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Influence of in vitro capacitation time on structural and functional human sperm parameters

Asian Journal of Andrology 2020年第5期22卷 447-453页

作者： Paula Saez-Espinosa Natalia Huerta-Retamal Laura Robles-Gomez Manuel Aviles Jon Aizpurua Irene Velasco Alejandro Romero Maria Jose Gomez-Torres Department of Biotechnology Faculty of ScienceUniversity of AlicanteAlicante 03080Spain FISABI0-University Hospital of San Juan de Alicante Service of Gynecology and ObstetricsSan Juan de Alicante 03550Spain Departm ent of Cell Biology and Histology Faculty of MedicineUniversity of Murcia and IMIB-ArrixacaMurcia 30100Spain IVF Spain Reproductive MedicineAlicante 03540Spain University Hospital of San Juan de Alicante Service of Gynecology and ObstetricsSan Juan de Alicante 03550Spain human fertility cathedra University of AlicanteAlicante 03080Spain.

A cascade of dramatic physiological events is linked to the sperm acrosome reaction and binding to the oocyte's zona pellucida during human sperm capacitation.However,structural and functional sperm changes during capacitation currently remain poorly defined.Here,we performed a multibiomarker approach based on the utilization of sperm concentration,motility,viability,morphology,acrosome reaction,tyrosine phosphorylation,DNA fragmentation,and lectin-binding sites to analyze the impact caused by swim-up selection times(uncapacitated,1 h capacitated,and 4 h capacitated)on sperm function and structure in normozoospermic samples.We found that a 4 h swim-up capacitation increased sperm quality,because a large number of cells with normal morphology and lower DNA fragmentation rates were recovered.Furthermore,the long-term capacitation induced a higher percentage of cells with tyrosine phosphorylation of the principal piece as well as a redistribution of lectin-binding sites.Overall,the multivariate biomarkers analyzed showed a less variable distribution on spermatozoa recovered after 4 h capacitation than that with the shorter capacitation time.These findings stress the importance of capacitation time as a relevant factor in sperm quality with potential biological reproductive implications both for basic research and in assisted reproduction techniques.

关键词： acrosome reaction capacitation DNA fragmentation glycocalyx lectin-binding sites membrane integrity protein phosphorylation sperm selection

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