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Identification and Characterization of Peptides Mimicking the Epitopes of Metalloprotease of Schistosoma Japonicum

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Cellular & Molecular Immunology 2005年第3期2卷 219-223页

作者： Lianfei Tang Yuxiao Chen Linqian Wang Shunke Zhang Xianfang Zeng Xinyuan Yi Department of Parasitology Xiangya School of Medicine Central SouthUniversity Changsha 410008 China Technology Centre Hunan Entry-Exit Inspection and Quarantine BureauChangsha China

In an attempt to isolate and characterize peptides mimicking epitopes of metalloprotease and explore their immunological protection against Schistosoma japonicum （S. japonicum）, polyclonal anti-metalloprotease sera was prepared to screen a 12-mer random peptide library to isolate phages binding specially to antisera IgG. Then, phage ELISA, animal immunization, DNA sequencing, Western blotting and enzymatic activity neutralizing analysis were used to characterize the selected phage clones. All of ten randomly picked clones were shown to be positive. Five peptides of different amino acid sequences deduced from DNA sequences were obtained and two of them （peptides 2 and 3） could induce significant reduction （31.0% and 31.8%, respectively） in worm burden and high reduction （52.6% and 54.9%, respectively） in liver eggs per gram （LEPG）, while, unexpectedly, others （peptides 1, 4 and 5） could not elicit enough protection against infection of S. japonicum. Peptides 2 and 3 could be recognized by S. japonicum infected mouse sera （IMS） and could elicit neutralizing Abs. The results show that peptides 2 and 3 are antigenic and immunogenic. They are true mimics of epitopes of metalloprotease and useful as novel vaccine candidates against ***. Cellular ＆ Molecular Immunology. 2005;2（3）：219-223.

关键词： phage displayed peptide library epitope Schistosomajaponicum metalloprotease

Determination of Potassium Ferrocyanide in Foods by Resonance Rayleigh Scattering Method with Basic Triaminotriphenylmethane Dyes

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Chinese Chemical Letters 2006年第12期17卷 1595-1598页

作者： Yong Li LI Zhong Fang LIU Li Sha XIE Ling KONG Shao Pu LIU School of Chemistry and Chemical Engineering Southwest University Chongqing 400715 Sichuan entry-exit inspection and quarantine Chengdu 610041

In this work, a sensitive, rapid and simple method for the determination of trace amounts of potassium ferrocyanide in salinized foods and table salt using EV as a RRS probe is established. The detection limit （3σ） of the EV system is 7.8 ng/mL. This new method is more suitable for the determination of the trace amounts of potassium ferrocyanide in colour salinized foods and it can not be disturbed by the color of salinized foods.

关键词： Resonance Rayleigh scattering basic triaminotdphenylmethane dyes potassiumferrocyanide.

Highly sensitive detection of NT-proBNP by molecular motor

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Genes & Diseases 2017年第1期4卷 37-40页

作者： Jie Zhang Yao-Gen Shu Beijing entry-exit inspection and quarantine bureau Beijing100026China Institute of Theoretical Physics Chinese Academy of SciencesBeijing100190China

FoF1-ATPase is an active rotary motor,and generates three-ATP for each *** saturated substrate concentration,the motor can achieve about 103 r.p.m,which means one motor can generate about 105 ATP molecules during 30 ***,we constituted a novel nanodevice with a molecular rotary motor and a“battery”,FoF1-ATPase and chromatophore,and presented a novel method of sandwich type rotary biosensor based on εsubunit with one target-to-one motor,in which one target corresponds 105 ATP molecules as detection signals during 30 *** target such as NT-proBNP detection demonstrated that this novel nanodevice has potential to be developed into an ultrasensitive biosensor to detect low expressed targets.

关键词： Active nanodevice FoF1-ATPase Highly sensitive detection Molecular motor NT-proBNP

One New Method of Nucleic Acid Amplification-Loop-mediated Isothermal Amplification of DNA

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Virologica Sinica 2008年第3期23卷 167-172页

作者： Xue-en FANG Jian LI Qin CHEN School of Life Science Shanghai University Shanghai 200444 China Shanghai entry-exit inspection and quarantine bureau Shanghai 200135 China

Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method, which amplifies DNA with high specificity, sensitivity, rapidity and efficiency under isothermal conditions using a set of four specially designed primers and a Bst DNA polymerase with strand displacement activity. The basic principle, characteristics, development of LAMP and its applications are summarized in this article.

关键词： Nucleic acid amplification Loop-mediated isothermal amplification (LAMP) Application

Rapid and sensitive detection of pesticides by surface-enhanced Raman spectroscopy technique based on glycidyl methacrylate-ethylene dimethacrylate(GMA-EDMA) porous material

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Chinese Chemical Letters 2013年第4期24卷 332-334页

作者： Qing-Qing Li Yi-Ping Du Ying Xu Xuan Wang Shi-Qian Ma Jin-Pei Geng Peng Cao Tao Sui Shanghai Key Laboratory of Functional Materials Chemistry & Research Centre of Analysis and Test East China University of Science and Technology Yantai entry-exit inspection and quarantine bureau

A novel method of fast and sensitive SERS detection using GMA-EDMA porous material combined with a miniature device was reported in this study. A 100 μL solution containing sample, silver colloid and NaC1 was evenly mixed to ensure the sample molecules would adsorb onto silver nanoparticles. Then the mixture was added onto the porous material surface slowly, so that the aggregation of silver colloid would stay on the surface while the liquid components would flow away. This technology can improve the sensitivity of SERS detection. By this method, two pesticides tricyclazole and paraquat were successfully detected at concentrations of 5 × 10 3 mg/L and I × 10-3 mg/L, respectively.

关键词： rSurface-enhanced Raman spectroscopyGMA-EDMA porous materialTricyclazoleParaquat

Diarylheptanoids from the root bark of Juglans cathayensis

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Chinese Chemical Letters 2013年第6期24卷 521-523页

作者： Juan Li Jia-Xiang Sun Heng-Yi Yu Zu-Yu Chen Xiao-Ya Zhao Han-Li Ruan Department of Pharmacy Tongji Hospital Affiliated Tongji Medical CollegeHuazhong University of Science and Technology School of Pharmacy Tongji Medical College of Huazhong University of Science and Technology hubei entry-exit inspection and quarantine bureau

A new diarylheptanoid glucoside,（8R,9R）-17-methoxy-2-oxatricyclo[13.***.2.1^（3.7）]icosa-l（17）,3（20）,4,6, 15,18-hexaene-4,9,10-triol-9-O-β-D-glucopyranoside（1）,namely jugcathayenoside,together with two known diarylheptanoids,（＋）-galeon（2） and 4-hydroxy-17-methoxy-2-oxatricyclo[13.***.2.1^（3,7） ]icosa-1（17）,3（20）,4,6,15,18-hexaene-9-one（3）,were isolated from the root bark of Juglans cathayensis. Their structures were elucidated on the basis of extensive spectroscopic data analysis.

关键词： Juglans cathayensis Diarylheptanoid glucoside Jugcathayenoside

Air Velocity Fluctuation in Reed Groove of Air-Jet Loom

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Journal of Donghua University(English Edition) 2011年第2期28卷 170-176页

作者：魏孟媛薛文良陆维民陈革祝章琛 Key Laboratory of Textile Science & Technology Ministry of Education Donghua University Shanghai entry-exit inspection and quarantine bureau College of Mechanical Engineering Donghua University

The airflow in the reed groove of the air jet loom for the weft insertion includes the jet stream from the relay nozzle, the back leak stream from the reed, and the diffusion stream from the open side. The air velocity in the reed groove can be measured to analyze the influence of process parameters during the weft insertion. The relation between the air velocity and the relay nozzle distance, the jet angle or the supply pressure of the relay nozzle can be studied. In this paper, the pitot tube is applied to measure the air velocity in reed groove by the instrument designed. And the results indicate that the air velocity fluctuation in the reed groove is strong, and can drop from 150 m·s-1 to 80 m·s-1 drastically.

关键词： air-jet loom reed groove airflow state relay nozzle weft insertion

Supercritical Fluid Carbon Dioxide in the Extraction of Lanolin and Its Alcohol

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Journal of Donghua University(English Edition) 2003年第1期20卷 105-108页

作者：林苗陈小立杨勇 College of Chemistry & Chemical Engineering Donghua University Shanghai 200051 inspection Center of Industrial Product & Raw Materials Shanghai Entry-Exit Inspection & Quarantine Bureau Shanghai 200135

The use of supercritical fluid carbon dioxide (SFCO2) in extraction of lanolin and its alcohol is superior to the conventional solvent extraction method. Its distinctive advantages include high extractive ratio, nontoxic and nonflammable solvents, and minimal by -product pollution. The resulting refined lanolin and its alcohol have light color and little odor, and can be used as raw materials for high grade cosmetic products.

关键词： supercritical fluid carbon dioxide (SF - CO_2) extraction lanolin lanolin alcohol acid value iodine value.

A new visual investigation into nanogold-based genechip assay by atomic force and scanning tunneling microscope

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Chinese Optics Letters 2010年第10期8卷 964-965页

作者：顾大勇谢伟东李震鲁卫平周元国季明辉 Research Institute of Disease Control and Prevention Shenzhen Internation Travel Healthcare Center Shenzhen Entry-Exit Inspection and Quarantine Bureau Life Science Division Graduate School at Shenzhen Tsinghua University Department of Chemical Defence Navy Submarine Academy PLA Department 7 Research Institute of Surgery and Daping Hospital The Third Military Medical University Research Institute of Disease Control and Prevention Shenzhen Entry-Exit Inspection and Quarantine Bureau

Conformations of surface atoms in various stages of nanogold-based genechip testing are scanned by the atomic force and scanning tunneling microscope. We intuitively observe the process and differences in probe combination, nucleic acid hybridization, and silver staining, which might be useful to validate the assay method of genechip. We hope to use this technology to make the other invisible chemical or biochemical reaction become visible and convincible in the future.

关键词： Microscopes Nucleic acids Surface testing