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Helicobacter pylori vacA s1a and s1b alleles from clinical isolates from different regions of Chile show a distinct geographic distribution

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World Journal of Gastroenterology 2005年第40期11卷 6366-6372页

作者： MI Díaz A valdivia P Martínez JL Palacios P Harris J Novales E Garrido D Valderrama C Shilling A Kirberg E Hebel J Fierro R Bravo F Siegel G Leon G Klapp A Venegas departamento de Genética Molecular y Microbiología. Pontificia Universidad Católica de Chile. Santiago departamento de Pediatría Centro de Diagnóstico Terapéutico Los Angeles Unidad de Gastroenterología Anatomía Patológica Centro de Endoscopía Quirúrgica hospital Base de Linares hospital regional de valdivia Instituto de Bioquímica hospital regional de Punta Arenas

AIM: To establish the most common vacA alleles in Helicobacter pylori(H pylori) strains isolated from Chilean patients and its relationship with gastritis and gastroduodenal ulcers.METHODS: Two hundred and forty five H pylori clinical isolates were obtained from 79 biopsies from Chilean infected patients suffering from gastrointestinal diseases. An average of 2-3 strains per patient was isolated and the vacA genotype was analyzed by PCR and 3% agarose electrophoresis. Some genotypes were checked by DNA sequencing.RESULTS: The most prevalent vacA genotype inChilean patients was s1b m1 (76%), followed by s1a m1 (21%). In contrast, the s2 m2 genotype was scarcely represented (3%).The s1b m1 genotype was found most frequently linked to gastropathies (P＜0.05) rather than ulcers. Ulcers were found more commonly in male and older patients. Curiously, patients living in cities located North and far South of Santiago, the capital and largest Chilean city, carried almost exclusively strains with the s1b m1 genotype. In contrast, patients from Santiago and cities located South of Santiago carried strains with either one or both s1a m1 and s1b m1 genotypes.Regarding the s2 m2 genotype, comparison with GenBank sequences revealed that Chilean s2 sequence was identical to those of Australian, American, and Colombian strains but quite different from those of Alaska and India.CONCLUSION: Differences in geographic distribution of the s and m vaccA alleles in Chile and a relationship of s1b m1 genotype with gastritis were found. Sequence data in part support a hispanic origin for the vacA genotype.Asymmetric distribution of genotypes s1b m1 and s2 m2recedes H Pyloristrain distribution in Spain and Portugal.

关键词：地理分布智利幽门螺杆菌单离体

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Expression of HER2 and bradykinin B_1 receptors in precursor lesions of gallbladder carcinoma

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World Journal of Gastroenterology 2012年第11期18卷 1208-1215页

作者： Cesar Toledo Carola E Matus Ximena Barraza Pamela Arroyo Pamela Ehrenfeld Carlos D Figueroa Kanti D Bhoola Maeva del Pozo Maria T Poblete Laboratorio de Patologia Celular Instituto de AnatomiaHistologia y PatologiaUniversidad Austral de ChileValdivia Box 567Chile Servicio de Anatomia Patologica hospital Base Lung Institute of Western AustraliaCentre for Asthma and Allergy Research InstituteUniversity of Western Australia

AIM:To determine the expression of HER2 and bradykinin B 1 receptors(B 1 R) in the two pathogenic models of gallbladder cancer:the metaplasia-dysplasia-carcinoma and the adenoma-carcinoma pathways.METHODS:Receptor proteins were visualized by immunohistochemistry on 5-μm sections of paraffin-embedded tissue.Expression of both receptors was studied in biopsy samples from 92 patients(6 males and 86 females;age ranging from 28 to 86 years,mean 56 years).High HER2 expression in specimens was additionally investigated by fluorescence in situ hybridiza-tion.Cell proliferation in each sample was assessed by using the Ki-67 proliferation marker.RESULTS:HER2 receptor protein was absent in adenomas and in normal gallbladder epithelium.On the contrary,there was intense staining for HER2 on the basolateral membrane of epithelial cells of intestinal metaplasia(22/24;91.7%) and carcinoma in situ(9/10;90%),the lesions that displayed a significantly high proliferation index.Protein up-regulation of HER2 in the epithelium with metaplasia or carcinoma in situ was not accompanied by HER2 gene amplification.A similar result was observed in invasive carcinomas(0/12).The B 1 R distribution pattern mirrored that of HER2 except that B 1 R was additionally observed in the adenomas.The B 1 R appeared either as cytoplasmic dots or labeling on the apical cell membrane of the cells composing the epithelia with intestinal metaplasia(24/24;100%) and carcinoma in situ(10/10;100%) and in the epithelial cells of adenomas.In contrast,both HER2(4/12;33%) and B 1 R(1/12;8.3%) showed a low expression in invasive gallbladder carcinomas.CONCLUSION:The up-regulation of HER2 and B1R in precursor lesions of gallbladder carcinoma suggests cross-talk between these two receptors that may be of importance in the modulation of cell proliferation in gallbladder carcinogenesis.

关键词：受体表达胆囊癌病变肽

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Local renin angiotensin system and sperm DNA fragmentation

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Asian Journal of Andrology 2022年第2期24卷 139-146页

作者： María Victoria Aparicio Prieto María Victoria Rodríguez Gallego Asier valdivia Palacín Yosu Franco Iriarte Gotzone Hervás Barbara Enrique Echevarría Orella Luis Casis Saenz Human Reproduction Unit Cruces University HospitalBarakaldo 48903Spain Human Reproduction Unit San Pedro HospitalLogroño 26006Spain department of Physiology Faculty of Medicine and NursingUniversity of the Basque Country(UPV/EHU)Leioa 48940Spain Human Reproduction Unit Ruber International HospitalMadrid 28034Spain

The renin angiotensin system(RAS)appears to influence male fertility at multiple levels.In this work,we analyzed the relationship between the RAS and DNA integrity.Fifty male volunteers were divided into two groups(25 each):control(DNA fragmentation≤20%)and pathological(DNA fragmentation>20%)cases.Activities of five peptidases controlling RAS were measured fluorometrically:prolyl endopeptidase(which converts angiotensin[A]I and A II to A 1–7),neutral endopeptidase(NEP/CD10:A I to A 1–7),aminopeptidase N(APN/CD13:A III to A IV),aminopeptidase A(A II to A III)and aminopeptidase B(A III to A IV).Angiotensin-converting enzyme(A I to A II),APN/CD13 and NEP/CD10 were also assessed by semiquantitative cytometry and quantitative flow cytometry assays,as were the receptors of all RAS components:A II receptor type 1(AT1R),A II receptor type 2(AT2R),A IV receptor(AT4R or insulin-regulated aminopeptidase[IRAP]),(pro)renin receptor(PRR)and A 1–7 receptor or Mas receptor(MasR)None of the enzymes that regulate levels of RAS components,except for APN/CD13(decrease in fragmented cells),showed significant differences between both groups.Micrographs of RAS receptors revealed no significant differences in immunolabeling patterns between normozoospermic and fragmented cells.Labeling of AT1R(94.3%normozoospermic vs 84.1%fragmented),AT4R(96.2%vs 95.3%)and MasR(97.4%vs 87.2%)was similar between the groups.AT2R(87.4%normozoospermic vs 63.1%fragmented)and PRR(96.4%vs 48.2%)were higher in non-fragmented spermatozoa.These findings suggest that fragmented DNA spermatozoa have a lower capacity to respond to bioactive RAS peptides.

关键词： DNA fragmentation local renin angiotensin system sperm fertility

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Clinical relevance of the use of dentoxol® for oral mucositis induced by radiotherapy: A phase II clinical trial

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World Journal of Clinical Oncology 2022年第10期13卷 813-821页

作者： Sebastián Solé Sergio Becerra Claudia Carvajal Piero Bettolli Hernán Letelier Alejandro Santini Lorena Vargas Alexander Cifuentes Francisco Larsen Natalia Jara Jorge Oyarzún Eva Bustamante Benjamín Martínez David Rosenberg Tomas Galván department of Radiotherapy Radiomedicine InstituteAmérico Vespucio Norte 1314 Vitacura7630370SantiagoChile National Institute of Cancer SantiagoServicio de Salud Metropolitano NorteAv Profesor Zañartu 1010Independencia8380455SantiagoChile Oncologic Institute Arturo López Pérez Foundation JoséManuel Infante 805Providencia7500691SantiagoChile hospital Base valdivia Bueras 1003 s/n XIV Región5100238ValdiviaChile Oncologic Center of Antofagasta Los Pumas 10255Antofagasta1267348AntofagastaChile Universidad Mayor SantiagoAv Libertador Bernardo O´Higgins 20138340585SantiagoChile Ingalfarma Dr Manuel Barros Borgoño 71Oficina 1308Providencia7500593SantiagoChile

BACKGROUND Severe oral mucositis associated with cancer therapy is a frequent complication that may affect a patient's systemic condition,resulting in interruption and/or prolongation of cancer therapy.dentoxol®is a medical solution in the form of a mouthwash that has been shown to result in statistically significant improvement in the prevention of severe oral mucositis.However,knowing the measures of the clinical significance of this therapy is important for accurate decision-making.To describe the clinical impact of dentoxol®use in severe oral mucositis.METHODS Clinical significance was measured using the results obtained in a randomized controlled clinical trial previously conducted by the same group of researchers.The measures of clinical significance evaluated were the absolute risk or incidence,relative risk,absolute risk reduction,relative risk reduction,number needed to treat,and odds ratio.RESULTS The data obtained show that the impact of dentoxol®on reducing the severity of oral mucositis has important clinical relevance.CONCLUSION The results of this study justify the incorporation of dentoxol®mouth rinse into clinical protocols as a complement to cancer therapy to prevent and/or treat oral mucositis secondary to radiotherapy.

关键词： Clinical trial dentoxol Oral mucositis Prevention Radiotherapy Treatment

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Management of Helicobacter pylori infection in Latin America: A delphi technique-based consensus

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World Journal of Gastroenterology 2014年第31期20卷 10969-10983页

作者： Antonio Rollan Juan Pablo Arab M Constanza Camargo Roberto Candia Paul Harris Catterina Ferreccio Charles S Rabkin Juan Cristóbal Gana Pablo Cortés Rolando Herrero Luisa Durán Apolinaria García Claudio Toledo Alberto Espino Nicole Lustig Alberto Sarfatis Catalina Figueroa Javier Torres Arnoldo Riquelme Clínica Alemana de Santiago Facultad de Medicina Clínica Alemana Universidad del Desarrollo departamento de Gastroenterología Facultad de Medicina Pontificia Universidad Católica de Chile Division of Cancer Epidemiology and Genetics National Cancer InstituteRockville MD 20852 United States Programa de Salud Basada en Evidencia Pontificia Universidad Católica de Chile departamento de Gastroenterología y Nutrición Pediátrica Facultad de Medicina Pontificia Universidad Católica de Chile departamento de Salud Pública Facultad de Medicina Pontificia Universidad Católica de Chile International Agency for Research on Cancer World Health Organization departamento de Microbiología Facultad de Ciencias Biológicas Universidad de Concepción Unidad de Gastroenterología Hospital de Valdivia. Facultad de Medicina Universidad de Austral de Chile Unidad de Investigación en Enfermedades Infecciosas Instituto Mexicano del Seguro Social

AIM: To optimize diagnosis and treatment guidelines for this geographic region, a panel of gastroenterologists, epidemiologists, and basic scientists carried out a structured evaluation of available literature.METHODS: Relevant questions were distributed among the experts, who generated draft statements for consideration by the entire panel. A modified three-round delphi technique method was used to reach consensus. Critical input was also obtained from representatives of the concerned medical community. The quality of the evidence and level of recommendation supporting each statement was graded according to United States Preventive Services Task Force criteria.RESULTS: A group of ten experts was established. The survey included 15 open-ended questions that were distributed among the experts, who assessed the articles associated with each question. The levels of agreement achieved by the panel were 50% in the first round, 73.3% in the second round and 100% in the third round. Main consensus recommendations included:(1) when available, urea breath and stool antigen test(HpSA) should be used for non-invasive diagnosis;(2) detect and eradicate Helicobacter pylori(H. pylori) in all gastroscopy patients to decrease risk of peptic ulcer disease, prevent o retard progression in patients with preneoplastic lesions, and to prevent recurrence in patients treated for gastric cancer;(3) further investigate implementation issues and health outcomes of H. pylorieradication for primary prevention of gastric cancer in high-risk populations;(4) prescribe standard 14-d triple therapy or sequential therapy for first-line treatment;(5) routinely assess eradication success post-treatment in clinical settings; and(6) select second- and third-line therapies according to antibiotic susceptibility testing.CONCLUSION: These achievable steps toward better region-specific management can be expected to improve clinical health outcomes.

关键词： Helicobacter pylori Consensus development conferen

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Transvaginal Approach for Nongynecologic Intraperitoneal Procedures

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International Journal of Clinical Medicine 2014年第21期5卷 1417-1429页

作者： Jose F.Noguera Gonzalo Martin Jose M.Munoz Antonio Melero Raul Sanchez Javier valdivia Marcos Bruna Antonio Salvador Cristobal Zaragoza Minimally Invasive Therapies Unit Consorcio Hospital General Universitario de ValenciaValenciaSpain department of Surgery Hospital Son LlatzerPalmaSpain department of Surgery Consorcio Hospital General Universitario de ValenciaValenciaSpain department of Surgery Hospital Virgen de las NievesGranadaSpain

The minimally invasive surgery through natural orifices has revolutionized the laparoscopic surgery for abdominal procedures. The use of the vaginal approach is not new for gynecologists but it is a new concept for the non-gynecological laparoscopic surgeons. The use of this new approach has been used to perform some procedures and to extract specimens after a laparoscopic surgery, but we don’t know exactly the number of procedures performed until today. There are few papers with clinical experience and a lot of philosophical papers about NOTES. Our aim is to know how and how often we are using this new approach for non-gynecological abdominal minimally invasive procedures. With the revision of the PubMed publications we obtained a total of 268 articles, of which 125 were included in the analysis (46.64%). Cholecystectomy was the procedure more usually performed: there is a large clinical experience with a total of 2432 transvaginal cholecystectomies. Bariatric surgery, colectomy and appendectomy have been other surgical procedures with some clinical experience in the use of the transvaginal approach. Analyzing the publications on transvaginal approach, we observed that the use of the vaginal route for non-gynecological abdominal surgery was not anecdotal, with accumulative experience of more than 3000 patients reported in published studies. The use of the vaginal route has shown its safety, obtaining some additional benefits such as the aesthetic and the faster functional recovery. Dyspareunia, one of the most feared, has not been reported in studies as a concern to consider.

关键词： Transvaginal Surgery Endoscopic Surgery Laparoscopy Flexible Endoscopy

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Eustachian Valve Endocarditis—First <i>Staphylococcus epidermidis</i>Case description

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World Journal of Cardiovascular Surgery 2013年第5期3卷 143-145页

作者： Emiliano Andrés Rodríguez-Caulo José María Novoa-Medina Diego valdivia-Miranda Javier Pérez-López Cardiology department Hospital Universitario de Gran Canaria Doctor Negrín Las Palmas de Gran Canaria Spain Cardiovascular Surgery department Hospital Universitario de Gran Canaria Doctor Negrín Las Palmas de Gran Canaria Spain

Eustachian Valve Endocarditis (EVE) has been considered an extremely rare clinical entity and only 26 adult cases have been reported in literature. Eustachian valve (EV) is an embryological remnant of the sinus venosus, redirecting oxy-genated fetal blood from inferior vena cava across foramen ovale and left atrium. In adults it is considered a benign rudimentary structure and its persistence is uncommon. Only 4% of the population presents a visible and redundant EV by echocardiography. Up to 10% of infective endocarditis (IE) are right sided and occurs predominantly in patients with predisposing factors as intravenous drug abusers, permanent pacemaker wires, central venous lines, or immunologic disorders, predominantly in tricuspid valve, but with a reported incidence up to 3.3% of EVE. Vast majority of EVE are due to S. aureus in 53%-90% cases. We strongly recommend analysing the EV when echocardiography is performed in a patient suspected of having IE, irrespective of whether vegetation in another valve is found. In this case report we describe the first EVE related to Staphylococcus epidermidis in a 35-year-old male with permanent Shaldon-catheter.

关键词： Endocarditis Valves Echocardiography

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Laparoscopic ureterolysis with simultaneous ureteroscopy and percutaneous nephroscopy for treating complex ureteral obstruction after failed endoscopic intervention:A technical report

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Asian Journal of Urology 2015年第4期2卷 238-243页

作者： Zhixiang Wang Bing Liu Xiaofeng Gao Yi Bao Yang Wang Huamao Ye Yinghao Sun Linhui Wang department of Urology Changzheng HospitalSecond Military Medical UniversityShanghaiChina department of Urology Changhai HospitalSecond Military Medical UniversityShanghaiChina

Objective:Complex ureteral obstruction is refractory to conventional urological intervention.This report describes a case of laparoscopic ureterolysis with simultaneous ureteroscopy and percutaneous nephroscopy for treating complex ureteral obstruction.Methods:Right-side multiple ureteral stones and complicating ureteral obstruction failed an initial attempt of ureteroscopy lithotripsy with simultaneous percutaneous nephroscopy in a 23-year-old male.Laparoscopic ureterolysis with ureteroscopy and percutaneous nephroscopy was used simultaneously to dissect the periureteral adhesions with the patient placed in the Galdakao-modified supine valdivia position.The ureter was incised to allow the insertion of a ureteral catheter through the twisted ureter,and a guide wire was advanced into the pelvis using ureteroscopy.A double-J stent was placed into the right-side ureter using antegrade percutaneous nephroscopy.Results:The laparoendoscopic procedure lasted 330 min with an estimated bleeding volume of 100 mL.The patient underwent an uneventful postoperative course,and postoperative followup radiography confirmed good positioning of the double-J stent.The double-J stent was removed 3 months after operation.The patient remained asymptomatic within a 13-month follow-up period.Conclusion:Laparoscopic ureterolysis with simultaneous ureteroscopy and percutaneous nephroscopy is an effective and safe treatment option for complex ureteral obstruction.

关键词： Ureteral obstruction Laparoscopic ureterolysis Ureteroscopy Percutaneous nephrostomy

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DLST mutations in pheochromocytoma and paraganglioma cause proteome hyposuccinylation andmetabolic remodeling

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Cancer Communications 2023年第7期43卷 838-843页

作者： Sara Mellid Fernando García Luis Javier Leandro-García Alberto Díaz-Talavera Ángel Mario Martínez-Montes Eduardo Gil Bruna Calsina María Monteagudo Rocío Letón Juan María Roldán-Romero María Santos Javier Lanillos Carlos valdivia Natalia Martínez-Puente Javier de Nicolás-Hernández Scherezade Jiménez Manuel Pérez-Martínez Emiliano Honrado Javier Coloma Ana Cerezo Clara María Santiveri Manel Esteller Ramón Campos-Olivas Eduardo Caleiras Cristina Montero-Conde Cristina Rodríguez-Antona Javier Muñoz Mercedes Robledo Alberto Cascón Hereditary Endocrine Cancer Group Spanish National Cancer Research Centre(CNIO)MadridSpain Proteomics Unit Spanish National Cancer Research Centre(CNIO)MadridSpain Centro de Investigación Biomédica en Red de Enfermedades Raras(CIBERER) MadridSpain Monoclonal Antibodies Core Unit Spanish National Cancer Research Centre(CNIO)MadridSpain Confocal Microscopy Core Unit Spanish National Cancer Research Centre(CNIO)MadridSpain Anatomical Pathology Service Hospital of LeónLeónCastilla y LeónSpain Structural Biology Programme Spanish National Cancer Research Centre(CNIO)MadridSpain CNIO-Lilly Cell Signalling and Immunometabolism Section Spanish National Cancer Research Centre(CNIO)MadridSpain Spectroscopy and Nuclear Magnetic Resonance Unit Spanish National Cancer Research Centre(CNIO)MadridSpain Josep Carreras Leukaemia Research Institute(IJC) BadalonaBarcelonaSpain Centro de Investigacion Biomedica en Red Cancer(CIBERONC) MadridSpain Institucio Catalana de Recerca i Estudis Avançats(ICREA) BarcelonaSpain Physiological Sciences department School of Medicine and Health SciencesUniversity of Barcelona(UB)L’HospitaletBarcelonaSpain Histopathology Core Unit Spanish National Cancer Research Centre(CNIO)MadridSpain Cell Signaling and Clinical Proteomics Group Biocruces Bizkaia Health Research InstituteBarakaldoSpain Ikerbasque Basque Foundation for ScienceBilbaoSpain

dear Editor,Of all human tumors,pheochromocytomas and paragangliomas(PPGLs)have the highest heritability rate.Over 15%of PPGLs harbor mutations in genes encoding tricarboxylic acid(TCA)cycle-related enzymes that cause oncometabolite accumulation and drive tumorigenesis via metabolic adaptation to hypoxia and global hypermethylation[1].The dihydrolipoamide S-succinyltransferase(DLST)gene was recently described as a new PPGL susceptibility gene[2].

关键词： glioma remodeling cytoma

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Treatment strategies in cervical cancer:treatment of advanced disease

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Journal of Cancer Metastasis and Treatment 2022年第1期8卷 221-239页

作者： Augusto valdivia Juan Francisco Grau-Béjar Carmen García-Durán Ana Oaknin Gynaecologic Cancer Programme Vall d’Hebron Institute of Oncology(VHIO) Hospital Universitari Vall d’HebronVall d’Hebron Barcelona Hospital CampusBarcelona 08035Spain

Cervical cancer is the fourth most common cancer in women worldwide,with a global incidence of 604,127 and an annual death rate of 341,831 in 2020.Patients with recurrent,persistent,or metastatic disease not amenable to curative therapy represent a patient population with a dismal prognosis.Until recently,the standard of care for these patients was based on platinum doublet chemotherapy with or without bevacizumab.However,significant advances in the treatment landscape of this disease have recently been achieved with the incorporation,among others,of immunotherapy in the therapeutic armamentarium.This review summarizes the main treatment approaches developed throughout the past decades,with particular emphasis on immunotherapy and novel targeted therapies.

关键词： Cervical cancer advanced stage chemotherapy immunotherapy antiangiogenic agents targeted therapy

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