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Odorant-binding protein 19 in Monochamus alternatus involved in the recognition of a volatile strongly emitted from ovipositing host pines

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insect Science 2024年第1期31卷 134-146页

作者： Ruinan Yang Dongzhen Li Shancheng Yi Yi Wei Manqun Wang hubei insect resources utilization and sustainable pest management key laboratory College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina key laboratory of Forest Protection of National Forestry and Grassland Administration Ecology and Nature Conservation InstituteChinese Academy of ForestryBeijingChina

Monochamus alternatus is the primary carrier of pine wood nematodes,which pose a serious threat to Pinus spp.in many countries.Newly emerging M.alternatus adults feed on heathy host pines,while matured adults transfer to stressed host pines for mating and oviposition.Several odorant-binding proteins(OBPs)of M.alternatus have been proved to aid in the complex process of host location.To clarify the corresponding relations between OBPs and pine volatiles,more OBPs need to be studied.In this research,MaltOBP19 showed a specific expression in the antennae and mouthparts of M.alternatus,and it was marked in 4 types of antenna sensilla by immunolocalization.Fluorescence binding assays demonstrated the high binding affinity of MaltOBP19 with camphene and myrcene in vitro.In Y-tube olfactory experiments,M.alternatus adults were attracted by camphene and RNAi of OBP19 via microinjection significantly decreased their attraction index.Myrcene induced phobotaxis,but RNAi had no significant effect on this behavior.Further,we found that ingesting dsOBP19 produced by a bacteria-expressed system with a newly constructed vector could lead to the knockdown of MaltOBP19.These results suggest that MaltOBP19 may play a role in the process of host conversion via the recognition of camphene,which has been identified to be strongly released in stressed host pines.In addition,it is proved that knockdown of OBP can be achieved by oral administration of bacteria-expressed double-stranded RNA in M.alternatus adults,providing a new perspective in the control of M.alternatus.

关键词： bacteria-expressed dsRNA camphene Japanese pine sawyer beetle MaltOBP19 RNAi stressed host pines

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miRNAs targeting CYP6ER1 and CarE1 are involved in nitenpyram resistance in Nilaparvata lugens

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insect Science 2022年第1期29卷 177-187页

作者： Kaikai Mao Ruoheng Jin Zhijie Ren Junjie Zhang Zhao Li Shun He Kangsheng Ma Hu Wan Jianhong Li hubei insect resources utilization and sustainable pest management key laboratory College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina

The evolution of nitenpyram resistance has been confirmed to be related to overexpression of two key metabolic enzyme genes, CYP6ER1 and CarE1, in Nilaparvata lugens, a highly destructive rice pest that causes substantial economic losses and has developed insecticide resistance. As microRNAs (miRNAs) are important post-transcriptional regulators of gene expression, whether they are involved in nitenpyram resistance is poorly understood in N. lugens. In this study, knockdown of key genes in the miRNA biogenesis pathway (Dicer1, Drosha, and Argonaute1) changed CYP6ER1 and CarE1 abundance, which confirmed the importance of miRNAs in nitenpyram resistance. Furthermore, global screening of miRNAs associated with nitenpyram resistance in N. lugens was performed, and a total of 42 known and 178 novel miRNAs were identified;of these, 57 were differentially expressed between the susceptible and resistant strains, and two (novel_85 and novel_191) were predicted to target CYP6ER1 and CarE1, respectively. Luciferase reporter assays demonstrated that novel_85 and novel_191 bind to the CYP6ER1 and CarE1 coding regions, respectively, and downregulate their expression. Moreover, modulating novel_85 and novel_191 expression by injection of miRNA inhibitors and mimics significantly altered N. lugens nitenpyram susceptibility. This is the first study to systematically screen and identify miRNAs associated with N. lugens nitenpyram resistance, and provides important information that can be used to develop new miRNA-based targets in insecticide resistance management.

关键词： CarE1 CYP6ER1 miRNAs Nilaparvata lugens nitenpyram resistance

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Juvenile hormone regulates photoperiod-mediated male reproductive diapause via the methoprene-tolerant gene in the ladybeetle Harmonia axyridis

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insect Science 2022年第1期29卷 139-150页

作者： Qiao Gao Bei Li Bing-Xin Wei Wen Liu Pan Wang Jia-Lu Wang Xing-Miao Zhou Xiao-Ping Wang hubei key laboratory of insect resources utilization and sustainable pest management College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan430070China Department of Plant Protection Wuhan Vegetable Research InstituteWuhan430065China

Juvenile hormone (JH) absence induces photoperiod-mediated reproductive diapause, which is characterized by reproductive cessation. Although the role of methoprene-tolerant (Met)-mediated JH signaling in photoperiod-mediated female reproduction has been well documented, its role in male reproduction remains unclear. In this study, we investigated the role of JH in regulating photoperiod-mediated development of the male internal reproductive system (IRS) in the predatory ladybeetle Harmonia axyridis (Pallas). In a previous study, we found that adult male H. axyridis reared under either a short-day (SD) or long-day (LD) photoperiod had obvious differences in IRS development, but we were unable to identify the regulators of male reproductive diapause. In this study, we found that beetles reared under an SD photoperiod had significantly lower JH titer and a relatively undeveloped male IRS compared with those reared under an LD photoperiod. Additionally, application of the JH analog (JHA) methoprene promoted IRS development. Furthermore, Met knockdown strongly blocked JH signaling in males reared under the LD photoperiod, thereby slowing IRS development. Moreover, exogenous JHA did not reverse the suppressed development of the male IRS caused by Met knockdown. These results indicate that photoperiod regulates male IRS development in H. axyridis through a conserved Met-dependent JH signaling pathway.

关键词： Harmonia axyridis juvenile hormone male reproductive diapause methoprene-tolerant photoperiod RNAi

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A sublethal concentration of afidopyropen suppresses the population growth of the cotton aphid, Aphis gossypii Glover(Hemiptera: Aphididae)

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Journal of Integrative Agriculture 2022年第7期21卷 2055-2064页

作者： MA Kang-sheng TANG Qiu-ling LIANG Ping-zhuo LI Jian-hong GAO Xi-wu Department of Entomology China Agricultural UniversityBeijing 100193P.R.China hubei insect resources utilization and sustainable pest management key laboratory College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan 430070P.R.China

The cotton aphid,Aphis gossypii Glover(Hemiptera:Aphididae),is an important insect pest of cotton crops worldwide.The objectives of this study were to determine the acute toxicity of afidopyropen and whether there are any effects of sublethal concentrations of afidopyropen on the biological characteristics of A.gossypii.The results showed that afidopyropen possesses high acute toxicity to adult A.gossypii with a 72 h LC_(50) value of 1.062 mg L^(–1).A sublethal concentration(LC_(10))of afidopyropen significantly decreased adult longevity,fecundity and oviposition days of female adults in both F0 and F1 generations.The total pre-adult survival of F_(1) progeny was also significantly reduced by 30%at the LC_(10) of afidopyropen.In addition,the nymph developmental time,pre-adult period,adult pre-reproductive period(APRP),and total pre-reproductive period(TPRP)of the F1 progeny were significantly prolonged compared with the control.Several population parameters,including the net reproductive rate(R_(0)),intrinsic rate of increase(r)and finite rate of increase(λ)of F_(1) progeny were significantly decreased by a sublethal afidopyropen concentration exposure.These results indicated that sublethal concentration of afidopyropen can significantly suppress A.gossypii population growth.It would be useful for assessing the overall effects of afidopyropen on A.gossypii.

关键词： afidopyropen Aphis gossypii sublethal effects longevity fecundity

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IDH knockdown alters foraging behavior in the termite Odontotermes formosanus in different social contexts

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Current Zoology 2021年第6期67卷 609-620页

作者：徐焕黄求应高勇勇吴佳 Ali HASSAN 刘昱彤 hubei insect resources utilization and sustainable pest management key laboratory Huazhong Agricultural UniversityWuhanHubei 430070China

Foraging,as an energy-consuming behavior,is very important for colony survival in termites.How energy metabolism related to glucose decomposition and adenosine triphosphate(ATP)production influences foraging behavior in termites is still unclear.Here,we analyzed the change in energy metabolism in the whole organism and brain after silencing the key metabolic gene isocitrate dehydrogenase(IDH)and then investigated its impact on foraging behavior in the subterranean termite Odontotermes formosanus in different social contexts.The IDH gene exhibited higher expression in the abdomen and head of O.formosanus.The knockdown of IDH resulted in metabolic disorders in the whole organism.The dsIDH-injected workers showed significantly reduced walking activity but increased foraging success.Interestingly,IDH knockdown altered brain energy metabolism,resulting in a decline in ATP levels and an increase in IDH activity.Additionally,the social context affected brain energy metabolism and,thus,altered foraging behavior in O.formosanus.We found that the presence of predator ants increased the negative influence on the foraging behavior of dsIDH-injected workers,including a decrease in foraging success.However,an increase in the number of nestmate soldiers could provide social buffering to relieve the adverse effect of predator ants on worker foraging behavior.Our orthogonal experiments further verified that the role of the IDH gene as an inherent factor was dominant in manipulating termite foraging behavior compared with external social contexts,suggesting that energy metabolism,especially brain energy metabolism,plays a crucial role in regulating termite foraging behavior.

关键词： energy supply foraging success isocitrate dehydrogenase Odontotermes formosanus social contexts walking activity

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An InR/mir 9a/NIUbx regulatory cascade regulates wing diphenism in brown planthoppers

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insect Science 2021年第5期28卷 1300-1313页

作者： Xiang Li Mu-Hua Zhao Miao-Miao Tian Jing Zhao Wan-Lun Cai Hong-Xia Hua hubei insect resources utilization and sustainable pest management key laboratory College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina

Wing polymorphism significantly contributes to the ecological success of some insect species.For example,the brown planthopper(BPH)Nilaparvata lugens,which is one of the most destructive rice pests in Asia,can develop into either highly mobile long-winged or highly fecund short-winged adult morphs.A recent study reported a highly provocative result that the Hox gene UIltrabithorax(Ubx)is expressed in BPH forewings and showed that this wing development gene is differentially expressed in nymphs that develop into long-winged versus short-winged morphs.Here,we found that Ubx may be a mir-9a target,and used dual luciferase reporter assays and injected micro RNA(miRNA)mimics and inhibitors to confirm the interactions between mir-9a and NIUbx.We measured the mir-9a and NIUbx expression profiles in nymphs and found that the expression of these two biomolecules was negatively correlated.By rearing BPH nymphs on host rice plants with different nutritional status,we were able to characterize a regulatory cascade between insulin receptor genes,mir-9a,and NIUbx that regulate wing length in BPHs.When host quality was low,NIInRI expression in the nymph terga increased and NIInR2 expression decreased;this led to a higher mir-9a level,which in turn reduced the NIUbx transcript level and ultimately resulted in longer wing lengths.Beyond extending our understanding of the interplay between host plant status and genetic events that modulate polymorphism,we demonstrated both the upstream signal and miRNA-based regulatory mechanism that control Ubx expression in BPH forewings.

关键词： mir-9a Nilaparvata lugens UIltrabithorax wing polymorphism

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Wolbachia enhances expression of NICYP4CE1 in Nilaparvata lugens in response to imidacloprid stress

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insect Science 2021年第2期28卷 355-362页

作者： Tingwei Cai Yunhua Zhang Yu Liu Xiaoqian Deng Shun He Jianhong Li Hu Wan hubei insect resources utilization and sustainable pest management key laboratory College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina

The brown planthopper,Nilaparvata lugens,is one of the main insect pests of rice.The N.lugens gene NICYP4CE1 encodes cytochrome P450 monooxygenase(P450),which is a key enzyme in the metabolism of the insecticide imidacloprid.Previous research has suggested that the expression of NICYP4CEI is induced by imidacloprid stress,but the effect of bacterial symbionts on its expression has not been determined.The results of this study show that exposure to subtoxic imidacloprid changed the structure of the bacterial symbiont community in N.lugens.Specifically,the total bacterial content increased but the bacterial species diversity significantly decreased.Wolbachia accounted for the largest proportion of bacteria in N.lugens;its abundance significantly increased after subtoxic imidacloprid exposure.The transcript level of NICYP4CEI was significantly increased by imidacloprid,but this effect was significantly weakened after Wolbachia was cleared with tetracycline.This result suggests that Wolbachia enhances the expression of N1CYP4CE1 to promote the detoxification metabolic response to imidacloprid stress.Understanding the effect of bacterial symbionts on gene expression in the host provides a new perspective on interactions between insecticides and their target insect pests,and highlights that subtoxic imidacloprid exposure may raise the risk of insecticide resistance by altering the structure of bacterial symbiont communities.

关键词： host-microbe interaction imidacloprid NICYP4CE1 P450 Wolbachia

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Advances in the use of the RNA interference technique in Hemiptera

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insect Science 2013年第1期20卷 31-39页

作者： Jie Li Xiao-Ping Wang Man-Qun Wang Wei-Hua Ma Hong-Xia Hua hubei insect resources utilization and sustainable pest management key laboratory Colleage of Plant Science and Technology Huazhong Agricultural University Wuhan China

RNA interference （RNAi） suppresses the expression of target genes by post- transcriptional regulation. Because double-stranded RNA （dsRNA） mediated gene silenc- ing is a conserved mechanism in many eukaryotes, RNAi has become a valuable tool for unveiling gene function in many model insects. Recent research has also shown that RNAi can also be effective in the downregulation of target genes in Hemiptera. In this review, we discuss the use of the RNAi technique in gene functional analysis in hemipterans, highlighting the methods of dsRNA uptake by these insects and discuss the knock-down efficiency of these techniques. Although the RNAi technique has disadvantages, our pri- mary goal here is to determine whether it can be exploited further in the discovery of new ~ene functions, and as a oest control strategy, in some important HemiDteran oests.

关键词： dsRNA gene function Hemiptera pest control RNA interference

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A trade-off between antipredatory behavior and pairing competition produced by male-male tandem running in three Reticulitermes species

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insect Science 2015年第4期22卷 560-568页

作者： Gang-Hua Li Long Liu Chao-Liang Lei Qiu-Ying Huang hubei insect Resource utilization and sustainable pest management key laboratory Huazhong Agricultural University Wuhan 430070 China

Due to the omnipresent risk of predation, termites have evolved many antipreda- tory behaviors. The two related species Reticulitermes speratus and R. chinensis have been demonstrated to use homosexual tandem running to decrease individual predation risk after shedding their wings. In this study, we tested risk of predation in the termite R. flaviceps, which is distantly related to the above two species. We determined that homosexual tandem running also led to low individual predation risk in dealates of R. flaviceps. Moreover, by combining a predation model with a competition model, we observed a typical trade-off phenomenon between antipredatory behavior and pairing competition produced by male- male tandem running in the above three Reticulitermes species. Our results indicated that male-male tandem running could effectively protect disadvantaged individuals from being caught, but disadvantaged individuals would be easily eliminated in pairing competition after male-male tandem running, suggesting that male-male tandem running can pro- mote population evolution in termites by repeatedly removing the relatively inferior male individuals.

关键词： antipredatory behavior pairing competition tandem running termites trade-off

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insecticidal activities of garlic substances against adults of grain moth, Sitotroga cerealella （Lepidoptera： Gelechiidae）

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insect Science 2012年第2期19卷 205-212页

作者： Feng-Lian Yang Fen Zhu Chao-Liang Lei hubei insect resources utilization and sustainable pest management key laboratory Huazhong Agricultural UniversityWuhanChina

The angoumois grain moth, Sitotroga cerealella （Olivier）, is one of the most serious stored grain pests around the world. In attempts to reduce the losses caused by the moth and to suppress its populations, the fumigant activities, behavioral influence and ovipositional inhibition of garlic （Allium sativum） essential oil and its two major components, diallyl disulfide and diallyl trisulfide, were investigated against the adult grain moth. Their effects on reduction in survival of first instar larvae to adult emergence were also evaluated. Results showed that these three materials （garlic essential oil, diallyl disulfide and diallyl trisulfide） had significant fumigant activity with 50% lethal concentration values at 1.33, 0.99, and 1.02/zL/L air space, respectively; meanwhile, the three materials possessed high behavioral deterrent activities against adults in the Y-tube olfactometer. When applied to rice grains, these materials reduced adult longevity and inhibited oviposition, with ovipositional inhibition above 70% at a concentration of 1.5uL/25 g in either no-choice or two-choice tests. In short, the study showed that both diallyl disulfide and diallyl trisulfide, like garlic essential oil, acted as fitrnigants, produced behavioral deter- rence and inhibited oviposition against angoumois grain moth. Our work here indicates that diallyl disulfide and diallyl trisulfide may serve as potential alternatives for grain protectants since both of them can be prepared easily from readily available chemicals.

关键词： behavioral deterrence essential oil fumigant garlic oviposition inhibition,Sitotroga cerealella

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