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Identification and Characteristics Analysis of Micro-Seismic Signals in the Haima Seep Area

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Journal of Earth Science 2024年第1期35卷 288-291页

作者： Xiangchun Wang Bing Nie Zhiyu Wu Weiwei Wang Hua Chen China University of Geosciences Beijing 100083China CNOOC China limited Shenzhen BranchShenzhen 518000China Guangzhou marine Geological Survey Guangzhou 511458China CNOOC China limited Shanghai BranchShanghai 200335China

INTRODUCTION.In recent years,with the steady development of offshore energy exploration technology,submarine gas seepage sites,and their products are gradually being identified and analyzed.The submarine cold seep is a seepage phenomenon in which hydrocarbon gases stored deep in the seafloor are transported to seawater by gushing or seepage under the action of tectonic compression(Roy et al.,2019;Tinivella and Giustiniani,2016).

关键词： submarine Seismic offshore

Evaluation of the Shallow Gas Hydrate Production Based on the Radial Drilling-Heat Injection-Back Fill Method

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Journal of Ocean University of China 2024年第1期23卷 119-128页

作者： CHEN Qiang WAN Yizhao WU Nengyou SUN Jianye WANG Jian LIU Changling LI Yanlong LI Chengfeng HU Gaowei Key Laboratory of Gas Hydrate Ministry of Natural ResourcesQingdao 266071China Laboratory for marine Mineral Resources Laoshan LaboratoryQingdao 266071China CNPC Offshore Engineering Company limited Beijing 100028China Technology Innovation Center for marine Methane Monitoring Ministry of Natural ResourcesQingdao 266071China

It has been evidenced that shallow gas hydrate resources are abundant in deep oceans worldwide.Their geological back-ground,occurrence,and other characteristics differ significantly from deep-seated hydrates.Because of the high risk of well construction and low production efficiency,they are difficult to be recovered by using conventional oil production methods.As a result,this paper proposes an alternative design based on a combination of radial drilling,heat injection,and backfilling methods.Multi-branch holes are used to penetrate shallow gas hydrate reservoirs to expand the depressurization area,and heat injection is utilized as a supplement to improve gas production.Geotechnical information collected from an investigation site close to the offshore production well in the South China Sea is used to assess the essential components of this plan,including well construction stability and gas production behavior.It demonstrates that the hydraulic fracturing of the 60mbsf overburden layer can be prevented by regulating the drilling fluid densities.However,the traditional well structure is unstable,and the suction anchor is advised for better mechanical performance.The gas produc-tion rate can be significantly increased by combining hot water injection and depressurization methods.Additionally,the suitable produc-tion equipment already in use is discussed.

关键词： shallow gas hydrate trail production radial drilling-heat injection-back fill method experimental and numerical simulation

A comparative study of data-driven battery capacity estimation based on partial charging curves

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Journal of Energy Chemistry 2024年第1期88卷 409-420,I0010页

作者： Chuanping Lin Jun Xu Delong Jiang Jiayang Hou Ying Liang Xianggong Zhang Enhu Li Xuesong Mei State Key Laboratory for Manufacturing systems Engineering Xi’an Jiaotong UniversityXi’an 710049ShaanxiChina Shaanxi Key Laboratory of Intelligent Robots School of Mechanical EngineeringXi’an Jiaotong UniversityXi’an 710049ShaanxiChina Department of Electrical Engineering and Automation Luoyang Institute of Science and TechnologyLuoyang 471023HenanChina Wuhan Institute of marine Electric Propulsion China State Shipbuilding Corporation LimitedWuhan 430064HubeiChina Gresgying Digital Technology Ltd. Xi’an 710086ShaanxiChina

With its generality and practicality, the combination of partial charging curves and machine learning(ML) for battery capacity estimation has attracted widespread attention. However, a clear classification,fair comparison, and performance rationalization of these methods are lacking, due to the scattered existing studies. To address these issues, we develop 20 capacity estimation methods from three perspectives:charging sequence construction, input forms, and ML models. 22,582 charging curves are generated from 44 cells with different battery chemistry and operating conditions to validate the performance. Through comprehensive and unbiased comparison, the long short-term memory(LSTM) based neural network exhibits the best accuracy and robustness. Across all 6503 tested samples, the mean absolute percentage error(MAPE) for capacity estimation using LSTM is 0.61%, with a maximum error of only 3.94%. Even with the addition of 3 m V voltage noise or the extension of sampling intervals to 60 s, the average MAPE remains below 2%. Furthermore, the charging sequences are provided with physical explanations related to battery degradation to enhance confidence in their application. Recommendations for using other competitive methods are also presented. This work provides valuable insights and guidance for estimating battery capacity based on partial charging curves.

关键词： Lithium-ion battery Partial charging curves Capacity estimation Data-driven Sampling frequency

Presence of a long nuclear-localization signal sequence in homeodomain transcription factor Nkx 1.2

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Journal of Oceanology and Limnology 2024年第2期42卷 620-626页

作者： Xinyi LI Lihui CHEN Xinyuan WANG Chen SUN Guangdong JI Guobin HU Zhenhui LIU College of marine Life Science and Institute of Evolution&marine Biodiversity Ocean University of ChinaQingdao 266003China Laoshan Laboratory Qingdao 266237China

Homeodomains,a 60-amino acid sequence encoded by 180 nucleotides,are highly conserved DNA-binding motifs that are present in a variety of transcription factors in species ranging from yeast to humans.The NKX proteins belong to the homeodomain(HD)-containing transcription factor family.They play vital roles in the regulation of morphogenesis.NKX1-2 is one member of the NKX subfamily.At present,information about its nuclear localization signal(NLS)sequence is limited.We studied the NLS sequence of zebrafish Nkx1.2 by introducing sequence changes such as deletion,mutation,and truncation,and identified an NLS motif(QNRRTKWKKQ)that is localized at the C-terminus of the homeodomain.Moreover,the deletion of two amino acid residues(RR)in this NLS motif prevents Nkx1.2 from entering the nucleus,indicating that the two amino acids are essential for Nkx1.2 nuclear localization.However,the NLS motif alone is unable to target cytoplasmic protein glutathione S-transferase(GST)to the nucleus.An intact homeodomain is necessary for mediating the complete nuclear transport of cytoplasmic protein.Unlike most nuclear import proteins with short NLS sequences,a long NLS is present in zebrafish Nkx1.2.We also demonstrated that the sequences of homeodomain of NKX1.2 are well conserved among different species.This study is informative to verify the function of the NKX1.2 protein.

关键词： NKX1.2 NKX protein homeodomain nuclear localization signal(NLS) nuclear transport

A semantic segmentation-based underwater acoustic image transmission framework for cooperative SLAM

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Defence Technology（防务技术） 2024年第3期33卷 339-351页

作者： Jiaxu Li Guangyao Han Shuai Chang Xiaomei Fu School of marine Science and Technology Tianjin UniversityTianjin 300072China

With the development of underwater sonar detection technology,simultaneous localization and mapping(SLAM)approach has attracted much attention in underwater navigation field in recent years.But the weak detection ability of a single vehicle limits the SLAM performance in wide areas.Thereby,cooperative SLAM using multiple vehicles has become an important research direction.The key factor of cooperative SLAM is timely and efficient sonar image transmission among underwater vehicles.However,the limited bandwidth of underwater acoustic channels contradicts a large amount of sonar image data.It is essential to compress the images before transmission.Recently,deep neural networks have great value in image compression by virtue of the powerful learning ability of neural networks,but the existing sonar image compression methods based on neural network usually focus on the pixel-level information without the semantic-level information.In this paper,we propose a novel underwater acoustic transmission scheme called UAT-SSIC that includes semantic segmentation-based sonar image compression(SSIC)framework and the joint source-channel codec,to improve the accuracy of the semantic information of the reconstructed sonar image at the receiver.The SSIC framework consists of Auto-Encoder structure-based sonar image compression network,which is measured by a semantic segmentation network's residual.Considering that sonar images have the characteristics of blurred target edges,the semantic segmentation network used a special dilated convolution neural network(DiCNN)to enhance segmentation accuracy by expanding the range of receptive fields.The joint source-channel codec with unequal error protection is proposed that adjusts the power level of the transmitted data,which deal with sonar image transmission error caused by the serious underwater acoustic channel.Experiment results demonstrate that our method preserves more semantic information,with advantages over existing methods at the same compression ratio.It also improves the error

关键词： Semantic segmentation Sonar image transmission Learning-based compression

Integrative analysis of transcriptomic profile reveals potential roles of miRNAs in regulating development of Marsupenaeus japonicas

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Journal of Oceanology and Limnology 2024年第1期42卷 201-215页

作者： Jing WANG Longjun PU Xiaojuan ZHANG Cuicui LIANG Dandan DONG Jiantao GUAN Huarong GUO Ministry of Education Key Laboratory of marine Genetics and Breeding College of Marine Life SciencesOcean University of ChinaQingdao 266003China Institute of Evolution&marine Biodiversity Ocean University of ChinaQingdao 266003China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesQingdao 266071China

Regulation of microRNAs(miRNAs)on various biological processes has been a surprising and exciting field.Identification of miRNAs is the first step to comprehensively understand their functions.However,attempts on global identification and functional verification of miRNAs are very limited in penaeid shrimp Marsupenaeus japonicus,an economically important aquatic species.By performing an integrated analysis of transcriptomic profile from gastrula embryos of M.japonicus,21conserved miRNAs in M.japonicas(mja-miRNAs),belonging to 19 miRNA families,were identified and characterized.Of the 21 mja-miRNAs,15 miRNAs were successfully verified to be predominantly expressed in gastrula stage,where they displayed dynamic expression patterns compared with those in naupliuin stage.Based on perfect or near-perfect match to target region,120 target genes were predicted at transcriptome-wide level.Noteworthy,gene ontology(GO)classification and metabolic pathway annotation revealed eight targets that were actively involved in developmental processes.Of the predicted miRNA-mRNA pairs,six targets were then randomly selected and experimentally validated by dual luciferase reporter assay,where three pairs were proved with potential targeting activity.Overall,to search for conserved miRNAs potentially involved in early development of M.japonicus,we combined in silico and experimental methods,which can be applied in other organisms as well.Our data implied important roles of miRNAs in the early embryonic development and also suggested the presence of complex miRNA-mRNA functional networks in M.japonicus.

关键词： microRNAs(miRNAs) identification Marsupenaeus japonicus embryo transcriptome

Comparative Transcriptome Analysis of Shell-Matrix-Protein Genes and Observation of the Shell Microstructure in the Deep-Sea Clam Calyptogena marissinica

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Journal of Ocean University of China 2024年第3期23卷 797-810页

作者： SHI Yu LIU Dongting YAO Gaoyou HE Maoxian CAS Key Laboratory of Tropical marine Bio-Resources and Ecology Guangdong Provincial Key Laboratory of Applied Marine BiologySouth China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China Southern marine Science and Engineering Guangdong Laboratory(Guangzhou) Guangzhou 511458China University of Chinese Academy of Sciences Beijing 100049China

The deep-sea clam Calyptogena marissinica is widely distributed in the Haima cold seep ecosystem on the northwes-tern slope of the South China Sea with low pH values,low temperature and high pressure.limited information is available on the biomineralization of this species.In this research,we generated a comprehensive transcript dataset of C.marissinica’s mantle tissue,and a total of 19821 unigenes were assembled.Fourteen shell matrix proteins(SMP)-related genes were identified.The qPCR results showed that four out of six prismatic matrix genes(MSP2,MSP5,prisilkin-39,and shematrin),four out of the six nacreous matrix genes(perlucin,pif,pif97,and papilin),and two extrapallial fluid proteins(SPARC and calmodulin)were significantly expressed in the mantle.Both the nacreous and the prismatic layers are chrysanthemum-shaped,which are stacked on the top of each other to form a laminated nacreous structure.The alignment and phylogenetic analysis of MSP-5,Prisilkin-39,Perlucin,and Pif homologues showed that some amino acids of C.marissinica that differed from those detected in other molluscs may cause the different shape of the nacreous and prismatic layers,but do not lead to a change in the species’evolutionary status.These results indicated the conservation of the functions of SMP-related genes in C.marissinica,and the specific shape of the prismatic and nacreous layers of this deep-sea mollusc,which will contribute to the research on the molecular regulation mechanisms of biomineralization in C.marissinica and provide a new perspective to investigate biomineralization in deep-sea clams in general.

关键词： transcriptome biomineralization mantle tissue shell Calyptogena marissinica

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LncRNA pol-lnc78 as a ceRNA regulates antibacterial responses via suppression of pol-miR-n199-3p-mediated SARM down-regulation in Paralichthys olivaceus

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Zoological Research 2024年第1期45卷 25-35页

作者： Xian-Hui Ning Bing Han Ye Peng Shao-Wu Yin College of marine Science and Engineering Nanjing Normal UniversityNanjingJiangsu 210023China CAS Key Laboratory of Experimental marine Biology Center for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdaoShandong 266071China Co-Innovation Center for marine Bio-Industry Technology of Jiangsu Province LianyungangJiangsu 222005China

Long non-coding RNAs(lncRNAs)function as key modulators in mammalian immunity,particularly due to their involvement in lncRNA-mediated competitive endogenous RNA(ceRNA)crosstalk.Despite their recognized significance in mammals,research on lncRNAs in lower vertebrates remains limited.In the present study,we characterized the first immune-related lncRNA(pol-lnc78)in the teleost Japanese flounder(Paralichthys olivaceus).Results indicated that pol-lnc78 acted as a ceRNA for pol-miR-n199-3p to target the sterile alpha and armadillo motif-containing protein(SARM),the fifth discovered member of the Toll/interleukin 1(IL-1)receptor(TIR)adaptor family.This ceRNA network regulated the antibacterial responses of flounder via the Toll-like receptor(TLR)signaling pathway.Specifically,SARM acted as a negative regulator and exacerbated bacterial infection by inhibiting the expression of inflammatory cytokines IL-1βand tumor necrosis factor-α(TNF-α).Pol-miR-n199-3p reduced SARM expression by specifically interacting with the 3’untranslated region(UTR),thereby promoting SARM-dependent inflammatory cytokine expression and protecting the host against bacterial dissemination.Furthermore,pol-lnc78 sponged pol-miR-n199-3p to ameliorate the inhibition of SARM expression.During infection,the negative regulators pol-lnc78 and SARM were significantly down-regulated,while pol-miR-n199-3p was significantly up-regulated,thus favoring host antibacterial defense.These findings provide novel insights into the mechanisms underlying fish immunity and open new horizons to better understand ceRNA crosstalk in lower vertebrates.

关键词： LncRNA SARM miRNA ceRNA Antibacterial response

Transcriptome profiling and RXR gene family identification reveals the molecular mechanism of rapid aging after spawning of cuttlefish Sepiella japonica

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Journal of Oceanology and Limnology 2024年第3期42卷 865-880页

作者： Zhenyu DONG Jiemei ZHAO Feng GUO Shuangrui LIN Huai YANG Yingying YE Changfei CHI Hongfei LI Baoying GUO College of marine Science and Technology Zhejiang Ocean UniversityZhoushan 316022China National Engineering Research Center for marine Aquaculture Zhejiang Ocean UniversityZhoushan 316022China Donghai Laboratory Zhoushan 316021China

Sepiella japonica is a worldwide marine cuttlefish species of high economic value.S.japonica routinely modifying behaviors in reproductive life,such as rapid aging until death after spawning,has been recognized in artificial breeding.However,reproductive behavior at the level of genes is rarely reported,thus,the research on the genetic basis of behavior,reproduction,and artificial breeding was limited.We applied RNA-seq in different stages of reproduction to investigate the reason of rapid aging after spawning,pre-maturity,pre-spawning after maturity,and post-spawning.The retinoid X receptor(RXR)gene family in S.japonica was identified,and 1343–1452 differentially expressed genes(DEGs)in all 3 stages of reproductive life were identified from pairwise m RNA comparisons.Furthermore,through the GO term and KEGG analysis,S.japonica could handle neuronal development and network formation before maturity and have a functional degradation of neural communication,signal transduction,vision,and gene expression after spawning.Eight Sj RXRαs have been identified and they played different roles in growth development or reproduction.Therefore,the regulation of several channels and receptors is the intrinsic molecular mechanism of rapid aging after spawning in S.japonica.This study revealed the survival strategy and provided fundamental data on the level of genes for understanding the reproductive behavior and the reproduction of S.japonica.

关键词： Sepiella japonica RAN-seq retinoid X receptor(RXR)gene family rapid aging intrinsic molecular mechanism