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Nigella sativa L. seed extract alleviates oxidative stress-induced cellular senescence and dysfunction in melanocytes

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Chinese Journal of Natural Medicines 2025年第02期23卷 203-213页

作者： Ben Niu Xiaohong An Yongmei Chen Ting He Xiao Zhan Xiuqi Zhu Fengfeng Ping Wei Zhang Jia Zhou School of Traditional Chinese Pharmacy China Pharmaceutical University Wuxi People's hospital Nanjing Medical University Yunnan Characteristic Plant Extraction Laboratory Yunnan Yunke Characteristic Plant Extraction Laboratory Co. Ltd. Drug Discovery and Development Laboratories Ningxia Hui Medicine Research Institute hospital for skin diseases institute of dermatology Chinese Academy of Medical Sciences & Peking Union Medical College

Nigella sativa L. seeds have been traditionally utilized in Chinese folk medicine for centuries to treat vitiligo. This study revealed that the ethanolic extract of Nigella sativa L.(HZC) enhances melanogenesis and mitigates oxidative stress-induced cellular senescence and dysfunction in melanocytes. In accordance with established protocols, the ethanol fraction from Nigella sativa L. seeds was extracted, concentrated, and lyophilized to evaluate its herbal effects via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assays, tyrosinase activity evaluation, measurement of cellular melanin contents, scratch assays, senescence-associated β-galactosidase(SA-β-gal) staining, enzyme-linked immunosorbent assay(ELISA), and Western blot analysis for expression profiling of experimentally relevant proteins. The results indicated that HZC significantly enhanced tyrosinase activity and melanin content while notably increasing the protein expression levels of Tyr, Mitf, and gp100 in B16F10 cells. Furthermore, HZC effectively mitigated oxidative stress-induced cellular senescence, improved melanocyte condition, and rectified various functional impairments associated with melanocyte dysfunction. These findings suggest that HZC increases melanin synthesis in melanocytes through the activation of the MAPK, PKA, and Wnt signaling *** addition, HZC attenuates oxidative damage induced by H2O2 therapy by activating the nuclear factor E2-related factor 2-antioxidant response element(Nrf2-ARE) pathway and enhancing the activity of downstream antioxidant enzymes, thus preventing premature senescence and dysfunction in melanocytes.

关键词： Cellular senescence Melanin Nigella sativa L. seed extract (HZC) ROS Vitiligo

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The comprehensive assessment of epigenetics changes during skin development

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Fundamental Research 2025年第1期5卷 228-240页

作者： Li Lei Ling Jiang Yibo Hu Menglu Chen Jinhua Huang Jing Chen Qinghai Zeng Department of dermatology Third Xiangya HospitalCentral South UniversityChangsha 410013China

Epigenetic regulation is critical to multiple physiological and pathological ***,little is known regarding the epigenetic changes during neonatal skin development and skin aging,and in response to ultraviolet(UV)*** transcriptomes of human skin samples from different ages or irradiated with different types and doses of UV light were analyzed using R(version 4.0.3)*** epigenetic landscape of the skin,including histone modifications,genetic imprinting and m^(6)A modification,which are mainly involved in collagen formation,extracellular matrix organization,immune function and keratinization,underwent significant changes during neonatal to adult *** effectors such as IGF2BP2,GATA2,GATA3,CPA4 and CDK1 were significantly correlated with extracellular matrix organization,and VEGFA,CDK1 and PRKCB with skin immune *** m^(6)A readers such as IGF2BP2,IGF2BP3,HNRNPA2B1 and EIF3G showed significant correlation with extracellular matrix organization,metabolism,or antigen processing and *** doses of UV exposure only induced changes in the expression levels of some epigenetic effectors,without any significant effect on the overall epigenetic ***,the minimal erythema dose of UV exposure altered multiple epigenetic effectors regulating extracellular matrix organization,cell-matrix adhesion,innate immune response,mitochondrial function and mRNA *** addition,epigenetic changes following UV exposure were more pronounced in the elderly skin compared to the younger *** conclusion,histone modifications,genetic imprinting and m^(6)A modification play critical roles during skin development,and a large dose of UV exposure can significantly change the expression of multiple epigenetic effectors.

关键词： Epigenetics skin development Aging Ultraviolet m^(6)A

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A dynamic peripheral immune landscape during human pregnancy

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Fundamental Research 2025年第1期5卷 391-406页

作者： Xiuxing Liu Lei Zhu Zhaohao Huang Zhaohuai Li Runping Duan He Li Lihui Xie Xiaozhen Chen Wen Ding Binyao Chen Yuehan Gao Juan Su Xianggui Wang Wenru Su State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterGuangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceSun Yat-Sen UniversityGuangzhou 510060China Eye Center of Xiangya hospital Central South UniversityChangsha 410078China Hunan Key Laboratory of skin Cancer and Psoriasis Xiangya HospitalCentral South UniversityChangsha 410008China Guangzhou Women and Children's Medical Center Guangzhou Medical UniversityGuangzhou 510623China Department of dermatology Xiangya HospitalCentral South UniversityChangsha 410008China Hunan Engineering Research Center of skin Health and Disease Central South UniversityChangsha 410008China National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangsha 410008China Hunan Key Laboratory of Ophthalmology Changsha 410078China

Extensive immune adaptations occur during pregnancy to ensure successful ***,these changes can increase the risk of disease in the ***,we conducted single-cell RNA sequencing on peripheral blood mononuclear cells from pregnant women at different stages of pregnancy to elucidate the dynamic transcriptional changes in the maternal immune *** reduced cytotoxicity phenotype in highly variable cytotoxic T and natural killer cell types were observed during *** T-and B-cell response-related MHC-II and CD40 signaling as well as enhanced protolerance inducible costimulator and activin signaling may underlie the pregnancy-related weakening of adaptive ***,pro-inflammatory genes and pathways were upregulated in monocytes,possibly to compensate for the reduced T-cell ***,the transition from adaptive immune reduction to activation in late pregnancy in dendritic cells and CD4^(+)T cells was also ***,we proposed a novel view of the pro-aging effect of pregnancy from the perspective of immunity,and this effect may be restored *** work expands our knowledge of pregnancy immunity and may provide insights into the altered disease risks during pregnancy.

关键词： Single-cell RNA sequencing Pregnancy Maternal immune system Aging Immune tolerance Dampened adaptive immunity

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Differential expression of plasma extracellular vesicle miRNAs as biomarkers for distinguishing psoriatic arthritis from psoriasis

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中华医学杂志(英文版) 2025年第2期138卷 219-221页

作者： Yan Kexiang Zhu Jie Zhang Mengmeng Zhang Fuxin Wang Bing Han Ling Huang Qiong Tang Yulong Li Yuan Yawalkar Nikhil Zhang Zhenghua Niu Zhenmin Department of dermatology Huashan Hospital Fudan University Shanghai China State Key Laboratory of Genetic Engineering Collaborative Innovation Center for Genetics and Development School of Life Sciences Human Phenome Institute Fudan University Shanghai China Department of dermatology Inselspital Bern University Hospital University of Bern Bern CH-Bern Switzerland Department of Genetics Shanghai-MOST Key Laboratory of Health and Disease Genomics Chinese National Human Genome Center at Shanghai (CHGC) and Shanghai Academy of Science &amp Technology (SAST) Shanghai China

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Dystrophic epidermolysis bullosa caused by novel frameshift mutation in the COL7A1 gene: A case report

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World Journal of Clinical Cases 2025年第11期13卷 60-65页

作者： Yan Yang Zhi-Wei Guan Qin-Feng Li Department of Nursing Tianjin Children’s HospitalTianjin 300074China Department of dermatology Tianjin Children’s HospitalTianjin 300074China

BACKGROUND Dystrophic epidermolysis bullosa is characterized by fragile ulcerations of the skin caused by mutations in specific ***,genetic typing of this con-dition is *** SUMMARY An 11-year-old female suffered from recurrent fever,visible ulcerations of the entire skin,and severe *** testing revealed a frameshift mu-tation in the coding region 4047 of the 35th intron region of COL7A1,and she was diagnosed as malnutrition-type epidermolysis *** therapy(immu-noglobulin,fresh frozen plasma),topical therapy(silver ion dressing),fever redu-ction,cough relief,and promotion of gastrointestinal peristalsis are mainly used for respiratory and gastrointestinal *** patient’s condition impro-ved after *** Dystrophic epidermolysis bullosa caused by a new framework shift mutation in COL7A1 should be taken seriously.

关键词： Dystrophic epidermolysis bullosa Frameshift mutation Genetic testing COL7A1 gene Genetic typing Immunoglobulin Case report

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Recent advances in systemic lupus erythematosus and microbiota: from bench to bedside

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Frontiers of Medicine 2022年第5期16卷 686-700页

作者： Yijing Zhan Qianmei Liu Bo Zhang Xin Huang Qianjin Lu Key Laboratory of Basic and Translational Research on Immune-Mediated skin diseases Chinese Academy of Medical SciencesJiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIsInstitute of DermatologyChinese Academy of Medical Sciences and Peking Union Medical CollegeNanjing 210042China Department of dermatology The Second Xiangya Hospital of Central South UniversityInstitute of Dermatology and Venereology of Central South UniversityHunan Clinical Medicine Research Center for Major Skin Diseases and Skin HealthChangsha 410011China

Systemic lupus erythematosus(SLE)is a complicated autoimmune disease affecting multiple systems and *** is highly heterogeneous,and it preferentially affects women at childbearing age,causing worldwide social *** pathogenesis of SLE mostly involves genetic predisposition,epigenetic dysregulation,overactivation of the immune system,and environment *** microbiome,which is mostly composed of microbiota colonized in the gut,skin,and oral cavity,provides a natural microbiome barrier against environmental *** past decade of research has demonstrated a strong association between microbiota and metabolic diseases or gastrointestinal ***,the role of microbiota in autoimmunity remains largely unknown until recently,when the technological and methodological progress facilitates further microbiota research in *** this review,the latest research about the role and mechanisms of microbiota in SLE and the advances in the development of diagnostic and therapeutic strategies based on microbiota for SLE were summarized.

关键词： systemic lupus erythematosus microbiota biotherapy

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Antimicrobial resistance crisis:could artificial intelligence be the solution?

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Military Medical Research 2025年第1期12卷 72-95页

作者： Guang-Yu Liu Dan Yu Mei-Mei Fan Xu Zhang Ze-Yu Jin Christoph Tang Xiao-Fen Liu Department of Immunology and Pathogen Biology School of Basic Medical SciencesHangzhou Normal UniversityKey Laboratory of Aging and Cancer Biology of Zhejiang ProvinceKey Laboratory of Inflammation and Immunoregulation of HangzhouHangzhou Normal UniversityHangzhou 311121China National Key Discipline of Pediatrics Key Laboratory of Major diseases in Children Ministry of Education Laboratory of DermatologyBeijing Pediatric Research InstituteBeijing Children’s HospitalCapital Medical UniversityNational Center for Children’s HealthBeijing 100045China Robert and Arlene Kogod Center on Aging Mayo ClinicRochesterMN 55905USA Department of Biochemistry and Molecular Biology Mayo ClinicRochesterMN 55905USA Department of Molecular and Human Genetics Baylor College of MedicineHoustonTX 77030USA Sir William Dunn School of Pathology University of OxfordOxford OX13REUK institute of Antibiotics Huashan HospitalFudan UniversityKey Laboratory of Clinical Pharmacology of AntibioticsNational Health Commission of the People’s Republic of ChinaNational Clinical Research Centre for Aging and MedicineHuashan HospitalFudan UniversityShanghai 200040China

Antimicrobial resistance is a global public health threat,and the World Health Organization(WHO)has announced a priority list of the most threatening pathogens against which novel antibiotics need to be *** discovery and introduction of novel antibiotics are time-consuming and *** to WHO’s report of antibacterial agents in clinical development,only 18 novel antibiotics have been approved since ***,novel antibiotics are critically *** intelligence(AI)has been rapidly applied to drug development since its recent technical breakthrough and has dramatically improved the efficiency of the discovery of novel ***,we first summarized recently marketed novel antibiotics,and antibiotic candidates in clinical *** addition,we systematically reviewed the involvement of AI in antibacterial drug development and utilization,including small molecules,antimicrobial peptides,phage therapy,essential oils,as well as resistance mechanism prediction,and antibiotic stewardship.

关键词： Antibiotic Artificial intelligence(AI) Clinical development Machine learning(ML) Antimicrobial peptide Phage therapy Antibiotic stewardship

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Assessing healthcare workers’knowledge and confidence in the diagnosis,management and prevention of Monkeypox

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World Journal of Clinical Cases 2025年第1期13卷 38-47页

作者： Epipode Ntawuyamara Thierry Ingabire Innocent Yandemye Polycarpe Ndayikeza Bina Bhandari Yan-Hua Liang Department of dermatology Cosmetology and VenereologyShenzhen Hospital of Southern Medical UniversityShenzhen 518101Guangdong ProvinceChina Department of dermatology and Venereology Kamenge Teaching HospitalUniversity of BurundiBujumbura 1020Burundi Department of Infectious diseases Kamenge Teaching HospitalUniversity of BurundiBujumbura 1020Burundi General Directorate of Healthcare Supply Modern and Traditional MedicineNutrition and RegistrationMinistry of Public Health and Fight Against HIV/AIDSBujumbura 1055Burundi Department of Epidemiology National Institute of Public HealthBujumbura 350Burundi General Directorate of Planification Ministry of Public Health and Fight Against HIV/AIDSBujumbura 1055Burundi

BACKGROUND Monkeypox(Mpox),is a disease of global public health concern,as it does not affect only countries in western and central *** To assess Burundi healthcare workers(HCWs)s’level of knowledge and confidence in the diagnosis and management of *** We conducted a cross-sectional study via an online survey designed mainly from the World Health Organization course distributed among Burundi HCWs from June-July *** questionnaire comprises 8 socioprofessional-related questions,22 questions about Mpox disease knowledge,and 3 questions to assess confidence in Mpox diagnosis and *** data were analyzed via SPSS software version 25.0.A P value<0.05 was considered to indicate statistical *** The study sample comprised 471 HCWs who were mainly medical doctors(63.9%)and nurses(30.1%).None of the 22 questions concerning Mpox knowledge had at least 50%correct responses.A very low number of HCWs(17.4%)knew that Mpox has a *** confidence level to diagnose(21.20%),treat(18.00%)or prevent(23.30%)Mpox was low among *** confidence level in the diagnosis of Mpox was associated with the HCWs’age(P value=0.009),sex(P value<0.001),work experience(P value=0.002),and residence(P value<0.001).The confidence level to treat Mpox was significantly associated with the HCWs’age(P value=0.050),sex(P value<0.001),education(P value=0.033)and occupation(P value=0.005).The confidence level to prevent Mpox was associated with the HCWs’education(P value<0.001),work experience(P value=0.002),residence(P value<0.001)and type of work institution(P value=0.003).CONCLUSION This study revealed that HCWs have the lowest level of knowledge regarding Mpox and a lack of confidence in the ability to diagnose,treat or prevent *** is an urgent need to organize continuing medical education programs on Mpox epidemiology and preparedness for Burundi *** encourage future researchers to assess potential hesitancy toward Mpox vaccination and its asso

关键词： Monkeypox Public health emergency of international concern Healthcare workers Epidemic Preparedness Knowledge Confidence

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Correlation between diabetic peripheral neuropathy and thyroid hormone sensitivity in elderly patients with type 2 diabetes mellitus

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World Journal of Diabetes 2025年第2期16卷 71-80页

作者： Si-Jia Fei Jing-Yi Luo Wei-Hao Wang Li-Xin Guo Qi Pan Department of Endocrinology Beijing HospitalNational Center of GerontologyInstitute of Geriatric MedicineChinese Academy of Medical SciencesBeijing 100730China

BACKGROUND Diabetic peripheral neuropathy(DPN)is a common complication of type 2 diabetes mellitus(T2DM),significantly affecting patients’quality of life and imposing a substantial economic *** studies have highlighted the role of thyroid hormones in diabetes complications,particularly in elderly patients with ***,the relationship between thyroid hormone sensitivity and DPN remains *** To investigate the correlation between thyroid hormone sensitivity and DPN in elderly patients with *** In a cohort of 256 elderly patients with T2DM,propensity score matching was used to balance age,sex,and diabetes *** data were collected to calculate thyroid hormone sensitivity and analyze its correlation with DPN.A random forest model was used to evaluate the diagnostic value of free triiodothyronine/free thyroxine(FT_(3)/FT_(4))for *** Patients with DPN had a lower FT_(3)/FT_(4) ratio[(0.302±0.053)vs(0.316±0.049),P=0.040].Quartile stratification showed decreasing DPN prevalence with higher FT_(3)/FT_(4) ***’s correlation analysis showed that a lower FT_(3)/FT_(4) ratio was associated with higher glycated hemoglobin,fasting blood glucose,reduced nerve conduction velocity,and electrical skin *** regression indicated a positive relationship between the median FT_(3)/FT_(4) ratio and bilateral foot electrochemical skin conductance[odds ratio(OR):1.019;95%CI:1.005-1.034;P=0.007]and sural nerve sensory amplitude(OR:1.310;95%CI:1.008-1.703;P=0.043).Receiver operating characteristic analysis using a random forest model showed that incorporating FT_(3)/FT_(4) improved predictive performance for DPN,with an area under the curve of 0.74,sensitivity of 0.79,specificity of 0.64,and accuracy of *** In elderly patients with T2DM with euthyroidism,a lower FT_(3)/FT_(4) ratio is correlated with increased DPN incidence,affecting both large and small nerve ***_(3)/FT_(4) is an effective predicto

关键词： Diabetic peripheral neuropathy Thyroid hormone sensitivity Type 2 diabetes mellitus Elderly Free triiodothyronine/free thyroxine ratio

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Effect of cyclophosphamide on fungal infection in SLE mice detected by fluorescent quantitative PCR

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Asian Pacific Journal of Tropical Medicine 2015年第12期8卷 1006-1009页

作者： Hong-Fen Ge Guan-Zhi Chen Min Pan Hui Guo Shandong Provincial hospital for skin diseases Shandong University Shandong Provincial institute of dermatology and Venereology Shandong Academy of Medical Sciences Department of dermatology the Affiliated Hospital of Qingdao University Laboratory Department the Affiliated Hospital of Qingdao University

Objective:To observe the effect of the cyclophosphamide(CTX) on systemic lupus erythematosus mice by fluorescent quantitative polymerase chain ***:(BALB/c C57BL/J6) Fl hybrid mice were used as recipients of donor lymphocytes which were injected *** were randomly divided into control group and treatment group,serum autoantibody and urinary protein were measured,renal pathological changes and the situation of fungal infection were ***:The reduced urinary protein,ameliorated renal lesions were found in the CTX-treated *** albicans and Aspergillus Jlants were detected respectively in respiratory tract and gastrointestinal ***:CTX can alleviate lupus nephritis and the fluorescent quantitative PCR is a rapid and sensitive method for the detection of fungal infection.

关键词： Systemic lupus erythematosus Animal model Fungus P

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