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Rapid Identification of a Candidate Gene Related to Fiber Strength Using a Superior Chromosome Segment Substitution Line from Gossypium hirsutum × Gossypium barbadense via Bulked Segregant RNA-Sequencing

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Phyton-International Journal of Experimental Botany 2021年第3期90卷 837-858页

作者： Qi Zhang Pengtao Li Aiying Liu Shaoqi Li Quanwei Lu Qun Ge Junwen Li Wankui Gong Xiaoying Deng Haihong Shang Yuzhen Shi Youlu Yuan State Key Laboratory of co.ton Biology Key Laboratory of Biological and Genetic Breeding of CottonThe Ministry of AgricultureInstitute of Cotton ResearchChinese Academy of Agricultural SciencesAnyang455000China School of Biotechnology and Food engineering Anyang Institute of TechnologyAnyang455000China Zhengzhou Research Base State Key Laboratory of Cotton BiologyZhengzhou UniversityZhengzhou450001China

co.ton is the most widely cultivated co.mercial crop producing natural fiber around the *** a critical trait for fiber quality,fiber strength principally determined during the seco.dary wall thickening *** on the developed BC5F3:5 CSSLs(chromosome segment substitution lines)from Gossypium hirsutum CCRI36×*** Hai 1,the superior MBI9915 was chosen to co.struct the seco.dary segregated population BC7F2 with its recurrent parent CCRI36,which was subsequently subjected to Bulk segregant RNA-sequencing(BSR-seq)for rapid identification of candidate genes related to fiber strength.A total of 4 fiber-transcriptome libraries were separately co.structed and sequenced,including two parents(CCRI36 and MBI9915)and two extreme pools at 20 DPA(days post anathesis).Through multiple co.parisons,536 DEGs(differentially expressed genes)were overlapped at 20 ***-polymorphism co.parison in mRNA sequences revealed 831 highly probable SNPs between two extreme pools related to fiber *** analysis was performed between two extreme pools with SNP-index *** co.related regions with 1981 annotation genes were obtained between two pools at 20 DPA,of which 12 co.mon DEGs were similarly identified both between two parents and two *** gene(Gh_A07G0837)in the candidate region related to fiber strength was differentially expressed in both parents and extreme pools and involved in fiber strength development through reactive oxygen species(ROS)***-expression analysis of Gh_A07G0837 showed that Gh_A07G0837 may co.perate with other genes to regulate fiber *** reliability of BSR-seq results was validated by the quantitative real-time PCR(qRT-PCR)experiments on 5 co.mon DGEs 20 ***-expressed analysis results indicated that there were some genes expressed especially low in MBI9915,resulting in good fiber *** on bulked segregant analysis on the extreme pools derived from superior CSSL population,this study indicates

关键词： co.ton Fiber strength CSSLs BSR-seq gene clone co.expressed analysis

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co.localization of two-co.or rAAV2-retro co.firms the dispersion characteristics of efferent projections of mitral cells in mouse accessory olfactory bulb

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Zoological Research 2020年第2期41卷 148-156页

作者： Ning Zheng Zhi-Zhong Wang Song-Wei Wang Fang-Jia Yang Xu-Tao Zhu Chen Lu Anne Manyande Xiao-Ping Rao Fu-Qiang Xu Center of Brain Science State Key Laboratory of Magnetic Resonance and Atomic and Molecular PhysicsNational Center for Magnetic Resonance in WuhanKey Laboratory of Magnetic Resonance in Biological SystemsWuhan Institute of Physics and MathematicsInnovation Academy for Precision Measurement Science and TechnologyChinese Academy of SciencesWuhanHubei 430071China University of the Chinese Academy of Sciences Beijing 100049China Department of Automation School of Electrical EngineeringZhengzhou UniversityZhengzhouHenan 450001China School of Life Science Wuhan UniversityWuhanHubei 430072China School of Human and Social Sciences University of West LondonMiddlesex TW89GAUK Divisions of Biomedical Photonics Wuhan National Laboratory for OptoelectronicsWuhanHubei 430074China

The accessory olfactory bulb(AOB), located at the posterior dorsal aspect of the main olfactory bulb(MOB), is the first brain relay of the accessory olfactory system(AOS), which can parallelly detect and process volatile and nonvolatile social chemosignals and mediate different sexual and social behaviors with the main olfactory system(MOS). However, due to its anatomical location and absence of specific markers, there is a lack of research on the internal and external neural circuits of the AOB. This issue was addressed by singleco.or labeling and fluorescent double labeling using retrograde rAAVs injected into the bed nucleus of the stria terminalis(BST), anterior co.tical amygdalar area(Aco., medial amygdaloid nucleus(MeA), and posteromedial co.tical amygdaloid area(PMco. in mice. We demonstrated the effectiveness of this AOB projection neuron labeling method and showed that the mitral cells of the AOB exhibited efferent projection dispersion characteristics similar to those of the MOB. Moreover, there were significant differences in the number of neurons projected to different brain regions, which indicated that each mitral cell in the AOB co.ld project to a different number of neurons in different co.tices. These results provide a circuitry basis to help understand the mechanism by which pheromone information is enco.ed and deco.ed in the AOS.

关键词： Accessory olfactory bulb Efferent projections Retrograde rAAVs Projection neuron labeling Dispersion characteristics Circuitry basis

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珊瑚礁钙质砂微观结构分析

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河南科技大学学报（自然科学版） 2021年第6期42卷 6-11,18,M0002页

作者：安晓宇王斐左殿军朱前林天津大学水利工程仿真和安全国家重点试验室天津300072 交通运输部天津水运工程科学研究所天津300456 煤基co.(2)捕集与地质储存重点实验室江苏徐州221008

针对珊瑚礁钙质砂的特殊结构可能影响钙质砂的压缩、剪切和破碎等力学性能这一问题,通过X射线衍射和X射线断层扫描等技术手段,研究了岛礁珊瑚礁钙质砂的物相组成、表面结构和内部结构等特征。研究结果表明:钙质砂物质组成以生物文石为主... 详细信息

针对珊瑚礁钙质砂的特殊结构可能影响钙质砂的压缩、剪切和破碎等力学性能这一问题,通过X射线衍射和X射线断层扫描等技术手段,研究了岛礁珊瑚礁钙质砂的物相组成、表面结构和内部结构等特征。研究结果表明:钙质砂物质组成以生物文石为主,镁方解石次之。钙质砂颗粒中的原生海洋生物碎屑较多,并保留有大量的原生构造,超过50%为珊瑚礁破碎、崩解和风化后的产物。钙质砂孔隙非常发育,孔隙差异性非常大,内孔隙率较大。孔隙率不仅取决于孔道直径,还取决于孔道的密集程度。

关键词：钙质砂 X射线衍射扫描电镜 X射线断层扫描微观结构

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