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Study on Formula Optimization of Huangjing Zhima Pills

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Agricultural Biotechnology 2022年第5期11卷 101-106,111页

作者： Jun YE Jinting LIAO Ribin LIU Yupeng ZHANG henry fok college of food science and technology Shaoguan UniversityShaoguan 512005China henry fok college of Biology and Agriculture Shaoguan UniversityShaoguan 512005China Soil and Land Engineering technology Research Center of North Guangdong Shaoguan 512005China

[Objectives]With Polygonati Rhizoma steamed and sundried for 9 times as the main raw material, a type of Huangjing Zhima Pills was developed and its optimal formula was studied. [Methods] The Huangjing Zhima Pills was developed with nine-steamed nine-sundried Polygonati Rhizoma as the main raw material by adding black sesame, black beans, and honey. Taking sensory score and texture properties as evaluation indexes, the optimal formula parameters of Huangjing Zhima Pills were determined by single factor experiments and an orthogonal test combined with a formula scoring method. [Results] The best formula of Huangjing Zhima Pills was addition amount of nine-steamed nine-sundried Polygonati Rhizoma 25%, addition amount of black bean powder 15%, addition amount of honey added 17%, and addition amount of black sesame powder 30%. The moisture content in the finished product was(13.367±0.056) g/100 g, and the total colony count, Escherichia coli, mold and yeast and other microbial indexes all met the standards. The Huangjing Zhima Pills prepared under these conditions had pleasant sesame flavor and Polygonati Rhizoma flavor, delicate and sweet tasted, and a slight aftertaste of sweetness. Meanwhile, Polygonatum, black sesame and black beans are all medicinal and food homologous plants with certain health care effects, and the Huangjing Zhima Pills prepared from them are healthy snacks that people can carry and eat easily. [Conclusions] This study further enhances the added value of Polygonatum and provides a new way for the high-value utilization of Polygonatum.

关键词： Polygonati Rhizoma Zhima pill Processing

Effects of Nutrients on Growth and Yield of Shatian Pomelo Trees with Huanglongbing

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Agricultural Biotechnology 2020年第5期9卷 39-43页

作者： Jun YE Wei ZHI Yupeng ZHANG Xiaoyuan CHEN Chongjian MA henry fok college of food science and technology Shaoguan UniversityShaoguan 512005China henry fok college of Biology and Agriculture Shaoguan UniversityShaoguan 512005China Soil and Land Engineering technology Research Center of North Guangdong Shaoguan 512005China

[Objectives]In order to effectively alleviate Huanglongbing of Shatian pomelo trees,a nutrient preparation was developed and its effect on the growth and yield of Shatian pomelo was explored.[Methods]A field experiment was carried out with four treatments:control group,nutrient solution group,agent group,and nutrient solution+agent group,and the liquids were infused into the fruit trees through the trunk using a hanging bag.[Results]Through field trials,it was found that the nutrient solution treatment of fruit trees significantly increased the leaf chlorophyll content and nitrogen content,which increased by 17%and 12%compared with the control group,respectively,reaching(70.80±1.57)and(5.53±0.1)mg/kg,respectively;and the combined application of the nutrient solution and powder agents greatly alleviated the symptoms of Huanglongbing in Shatian pomelo plants,and significantly improved the bacterial disease index,photosynthetic intensity and yield,which increased by 152%,158%and 213.53%respectively,reaching(371.00±7.22),(406.67±7.09)and 23.15 kg/hm^2.[Conclusions]The use of the nutrient solution and agents greatly alleviated the symptoms of Huanglongbing of Shatian pomelo plants,and significantly improved the chlorophyll content,nitrogen content,bacterial disease index and photosynthetic ***,the number of fruit per plant and the weight of single fruit were better than other treatment groups and the control group,the technical operation was simple and the effect was *** provides a certain reference basis for the prevention and control measures of Huanglongbing in Shatian pomelo plants.

关键词： Shatian pomelo Huanglongbing Nutrient Fruit quality

Dynamic changes of peptidome and release of polysaccharide in sea cucumber(Apostichopus japonicus) hydrolysates depending on enzymatic hydrolysis approaches

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food science and Human Wellness 2022年第5期11卷 1331-1341页

作者： Yanchao Wang Yan Song Yaoguang Chang Yanyan Liu Guangning Chen Changhu Xue college of food science and Engineering Ocean University of ChinaQingdao 266003China Laboratory for Marine Drugs and Bioproducts Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China

Enzymatic hydrolysis has been widely used to produce bioactive hydrolysates from sea cucumber body ***,inspired by the clarification of Apostichopus japonicus genome,we investigated the enzymatic hydrolysis of sea cucumber body wall by using the omics *** proteins,including major yolk proteins,collagens,extracellular matrix glycoproteins and muscle proteins,were released from the body wall by different hydrolysis condition.A portfolio of 216 shared peptides were detected in the peptidome by papain with different hydrolysis time,while 32 shared peptides were detected in the peptidome by differing *** peptides and the relative abundance distribution profiles of shared peptides changed depending on hydrolysis approaches,indicating dynamic changes of peptidome during ***,release of sulfated fucan and fucosylated chondroitin sulfate changed with the hydrolysis *** monitoring of dynamic enzymatic hydrolysis process at a molecular scale would contribute to production and quality control of sea cucumber hydrolysates.

关键词： Sea cucumber Enzymatic hydrolysis Protein Peptidome Polysaccharide

Photoinduced palladium-catalyzed 1,3-diene-selective fluoroalkylamination compounds as potential bactericidal agent against Xanthomonas oryzae ***

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Chinese Chemical Letters 2024年第1期35卷 401-405页

作者： Yu Shi Zhao-Sheng Zhang Jiang Shao Chen Fu Lan-Tu Xiong Zhao-Dong Li Zi-Ning Cui National Key Laboratory of Green Pesticide Integrative Microbiology Research CentreGuangdong Province Key Laboratory of Microbial Signals and Disease ControlCollege of Plant ProtectionSouth China Agricultural UniversityGuangzhou 510642China henry fok School of Biology and Agriculture Shaoguan UniversityShaoguan 512005China college of Materials and Energy South China Agricultural UniversityGuangzhou 510642China

A series of photoinduced palladium-catalyzed 1,3-diene-selective fluoroalkylamination derivatives was rationally synthesized based on diversity-oriented synthesis via cross coupling of 1,3-dienes,amines and fluoroalkyl *** reaction featured good function group tolerance and a broad substrate scope,which could be extended to the late-stage modification of bioactive *** activity of all the compounds against Xanthomonas oryzae ***(Xoo)was *** them,compound E14 showed significant activity against Xanthomonas oryzae ***(Xoo)with half maximal effective concentration(EC50)value of 6.61μmol/*** pot experiments,the results showed that E14 could control rice bacterial blight with protective and curative efficiencies of 37.5%and 63.2%at 200μg/mL,***,a plausible mechanism for antibacterial behavior of E14 was proposed by electron microscopy,flow cytometry,reactive oxygen species detection,and biofilm *** current work,it can promote the development of photoinduced palladium-catalyzed 1,3-diene-selective fluoroalkyl amination compounds as prospective antibacterial agent bearing an intriguing mode of action.

关键词： 1,3-Diene-selective fluoroalkylamination derivatives Xanthomonas oryzae pv.oryzae Antibacterial activity Electron microscopy Reactive oxygen Flow cytometry

Removal of the main inflorescence to induce reflowering of loquat

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Horticultural Plant Journal 2022年第1期8卷 35-43页

作者： Jiangrong Peng Wenkun Li Yuan Yuan Zhiqiang Han Yunpeng Cao Muhammad Qasim Shahid Zhike Zhang Yongshun Gao Shunquan Lin Yuanyuan Jiang henry fok college of Biology and Agriculture Shaoguan UniversityShaoguanGuangdong 512005China State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources College of HorticultureSouth China Agricultural UniversityGuangzhou 510642China The Key Laboratory of Cultivation and Protection for Non-wood Forest trees Ministry of EducationCentral South University of Forestry and TechnologyChangsha 410004China college of Agriculture South China Agricultural UniversityGuangzhou 510642China Beijing Engineering Research Center for Strawberry Beijing 100093China Beijing Academy of Forestry and Pomology sciences Beijing 100093China

Loquat(Eriobotrya japonica Lindl.)is an evergreen fruit tree species of the Rosaceae,and its unique flowering time greatly hinders its *** explore the artificial regulation of loquat flowering time,we removed the main inflorescence(by cutting it)to induce *** different loquat tree cultivars with different stages,the inflorescence was removed by cutting the main floral axis at two alternative positions:the upper or the lower position beneath the inflorescence,and it was found that the proportion of reflowering resulting from removing the upper position of the main floral axis of yellow-flesh loquat cultivars during the full-bloom stage was the *** addition,compared with those of the normal-growing panicles,the number of flower buds and branch axes of the reflowering panicles decreased significantly after ***,these newly produced inflorescences flowered 2–4 months later than normal-growing inflorescences did,effectively prolonging both the flowering and fruiting *** addition,qRT-PCR results showed that EjFT1,EjFT2,EjAP1–1 and EjAP1–2 were highly expressed in the floral *** findings highlighted a new method for extending the production cycle of loquat and provided a reference for the flowering regulation of loquat and other economically important fruit tree species.

关键词： Eriobotrya japonica Reflowering Flowering time Cutting Production cycle

Coexpression network analysis reveals an MYB transcriptional activator involved in capsaicinoid biosynthesis in hot peppers

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Horticulture Research 2020年第1期7卷 756-769页

作者： Binmei Sun Xin Zhou Changming Chen Chengjie Chen Kunhao Chen Muxi Chen Shaoqun Liu Guoju Chen Bihao Cao Fanrong Cao Jianjun Lei Zhangsheng Zhu Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(South China) Ministry of Agriculture and Rural AffairsCollege of HorticultureSouth China Agricultural UniversityGuangzhou 510642China Jiangxi Agricultural Engineering college Zhangshu 331200 JiangxiChina Guangdong Helinong Seeds Co.Ltd.Shantou 515800 GuangdongChina Guangdong Helinong Agricultural Research Institute Co.Ltd.Shantou 515800 GuangdongChina henry School of Agricultural science and Engineering Shaoguan UniversityGuangdong 512005China PekingUniversity—Southern University of science and technology Joint Institute of Plant and food sciences Department of BiologySouthern University of Science and TechnologyShenzhen 518055China

Plant biosynthesis involves numerous specialized metabolites with diverse chemical natures and biological *** biosynthesis of metabolites often exclusively occurs in response to tissue-specific combinatorial developmental cues that are controlled at the transcriptional *** are a group of specialized metabolites that confer a pungent flavor to pepper *** biosynthesis occurs in the fruit placenta and combines its developmental *** the capsaicinoid biosynthetic pathway has been largely characterized,the regulatory mechanisms that control capsaicinoid metabolism have not been fully *** this study,we combined fruit placenta transcriptome data with weighted gene coexpression network analysis(WGCNA)to generate coexpression networks.A capsaicinoid-related gene module was identified in which the MYB transcription factor CaMYB48 plays a critical role in regulating capsaicinoid in *** biosynthetic gene(CBG)and CaMYB48 expression primarily occurs in the placenta and is consistent with capsaicinoid ***48 encodes a nucleus-localized protein that primarily functions as a transcriptional activator through its C-terminal activation ***48 regulates capsaicinoid biosynthesis by directly regulating the expression of CBGs,including AT3a and *** together,the results of this study indicate ways to generate robust networks optimized for the mining of CBG-related regulators,establishing a foundation for future research elucidating capsaicinoid regulation.

关键词： pepper specialized network

Flowering-time regulation by the circadian clock:From Arabidopsis to crops

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The Crop Journal 2024年第1期12卷 17-27页

作者： Mingkang Yang Wenjie Lin Yarou Xu Biyu Xie Baiyin Yu Liang Chen Wei Huang State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources College of Life SciencesSouth China Agricultural UniversityGuangzhou 510642GuangdongChina henry fok School of Biology&Agriculture Shaoguan UniversityShaoguan 512005GuangdongChina Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms South China Agricultural UniversityGuangzhou 510642GuangdongChina Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou 510642GuangdongChina Guangdong Provincial Key Laboratory of Utilization and Conservation of food and Medicinal Resources in Northern Region Shaoguan UniversityShaoguan 512005GuangdongChina

Precise timing of flowering in plants is critical for their growth and reproductive *** factor controlling flowering time is the cycle of light and darkness within a day,known as the *** are classified into long-day,short-day,and day-neutral plants based on light requirements for floral *** the molecular mechanisms that govern this differentiation remain incompletely understood,studies have consistently shown that the circadian clock plays a central role in regulating photoperiod response across diverse plant ***,there is a scarcity of reviews describing the regulatory network linking the circadian clock with photoperiodic *** review summarizes that regulatory network,focusing on the distinct roles of clock genes in long-day and short-day *** also discuss the strategies of clock gene mutations contributing to geographic variation in longday and short-day crops.

关键词： Circadian clock Photoperiod Short-day plants Long-day plants

In situ sulfur-doped mesoporous tungsten oxides for gas sensing toward benzene series

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Chinese Chemical Letters 2024年第7期35卷 502-507页

作者： Yu Deng Yan Liu Yonghui Deng Jinsheng Cheng Yidong Zou Wei Luo State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and EngineeringDonghua UniversityShanghai 201620China Department of Chemistry State Key Laboratory of Molecular Engineering of PolymersShanghai Key Laboratory of Molecular Catalysis and Innovative MaterialsiChEMFudan UniversityShanghai 200433China Department of Polymeric Materials School of Materials Science and EngineeringTongji UniversityShanghai 201804China henry-Fork School of food sciences Shaoguan UniversityShaoguan 512005China State Key Lab of Transducer technology Shanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai 200050China

Benzene series as highly toxic gases have inevitably entered human life and produce great threat to human health and ecological environment,and thus it is distinctly meaningful to monitor benzene series with quickly,real-time and efficient ***,novel sulfur-doped mesoporous WO_(3)materials were synthesized via classical in-situ solvent evaporation induced co-assembly strategy combined with doping engineering,which possessed highly crystallized frameworks,high specific surface area(40.9–63.8 m^(2)/g)and uniform pore size(~18 nm).Benefitting from abundant oxygen vacancy and defects via S-doping,the tailored mesoporous S/m WO_(3)exhibited excellent benzene sensing performance,including high sensitivity(50 ppm vs.48),low detection limit(ca.500 ppb),outstanding selectivity and favorable *** addition,the reduction of band gap resulted from S-doping promotes the carrier migration in the sensing materials and the reaction at the gas–solid sensing *** provides brand-new approach to design sensitive materials with multiple reaction sites.

关键词： Mesoporous materials Benzene series Tungsten oxides Sulfur doping Gas sensor

A 1-bp deletion in the MC04g1399 is highly associated with failure to produce fruit wart in bitter gourd

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Horticultural Plant Journal 2024年第1期10卷 171-180页

作者： Jia Liu Junjie Cui Jichi Dong Jian Zhong Chunfeng Zhong Fanchong Yuan Wendong Guan Fang Hu Jiaowen Cheng Kailin Hu college of Horticulture South China Agricultural UniversityGuangzhouGuangdong 510642China Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(South China) Ministry of Agriculture and Rural AffairsGuangzhouGuangdong 510642China Guangdong Vegetables Engineering Research Center GuangzhouGuangdong 510642China Department of Horticulture Foshan UniversityFoshanGuangdong 528225China henry fok School of Biology and Agricultural Shaoguan UniversityShaoguanGuangdong 512023China

Fruit wart is an important appearance trait influencing consumer preferences of bitter gourd(Momordica charantia L.).The molecular genetic mechanisms underlying fruit wart formation in bitter gourd are largely *** this study,genetic analysis based on four generations showed that fruit wart formation in bitter gourd was controlled by a single dominant locus named as *** Fwa locus was initially mapped into a 4.82 Mb region on pseudochromosome 4 by BSA-seq analysis and subsequently narrowed down to a 286.30 kb region by linkage analysis.A large F2population consisting of 2360 individuals was used to screen recombinants,and the Fwa locus was finally fine mapped into a 22.70 kb region harboring four protein-coding genes through recombination ***04g1399,encoding an epidermal patterning factor 2-like protein,was proposed as the best candidate gene for Fwa via sequence variation and expression *** addition,a 1-bp insertion and deletion(InDel)variation within MC04g1399 was converted to a cleaved amplified polymorphic sequence(CAPS)marker that could precisely distinguish between the warty and non-warty types with an accuracy rate of 100%among a wide panel of 126 bitter gourd germplasm *** results not only provide a scientific basis for deciphering the molecular mechanisms underlying fruit wart formation but also provide a powerful tool for efficient genetic improvement of fruit wart via marker-assisted selection.

关键词： Bitter gourd Fruit wart Bulk segregant analysis Fine-mapping Candidate gene