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Transcriptome analysis reveals key differentially expressed genes involved in wheat grain development

The Crop Journal 2016年第2期4卷 92-106页

作者： Yonglong Yu Dong Zhu Chaoying Ma Hui Cao Yaping Wang Yanhao Xu Wenying Zhang Yueming Yan College of Life Sciences Capital Normal University hubei collaborative innovation Center for grain industry/Yangtze University

Wheat seed development is an important physiological process of seed maturation and directly affects wheat yield and quality. In this study, we performed dynamic transcriptome microarray analysis of an elite Chinese bread wheat cultivar(Jimai 20) during grain development using the Gene Chip Wheat Genome Array. grain morphology and scanning electron microscope observations showed that the period of 11–15 days post-anthesis(DPA) was a key stage for the synthesis and accumulation of seed starch. Genome-wide transcriptional profiling and significance analysis of microarrays revealed that the period from 11 to 15 DPA was more important than the 15–20 DPA stage for the synthesis and accumulation of nutritive *** test of cluster analysis of differential genes revealed five statistically significant gene expression profiles. Gene ontology annotation and enrichment analysis gave further information about differentially expressed genes, and Map Man analysis revealed expression changes within functional groups during seed development. Metabolic pathway network analysis showed that major and minor metabolic pathways regulate one another to ensure regular seed development and nutritive reserve accumulation. We performed gene co-expression network analysis to identify genes that play vital roles in seed development and identified several key genes involved in important metabolic pathways. The transcriptional expression of eight key genes involved in starch and protein synthesis and stress defense was further validated by q RT-PCR. Our results provide new insight into the molecular mechanisms of wheat seed development and the determinants of yield and quality.

关键词： Wheat Transcriptome microarray Differentially expressed genes grain development

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Piriformospora indica confers drought tolerance on Zea mays *** enhancement of antioxidant activity and expression of drought-related genes

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The Crop Journal 2017年第3期5卷 251-258页

作者： Le Xu Aiai Wang Jun Wang Qiao Wei Wenying Zhang hubei collaborative innovation Center for grain industry/Research Center of Crop Stresses Resistance Technologies Yangtze UniversityJingzhou 434025China

Drought stress is one of the most severe environmental constraints to plant growth and crop productivity. Plant growth is greatly affected by drought stress, and plants, to survive,adapt to this stress by invoking different pathways. Piriformospora indica, a root-colonizing endophytic fungus of Sebacinales, promotes plant growth and confers resistance to biotic and abiotic stresses, including drought stress, by affecting the physiological properties of the host plant. The fungus strongly colonizes the roots of maize(Zea mays L.) and promotes shoot and root growth under both normal growth conditions and drought stress. We used polyethylene glycol(PEG-6000) to mimic drought stress and found that root fresh and dry weight, leaf area, SPAD value, and leaf number were increased in P. indica-colonized *** antioxidative activities of catalases and superoxide dismutases were upregulated within 24h in the leaves of P. indica-colonized plants. Drought-related genes DREB2A, CBL1,ANAC072, and RD29A were upregulated in drought-stressed leaves of P. indica-colonized plants. Furthermore, after drought treatment, proline content increased, whereas accumulation of malondialdehyde(MDA), an indicator of membrane damage, decreased in P. indica-colonized maize. We conclude that P. indica-mediated plant protection against the detrimental effects of drought may result from enhanced antioxidant enzyme activity,proline accumulation, and expression of drought-related genes and lower membrane damage in maize plants.

关键词： Antioxidants Drought-related genes Drought tolerance Piriformospora indica Maize

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The CCT domain-containing gene family has large impacts on heading date, regional adaptation, and grain yield in rice

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Journal of Integrative Agriculture 2017年第12期16卷 2686-2697页

作者： ZHANG Jia HU Yong XU Li-he HE Qin FAN Xiao-wei XING Yong-zhong National Key Laboratory of Crop Genetic Improvement/hubei collaborative innovation Center for grain industry HuazhongAgricultural University Wuhan 430070 P.R. China

There are 41 members of the CCT（CO, CO-like, and TOC1） domain-containing gene family in rice, which are divided into three subfamilies： COL（CONSTANS-like）, CMF（CCT motif family）, and PRR（pseudoresponse regulator）. The first flowering gene to be isolated by map-based cloning, Heading date 1（Hd1）, which is the orthologue of CO in rice, belongs to COL. The central regulator of plant development, Ghd7, belongs to CMF. The major role in controlling rice distribution to high latitudes, Ghd7.1/PRR37, belongs to PRR. Both of Hd1, Ghd7 and Ghd7.1 simultaneously control grain number, plant height, and the heading date. To date, 13 CCT family genes from these three subfamilies have been shown to regulate flowering. Some of them have pleiotropic effects on grain yield, plant height, and abiotic stresses, and others function as circadian oscillators. There are two independent photoperiod flowering pathways that are mediated by GI-Hd1-Hd3 a/RFT and GI-Ehd1-Hd3 a/RFT in rice. CCT family genes are involved in both pathways. The latest study reveals that protein interaction between Hd1 and Ghd7 integrates the two pathways. CCT family genes are rich in natural variation because rice cultivars have been subjected to natural and artificial selection for different day lengths in the process of domestication and improvement. Alleles of several crucial CCT family genes such as Hd1, Ghd7, and Ghd7.1 exhibit geographic distribution patterns and are highly associated with yield potentials. In addition, CCT family genes are probably involved in the responses to abiotic stress, which should be emphasized in future work. In general, CCT family genes play important roles in regulating flowering, plant growth, and grain yield. The functional identification and elucidation of the molecular mechanisms of CCT family genes would help construct a flowering regulatory network and maximize their contribution to rice production.

关键词： photoperiod sensitivity protein interaction yield potential geographic distribution abiotic stress

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Chlorogenic Acid Metabolism:The Evolution and Roles in Plant Response to Abiotic Stress

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Phyton-International Journal of Experimental Botany 2022年第2期91卷 239-255页

作者： Deka Reine Judesse Soviguidi Rui Pan Yi Liu Liping Rao Wenying Zhang Xinsun Yang Engineering Research centre of Ecology and Agricultural Use of Wetland Ministry of EducationHubei Collaborative Innovation Center for Grain IndustryYangtze UniversityJingzhou434025China hubei Sweetpotato Engineering and Technology Research centre Hubei Academy of Agricultural SciencesWuhan430064China

During the evolution,plants acquired the ability to synthesize different phenylpropanoid compounds like chlorogenic acid(CGA),which plays vital roles in resistance mechanisms to abiotic *** environmental factors,including heavy metal,cold,heat,ultraviolet(UV)light,drought,and salinity affect the plant physiological processes,resulting in massive losses of agriculture *** plants evolve from green algae to bryophytes,ferns,gymnosperms and angiosperms,phenylpropanoids are produced and accumulated in different tissues,giving the plant the capacity to counteract the harmful effects of the adverse *** have been performed on the metabolic evolution of rosmarinic acid,flavonoids and lignin,showing that the biosynthesis of phenylpropanoids begins in green algae until the emersion of genes found in angiosperms;however,the evolution of the CGA pathway has not yet been *** hypothesize that CGA could also be synthesized from algae to *** the present review,the evolutionary analysis of CGA pathway and the function of this compound in plant tolerance to abiotic stresses are *** analyzes were carried out on CGA-related genes across 37 plant species and revealed that the metabolic pathway starts in algae and gradually increases until it becomes complete in *** key genes exhibited different expression patterns in stress and plant ***,some genes accumulated rapidly during evolution and were more sensitive to environmental stresses,while others appeared only later in *** studies are needed to better understand the evolution of the CGA metabolic pathway in plants under environmentally stressed conditions.

关键词： Chlorogenic acid biosynthesis evolution plant abiotic stress

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Bio-inspired Hydroxyapatite/Gelatin Transparent Nanocomposites

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Journal of Wuhan University of Technology(Materials Science) 2024年第2期39卷 298-308页

作者：谭军军 WU Mingchen LI Yuzhe PENG Jiamei 熊焰 hubei Province Key Laboratory of Green Materials for Light industry Collaborative Innovation Center for Green Light-weight Materials and ProcessingHubei University of TechnologyWuhan 430068China

Hydroxyapatite(HA)nanoparticles impart outstanding mechanical properties to organicinorganic nanocomposites in *** by the composite structure of HA nanoparticles and collagen in bone,a high performance HA/gelatin nanocomposite was first *** nanocomposites have much better mechanical properties(elongation at break 29.9%,tensile strength 90.7 MPa,Young’s modulus 5.24 GPa)than pure gelatin films(elongation at break 9.3%,tensile strength 90.8 MPa,Young’s modulus 2.5 GPa).In addition,the composite films keep a high transmittance in visible wavelength range from 0%to 60%of the HA solid *** differences in properties are attributed to the homogeneous distribution of HA nanoparticles in the gelatin polymer matrix and the strong interaction between the particle surfaces and the gelatin *** protocol should be promising for HA-based nanocomposites with enhanced mechanical properties for biomedical applications.

关键词： hydroxyapatite nanocomposites sodium citrate gelatin colloidal stability

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grain proteomic analysis reveals central stress responsive proteins caused by wheat-Haynaldia villosa 6VS/6AL translocation

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Journal of Integrative Agriculture 2020年第11期19卷 2628-2642页

作者： ZOU Rong WU Ji-su WANG Ruo-mei YAN Yue-ming College of Life Science Capital Normal UniversityBeijing 100048P.R.China hubei collaborative innovation Center for grain industry(HCICGI) Yangtze UniversityJingzhou 434023P.R.China

Haynaldia villosa(2n=14,VV),a wild grass of the subtribe Triticeae,serves as potential gene resources for wheat genetic *** this study,the proteome characterization during grain development of Yangmai 5 and Yangmai *** 6VS/6AL translocation line was investigated by a comparative proteomic ***-dimensional electrophoresis identified 81 differentially accumulated proteins(DAPs)during five grain developmental stages in *** translocation *** proteins were mainly involved in stress defense,storage protein,energy metabolism,protein metabolism and folding,carbon metabolism,nitrogen metabolism,and starch *** particular,6VS/6AL translocation led to significant upregulation of 36 DAPs and specific expression of 11 DAPs such as chitinase,thaumatin-like proteins,glutathione transferase,α-amylase inhibitor,heat shock proteins,and betaine aldehyde *** proteins mainly involved in biotic and abiotic stress *** analysis found that the upstream 1500 promoter regions of these stress-responsive DAP genes contained multiple high-frequency cis-acting elements related to stress defense such as abscisic acid response element ABRE,methyl jasmonate(MeJA)-response element TGACG-motif and CGTCA-motif involved in oxidative stress and antioxidant response element(ARE).RNA-seq and RT-qPCR analyses revealed the high expression of these stress-defensive DAP genes in the developing grains,particularly at the early-middle grain filling *** results demonstrated that 6VS chromosome of *** contains abundant stress-defensive proteins that have potential values for wheat genetic improvement.

关键词： 6VS/6AL translocation grain development proteome DAPs cis-acting elements adverse defense

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Why high grain yield can be achieved in single seedling machine-transplanted hybrid rice under dense planting conditions?

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Journal of Integrative Agriculture 2018年第6期17卷 1299-1306页

作者： HUANG Min SHAN Shuang-lü XIE Xiao-bing CAO Fang-bo ZOU Ying-bin Southern Regional collaborative innovation Center for grain and Oil Crops (CICGO) Hunan Agricultural University

This study was conducted to identify the factors associated with high grain yield in single seedling machine-transplanted hybrid rice under dense planting conditions. Field experiments were done in Yong＇an Town, Hunan Province, China in 2015 and 2016. Two hybrid rice cultivars were grown under single seedling machine transplanting （SMT） and conventional machine transplanting （CMT） at a high planting density in each year. grain yield and yield attributes were compared between SMT and CMT. Averaged across cultivars and years, grain yield was 12％ higher under SMT than under CMT. Plant height, basal stem width, and shoot and root dry weights were higher in seedlings for SMT than those for CMT. SMT had less maximum tiller number per m2 and consequently less panicle number per m2 than did CMT. Branch number per panicle, especially the secondary branch number per panicle, and spikelet number per cm of panicle length were more under SMT than under CMT, which resulted in more spikelet number per panicle under SMT than under CMT. SMT had higher or equal spikelet filling percentage than did CMT. The difference in grain weight between SMT and CMT was relatively small and inconsistent cross years. SMT had higher or equal total biomass and harvest index than did CMT. Dry weight per stem under SMT was heavier than that under CMT. Larger leaf area per stem was partly responsible for the heavier dry weight per stem under SMT than under CMT. Our study suggests that improvement in seedling quality, panicle size, and dry weight per stem are critical to the high grain yield in single seedling machine-transplanted hybrid rice under dense planting conditions.

关键词： grain yield hybrid rice machine transplanting single seedling

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Harness the power of genomic selection and the potential of germplasm in crop breeding for global food security in the era with rapid climate change

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The Crop Journal 2020年第5期8卷 688-700页

作者： Tianhua He Chengdao Li Western Barley Genetics Alliance Western Australian State Agricultural Biotechnology CentreCollege of ScienceHealthEngineering and EducationMurdoch UniversityMurdochWA 6150Australia Agriculture and Food Department of Primary Industries and Regional DevelopmentSouth PerthWA 6151Australia hubei collaborative innovation centre for grain industry Yangtze UniversityJingzhou 434025HubeiChina

Crop genetic improvements catalysed population growth,which in turn has increased the pressure for food *** need to produce 70%more food to meet the demands of 9.5 billion people by *** changes have posed challenges for global food supply,while the narrow genetic base of elite crop cultivars has further limited our capacity to increase genetic gain through conventional *** effective utilization of genetic resources in germplasm collections for crop improvement is crucial to increasing genetic gain to address challenges in the global food *** selection(GS)uses genome-wide markers and phenotype information from observed populations to establish associations,followed by genome-wide markers to predict phenotypic values in test *** an extensive germplasm collection can serve a dual purpose in GS,as a reference population for predicting model,and mining desirable genetic variants for incorporation into elite *** technologies,such as high-throughput genotyping and phenotyping,machine learning,and gene editing,have great potential to contribute to genomeassisted *** programmes integrating germplasm characterization,GS and emerging technologies offer promise for accelerating the development of cultivars with improved yield and enhanced resistance and tolerance to biotic and abiotic ***,scientifically informed regulations on new breeding technologies,and increased sharing of genetic resources,genomic data,and bioinformatics expertise between developed and developing economies will be the key to meeting the challenges of the rapidly changing climate and increased demand for food.

关键词： breeding germplasm cultivar

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Evaluation of the intracellular lipid-lowering effect of polyphenols extract from highland barley in HepG2 cells

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Food Science and Human Wellness 2024年第1期13卷 454-461页

作者： Yijun Yao Zhifang Li Bowen Qin Xingrong Ju Lifeng Wang College of Food Science and Engineering/collaborative innovation centre for Modern grain Circulation and Safety Nanjing University of Finance and EconomicsNanjing 210023China

Active ingredients from highland barley have received considerable attention as natural products for developing treatments and dietary supplements against *** practical application,the research of food combinations is more significant than a specific food *** study investigated the lipid-lowering effect of highland barley polyphenols via lipase assay in vitro and HepG2 cells induced by oleic acid(OA).Five indexes,triglyceride(TG),total cholesterol(T-CHO),low density lipoprotein-cholesterol(LDL-C),aspartate aminotransferase(AST),and alanine aminotransferase(ALT),were used to evaluate the lipidlowering effect of highland barley *** also preliminary studied the lipid-lowering mechanism by Realtime fluorescent quantitative polymerase chain reaction(q PCR).The results indicated that highland barley extract contains many components with lipid-lowering effects,such as hyperoside and *** vitro,the lipase assay showed an 18.4%lipase inhibition rate when the additive contents of highland barley extract were 100μg/m *** intracellular lipid-lowering effect of highland barley extract was examined using 0.25 mmol/L OA-induced HepG2 *** results showed that intracellular TG,LDL-C,and T-CHO content decreased by 34.4%,51.2%,and 18.4%,*** and AST decreased by 51.6%and 20.7%compared with the untreated hyperlipidemic HepG2 cells.q PCR results showed that highland barley polyphenols could up-regulation the expression of lipid metabolism-related genes such as PPARγand Fabp4.

关键词： Highland barley Polyphenols extract Lipid-lowering effect HepG2 cells

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Improving nitrogen management for zero-tillage rice in China

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The Crop Journal 2018年第4期6卷 406-412页

作者： Min Huang Xuefeng Zhou Yingbin Zou Southern Regional collaborative innovation Center for grain and Oil Crops (CICGO) Hunan Agricultural University

Zero-tillage has become increasingly attractive in rice production in *** study was conducted to determine the feasibility of two possible improved N management practices with fewer N applications in zero-tillage rice:(1)two split applications of urea at75 kg N ha^(-1)at mid-tillering and 45 kg N ha^(-1)at panicle initiation(U_(120–2)),and(2)a single application of cross-linked polyacrylamide-coated urea(a slow-release fertilizer)at midtillering at a rate of 150 kg N ha^(-1)(PCU_(150–1)).Three field experiments were conducted to compare grain yield and N-use efficiency among several N treatments:a zero-N control(CK),U_(120–2),PCU_(150–1),a single application of urea at mid-tillering at a rate of 150 kg N ha^(-1)(U_(150–1)),and a commonly recommended N management practice for conventional tillage rice(three split applications of urea with 75 kg N ha^(-1)as basal,30 kg N ha^(-1)at mid-tillering,and 45 kg N ha^(-1)at panicle initiation)(U_(150–3)).Treatments with N application(U_(120–2),PCU_(150–1),U_(150–1),and U_(150–3))produced 1.08–3.16 t ha^(-1)higher grain yields than *** yields under both U_(120–2)and PCU_(150–1)were comparable to that in U_(150–3).Recovery efficiency of N(RE_N),agronomic N-use efficiency(AE_N)and partial factor productivity of applied N(PFP_N)were increased under U_(120–2)and were similar under PCU_(150–1)to those under U_(150–3).U_(150–1)showed lower grain yield,RE_N,AE_N,and PFP_Nthan U_(150–3).These results suggest that U_(150–3)can be replaced with U_(120–2)to achieve both an increase in N-use efficiency and a reduction in number of N applications and or by PCU_(150–1)to achieve a maximum reduction in number of N applications in zero-tillage rice production in China.

关键词： grain yield N management N-use efficiency Rice Zero-tillage

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