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Exploring the characteristics of microbial community and enzyme activity in the aged refuse landfill environment of Taiyuan, China

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Pedosphere 2024年第5期34卷 879-891页

作者： Fen HOU Junjie DU Yuzhou MENG Xihui WU Department of Land resource Management School of Public Administration Shanxi University of Finance and Economics College of Life Science Shanxi Engineering Research Center of Microbial Application Technologies Shanxi Normal University

Municipal solid waste landfill is the main disposal option for domestic garbage, in which microbial activities play an important role. However, despite the widespread practice of landfilling, the metagenomic microbial profiles of landfill sites remain poorly characterized. In this study, we used a combination of physicochemical analysis, ultraviolet-visible spectrophotometry, and high-throughput Illumina shotgun sequencing to systematically investigate the changes in soil enzyme activities, microbial community structure, and functional attributes in aged refuse collected from the Xingou Municipal Solid Waste Landfill in Taiyuan, China, with ordinary topsoil from an area within 5 km of the landfill as control soil. Except for neutral phosphatase (P=0.065), the activities of urease, laccase, dehydrogenase, sucrase, neutral protease, and β-glucosidase were all significantly reduced (P <0.05) in the aged refuse compared with the control soil. Contrastingly, catalase activity was found to be significantly elevated in the aged refuse. Compared with the control soil, aged refuse was characterized by higher richness and diversity of microbial communities, as reflected by the higher values of community richness estimators (Chao 1 and ACE) and diversity indices (Shannon and Simpson). In total, 186 phyla, 4 354 genera, and 34 459 species were identified, with 132 phyla, 1 914 genera, and7 369 species showing significantly different abundances between the aged refuse and the control soil. Actinobacteria and Acidobacteria were identified as the dominant phyla in the control soil, whereas Proteobacteria, Euryarchaeota (archaea), and Firmicutes were found to predominate in the aged ***, Euryarchaeota and Methanoculleus were the major taxa detected in the aged refuse, but were almost completely absent in the control soil. Xenobiotic biodegradation and bacterial chemotaxis were the main functions of the microflora in the aged refuse, whereas the carbohydrate, amino acid,

关键词： functional attribute metabolism pathway municipal solid waste landfill resource utilization soil microflora

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Cooperative effect of sodium lauryl sulfate collector and sodium pyrophosphate depressant on the flotation separation of lead oxide minerals from hematite

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International Journal of Minerals,Metallurgy and Materials 2024年第9期31卷 1975-1984页

作者： Honghu Tang Bingjian Liu Mengshan Li Qiancheng Zhang Xiongxing Zhang Feng Jiang School of Minerals Processing and Bioengineering Central South UniversityChangsha 410083China Engineering Research center of Ministry of Education for Carbon Emission Reduction in Metal resource Exploitation and Utilization Central South UniversityChangsha 410083China

As a cornerstone of the national economy,the iron and steel industry generates a significant amount of sintering dust containing both valuable lead resources and deleterious *** is a promising technique for lead recovery from sintering dust,but efficient separation from Fe_(2)O_(3) is still *** study investigated the cooperative effect of sodium lauryl sulfate(SLS,C_(12)H_(25)SO_(4)Na)and sodium pyrophosphate(SPP,Na_(4)P_(2)O_(7))on the selective flotation of lead oxide minerals(PbOHCl and PbSO_(4))from hematite(Fe_(2)O_(3)).Optimal flotation conditions were first identified,resulting in high recovery of lead oxide minerals while inhibiting Fe_(2)O_(3) *** potential measurements,Fourier transform infrared spectroscopy(FT-IR)analysis,adsorption capacity analysis,and X-ray photoelectron spectroscopy(XPS)studies offer insights into the adsorption behaviors of the reagents on mineral surfaces,revealing strong adsorption of SLS on PbOHCl and PbSO_(4) surfaces and remarkable adsorption of SPP on Fe_(2)O_(3).The proposed model of reagent adsorption on mineral surfaces illustrates the selective adsorption behavior,highlighting the pivotal role of reagent adsorption in the separation *** findings contribute to the efficient and environmentally friendly utilization of iron ore sintering dust for lead recovery,paving the way for sustainable resource management in the iron and steel industry.

关键词： sintering dust flotation separation sodium lauryl sulfate sodium pyrophosphate selective adsorption

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Numerical and theoretical study of load transfer behavior during cascading pillar failure

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第8期16卷 3014-3033页

作者： Hangyu Dong Wancheng Zhu Leilei Niu Chen Hou Xige Liu College of Geosciences and Engineering North China University of Water Resources and Electric PowerZhengzhou450046China center for Rock Instability and Seismicity Research School of Resource and Civil EngineeringNortheastern UniversityShenyang110819China

To further study the load transfer mechanism of roofemulti-pillarefloor system during cascading pillar failure(CPF),numerical simulation and theoretical analysis were carried out to study the three CPF modes according to the previous experimental study on treble-pillar specimens,*** failure mode(SFM),domino failure mode(DFM)and compound failure mode(CFM).Based on the finite element code rock failure process analysis(RFPA^(2D)),numerical models of treble-pillar specimen with different mechanical properties were established to reproduce and verify the experimental results of the three CPF *** results show that the elastic rebound of roofefloor system induced by pillar instability causes dynamic disturbance to adjacent pillars,resulting in sudden load increases and sudden jump displacement of adjacent *** phenomena of load transfer in the roofemulti-pillarefloor system,as well as the induced accelerated damage behavior in adjacent pillars,were discovered and *** addition,based on the catastrophe theory and the proposed mechanical model of treble-pillar specimen edisc spring group system,a potential function that characterizes the evolution characteristics of roof emulti-pillarefloor system was *** analytical expressions of sudden jump and energy release of treble-pillar specimenedisc spring group system of the three CPF modes were derived according to the potential *** numerical and theoretical results show good agreement with the experimental *** study further reveals the physical essence of load transfer during CPF of roof emulti-pillarefloor system,which provides references for mine design,construction and disaster prevention.

关键词： Cascading pillar failure(CPF) Load transfer Multi-pillar Numerical simulation

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Unveiling the performance of a novel alkalizing bacterium Enterobacter ***-2 in immobilization of available Cd

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Journal of Environmental Sciences 2024年第3期137卷 245-257页

作者： Yixin Luo Min Liao Xiongxiong Lu Na Xu Xiaomei Xie Weiming Gao College of Environmental and resource Science Zhejiang UniversityHangzhou 310058China Zhejiang Provincial Key Laboratory of Agricultural resources and Environment Hangzhou 310058China National Demonstration center for Experimental Environmental and resources Education Zhejiang UniversityHangzhou 310058China

A novel alkalizing strain Enterobacter ***-2 that could resist 400 mg/L Cd was isolated from Cd-contaminated soil,which immobilized 96.05%Cd^(2+)from *** distribution analysis demonstrated that more than half of the Cd^(2+)was converted into extracellular precipitated Cd through mobilization of the alkali-producing mechanism by the strain LYX-2,achieving the high immobilization efficiency of Cd^(2+).Biosorption experiments revealed that strain LYX-2 had superior biosorption capacity of 48.28 mg/g for *** experiments with Brassica rapa *** performed with and without strain *** to control,15.92%bioavailable Cd was converted to non-bioavailable Cd and Cd content in aboveground vegetables was decreased by 37.10%with addition of strain *** Cd was mainly immobilized through extracellular precipitation,cell-surface biosorption and intracellular accumulation of strain LYX-2,which was investigated through Cd distribution,Scanning Electron Microscope and Energy-Dispersive X-ray Spectroscopy(SEM-EDS),Fourier Transform Infrared Spectroscopy(FT-IR),X-ray Photoelectron Spectroscopy(XPS)and Transmission Electron Microscopy(TEM)*** addition,the application of strain LYX-2 significantly promoted the growth of vegetables about *** results indicated that highly Cd-resistant alkalizing strain LYX-2,as a novel microbial passivator,had excellent ability and reuse value to achieve the remediation of Cd-contaminated soil coupled with safe production of vegetables simultaneously.

关键词： Cadmium Enterobacter sp.LYX-2 Cd Immobilization Microbial passivator

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Extract lithium from clay-type lithium ore by mixed acid and its mechanism Author links open overlay panel

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Particuology 2024年第8期91卷 323-332页

作者： Wuchang Zhou Ruiqi Xie Xiong Tong Xian Xie Yang Liu Zhihui Zhao Faculty of Land resource Engineering Kunming University of Science and TechnologyKunming650093China Technology Innovation center for Comprehensive Utilization of Strategic Mineral resources Ministry of Natural ResourcesChengdu610041China National&Regional Engineering Research center for Green comprehensive Utilization of Tailing resources from Metal Minerals Kunming650093China

A roasting-leaching test was carried out for the efficient utilization of clay-type lithium ore in the central region of Yunnan *** test used the mixed acid of sulfuric acid and phosphoric acid as the leaching *** the conditions of roasting temperature of 600℃,roasting time of 1 h,liquid-solid ratio of 5:1,volume ratio of H_(2)SO_(4)solution to H_(3)PO_(4)solution of 45:5,leaching time of 2 h and leaching temperature of 80℃,the leaching rate of lithium was as high as 97.83%.The leaching mechanism was studied by SEM,pore property analysis,XRD and *** was found that the morphology of the ore changed obviously after roasting and leaching,and a certain degree of collapse and fragmentation occurred,which provided favorable spatial conditions for the leaching of *** porosity,total intrusion volume and total pore area also increased after roasting and leaching,thus promoting the leaching of Li^(+).The results showed that chemical reaction taken placed during the roasting and *** phase of the sample changed from chlorite,kaolinite and diaspore(boehmite)mainly to corundum,hematite,periclase and quartz after ***,after leaching,no new phase was produced in the ore sample,and no S and P elements were found on the surface of the ore sample,indicating that the leaching mechanism of lithium might be the ion exchange between H^(+)and Li^(+).

关键词： Clay-type lithium ore Lithium extraction Roasting Leaching

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Dermal fibroblast expression of stromal cellderived factor-1 （SDF-1） promotes epidermal keratinocyte proliferation in normal and diseased skin

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Protein & Cell 2015年第12期6卷 890-903页

作者： Chunji Quan Moon Kvun Cho Yuan Shao Laurel E. Mianecki Eric Liao Daniel Perry Taihao Quan Department of Pathology Affiliated Hospital of Yanbian University Yanji 133000 China Department of Dermatology Soonchunhyang University College of Medicine Seoul Republic of Korea hollings cancer center biorepository and tissue analysis resource Medical University of South Carolina Charleston SC 29425 USA Department of Dermatology University of Michigan Medical School Ann Arbor MI 48109 USA

Stromal cells provide a crucial microenvironment for overlying epithelium. Here we investigated the expression and function of a stromal cell-specific protein, stromal cell-derived factor-1 （SDF-1）, in normal human skin and in the tissues of diseased skin. Immunohistology and laser capture microdissection （LCM）-coupled quantitative real- time RT-PCR revealed that SDF-1 is constitutively and predominantly expressed in dermal stromal cells in nor- mal human skin in vivo. To our surprise, an extremely high level of SDF-1 transcription was observed in the dermis of normal human skin in vivo, evidenced by much higher mRNA expression level than type I collagen, the most abundant and highly expressed protein in human skin. SDF-1 was also upregulated in the tissues of many human skin disorders including psoriasis, basal cell carcinoma （BCC）, and squamous cell carcinoma （SCC）. Double immunostaining for SDF-1 and HSP47 （heat shock protein 47）, a marker of fibroblasts, revealed that fibroblasts were the major source of stroma-cell-derived SDF-1 in both normal and diseased skin. Functionally, SDF-1 activates the ERK （extracellular-signal-regulated kinases） pathway and functions as a mitogen to stimulate epidermalkeratinocyte proliferation. Both overexpression of SDF-1 in dermal fibroblasts and treatment with rhSDF-1 to the skin equivalent cultures significantly increased the number of keratinocyte layers and epidermal thickness. Con- versely, the stimulative function of SDF-1 on keratinocyte proliferation was nearly completely eliminated by inter- fering with CXCR4, a specific receptor of SDF-1, or by knock-down of SDF-1 in fibroblasts. Our data reveal that extremely high levels of SDF-1 provide a crucial microenvironment for epidermal keratinocyte proliferation in both physiologic and pathologic skin conditions.

关键词： SDF-1 dermal fibroblast keratinocyte,proliferation skin cancer psoriasis

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Sliding behaviors of the trapezoidal roof rock block under a lateral dynamic disturbance

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第3期16卷 741-760页

作者： Feng Dai Wancheng Zhu Min Ren Shunchuan Wu Leilei Niu Faculty of Land resources Engineering Kunming University of Science and TechnologyKunming650093China center for Rock Instability and Seismicity Research Department of Mining EngineeringSchool of Resource and Civil EngineeringNortheastern UniversityShenyang110819China

The surrounding rock of underground space is always affected by external dynamic disturbance from the side position,such as blasting vibration from a stope at the same level or seismic waves from adjacent strata.A series of laboratory tests,numerical simulations and theoretical analyses were carried out in this study to disclose the sliding mechanism of roof rock blocks under lateral ***,the experiments on trapezoidal key block under various clamping loads and disturbance were conducted,followed by numerical simulations using the fast Lagrangian analysis of continua(FLAC3D).Then,based on the conventional wave propagation model and the classical shear-slip constitutive model,a theoretical model was proposed to capture the relative displacement between blocks and the sliding displacement of the key *** results indicate that the sliding displacement of the key block increased linearly with the disturbance energy and decreased exponentially with the clamping load when the key block was disturbed to slide(without instability).Meanwhile,when the key block was disturbed to fall,two types of instability process may appear as immediate type or delayed *** addition,the propagation of stress waves in the block system exhibited obvious low-velocity and lowfrequency characteristics,resulting in the friction reduction effect appearing at the contact interface,which is the essential reason for the sliding of rock *** results can be applied to practical underground engineering and provide valuable guidance for the early detection and prevention of rockfalling disasters.

关键词： Lateral dynamic disturbance Trapezoidal rock block system Low-velocity and low-frequency wave Friction reduction effect Instability mode

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Quantifying China's iron and steel industry's CO2 emissions and environmental health burdens:A pathway to sustainable transformation

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Environmental Science and Ecotechnology 2024年第4期20卷 189-197页

作者： Weiling Wu Qian Tang Wenbo Xue Xurong Shi Dadi Zhao Zeyuan Liu Xin Liu Chunlai Jiang Gang Yan Jinnan Wang State Environmental Protection Key Laboratory of Environmental Pollution and Greenhouse Gases Co-control Chinese Academy of Environmental PlanningBeijing100041China center of Air Quality Simulation and System analysis Chinese Academy of Environmental PlanningBeijing100041China State Environmental Protection Key Laboratory of Environmental Planning and Policy Simulation Chinese Academy of Environmental PlanningBeijing100041China College of Environmental&resource Sciences Zhejiang UniversityHangzhou310058China

Assessing the iron and steel industry's(ISI)impact on climate change and environmental health is vital,particularly in China,where this sector significantly influences air quality and CO_(2)*** is a lack of comprehensive analyses that consider the environmental and health burdens of manufacturing processes for ISI ***,we present an integrated emission inventory that encompasses air pollutants and CO_(2)emissions from 811 ISI enterprises and five key manufacturing processes in *** analysis shows that sintering is the primary source of air pollution in the *** contributes 71%of SO_(2),73%of NO_(x),and 54%of PM_(2.5)*** the other hand,81%of total CO_(2)emissions come from blast ***,the contributions of ISI have resulted in an increase of 3.6 mg m^(-3)in national population-weighted PM_(2.5)concentration,causing approximately 59,035 premature deaths in *** from Hebei,Jiangsu,Shandong,Shanxi,and Inner Mongolia provinces contributed to 48%of PM_(2.5)-related deaths in ***,the transportation of air pollutants across provincial borders highlights a concerning trend of environmental health *** on the research findings,it is crucial for ISI manufacturers to prioritize the removal of outdated production capacities and adopt energy-efficient and advanced techniques,along with ultra-low emission *** is particularly important for those manufacturers with substantial environmental *** transformative actions are essential in mitigating the environmental and health impacts in the affected regions.

关键词： Iron and steel industry CO_(2)emissions Air pollutants emissions Environmental health

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Scientific and technological innovations of wastewater treatment in China

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Frontiers of Environmental Science & Engineering 2024年第6期18卷 43-48页

作者： Hui Huang Rui Ma Hongqiang Ren State Key Laboratory of Pollution Control and resource Reuse School of the EnvironmentNanjing UniversityNanjing 210023China

The“dual-carbon”strategy promotes the development of the wastewater treatment sector and is an important tool for leading science and technology *** on the global climate change and the new policies introduced by China,this paper described the new needs for the development of wastewater treatment science and *** offered a retrospective analysis of the historical trajectory of scientific and technological advancements in this *** bibliometrics,it delineated the research hotspots within wastewater treatment,notably highlighting materials genomics,artificial intelligence,and synthetic ***,it posited that,in the future,the field of wastewater treatment should follow the paths of technological innovations with multi-dimensional needs,such as carbon reduction,pollution reduction,health,standardisation,and *** purpose of this paper was to provide references and suggestions for scientific and technological innovations in the field of wastewater treatment,and to contribute to the common endeavor of moving toward a Pollution-Free Planet.

关键词： Wastewater treatment Dual-carbon strategy Technological innovation Carbon reduction

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The characterization of protein lactylation in relation to cardiac metabolic reprogramming in neonatal mouse hearts

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Journal of Genetics and Genomics 2024年第7期51卷 735-748页

作者： Tongyu Zhang Yingxi Zhu Xiaochen Wang Danyang Chong Haiquan Wang Dandan Bu Mengfei Zhao Lei Fang Chaojun Li Ministry of Education Key Laboratory of Model Animals for Disease Study Model Animal Research CenterMedical School of Nanjing UniversityNational Resource Center for Mutant MiceNanjingJiangsu 210093China State Key Laboratory of Reproductive Medicine and Offspring Health China International Joint Research Center on Environment and Human HealthCenter for Global HealthSchool of Public HealthGusu SchoolNanjing Medical UniversityNanjingJiangsu 211166China

In mammals,the neonatal heart can regenerate upon injury within a short time after birth,while adults lose this *** reprogramming has been demonstrated to be critical for cardiomyocyte proliferation in the neonatal ***,we reveal that cardiac metabolic reprogramming could be regulated by altering global protein *** performing 4D label-free proteomics and lysine lactylation(Kla)omics analyses in mouse hearts at postnatal days 1,5,and 7,2297 Kla sites from 980 proteins are identified,among which 1262 Kla sites from 409 proteins are *** clustering analysis reveals that the proteins with altered Kla sites are mainly involved in metabolic *** expression and Kla levels of proteins in glycolysis show a positive correlation while a negative correlation in fatty acid ***,we verify the Kla levels of several differentially modified proteins,including ACAT1,ACADL,ACADVL,PFKM,PKM,and ***,our study reports a comprehensive Kla map in the neonatal mouse heart,which will help to understand the regulatory network of metabolic reprogramming and cardiac regeneration.

关键词： Lactylation Metabolicrep rogramming Postnatal heart regeneration Cell proliferation

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