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A new species and two new records of Ulocladium from China

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菌物学报 2008年第1期27卷 1-4页

作者： LIU Hui-Mei ZHANG Tian-Yu Department of Plant Pathology Shandong Agricultural University Tai＇ an 271018 China huangdao entry-exit inspection and quarantine bureau Qingdao 266555 China

A new species and two new Chinese records in the genus Ulocladium from soil are reported. New species is Ulocladium leve, and the new records are U. alternariae and U. consortiale. Latin diagnosis of the new species is presented. Holotype specimen (dried culture) of the new species and dried cultures of two new records are deposited in the Herbarium of Shandong Agricultural University: Plant Pathology (HSAUP).

关键词：土壤真菌中国新种新记录生物学特征暗色丝孢菌

Developmental expression amphioxus Branchiostoma pattern of calmodulin gene in belcheri tsingtauense

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Chinese Journal of Oceanology and Limnology 2010年第6期28卷 1221-1226页

作者： LUAN Jing GENG Jinpei FANG Shaoqing SU Zhiping ZHANGJingxuan XU Hongyan WANGYing LUMin YIN Weili MENG Guangxiao Department of Marine Biology Ocean university of China Qingdao 266003 China Yantai entry-exit inspection and quarantine bureau Yantai 264000 China

We investigated the developmental expression pattern of AmphiCaM in cephalochordate amphioxus （Branchiostoma belcheri tsingtauense）. We cultured and sampled the animals at different developmental stages （eggs and larvae）, and used in-situ hybridization and northern blotting to document the spatial and temporal changes in AmphiCaM expression. The alimentary tract dominates the development from the late neurula stage to the adult stage. AmphiCaM expression increased significantly in the alimentary tract during the late neurula stage and remained elevated in the adults. Our results indicate that AmphiCaM is involved in the differentiation of the alimentary tract in amphioxus; and furthermore, provide an insight into the change in function of CaM genes during evolution.

关键词： amphioxus calmodulin expression evolution

Application and safety assessment for nano-composite materials in food packaging

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Chinese Science Bulletin 2011年第12期56卷 1216-1225页

作者： HAN Wei YU YanJun LI NingTao WANG LiBing The Hazardous Products Central Laboratory General Administration of Quality Supervision Inspection and Quarantine of the People's Republic of China Tianjin 300042 China Technical Center for Safety of lndustrial Products Tianjin Entry-Exit Inspection and Quarantine Bureau of China Tianjin 300042 China Hunan entry-exit inspection and quarantine bureau of China Changsha 410004 China

The technical breakthroughs and rapid developments in nanotechnologies enabling goods and products based on nanomaterials (NMs) has come to media and public attention as potentially one of the most significant technological advances of our time. Among these technical applications, nano-composite food packaging, which combines the NMs and conventional packaging, is at the forefront of applications in nanotechnologies, leading the whole industrial chain based on nanotechnologies with high speed development. Taking into account the status of the nano-composites applied in food packaging and present development trends, this review is focused on summarizing the basic applications of the nano-composites in food packaging as well as its risk assessment. The status summaries and the conclusions derived from this review are of benefit to manufacturers which produce the nano-composites used in food packaging, to general consumers and to governmental administration entities.

关键词：纳米复合材料食品包装应用安全评估纳米技术纳米材料技术进步发展趋势

Diatom diet selectivity by early post-larval abalone Haliotis diversicolor supertexta under hatchery conditions

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Chinese Journal of Oceanology and Limnology 2010年第6期28卷 1187-1194页

作者：张玉宇高亚辉梁君荣陈长平赵东海李雪松李扬吴文忠 Key Laboratory of Ministry of Education for Coast and Wetland Ecosystems and School of Life Sciences Xiamen University Dongshan entry-exit inspection and quarantine bureau

Benthic diatoms constitute the primary diet of abalone during their early stages of development. To evaluate the dietary preferences of early post-larval abalone, Haliotis diversicolor supertexta, we analyzed the gut contents of post-larvae that settled on diatom films. We compared the abundance and species diversity of diatom assemblages in the gut to those of the epiphytic diatom assemblages on the attachment films, and identified 40 benthic diatom species in the gut contents of post-larvae 12 to 24 d after settlement. The most abtmdant taxa in the gut contents were Navicula spp., Amphora copulate, and Amphora coffeaeformis. Navicula spp. accounted for 64.0% of the cell density. In the attachment films, we identified 110 diatom species belonging to 38 genera. Pennate diatoms were the dominant members including the species Amphiprora alata, Cocconeis placentula var. euglypta, Cylindrotheca closterium, Navicula sp. 2, and A. coffeaeformis. Nano-diatoms （〈20 pm in length） accounted for a considerable proportion of the total species number and cell density of the diatom assemblages in the gut contents and on the films. This suggests that nano-diatoms are important to the efficient production of abalone seed. The difference of the composition and abundance of diatoms between in the guts and on the biofilms suggests that early post-larval grazing was selective. An early post-larval abalone preferred nano-diatoms and the genera Navicula and Amphora during the month after settlement.

关键词： benthic diatoms feeding Haliotis diversicolor supertexta post-larval abalone

High hydrosilylation efficiency of porous silicon SiHx species produced by Pt-assisted chemical etching for biochip fabrication

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Science China Chemistry 2013年第8期56卷 1152-1163页

作者： XIAO MinYu HAN HuanMei XIAO ShouJun State Key Laboratory of Coordination Chemistry School of Chemistry and Chemical EngineeringNanjing National Laboratory of MicrostructuresNanjing University Jinan entry-exit inspection and quarantine bureau inspection

Porous silicon （PSi） prepared from Pt metal-assisted chemical etching （MACE） was demonstrated to possess higher hydrosi- lylation efficiency （-57%） than anodized PSi （-11%） by surface reaction with co-undecenyl alcohol （UO）. Deconvolution of the SiHx （x = 1-3） stretching bands revealed the abundance of SiH2 species on MaCE PSi was 53%, -10% higher than on ano- dized samples, while both of Sill1 and Sill3 were -5% lower correspondently on MaCE PSi than on anodized samples. The surface SiHx abundances were suggested to account for the higher hydrosilylation efficiency on MaCE PSi. Optimization of Pt-assisted chemical etching parameters suggested a 7-15 nm thick Pt-coating and an etching time of 3-10 min for biochip ap- plications. Scanning electron microscopy images revealed that an isotropic top meso-porous layer was beneficial for hydrosi- lylation and long-term durability under ambient conditions. To end, an example of histidine-tagged protein immobilization and microarray was illustrated. Combining the materials＇ property, surface chemistry, and micro-fabrication technology together, we envision that silicon based biochip applications have a prosperous future.

关键词： metal-assisted chemical etching porous silicon surface chemistry hydrosilylation biochip

Fast Evaluation of Oxidative DNA Damage by Liquid Chromatography-Electrospray Tandem Mass Spectrometry Coupled With Precision-cut Rat Liver Slices

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Biomedical and Environmental Sciences 2007年第5期20卷 386-391页

作者： JIANG YUE PENG WANG YING-HUI LIU JUN-YU WU JIE CHEN REN-XIU PENG Department of Pharmacology College of Basic Medicine Wuhan University Wuhan 430071 Hubei China inspection ＆ quarantine Technical Center Hubei Entry-exit Inspection and Quarantine Bureau Wuhan 430022 Hubei China

Objective To establish a fast and sensitive method for the detection of 8-hydroxy-2’-deoxyguanosine （8-OHdG） in precision-cut rat liver slices by HPLC-MS/MS and to investigate isoniazid （INH） -induced oxidative DNA damage. Methods Precision-cut liver slices （300 μm） were prepared from male rats, and incubated with INH （0.018 mol/L） for 2 h after 1 h preincubation. DNA in the slices was extracted and digested into free nucleosides at 37℃ . The samples were injected into HPLC-MS/MS after the proteins were removed. The level of oxidative DNA damage was estimated using the ratio of 8-OHdG to deoxyguanosine （dG）. Results The limit of detection of 8-OHdG was 1 ng/mL （S/N=3） and the intra-assay relative standard variation was 3.38% when one transition 284.3/168.4 was used as a quantifier and another two transitions 284.3/140.2, 306.1/190.2 as qualifiers. 8-OHdG and dG were well separated, as indicated by elution at 10.02 and 7.37 min, respectively. INH significantly increased the ratio of 8-OHdG to dG in rat liver slices （P〈0.05）. Conclusion 8-OHdG in precision-cut liver slices could be sensitively determined by HPLC-MS/MS. HPLC-MS/MS coupled with precision-cut tissue slices is a fast and reliable analytical technique to evaluate oxidative DNA damage of target tissues caused by procarcinogens and cytotoxins.

关键词： Isoniazid 8-Hydroxy-2'-deoxyguanosine HPLC-MS/MS Precision-cut liver slices

Comparison and Analysis of Fabric Deodorization Test Methods

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Journal of Donghua University(English Edition) 2012年第3期29卷 268-271页

作者：魏孟媛薛文良陆维民马洁陈智菲 Shanghai entry-exit inspection and quarantine bureau Engineering Research Center of Technical Textiles Ministry of EducationDonghua University

The test methods of deodorization fabric are studied in daily life. In view of different deodorization fabrics and source gases,the detector tube method and gas chromatography-flame ionization detector ( GC-FID ) method were chosen to test the deodorizing rates of fabric and the influencing factors on deodorization test were discussed. Through the two methods,the residual concentration of odorous substance after adsorption can be measured,and the deodorizing rates of deodorization fabric can be got by calculation. The results show that the two test methods are both appropriate for deodorization test of fabric.

关键词： deodorization fabric deodorizing rate detector tube gas chromatoeraphy-flame ionization detector (GC-FID)

Quantitative Risk Assessment with Data Mining for Formaldehyde Content of Imported Textile

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Journal of Donghua University(English Edition) 2014年第4期31卷 437-440页

作者：薛文良胡向媛魏孟媛马哲远钱竞芳 Key Laboratory of Textile Science & Technology Ministry of EducationDonghua University Shanghai entry-exit inspection and quarantine bureau

The assessment of potential risks posed by formaldehyde in clothing to consumers is of increasing concern worldwide,for free formaldehyde is harmful to human. Some papers focus on hazard and exposure assessment,using quantitative and semi-quantitative method, rather than assessment for the factors related to formaldehyde content. In order to perform early-warning research and risk management,the quantitative risk assessment is used to analyze the imported textile testing data and find the factors related to formaldehyde content. K-means algorithm is the core of the method. It can be concluded that the disqualified textiles have relations with main fiber components,fabric color,season month,and brand. Then the data in the same attribute are clustered by Kmeans algorithm. The different classes are defined as related danger level,respectively severe,moderate,and light.

关键词： quantitative risk assessment formaldehyde content disqualified textiles

Additional Twists of Yarn Produced by System of Compact Spinning with Lattice Apron

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Journal of Donghua University(English Edition) 2011年第5期28卷 507-510页

作者：薛文良魏孟媛程隆棣钱竞芳 Key Laboratory of Textile Science & Technology Ministry of EducationDonghua University Shanghai entry-exit inspection and quarantine bureau

Based on the flow simulation in the condensing zone of compact spinning with lattice apron and a bead-elastic rod dynamic model of the flexible fiber,trajectories of fibers with different negative pressure are simulated by specially designed Matlab *** displacement components of fibers at YZ profile under different negative pressure conditions are extracted and *** results show that the fibers of different initial positions gradually converge,and are interlaced for position change in yarn cross-section,caused by the airflow in the condensing ***,compact-spun yarn with different negative pressure and conventional ring spun yarn are produced and their twists are *** the results of simulation and experiments illustrate the existence of additional *** the relationship between additional twists and negative pressure is verified.

关键词： compact spinning with lattice apron simulation dynamical model trajectory additional twists