin-2-ylmethyl）- 1-phenyl- 1,4,5,6,7,8-hexahydrocy-clohepta[c]pyrazole-3-carboxamide 5 （C21I-I22N40, Mr = 346.42） has been synthesized andstructurally characterized by IR, 1H NMR, 13C NMR, H RMS and single-crystal X-raydiffraction. The crystal crystallizes in monoclinic system, space group P21/n with a = 8.668（2）,b = 22.236（4）, c = 9.539（2） A, β = 108.68（3）°, V = 1786.4（7）/k3, Z = 4, Dx= 1.288 g/cm3,F（000） = 736,μ（MoKa） = 0.649 mm^-1, the final R = 0.0354 and wR = 0.0933 with 3234observed reflections with I 〉 2σ（/）. The benzene and pyrazole rings are nearly coplanar with adihedral angle of 50.977（46）°. The dihedral angle between the central pyrazole and pyridinerings is 11.688（46）°. No classical hydrogen bonds were found in the molecules. Two adjacentmolecules in crystal packing of compound 5 were linked by two intramolecularhydrogen-bonding interactions C（15）-H（15）…O（1） to generate a stable structure. Compound 5had weak insecticidal activity against the diamondback moth （Plutella xylostella）, butexhibited good activity against cotton bollworm （Helicoverpa armigera）.

关键词： synthesis bicyclic pyrazole amide crystal structure insecticidal activity

Comparison of Differentially Expressed Genes Involved in Drought Response between Two Elite Rice Varieties

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Molecular Plant 2012年第6期5卷 1403-1405页

作者： Hai-Wen Zhang Xiao-Wu Pan Yong-Chao Li Li-Yun Wan Xiao-Xiang Li Rong-Feng Huang biotechnology research institute Chinese Academy of Agricultural SciencesBeijing 100081China Rice research institute Hunan Academy of Agricultural SciencesChangsha 410125China

Dear Editor, Drought is the most devastating factor limiting the pro- ductivity and geographical distribution of rice （Oryza sativa）. Several drought-tolerant varieties have been developed from drought-tolerant germplasm. These varieties make it imper- ative to clarity the mechanism of drought responses at the genomic level, which will provide a foundation for future breeding and genetic engineering efforts （Zhou et al., 2007）. Yangdao6 （YD6） was used to breed many elite varieties in China, which was developed from Yangdao2 （YD2） through a series of crosses （Supplemental Figure 1）. Here, we veri- fied that YD6 seedlings showed better drought tolerance than YD2, Yangdao4 （YD4）, and 3021 seedlings （Figure 1A）. After drought treatment for 8 d, over 81.9% of YD2 plants were rolled, which is about three times that of YD6 plants. Recovering normal growth with water, the survival rates of YD6 and YD2 seedlings were 92.2% and 65.6%, respectively.

关键词：繁殖基因稻属遗传工程胰岛素幼苗植物灾害

Citron C-05 inhibits both the penetration and colonization of Xanthomonas citri subsp.citri to achieve resistance to citrus canker disease

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Horticulture research 2020年第1期7卷 1981-1992页

作者： Hongyan Fu Mingming Zhao Jing Xu Limei Tan Jian Han Dazhi Li Meijun Wang Shunyuan Xiao Xianfeng Ma Ziniu Deng College of Horticulture Hunan Agricultural University410128 ChangshaHunanChina National Center for Citrus Improvement 410128 ChangshaHunanChina hunan Horticultural research institute 410125 ChangshaHunanChina institute for Bioscience and biotechnology research&Department of Plant Sciences and Landscape Architecture University of Maryland College ParkRockvilleMD 20850USA

Citrus canker,caused by Xanthomonas citri subsp.citri(Xcc),is a serious bacterial disease that affects citrus production worldwide.Citron C-05(Citrus medica)is the only germplasm in the Citrus genus that has been identified to exhibit strong resistance to Xcc.However,it has not been determined when,where,and how Xcc is restricted in the tissues of Citron C-05 during the infection process.In the present study,we investigated the spatiotemporal growth dynamics of an eGFP-labeled virulent Xcc(eGFP-Xcc)strain in Citron C-05 along with five susceptible biotypes(i.e.,lemon,pummelo,sour orange,sweet orange,and ponkan mandarin)upon inoculation via the spraying or leaf infiltration of a bacterial suspension.The results from extensive confocal laser scanning microscopy analyses showed that while Xcc grew rapidly in plants of all five susceptible genotypes,Xcc was severely restricted in the epidermal and mesophyll cell layers of the leaves of Citron C-05 in the early stage of infection.Not surprisingly,resistance against Xcc in Citron C-05 was found to be associated with the production of reactive oxygen species and hypersensitive response-like cell death,as well as greater upregulation of several defense-related genes,including a pathogenesis-related gene(PR1)and a glutathione S-transferase gene(GST1),compared with sweet orange as a susceptible control.Taken together,our results not only provide further valuable details of the spatiotemporal dynamics of the host entry,propagation,and spread of Xcc in both resistant and susceptible citrus plants but also suggest that resistance to Xcc in Citron C-05 may be attributed to the activation of multiple defense mechanisms.

关键词： citrus Xanthomonas resistance

Phase separation of GRP7 facilitated by FERONIAmediated phosphorylation inhibits mRNA translation to modulate plant temperature resilience

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Molecular Plant 2024年第3期17卷 460-477页

作者： Fan Xu Long Wang Yingbin Li Junfeng Shi Dorothee Staiger Feng Yu state Key Laboratory of Chemo/Biosensing and Chemometrics College of Biologyand Hunan Key Laboratory of Plant Functional Genomics and Developmental RegulationHunanUniversityChangsha 410082P.R.China State Key Laboratory of Hybrid Rice Hunan Agricultural Biotechnology Research InstituteHunan Academy of Agricultural SciencesChangsha 410125P.R.China RNA Biology and Molecular Physiology Faculty of BiologyBielefeld University33615 BielefeldGermany

Changes in ambient temperature profoundly affect plant growth and performance.Therefore,the molecu-larbasis of plant acclimation to temperature fluctuation is of great interest.In this study,we discovered that GLYCINE-RICH RNA-BINDING PROTEIN 7(GRP7)contributes to cold and heat tolerance in Arabidopsis thaliana.We found that exposure to a warm temperature rapidly induces GRP7 condensates in planta,which can be reversed by transfer to a lower temperature.Cell biology and biochemical assays revealed that GRP7 undergoes liquid-liquid phase separation(LLPS)in vivo and in vitro.LLPS of GRP7 in the cyto-plasm contributes to the formation of stress granules that recruit RNA,along with the translation machinery component eukaryotic initiation factor 4E1(elF4E1)and the mRNA chaperones COLD SHOCK PROTEIN 1(CSP1)and CSP3,to inhibit translation.Moreover,natural variations in GRP7 affecting the residue phos-phorylated by the receptorkinase FERONIA alter its capacity to undergo LLPS and correlate with the adap-tation of some Arabidopsis accessions to a widertemperature range.Taken together,ourfindings illustrate the role of translational control mediated by GRP7 LLPS to confer plants with temperature resilience.

关键词： fluctuating temperature glycine-rich RNA-binding protein phase separation cold shock proteins translation

4-Phenylbutyric acid accelerates rehabilitation of barrier function in IPEC-J2 cell monolayer model

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Animal Nutrition 2021年第4期7卷 1061-1069页

作者： Qian Jiang Jie Yin Jiashun Chen Xiaokang Ma Miaomiao Wu Xilong Li Kang Yao Bi'e Tan Yulong Yin Animal Nutritional Genome and Germplasm Innovation research Center College of Animal Science and TechnologyHunan Agricultural UniversityChangshaHunan 410128China Key Laboratory of Feed biotechnology of Ministry of Agriculture and Rural Affairs Feed Research InstituteChinese Academy of Agricultural SciencesBeijing 100081China Laboratory of Animal Nutritional Physiology and Metabolic Process Institute of Subtropical AgricultureChinese Academy of SciencesChangshaHunan 410125China

As the first line of defence against pathogens and endotoxins crossing the intestine-blood barrier,the intestinal epithelial barrier plays a determinant role in pigs’health and growth.4-Phenylbutyric acid(4-PBA),an aromatic fatty acid,was reported to benefit homeostasis of endoplasmic reticulum and protein synthesis.However,whether 4-PBA affects intestinal epithelial barrier function in pigs is unknown.This study aimed to explore the effects of 4-PBA on the intestinal barrier function,using in vitro models of well-differentiated intestinal porcine epithelial cell(IPEC-J2)monolayers in the transwell plates.Cell monolayers with or without 4-PBA(1.0 mmol/L)treatment were challenged with physical scratch,deoxynivalenol(DON,2.0μg/mL,48 h),and lipopolysaccharide(LPS,5.0μg/mL,48 h),respectively.Transepithelial electrical resistance(TEER)and fluorescein isothiocyanate-dextran(FD-4)permeability were measured to indicate barrier integrity and permeability.Real-time PCR and Western blot were conducted to determine relative gene and protein expressions of tight junction proteins.As expected,physical scratch,DON,and LPS challenges decreased TEER and increased FD-4 permeability.4-PBA treatment accelerated cell mitigation and rehabilitation of the physical scratch-damaged intestinal epithelial barrier but did not alleviate DON or LPS induced barrier damage.However,once 48-h DON and LPS challenges were removed,rehabilitation of the epithelial barrier function of IPEC-J2 monolayer was accelerated by the 4-PBA treatment.Also,the relative gene and protein expressions of zonula occludens-1(ZO-1),occludin,and claudin-1 were further upregulated by the 4-PBA treatment during the barrier rehabilitation.Taken together,4-PBA accelerated the IPEC-J2 cell monolayer barrier recovering from physical scratch,DON-,and LPS-induced damage,via enhancing cell mitigation and expressions of tight junction proteins.

关键词： 4-Phenylbutyric acid Intestinal barrier Tight junction Intestinal porcine epithelial cell Deoxynivalenol Lipopolysaccharide

Co-planted barnyardgrass reduces rice yield by inhibiting plant above-and belowground-growth during post-heading stages

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The Crop Journal 2021年第5期9卷 1198-1207页

作者： Zichang Zhang Jingjing Cao Tao Gu Xia Yang Qiong Peng Lianyang Bai Yongfeng Li institute of Plant Protection Jiangsu Academy of Agricultural SciencesKey Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding BaseNanjing 210014JiangsuChina biotechnology research Center Hunan Academy of Agricultural SciencesChangsha 410125HunanChina

Barnyardgrass (Echinochloa spp.) is the most common noxious weed in rice paddies as it inhibits rice growth and reduces grain yield.To date,little information is available on above-and belowgroundgrowth changes in rice due to neighboring barnyardgrass.This study aimed to investigate the changes in root traits and shoot growth of rice when it is grown with different kinds of barnyardgrass.Japonica rice plants (var.Nanjing 9108) were co-cultured with two varieties of Echinochloa crusgalli (L.) Beauv.(EP,var.mitis (pursh) Petern;EH,var.zelayensis (H.B.K.) Hitchc),and E.colonum (L.) Link (EL) in the field in 2017 and 2018.Four treatments included control (i.e.,weed free rice plants) and co-cultures with each of three barnyardgrasses (EP,EH,and EL).The results revealed that EP,EH,and EL treatments significantly reduced rice grain yields by 30.6%–36.2%,42.5%–46.5%,and 10.6%–14.3%,respectively.Shoot growth including shoot dry weight,leaf photosynthetic rate,zeatin (Z) and zeatin riboside (ZR) in grains,and activities of key enzymes involved in sucrose-to-starch conversion in grains and root traits,such as length density,root dry weight,total absorbing surface area,active absorption surface area,oxidation activity,and Z+ZR contents in roots were dramatically reduced during post-heading stages of rice when grown with the three kinds of barnyardgrass.Moreover,above-mentioned rice shoot growth indices were strongly and positively correlated with root traits.These results suggested the decrease in rice shoot growth and root traits during post-heading stages contributes to the reduction in the rice yield when it grows with barnyardgrass neighbors.

关键词： Rice Barnyardgrass Grain yield Root traits Shoot traits