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Construction of an anchoring SSR marker genetic linkage map and detection of a sex-linked region in two dioecious populations of red bayberry

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Horticulture Research 2020年第1期7卷 2262-2270页

作者： Yan Wang Hui-Min Jia Yu-Tong Shen Hai-Bo Zhao Qin-Song Yang Chang-Qing Zhu De-Li Sun Guo-Yun Wang Chao-Chao Zhou Yun Jiao Chun-Yan Chai Li-Ju Yan Xiong-Wei Li Hui-Juan Jia Zhong-Shan Gao Fruit Science Institute College of Agriculture and BiotechnologyZhejiang University310058 HangzhouChina Shanghai center for Plant Stress Biology CAS Center for Excellence in Molecular Plant SciencesChinese Academy of Sciences201602 ShanghaiChina Yuyao Forestry technology extension center 315400 NingboChina Institute of Forestry Ningbo Academy of Agricultural ScienceNingboChina Cixi Forestry technology extension center 315300 CixiChina Linhai Forestry technology extension center 317000 TaizhouChina Forest&Fruit Tree Institute Shanghai Academy of Agricultural Sciences201403 ShanghaiChina

Red bayberry(Morella rubra)is an evergreen fruit tree found in southern China whose whole-genome sequence has recently been *** updated the linkage map of the species by adding 118 SSR markers and the femalespecific marker MrFT2_*** integrated map included eight linkage groups and spanned 491 *** sexassociated markers were identified,six of which were located in linkage group 8,in agreement with the previously reported location of the sex-determining *** MrFT2_BD-SEX marker was genotyped in 203 cultivated *** the females of the accessions,we found two female-specific alleles,designated W-b(151 bp)and W-d(129 bp).We previously found that‘Dongkui’,a female cultivar,could produce viable pollen(we refer to such plants‘Dongkui-male’)and serve as the paternal parent in *** genotypes of the MrFT2_BD-SEX marker were W-b/Z in‘Biqi’and W-d/Z in‘Dongkui-male’.The progeny of a cross between these parents produced a 3:1 female(W-)to male(ZZ)ratio and the expected 1:1:1:1 ratio of W-b/W-d:W-b/Z:W-d/Z:Z/*** addition,the flowering and fruiting phenotypes of all the F1 progeny fit their *** results confirm the existence of ZW sex determination and show that the female phenotype is controlled by a single dominant locus(W)in a small genomic region(59 kb and less than 3.3 cM).Furthermore,we have produced a homozygous“super female”(WW)that should produce all-female offspring in the F2 generation,providing a foundation for commercial use and presenting great potential for use in modern breeding programs.

关键词： breeding cultivar linkage

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Assessment of wheat variety distinctness using SSR markers

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Journal of Integrative Agriculture 2015年第10期14卷 1923-1935页

作者： WANG Li-xin QIU Jun CHANG Li-fang LIU Li-hua LI Hong-bo PANG Bin-shuang ZHAO Chang-ping Beijing Engineering and Technique Research center for Hybrid Wheat Beijing Academy of Agricultural and Forestry Sciences National Agriculture technology extension and Service center Ministry of Agriculture National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science Chinese Academy of Agricultural Sciences

Assessment of variety distinctness is important for both the registration and the protection of particular variety. However, the current testing system, which assesses a range of morphological characters of each pair of varieties grown side-by-side, is time-consuming and is not suitable for the assessment of hundreds of samples. The objective of this study was to develop a procedure for the assessment of wheat variety distinctness using simple sequence repeat（SSR） markers. A comparison between the molecular and morphological profile of 797 varieties was made. On the basis of the comparison, pairs of varieties with a genetic similarity value（GSV） ≤90% were deemed to be distinct, accounting for ~85% of varieties assessed in wheat regional trials. For the remaining ~15% of varieties, GSVs between different varieties were 〉90%, among which ~35% were not distinct and the other ~65% were distinct. Therefore, if given a GSV〉90%, the pairs of varieties should be morphologically assessed in the field. To avoid any errors in the assessments, we proposed the elimination of contaminant plants from the sample before comparing the varietal genotypes, scoring of the genotype at each locus with a pair of allele numbers when constructing a molecular profile, and faithfully recording two alleles at a non-homozygous locus. To reduce the workload and cost, a three-grade markers comparison among varieties is suggested. In addition, 80 SSR markers and a technical procedure for assessment of wheat variety distinctness have been proposed. Based on the procedure, the distinctness assessment of ~85% of all wheat varieties is completed in our laboratory annually. Consequently, total field assessment has been reduced considerably.

关键词： wheat variety distinctness assessment SSR markers

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Improved root zone temperature buffer capacity enhancing sweet pepper yield via soil-ridged substrate-embedded cultivation in solar greenhouse

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International Journal of agricultural and Biological Engineering 2018年第2期11卷 41-47页

作者： Guohai Fu Zonggeng Li Wenke Liu Qichang Yang Institute of Environment and Sustainable Development in Agriculture Chinese Academy of Agricultural Sciences/Key Laboratory of Energy Conservation and Waste Management of Agricultural StructuresMinistry of AgricultureBeijing 100081China National agricultural technology extension and Service center Beijing 100125China

A novel soilless cultivation method,called as soil-ridged substrate-embedded cultivation(SSC)was invented,and an experiment was designed to investigate root zone temperature and production efficiency of sweet pepper cultivated by two SSC patterns,i.e.,SSC-P(polyethylene groove inserted)and SSC-W(wire-mesh groove inserted),and also other two cultivation methods,i.e.,soil ridge(SR)and naked polyethylene groove(PG).Results showed that PG,SSC-P and SSC-W increased the average minimal root zone temperature by 1.01℃,0.75℃,and 1.16℃ compared to SR(16.33℃)during March 16-20,*** June 1-5,SSC-P and SSC-W decreased the average maximal root zone temperature by 1.28℃ and 1.29℃ compared to SR(34.99℃),while PG increased it by 1.44℃.PG,SSC-P,and SSC-W decreased the differences of average daytime and night time temperatures by 1.34℃,2.13℃,and 2.88℃ compared to SR(4.56℃)during early ***,SSC-P and SSC-W decreased temperature differences of average daytime and night time by 0.9℃ and 1.07℃ compared to SR(0.95℃)during later stage,but PG improved by 2.85℃.Temperature difference of daytime and night time of SSC-W was minimal,and the temperature difference between the diurnal highest and the lowest temperature of SSC-W was also *** buffer capacity of SSC-W was slightly better than that of ***-W significantly improved the growth of sweet pepper compared to ***,fruit yield per square meter of sweet pepper cultivated on SSC-P and SSC-W improved by 21.24%and 50.33%,respectively compared to SR(3.06 kg/m^(2)),while PG lowered the yield by 13.72%.SSC-W was a better SSC pattern compared with SSC-P in terms of production efficiency.

关键词： soil cultivation SSC substrate cultivation solar greenhouse diurnal temperature difference root zone temperature

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Application Effect of Integrated Water and Fertilizer technology for Tomato

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Asian agricultural Research 2017年第11期9卷 62-65页

作者： Haimin KONG Ruohui LU Zhejiang agricultural technology extension center

Integrated water and fertilizer technology has the advantages of saving water,fertilizer and labour,which is widely applied in tomato plantation. Integrated water and fertilizer technology in topdressing and whole process of big and small tomatoes were studied,and their application effects were contrasted and analyzed,and application advantages and scopes of the two models were concluded.

关键词： Tomato Integrated water and fertilizer technology in whole process Integrated water and fertilizer technology in topdressing

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Comparison of biochar properties from 5 kinds of halophyte produced by slow pyrolysis at 500℃

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Biochar 2022年第1期4卷 1003-1012页

作者： Hongyang Xiao Qimei Lin Guitong Li Xiaorong Zhao Jianzhong Li Erzhen Li Department of Soil and Water Science College of Land Science and TechnologyChina Agricultural UniversityBeijing 100193China agricultural and Animal Husbandry technology extension and Service center of Wuyuan County Bayannur 015100Inner MongoliaChina

Glycophyte biomass-derived biochars are currently concerned in most ***,little attention is given to the char-acteristics of halophyte-derived *** this study,five typical halophytes of euhalophytes(Suaeda altissima,Suaeda salsa,and Kalidium foliatum),recretohalophytes(Phragmites australis),and pseudohalophytes(Tamarix chinensis)which are widely distributed in the arid and semiarid regions of northwestern China were selected for producing biochars with a slow pyrolysis process at 500℃for 1 *** harvested biochars were characterized in elemental content,pores,surface area,and surface charges,and then their potential value as a soil conditioner was *** results showed that the halophyte-derived biochars had variable ash and Na+contents,ranging from 7.26 to 23.64%and 1.06 to 33.93 g kg^(−1)*** EC value of the biochars ranged from 1.76 to 23.45 mS cm^(−1).The biochar derived from Suaeda altissima had a very low specific surface area(SSA),3.50 m^(2)g^(−1),while that derived from Phragmites australis(BPA)had a very high SSA,344.02 m^(2)g^(−1).All the biochars carried both positive and negative *** foliatum biochar(BKF)possessed more nega-tive charges,while Suaeda altissima biochar(BSA)contained more positive *** general,the halophyte biochars had a higher ash content and lower point of zero net charge(PZNC)value,compared with the biochars derived from glycophytes,which would imply their higher potential value as an acidic soil conditioner.

关键词： Halophyte Biochar Structural elements Mineral elements Surface charges

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Interactions between vegetation dynamic and edaphic factors in the Great Salt Desert of central Iran

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Journal of Arid Land 2021年第2期13卷 123-134页

作者： Hossein BASHARI Seyed Mehrdad KAZEMI Soghra POODINEH Mohammad R MOSADDEGHI Mostafa TARKESH Seyed Mehdi ADNANI Department of Natural Resources Isfahan University of TechnologyIsfahan ***Iran Department of Soil Science College of AgricultureIsfahan University of TechnologyIsfahan ***Iran Forests and Rangelands Research Department Qom Agricultural and Natural Resources Research and Education CenterAgricultural ResearchEducation and Extension Organization(AREEO)Qom ***Iran

Investigating the relationships between vegetation dynamic and edaphic factors provide management insights into factors affecting the growth and establishment of plant species and vegetation communities in saline *** aim of this study was to assess the spatial variability of various vegetation communities in relation to edaphic factors in the Great Salt Desert,central *** vegetation communities were identified using the physiognomy-floristic *** and density of vegetation communities were determined using the transect plot *** soil samples were collected from major horizons of fifteen profiles in vegetation communities,and analyzed in terms of following soil physical and chemical characteristics:soil texture,soluble Na+concentration,sodium adsorption ratio(SAR),electrical conductivity(EC),pH,organic matter content,soluble Mg2+and Ca2+concentrations,carbonate and gypsum contents,and spontaneously-and mechanically-dispersible clay *** analysis was used to investigate the relationships between vegetation dynamic and edaphic *** generalized linear method(GLM)was used to find the plant species response curves against edaphic *** showed that plant species responded differently to edaphic factors,in which soluble sodium concentration,EC,SAR,gypsum content and soil texture were identified as the most discriminative edaphic *** studied plant species were also found to have different ecological requirements and tolerance to edaphic factors,in which Tamarix aphylla and Halocnemum strobilaceum were identified as the most salt-resistant species in the ***,the presence of Artemisia sieberi was highly related to soil sand and gypsum *** results implied that exploring the plant species response curves against edaphic factors can assist managers to lay out more appropriate restoration plans in similar arid areas.

关键词： desert generalized linear method mechanically-dispersible clay ordination plant species response curves spontaneously-dispersible clay

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First report of Leptosphaeria sclerotioides on Brassica napus in Northwestern China

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Oil Crop Science 2023年第2期8卷 73-80页

作者： Mingxue Shang Xing Lyv Jing Zhang Mingde Wu Long Yang Qingxia Guan Guoqing Li State Key Laboratory of agricultural Microbiology and Key Laboratory of Plant Pathology of Hubei Province Huazhong Agricultural UniversityWuhan430070China Longxi agricultural technology extension center Longxi CountyGansu ProvinceLongxi748199China

In 2020,diseased seedlings of winter oilseed rape(Brassica napus)with cankered taproots as well as abundant sclerotium-like structures in the soil surrounding the roots were found in Longxi County of Gansu province of northwestern China.A fungus with production of pycnidia was isolated from the diseased roots,and it was identified based on morphological characteristics,molecular phylogeny(ITS,LSU)and PCR detection with the specific *** fungus was identified as Leptosphaeria sclerotioides Gruyter,Aveskamp&Verkley[anamorph:Phoma sclerotioides(Preuss)ex Sacc.].Re-inoculation of isolates of P9 and P10 of *** on winter oilseed rape(*** cultivar‘Zhongshuang No.9’)in Wuhan caused formation of abundant sclerotium-like structures in soil surrounding the roots,but failed to produce root cankers as those observed in Gansu possibly due to lack of long peroid of low-temperature conditioning in *** spite of this,plant height,pod number and seed yield of oilseed rape were significantly reduced in the treatment with *** P9 and P10,compared to the control *** the best of our knowledge,this is the first report of *** on *** in China and the finding broadened our understanding about the natural distribution of this psychrophilic fungus.

关键词： Leptosphaeria sclerotioides Brassica napus China

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Performances of an air thermal energy utilization system developed with fan-coil units in large-scale plastic tunnels covered with external blanket

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International Journal of agricultural and Biological Engineering 2022年第5期15卷 56-62页

作者： Chengji Zong Zibin Xiao Weitang Song Pingzhi Wang Guifang Zhang Ming Li College of Water Resources and Civil Engineering China Agricultural UniversityBeijing 100083China Yinchuan Xingqing District agricultural technology extension center Yinchuan750001China Key Laboratory of agricultural Engineering in Structure and Environment Ministry of Agriculture and Rural AffairsBeijing 10008

To improve the problem of low temperature at night in winter due to the lack of thermal storage in large-span plastic tunnels,an air thermal energy utilization system(ATEUS)was developed with fan-coil units to heat a large-scale plastic tunnel covered with an external blanket(LPTEB)on winter *** ATEUS was composed of nine fan-coil units mounted on top of the LPTEB,a water reservoir,pipes,and a water circulation *** the heat exchange between the air and the water flowing through the coils,the thermal energy from the air can be collected in the daytime,or the thermal energy in the water can be released into the LPTEB at *** sunny days,the collected thermal energy from the air in the daytime(E_(c))and released thermal energy at night(E_(r))were 0.25-0.44 MJ/m^(2) and 0.24-0.38 MJ/m^(2),*** ATEUS as a heating system,its coefficient of performance(COP),which is the ratio of the heat consumption of LPTEB to the power consumption of ATEUS,ranged from 1.6-2.1.A dynamic model was also developed to simulate the water temperature(T_(w)).Based on the simulation,E_(c) and E_(r) on sunny days can be increased by 60%-73%and 38%-62%,respectively,by diminishing the heat loss of the water reservoir and increasing the indoor air temperature in the period of collecting thermal ***,the COP can reach 2.6-3.8,and the developed ATEUS can be applied to heating the LPTEB in a way that conserves energy.

关键词： large scale plastic tunnel air thermal energy utilization system energy conservation COP

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Assessment of the contribution percentage of inherent soil productivity of cultivated land in China

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Journal of Integrative Agriculture 2019年第11期18卷 2619-2627页

作者： WANG Shi-chao WANG Jin-zhou ZHAO Ya-wen REN Yi XU Ming-gang ZHANG Shu-xiang LU Chang-ai National Engineering Laboratory of Farmland Cultivation technology/Institute of agricultural Resources and Regional Planning Chinese Academy of Agricultural SciencesBeijing 100081P.R.China The National Agro-Tech extension and Service center Beijing 100125P.R.China

The contribution percentage of inherent soil productivity(CPISP)refers to the ratio of crop yields under no-fertilization versus under conventional fertilization with the same field *** is a comprehensive measure of soil *** study used 1086 on-farm trials(from 1984-2013)and 27 long-term field experiments(from 1979-2013)to quantify changes in ***,we present CPISP3 values,which reflect the CPISP states during the first three years after site establishment,for a series of sites at different locations in China collected in 1984-1990(the 1980s),1996-2000(the 1990s),and 2004-2013(the 2000s).The results showed that the average CPISP3 value for three crops(wheat,rice,and maize)was 53.8%.Historically,the CPISP3 in the 1990s(57.5%)was much higher than those in the 1980s(50.3%),and the 2000s(52.0%)(P≤0.05).Long-term no-fertilization caused CPISP levels to gradually decline and then stabilize;for example,in a mono-cropping system with irrigation,the CPISP values in Northwest and Northeast China declined by 4.5 and 4.0%,respectively,each year for the first ten years,but subsequently,the CPISP values *** contrast,the CPISP for upland crops in double-cropping systems continued to decrease at a rate of 1.1%per *** CPISP for upland-paddy cropping decreased very slowly(0.07%per year),whereas the CPISP for paddy cropping decreased sharply(3.1%per year,on average)for the first two years and then remained steady during the following ***,upland crops in double-cropping systems consume the most inherent soil productivity,whereas paddy fields are favourable for maintaining a high level of ***,our results demonstrate a need to further improve China’s CPISP3 values to meet growing productivity demands.

关键词： crop yield inherent soil productivity typical cropping system planting years cropspecies

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Effects of seed mixture sowing with transgenic Bt rice and its parental line on the population dynamics of target stemborers and leafrollers, and non-target planthoppers

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Insect Science 2019年第4期26卷 777-794页

作者： Zhuo Li Li-Kun Li Bin Liu Long Wang Megha N. Parajulee Fa-Jun Chen Department of Entomology Nanjing Agricultural University Nanjing China Texas A&M University Agri Life Research and extension center Lubbock Texas USA

The widespread planting of insect-resistant crops has caused a dramatic shift in agricultural landscapes, thus raising concerns about the potential impacts on both target and non-target pests. In this study, we examined the potential effects of intra-specific seed mixture sowing with transgenic Bt rice (Bt) and its parental non-transgenic line (Nt)(100% Bt rice [Bt100], 5% Nt+95% Bt [Nt05Bt95], 10% Nt+90% Bt [Nt|0Bt90], 20% Nt+80% Bt [Nt2oBtso], 40% Nt+60% Bt [Nt4oBt6O] and 100% Nt rice [Ntjoo]) on target and non-target pests in a 2-year field trial in southern China. The occurrence of target pests, Sesamia inf evens. Chilo suppressalis and Cnaphalocrocis medinalis, decreased with the increased ratio of Bt rice, and the mixture ratios with more than 90% Bt rice (Btjoo and Nt()5Bt95) significantly increased the pest suppression efficiency, with the lowest occurrences of non-target planthoppers, Nilaparvata lugens and Sogatella jurcijera in Ntioo and NtosBtgs. Furthermore, there were no significant differences in 1000?grain dry weight and grain dry weight per 100 plants between Btjoo and NtosBtgs,Seed mixture sowing of Bt rice with <10%(especially 5%) of its parent line was sufficient to overcome potential compliance issues that exist with the use of block or structured refuge to provide most effective control of both target and non-target pests without compromising the grain yield. It is also expected that the strategy of seed mixture sowing with transgenic Bt rice and the nontransgenic parental line would provide rice yield stability while decreasing the insecticide use frequency in rice production.

关键词： cultivar diversity occurrence and damage seed mixture sowing target and non-target pests transgenic Bt rice yield

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