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Advances in Bioscience and Biotechnology 2018年第5期9卷 205-214页

作者： Zuraida Ab Rahman Shazwan Abd Shukor Hartinee Abbas Chandradevan A. L. Machap Mohd Suhaimi Bin Alias Razali Mirad Syairah Sofiyanand Ayu Nazreena Othman Biotechnology & amp Nanotechnology research centre MARDI HQ Persiaran MARDI-UPM Serdang Malaysia horticulture research centre mardi sintok Bukit Kayu Hitam Malaysia Food Technology research centre MARDI HQ Persiaran MARDI-UPM Serdang Malaysia Agrobiodiversity and Environment research centre MARDI HQ Persiaran MARDI-UPM Serdang Malaysia Nilai Polytechnic Kompleks Pendidikan Bandar Enstek Bandar Enstek Malaysia

Kaempferia parviflora plants derived from in vitro culture were grown in the glasshouse. A comparison of the yield of total phenolics and total flavonoids under varying extraction conditions from rhizomes harvested from plants of different ages was undertaken. The results showed that phenolic and flavonoid contents in the rhizomes were highest 8 months after planting. Another study found that 2 g rhizomes extracted in 50 ml of water at 90°C for 120 minutes gave the best yield of phenolics and flavonoids. Under these conditions, an average of 210 mg GAE/g dry weight of total phenolics and 81 μg QCE/g dry weight of total flavonoids were obtained.

关键词： Phenolics Flavonoids Kaempferia parviflora Extraction

Effects of cucumber mosaic virus-infected chilli plants on non-vector Bemisia tabaci (Hemiptera Aleyrodidae)

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Insect Science 2019年第1期26卷 76-85页

作者： Khalid A.Saad M.N.Mohamad Roff Rebecca H.Hallett Idris B.Abd-Ghani Faculty of Science and Technology School of Environmental and Natural Resource SciencesNational University MalaysiaBangi SelangorMalaysia horticulture research centre HeadquartersMARDI-UPMSerdang SelangorMalaysia School of Environmental Sciences University of GuelphGuelphOntarioCanada

Plant virus infections are known to alter host plant attractiveness and suitability for insect *** study was conducted to determine how cucumber mosaic virus (CMV)-infected chilli plants affect the fitness and settling preferences ofnonvector whitefly,Bemisia tabaci adults under dual-choice conditions with volatile organic compounds analyzed using solid phase microextraction coupled with gas chromatography-mass spectrometry (GC-MS).Results showed that the presence of CIVIV in chilli plants substantially affects the settling preferences of the ***,which preferred to settle on noninfected *** of the egg stage and the longevity and fecundity of adult *** on CMV-infected chilli plants were not markedly different from those on noninfected chilli *** contrast,the developmental time from egg to adult was significantly reduced in CMV-infected chilli plants compared to the noninfected *** results also showed that CMV-infected chilli plants released significantly more linalool and phenylacetaldehyde than noninfected ***,it was suggested that the behavioral response of *** might be modified by CMV-infected plants,which alter the release of specific headspace *** on these results,the modification of plant volatile profiles may help in enhancing the effectiveness of biological control and the protection of crop plants against ***.

关键词： Bromoviridae Capsicum anmium cucumovirus in sect-pathogen interactions whitefly

Influence of Agitation Rate on the Growth of MD2 Pineapple Protocorm-Like Bodies and Shoots in Liquid-Shake Culture

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American Journal of Plant Sciences 2019年第7期10卷 1233-1238页

作者： Zuraida Ab Rahman Hartinee Abbas Ayu Nazreena Othman Wan Zaliha Wan Sembok Biotechnology and Nanotechnology research centre Malaysian Agricultural Research and Development Institute (MARDI) Kuala Lumpur Malaysia horticulture research centre Malaysian Agricultural Research and Development Institute (MARDI) Sintok Malaysia School of Food Science and Technology Universiti Malaysia Terengganu Kuala Terengganu Malaysia

The present study was conducted to investigate the effect of agitation rate on the increase in fresh weight of MD2 pineapple protocorm-like bodies (PLBs) and shoots cultured in liquid medium. PLBs were cultured in 250 ml Erlenmeyer flasks (7 g per flask) containing MS medium and plant growth regulators (1.5 mg/L 6-Benzylaminopurine, BAP and 0.2 mg/L 1-Naphthaleneacetic acid, NAA). The orbital shaker was set at speeds of 50, 80, 100, 120, and 150 rpm. After 40 days, the cultures shaken at 80 rpm showed the highest fresh weight and the highest number of shoots at 76 g and 41 shoots, respectively. A comparative study of agitation found that 80 rpm was the best speed which enhanced both PLB and shoot formation. The findings in the present study would be helpful in setting up large-scale in vitro mass propagation of MD2 pineapple.

关键词： Pineapple MD2 Micropropagation Liquid-Shake Culture Agitation Rate

Discovery of Simple Sequence Repeat Markers through Transcriptome Analysis of Baccaurea motleyana

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Journal of Food Science and Engineering 2020年第2期10卷 33-46页

作者： Khairun Hisam Nasir Muhammad Fairuz Mohd Yusof Mohd Shahril Firdaus Abdul Razak Siti NorsaidahIbrahim Mira Farzana Mohamad Moktar Khadijah Awang Mohd Shukri Mat Ali centre for Molecular Marker Discovery and Validation(CMDV) Agri-Omic dan BioinformaticsBiotechnology and Nanotechnology Research CentreMalaysian Agricultural Research and Development Institute(MARDI)Serdang 43400SelangorMalaysia Industrial Crop research centre Malaysian Agricultural Research and Development Institute(MARDI)Serdang 43400SelangorMalaysia horticulture research centre Malaysian Agricultural Research and Development Institute(MARDI)Serdang 43400SelangorMalaysia

Baccaurea motleyana(rambai)is underutilized fruits that are native to Malaysia,Indonesia and *** this study,a total of 54,779 unigenes identified from rambai transcriptome were used for simple sequence repeat(SSR)analysis by MIcroSAtellite(MISA).A total of 20,420 SSRs were found to be distributed within 37.27%of the total number of *** repeats represented the main type,accounting for 64.04%,followed by trinucleotide repeats(20.28%)and dinucleotide repeats(19.94%).Gene annotation to seven databases has a success ratio of 68.53%(National Center for Biotechnology Information(NCBI)protein sequences),53.68%(NCBI nucleotide sequences),27.43%(Kyoto Encyclopedia of Genes and Genome Ortholog),56.0%(SwissProt),52.44%(Protein family),53.99%(Gene Ontology)and 26.44%(Kluster of Orthologous Group).Further rambai SSRs were randomly selected and validated to ***(rambai),***(tampoi),***(jentik-jentik),***(pupor)and ***(setambun).

关键词： Baccaurea SSR transcriptome gene annotation and SSR validation

Development of Starfruit Simple Sequence Repeat (SSR) Using Next Generation Sequencing

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Journal of Food Science and Engineering 2019年第3期9卷 95-121页

作者： Khairun Hisam Nasir Muhammad Fairuz Mohd Yusof Mohd Shahril Firdaus Siti Zainab Jantan Mira Farzana Mohamad Moktar Siti Norsaidah Ibrahim Noor Baiti Abdul Aziz Joanna Cho Lee Ying centre for Molecular Marker Discovery and Validation (CMDV) Agri-Omic dan Bioinformatics Biotechnology and Nanotechnology Research Centre Malaysian Agricultural Research and Development Institute (MARDI) 43400 Serdang Selangor Malaysia horticulture research centre Headquarters Malaysian Agricultural Research and Development Institute (MARDI) 43400 Serdang Selangor Malaysia

Starfruit (Averrhoa carambola L.) is an important fruit for Malaysian export and great attention has been made to improve starfruit fruit quality at Malaysian Agricultural research and Development Institute (mardi). The current study used next generation sequencing (NGS) technologies to develop starfruit simple sequence repeat (SSR) from 2 varieties namely B11 and B17 using Illumina HiSeq. The pre-processed reads were de novo assembled to generate approximately 75,000 and 74,000 scaffolds respectively. Total genome size for B11 and B17 were around 345 Mbp and 342 Mbp based on K-mer distribution analysis. In-silico microsatellite mining of each variety has identified more than 17,000 SSR in B11 and B17 respectively. Dinucleotides were the most abundant, accounting for more than 70% of all SSR and repeat motif GA (49%) was most common. A total of 239 SSR primer pairs were designed from contigs larger than 350 nucleotides and tested for amplification. The 30 polymorphic SSRs were used to DNA fingerprint of 12 starfruit hybrids. Polymorphism information content (PIC) ranged from 0.1411 to 0.6838, with an average of 0.3919. The Unweighted Pair-Group Method for Arithmetic Averages (UPGMA) dendrogram clustered 12 starfruit accessions into 2 groups.

关键词： Starfruit (Averrhoa carambola L.) next generation sequencing molecular marker simple sequence repeat