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Science Bulletin 2025年第2期70卷 176-179页

作者： Liang Chen Lifang Xiao Shilong Guo Nan Yang Conrad C.Labandeira Chungkun Shih Chaofan Shi Dong Ren College of Life Sciences Capital Normal UniversityBeijing 100048China Guangdong key laboratory of Animal conservation and Resource utilization Guangdong Public Laboratory of Wild Animal Conservation and UtilizationInstitute of ZoologyGuangdong Academy of SciencesGuangzhou 510260China Department of Paleobiology National Museum of Natural HistorySmithsonian InstitutionWashingtonDC 20013USA Department of Entomology University of MarylandCollege ParkMD 20742USA School of Earth Sciences and Engineering Guangdong Provincial Key Lab of Geological Processes and Mineral ResourcesSun Yat-sen UniversityGuangzhou 510275China

Palaeoecological research is essential to understanding the interaction between organisms and their environment during the evolutionary ***,the study of fossil insect communities is challenging because of a limited fossil record and the unavailability of data,which makes it difficult to assess the associations of insects with their environment,especially the role of local climate.

关键词： Jurassic Middle process.

Gas-Solid Flow,Combustion Characteristics,and Gas Emissions in a 75 t/h CFB Boiler Based on the CPFD Method

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Journal of Thermal Science 2025年第1期34卷 323-336页

作者： HUANG Xiankun BAI Zhang ZHU Xiaoli WANG Shuoshuo MU Lin GONG Liang College of New Energy China University of Petroleum(East China)Qingdao 266580China key laboratory of Ocean Energy utilization and Energy conservation of Ministry of Education School of Energy and Power EngineeringDalian University of TechnologyDalian 116024China

A 3D simulation using Computational Particle Fluid Dynamics(CPFD)methods was used to calculate coal combustion in a 75 t/h industrial-scale circulating fluidized bed(CFB)*** characteristics,gas-solid flow characteristics,and gaseous pollutant emissions of CFB boilers from combustion ignition to stable operation were systematically evaluated in this *** show that the temperature distribution is relatively uniform throughout the *** the combustion process unfolds within the boiler,the gas composition curve strikingly portrays the inverse correlation between CO_(2)and O_(2)*** the combustion reaction progresses,it becomes evident that the concentration of CO_(2)progressively increases,while the concentration of O_(2)concurrently *** inverse relationship underscores the fundamental combustion reaction,where carbon-based fuels react with oxygen to produce carbon dioxide and release ***,a comprehensive analysis has revealed that,from ignition to stable combustion,both nitric oxide(NO)and sulfur dioxide(SO_(2))emissions exhibit a declining *** reduction in pollutant generation is attributed to the improvement in combustion *** complete combustion leads to lower levels of unburned hydrocarbons,which are prone to NO ***,the sulfur content in the fuel is more efficiently oxidized to sulfur trioxide(SO_(3))or bound in sulfates,reducing SO_(2)*** steady state in the simulation,the SO_(2)mass flow rate varies significantly with the furnace height,gradually increasing from 0.07 kg·s^(-1)at 4 m at the bottom of the furnace to a peak of 0.078 kg·s^(-1)at 8 m in the center,and then decreasing to 0.06 kg·s^(-1)at 20m at the top of the furnace.

关键词： circulating fluidized bed particle flow combustion CPFD modeling

The fourth exon confers antagonistic activity of OsMFT1 and OsMFT2 in rice pre-harvest sprouting

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The Crop Journal 2025年第1期13卷 135-144页

作者： Jialing Zhang Fei Liu Yang Kuang Ming Luo Chengcai Chu Fan Xu College of Agronomy and Biotechnology Southwest UniversityChongqing 400715China Guangdong laboratory for Lingnan Modern Agriculture State Key Laboratory for Conservation and Utilization of Subtropical Agro-BioresourcesCollege of AgricultureSouth China Agricultural UniversityGuangzhou 510642GuangdongChina

Pre-harvest sprouting(PHS)describes the germination of physiologically mature grains in spikes prior to harvest in cereal *** could seriously decrease grain yield and quality,which makes it a major constraint to cereal production worldwide.A number of PHS-associated genes in cereals have been reported;however,the molecular mechanisms underlying PHS remain largely ***,we report a CRISPRCas9 mutant with severe PHS in a paddy *** mutated gene OsMFT2 encodes a phosphatidylethanolamine-binding protein(PEBP).Intriguingly,the OsMFT1,in the same PEBP family,had the opposite effect in controlling rice PHS as does *** tests of seeds of chimeric protein-expressing plants revealed that the fourth exon conferred the antagonistic activity of OsMFT1 and OsMFT2 in rice ***,two lines of these plants showed elevated grain numbers per panicle,implying that chimeric protein has potential to significantly increase ***,transcriptome analysis and genetic studies indicated that OsMFT1 and OsMFT2 performed opposing functions in rice PHS owing to three co-regulated genes that being contrastingly affected by OsMFT1 and ***,it seemed that the proper combination of PEBP family members could obtain optimal PHS resistance and high yield.

关键词： Rice Pre-harvest sprouting PEBP MFT

Small auxin-up RNA gene OsSAUR33 promotes seed aging tolerance in rice

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Journal of Integrative Agriculture 2025年第1期24卷 61-71页

作者： Shan Sun Wenjun Li Yanfen Fang Qianqian Huang Zhibo Huang Chengjing Wang Jia Zhao Yongqi He Zhoufei Wang laboratory of Seed Science and Technology South China Agricultural University/Guangdong Key Laboratory of Plant Molecular Breeding/State Key Laboratory for Conservation and Utilization of Subtropical Agro-BioresourcesGuangzhou 510642China School of Agriculture Yunnan UniversityKunming 650091China

Seed aging tolerance during storage is generally an important trait for crop production, yet the role of small auxin-up RNA genes in conferring seed aging tolerance is largely unknown in rice. In this study, one small auxin-up RNA gene, OsSAUR33, was found to be involved in the regulation of seed aging tolerance in rice. The expression of OsSAUR33 was significantly induced in aged seeds compared with unaged seeds during the seed germination phase. Accordingly, the disruption of OsSAUR33 significantly reduced seed vigor compared to the wild type(WT) in response to natural storage or artificial aging treatments. The rice OsSAUR33 gene promotes the vigor of aged seeds by enhancing their reactive oxygen species(ROS) level during seed germination, and the accumulation of ROS was significantly delayed in the aged seeds of Ossaur33 mutants in comparison with WT during seed germination. Hydrogen peroxide(H_(2)O_(2)) treatments promoted the vigor of aged seeds in various rice varieties. Our results provide timely theoretical and technical insights for the trait improvement of seed aging tolerance in rice.

关键词： auxin-responsive gene reactive oxygen species rice seed aging seed vigor

Development of an Agrobacterium tumefaciens-mediated transformation system for somatic embryos and transcriptome analysis of LcMYB1's inhibitory effect on somatic embryogenesis in Litchi chinensis

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Journal of Integrative Agriculture 2025年第2期24卷 594-609页

作者： Yaqi Qin Bo Zhang Xueliang Luo Shiqian Wang Jiaxin Fu Zhike Zhang Yonghua Qin Jietang Zhao Guibing Hu State key laboratory for conservation and utilization of Subtropical Agro-bioresources/key laboratory of Biology and Genetic Improvement of Horticultural Crops (South China) Ministry of Agriculture and Rural Affairs/Guangdong Litchi Engineering Research CenterCollege of HorticultureSouth China Agricultural University

Litchi has great economic significance as a global fruit crop. However, the advancement of litchi functional genomics has encountered substantial obstacles due to its recalcitrance to stable transformation. Here, we present an efficacious Agrobacterium tumefaciens-mediated transformation system in somatic embryos of ‘Heiye' litchi. This system was developed through the optimization of key variables encompassing explant selection, A. tumefaciens strain delineation, bacterium concentration, infection duration, and infection methodology. The subsequent validation of the transformation technique in litchi was realized through the ectopic expression of LcMYB1, resulting in the generation of transgenic calli. However, the differentiation of transgenic calli into somatic embryos encountered substantial challenges. To delineate the intricate molecular underpinnings of LcMYB1's inhibitory role in somatic embryo induction, a comprehensive transcriptome analysis was conducted that encompassed embryogenic calli(C), globular embryos(G), and transgenic calli(TC). A total of 1,166 common differentially expressed genes(DEGs) were identified between C-vs.-G and C-vs.-TC. Gene Ontology(GO) annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway analysis revealed that these common DEGs were mostly related to plant hormone signal transduction pathways. Furthermore, RT-qPCR corroborated the pronounced down-regulation of numerous genes that are associated with somatic embryo induction within the transgenic calli. The development of this transformation system provides valuable support for functional genomics research in litchi.

关键词： Litchi chinensis A. tumefaciens transformation LcMYB1 somatic embryo

Enhancing the photocatalytic efficiency by the molecular modification effect derived from pollutant adsorption on highly crystalline BiOBr

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Journal of Environmental Sciences 2025年第2期148卷 263-273页

作者： Yang Jin Tongyin Liu Yanpeng Mao Fan Li Chun Hu National Engineering laboratory for Reducing Emissions from Coal Combustion Engineering Research Center of Environmental Thermal Technology of Ministry of EducationShandong Key Laboratory of Energy Carbon Reduction and Resource UtilizationSchool of Energy and Power EngineeringShandong UniversityJinan 250061China Beijing Enterprises Water GroupInvestment Limited Beijing 100102China Institute of Environmental Research at Greater Bay Key Laboratory for Water Quality and Conservation of the Pearl River DeltaMinistry of EducationGuangzhou UniversityGuangzhou 510006China key laboratory of Drinking Water Science and Technology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China

The adsorption of pollutants can not only promote the direct surface reaction,but also modify the catalyst itself to improve its photoelectric characteristics,which is rarely studied for water treatment with inorganic photocatalyst.A highly crystalline BiOBr(c-BiOBr)was synthesized by a two-step preparation *** to the calcination,the highly crystalline enhanced the interface interaction between pollutant and *** complex of organic pollutant and[Bi_(2)O_(2)]^(2+)could promote the active electron transfer from the adsorbed pollutant to c-BiOBr for the direct pollutant degradation by holes(h^(+)).Moreover,the pollutant adsorption actually modified c-BiOBr and promoted more unpaired electrons,which would coupling with the photoexcitation to promote generate more O_(2)^(•-).The molecular modification effect derived from pollutant adsorption significantly improved the removal of *** work strongly deepens the understanding of the molecularmodification effect from the pollutant adsorption and develops a novel and efficient approach for water treatment.

关键词： Highly crystalline BiOBr Pollutant adsorption Molecular modification Photocatalysis Water treatment

A revised classification of Dryopteridaceae based on plastome phylogenomics and morphological evidence,with the description of a new genus,Pseudarachniodes

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Plant Diversity 2025年第1期47卷 34-52页

作者： Zheng-Yu Zuo Germinal Rouhan Shi-Yong Dong Hong-Mei Liu Xin-Yu Du Li-Bing Zhang Jin-Mei Lu Germplasm Bank of wild Species and Yunnan Key Laboratory of Crop Wild Relatives OmicsKunming Institute of BotanyChinese Academy of SciencesKunming 650201YunnanChina Institut de Systematique EvolutionBiodiversite(ISYEB)Museum National d’Histoire NaturelleCNRSSorbonne UniversiteEcole Pratique des Hautes EtudesUniversite des AntillesCP3957 rue Cuvier 75005ParisFrance key laboratory of Plant Resources conservation and Sustainable utilization South China Botanical GardenChinese Academy of SciencesGuangzhou 510650GuangdongChina Center for Integrative conservation&Yunnan key laboratory for conservation of Tropical Rainforests and Asian Elephants Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMenglun 666303YunnanChina Missouri Botanical Garden 4344 Shaw BlvdSt Louis 63110MOUSA Chengdu Institute of Biology Chinese Academy of SciencesChengdu 610041SichuanChina State key laboratory of Plant Diversity and Specialty Crops Kunming Institute of BotanyChinese Academy of SciencesKunming 650201YunnanChina

Dryopteridaceae are the largest fern family and include nearly 20%of extant fern diversity,with 24 currently recognized *** and delineation of genera within this family have varied *** three-subfamily classification of Dryopteridaceae was based primarily on molecular phylogenetic relationships but lacked morphological evidence,and the phylogenetic relationships of the subfamilies and genera of Dryopteridaceae are only partially resolved.A comprehensive and robust phylogeny is urgently *** heterogeneous morphology of the current members of Dryopteridaceae makes the family and its subfamilies difficult to define by single morphological characteristics or even character *** carried out phylogenetic analyses to reconstruct a highly supported phylogeny of *** analyses recovered 24 strongly supported clades grouped into seven major clades of *** morphological characters including habit,rhizome shape,frond morphology,rachis-costae architecture,appendages on stipe base and lamina,and soral arrangement were found to be informative for identifying different major clades and clades in *** on phylogenetic reconstruction and morphological analysis,we presented an updated infra-familial classification of Dryopteridaceae with seven subfamilies and 24 genera including four newly proposed subfamilies(Ctenitidoideae,Lastreopsidoideae,Pleocnemioideae,and Polystichopsidoideae).Morphological character combinations of each subfamily are summarized,and a key is *** genera sensu PPG I are recognized,with Stigmatopetris reclassified into Dryopteridoideae and Arthrobotrya considered a synonym of Teratophyllum.A new genus Pseudarachniodes is *** revised classification will serve as a foundational framework for future investigations on taxonomy,biogeography,and diversification of the most species-rich Dryopteridaceae in ferns.

关键词： Ctenitidoideae Infra-familial Morphological diagnostic characters Phylogenomics Rachis-costae architecture Stigmatopetris

Exploring economic viable species via assessing a tropic-specific microalgal collection

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Journal of Oceanology and Limnology 2025年第1期43卷 210-218页

作者： Chen MA Ni WANG Yi XIN Qinhua GAN Wenxu ZHOU Yandu LU State key laboratory of Marine Resource utilization in South China Sea School of Marine Biology and FisheriesHainan UniversityHaikou 570228China Hainan Engineering&Research Center of Marine Bioactives and Bioproducts Haikou 570228China Haikou Innovation Platform for Research&utilization of Algal Bioresource Hainan UniversityHaikou 570228China Hainan Provincial key laboratory of Tropical Hydrobiotechnology Hainan UniversityHaikou 570228China

Locations around the equator provide unique habitats for microalgae presumably with exceptional *** develop microalga-derived product systems,we previously created a(sub)tropical microalgal *** this study,two Chlorella strains(MEM176 and MEM193),adaptive to typical tropical climate,were isolated from the *** production performance was evaluated where both strains showed a robust growth in high temperatures and light ***,the strains MEM176 and MEM193 produced 503.6-mg/(L·d)and 411.3-mg/(L·d)biomass,respectively,with high contents of *** commercial and nutritional values were emphasized by amino acid compositions(e.g.,proline,valine,and phenylalanine).Particularly,higher amounts of proline were revealed in MEM176(47.9 mg/g dry biomass(DW))and MEM193(47.9 and 59.2 mg/g DW)than available commercial *** with MEM193,MEM176 produced 129.7%more lipids in which unsaturated fatty acids(particularly linoleic acid andα-linolenic acid)account for 65%of the total ***,it is promising to explore the potential of these Chlorella strains as food additives via outdoor cultivation in tropical area,notably MEM176 that exhibits superiority as sources of essential amino acids and valuable fatty acids.

关键词： Chlorella tropical species food additive microalga

Organic fertilizer enhances soil aggregate stability by altering greenhouse soil content of iron oxide and organic carbon

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Journal of Integrative Agriculture 2025年第1期24卷 306-321页

作者： Lijun Ren Han Yang Jin Li Nan Zhang Yanyu Han Hongtao Zou Yulong Zhang College of Land and Environment Shenyang Agricultural University/National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources/Key Laboratory of Arable Land Conservation in Northeast ChinaMinistry of Agriculture and Rural AffairsShenyang 110866China

Both soil organic carbon (SOC) and iron (Fe) oxide content, among other factors, drive the formation and stability of soil ***, the mechanism of these drivers in greenhouse soil fertilized with organic fertilizer is not well *** a 3-year field experiment, we aimed to investigate the factors which drive the stability of soil aggregates in greenhouse *** explore the impact of organic fertilizer on soil aggregates, we established four treatments:no fertilization (CK);inorganic fertilizer (CF);organic fertilizer (OF);and combined application of inorganic and organic fertilizers(COF).The application of organic fertilizer significantly enhanced the stability of aggregates, that is it enhanced the mean weight diameter, geometric mean diameter and aggregate content (%) of>0.25 mm aggregate *** and COF treatments increased the concentration of SOC, especially the aliphatic-C, aromatic-C and polysaccharide-C components of SOC, particularly in>0.25 mm *** fertilizer application significantly increased the content of free Fe(Fed), reactive Fe (Feo), and non-crystalline Fe in both bulk soil and ***, non-crystalline Fe showed a positive correlation with SOC content in both bulk soil and *** non-crystalline Fe and SOC were significantly positively correlated with>2 mm mean weight ***, we believe that the increase of SOC, aromatic-C, and non-crystal ine Fe concentrations in soil after the application of organic fertilizer is the reason for improving soil aggregate stability.

关键词： organic fertilizer soil aggregates soil organic carbon iron oxides greenhouse soil

Dual-Mode Colorimetric and Fluorescent Detection of Tumor Cells Based on Gold Nanoparticles-Loaded Phosphine Covalent Organic Frameworks

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Journal of Analysis and Testing 2025年第01期 21-31页

作者： Bilian Li Min Zhou Chun Zhao Lin Xiao Tianle Qi Hanbin Xu Linjiang Guo Guobao Ning Xiaokang Lu Keming Zhu Hui Zhao Can-Peng Li School of Chemical Science and Technology Yunnan University Affiliated Hospital of Yunnan University School of Life Science Yunnan University laboratory for conservation and utilization of Bioresource Yunnan University

Circulating tumor cells(CTCs) are important markers for cancer. The part of tumor cells that are detached from the primary tumor or metastatic tumor and enter the blood circulation is called CTCs. It is crucial to develop a rapid, accurate, and easy-to-implement diagnostic system for CTCs for early tumor diagnosis and for monitoring progression of the disease. In this work, we reported a colorimetric and fluorescent dual-mode assay for the detection of CTCs. Our assay used magnetic nanoparticles and aptamer for CTCs capture and gold nanoparticles-loaded covalent organic frameworks(Au@COFs) for signal amplification, respectively. The magnetic nanoparticles were modified with folic acid to capture CTCs by interaction between folic acid and the folate receptor overexpressed on the surface of tumor cells. The covalent organic frameworks were engineered to have both nitro-reductase-like and glucose–oxidase-like activities. The nitro-reductase-like activity converted the substrate p-nitrophenol to p-nitroaniline for colorimetric detection, and the glucose-oxidase-like activity enabled fluorescence detection. Specifically, Au@COFs catalyzed glucose oxidation and generated hydrogen peroxide to oxidize Fe 2+to Fe 3+, which converted MIL(Al)-MOF to MIL(Fe)-MOF through ion exchange, resulting in the fluorescence quenching of MIL(Al)-MOF. Our assay showed high sensitivity with a detection limit of 17 cells/mL using MCF-7 cells as model cancer cells. This work provided an e fficient and ultrasensitive strategy for CTCs detection and has potential applications in cancer identification and diagnosis. Dual-mode detection system, combining colorimetric and fluorescent signals, integration of magnetic nanoparticles and aptamers for CTC capture, and utilization of covalent organic frameworks loaded with gold nanoparticles for signal amplification can improve sensitivity and accuracy.

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