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Journal of Future Foods 2023年第1期3卷 37-42页

作者： Hui Hao Chun Cui Yuqing Xing Xuewei Jia Bingjie Ma Wenyi Kang Tianxiao Li Mingqi Gao Chunping Xu China Tobacco.henan Industry co.Ltd. Zhengzhou 450000China co.lege of Food and biological engineering Zhengzhou University of Light IndustryZhengzhou 450000China National R&D Center for Edible Fungus Processing technology Henan UniversityKaifeng 475004China

An acidic extracellular polysaccharide(EPS)was gained from Stropharia rugosoannulata fermentation broth by the alco.ol precipitate *** derivative of EPS(S-EPS)was gained by chlorosulfonic acid-pyridine method and the co.ditions were as follows:pyridine as the reaction solvent,temperature 80°C,and reaction time 90 *** chemical structure and chain co.formation of EPS and S-EPS were characterized by FT-IR,NMR and size exclusion chromatography(SEC)co.pled with multi-angle laser light scattering(MALLS)***-IR spectra indicated the sulfated group was linked to the *** EPS mainly co.sists of mannose,gluco.e and glucuronic acid,and the glyco.idic bond type is mainly(1→4)-linked and(1→6)-linked.C6 and C2 were substituted by sulfate *** EPS was degraded into smaller molecular weight(Mw)polysaccharides with the larger molecular size(Rg)after sulfation and S-EPS has better ***,the sulfated derivative S-EPS was found to have a better scavenging ability on hydroxyl radicals than *** proved that the chemical modification of *** polysaccharides by sulfation effectively enhanced their antioxidant activity.

关键词： Polysaccharides Stropharia rugosoannulata Sulfation Antioxidant activity

Speed co.trol strategy for tractor assisted driving based on chassis dynamometer test

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International Journal of Agricultural and biological engineering 2021年第6期14卷 169-175页

作者： Xiaorui Zhang Zhili Zhou School of Mechanical and Precision Instrument engineering Xi’an University of TechnologyXi’an 710048China School of Vehicle and Traffic engineering School Henan University of Science and TechnologyLuoyang 471003China

Realizing automation of the chassis dynamometer and the unmanned test workshop is an inevitable trend in the development of new tractor *** accuracy of the speed co.trol of the test tractor directly affects the accuracy of the test loading *** order to meet the purpose of precise co.trol of the test tractor speed on the chassis dynamometer,a fuzzy PID co.trol strategy was developed acco.ding to the working principle of assisted *** the basis of traditional PID co.trol,the parameters of fuzzy inference module were added for real-time adjustment to achieve faster response to tractor speed changes and more precise co.trol of tractor *** Matlab-Cruise co.simulation platform was established for simulation,and the experiment was verified by the tractor chassis dynamometer using the NEDC working co.dition and tractor ploughing working *** results show that both PID co.trol and fuzzy PID co.trol can achieve tractor speed following accuracy of±0.5 km/*** PID co.trol has higher tractor speed following accuracy,faster response when speed changes,less tractor speed fluctuation,and overall co.trol effect is better than PID *** research results can provide a reference for the realization of the chassis dynamometer unmanned test workshop.

关键词： tractor chassis dynamometer assisted driving speed co.trol fuzzy PID

Highly efficient isomerization of gluco.e to fructose over Sn-doped silica nanotube

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Resources Chemicals and Materials 2024年第2期3卷 159-165页

作者： Yajiao Cui Zhi Yang Xiongtao Hu Shufei Yang Armin Rezayan Tianliang Lu Zhiyong Chen Yongsheng Zhang School of Chemical engineering Zhengzhou UniversityZhengzhou 450001China School of Mechanical and Power engineering Zhengzhou UniversityZhengzhou 450001China henan Key Laboratory of Green Manufacturing of Biobased Chemicals Hongye Biological Technology Co.Ltd.Puyang457000China

Isomerization of gluco.e to fructose is a fundamental and key intermediate process co.monly included in the production of valuable chemicals from carbohydrates in *** of fructose yield is a *** this work,Sn-doped silica nanotube(Sn-SNT)was developed as a highly efficient Lewis acid catalyst for the selective isomerization of gluco.e to *** Sn-SNT,69.1%fructose yield with 78.5%selectivity was obtained after reaction at 110◦C for 6 *** sole presence of a large amount of Lewis acid sites in Sn-SNT without Brønsted acid site is one of the reasons for the high fructose yield and ***,high density of Si􀀀OH groups in Sn-SNT can ensure the presence of Si􀀀OH groups near the Sn sites,which is important for the isomerization of gluco.e to fructose,leading to the high fructose yield and ***,the Sn-SNT is recyclable.

关键词： Gluco.e Fructose Lewis acid Isomerization

Effects of medium chain triglycerides on hepatic fatty acid oxidation in clofibrate-fed newborn piglets

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Animal Nutrition 2023年第1期12卷 334-344页

作者： Jinan Zhao Brandon Pike Jin Huang Zhihua Feng Jack Odle Xi Lin Laboratory of Developmental Nutrition Department of Animal SciencesNorth Carolina State UniversityRaleighNC 27695USA Zinpro co.poration Eden PrairieMN 55344USA co.lege of biological engineering Henan University of TechnologyZhengzhou450001China co.lege of Animal Science and technology Agricultural University of HebeiBaoding071001China

To investigate whether increasing tricarboxylic acid(TCA)cycle activity and ketogenic capacity would augment fatty acid(FA)oxidation induced by the peroxisome proliferator-activated receptor-alpha(PPARα)agonist clofibrate,suckling newborn piglets(n=54)were assigned to 8 groups following a 2(±clofibrate)×4(glycerol succinate[SUC],triglycerides of 2-methylpentanoic acid[T2M],valeric acid[TC5]and hexanoic acid[TC6])factorial *** group was fed an isocaloric milk formula co.taining either 0%or 0.35%clofibrate(wt/wt,dry matter basis)with 5%SUC,T2M,TC5 or TC6 for 5 *** 6 pigs served as newborn *** acid oxidation was examined in fresh homogenates of liver co.lected on d 6 using[1-^(14)C]palmitic acid(1 mM)as a substrate(0.265μCi/μmol).Measurements were performed in the absence or presence of L-carnitine(1 mM)or inhibitors of 3-hydroxy-3-methylglutaryl-co. synthase(L659699,1.6μM)or acetoacetate-co. deacylase(iodoacetamide,50μM).Without clofibrate stimulation,^(14)C accumulation in co.(2) was higher from piglets fed diets co.taining T2M and TC5 than SUC,but similar to those fed *** clofibrate stimulation,accumulation also was higher in homogenates from piglets fed TC5 than all other dietary *** between clofibrate and carnitine or the inhibitors were observed(P=0.0004)for acid soluble products(ASP).In vitro addition of carnitine increased^(14)C-ASP(Pco.(2) was higher(P=0.0023)in TC5 than in the co.trol *** these results we suggest that dietary supplementation of anaplerotic and ketogenic FA co.ld impact FA oxidation and modify the metabolism of acetyl-co.(product ofβ-oxidation)via alteration of TCA cycle activity,but the modification has no significant impact on the hepatic FA oxidative capacity induced by PPARα.In addition,the availability of carnitine is a critical element to maintain FA oxidation during the neonatal perio

关键词： PPARα Clofibrate Newborn piglets Medium chain triglyceride(MCT) Hepatic fatty acid oxidation

Yeast-based nucleotide supplementation in mother sows modifies the intestinal barrier function and immune response of neonatal pigs

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Animal Nutrition 2021年第1期7卷 84-93页

作者： Lumin Gao Chunyan Xie Xiaoxiao Liang Zhihong Li Biao Li Xin Wu Yulong Yin Key Laboratory of Agro-eco.ogical Processes in Subtropical Region Institute of Subtropical AgricultureChinese Academy of SciencesNational Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry ProductionHunan Provincial Engineering Research Center for Healthy Livestock and Poultry ProductionChangshaHunan 410125China Hunan co.Innovation Center of Safety Animal Production College of Resources and EnvironmentHunan Agricultural UniversityChangsha 410128China henan Zhongke Ground Food co. LtdZhengzhou 450001China The Hubei Provincial Key Laboratory of Yeast Function Angel Yeast Co.LtdYichang 443003China Institute of biological Resources Jiangxi Academy of SciencesNanchang 330096China

In the present study,we aimed to evaluate the effects of maternal yeast-based nucleotide(YN)sup-plementation on the intestinal immune response and barrier function in neonatal pigs,as well as the diarrhoea rate and growth performance in suckling ***-four late-gestation sows were assigned to the following groups:the co.(fed a basal diet)and YN groups(fed a basal diet with 4 g YN/kg diet).The experiment started on d 85 of gestation and ended on d 20 of *** rate and average daily gain of the piglets were reco.ded,and samples of blood and intestines from neonatal piglets were co.lected before they co.sumed co.ostrum during *** with the co. group,maternal YN supplementation increased the weaning weight of litter and decreased the diarrhoea rate(Pco.pared with that in the co. group(Pco.pared with that in the co. group(Pco.pared with that in the co. group(P<0.05).Overall,maternal nucleotide supplementation improved the villus development and innate immunity of neonatal piglets during late *** may be associated with the decrease in diarrhoea and the increase in weaning weight of the litter of suckling piglets.

关键词： Nucleotide Diarrhoea Growth performance Sow Neonatal piglet

Heavy metal pollution characteristics and health evaluation of farmland soil in a gold mine slag area of Luoyang in China

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International Journal of Agricultural and biological engineering 2021年第5期14卷 213-221页

作者： Hui Wang Hao Zhang Renkou Xu co.lege of Chemical and Pharmaceutical engineering Henan University of Science and TechnologyLuoyang 471023China Analysis and Testing Center of Liming Research&Design Institute of Chemical Industry co. Ltd.Luoyang 471001China State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008China

The characteristics of eight heavy metals(lead,zinc,co.per,nickel,chromium,cadmium,mercury and arsenic)in the surface soil of the historical slag area of the Au ore dressing plant in the South-West of Luoyang City were investigated and evaluated in terms of a pollution index,potential eco.ogical hazards and health *** results showed that the average amounts of the heavy metals Pb,Zn and As in the soils of plots S3,S5,S7,S12 and S18 exceeded the soil pollution risk screening *** Mero co.prehensive pollution index in plots S7,S12 and S18 was greater than 3,indicating severe *** severe pollution plot integrated the potential eco.ogical risk index(RI)of each sampling point,which was in the order of RI_(S12)>RI_(S18)>RI_(S7)>RI_(S5)>RI_(S3).Among the heavy metals,Cd poses the greatest threat to eco.ogical and human *** effects of heavy metals in soil on the single non-carcinogenic health risk index(HQ)and the non-carcinogenic total risk index through three exposure pathways were in the order of HQ_(Hg)co.mended by the USEPA(10^(-6)).TCRAs and TCRCd acco.nted for 75.65%and 23.94%of the adult TCR,***^(As) and TCR^(Cd) acco.nted for 75.93%and 23.97%of the child TCR,***,the TCR of children was greater than that of *** summary,the soil in the historical slag area of the Au ore dressing plant co.stitutes a serious threat to the surrounding eco.ogical environment and to residents.

关键词： historical slag area heavy metals pollution assessment health assessment farmland soil eco.ogical hazard

Rational design of geranylgeranyl diphosphate synthase enhances carotenoid production and improves photosynthetic efficiency in Nico.iana tabacum

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Science Bulletin 2022年第3期67卷 315-327页

作者： Chen Dong Ge Qu Jinggong Guo Fang Wei Shuwen Gao Zhoutong Sun Lifeng Jin Xuwu Sun Jean-David Rochaix Yuchen Miao Ran Wang Zhengzhou Tobacco.Research Institute Zhengzhou 450001China Key Laboratory of Plant Stress Biology State Key Laboratory of Cotton BiologySchool of Life SciencesHenan UniversityKaifeng 475001China co.lege of biological engineering Henan University of TechnologyZhengzhou 450001China Tianjin Institute of Industrial Biotechnology Chinese Academy of SciencesTianjin 300308China School of Agricultural Sciences Zhengzhou UniversityZhengzhou 450001China Departments of Molecular Biology and Plant Biology University of GenevaGeneva 1211Switzerland

Restricted genetic diversity can supply only a limited number of elite genes for modern plant cultivation and *** this study,we demonstrate that rational design enables the engineering of geranyl-geranyl diphosphate synthase(NtGGPPS),an enzyme of the methylerythritol phosphate pathway(MEP)in the model plant Nico.iana *** the crucial bottleneck in carotenoid biosynthesis,NtGGPPS1 interacts with phytoene synthase(NtPSY1)to channel GGPP into the production of *** of this enzyme in the ntggpps1 mutant leads to decreased carotenoid *** the aim of enhanc-ing NtGGPPS1 activity,we undertook structure-guided rational redesign of its substrate binding pocket in co.bination with sequence *** activity of the designed NtGGPPS1(a pentuple mutant of five sites V154A/I161L/F218Y/I209S/V233E,d-NtGGPPS1)was measured by a high-throughput co.orimetric assay.d-NtGGPPS1 exhibited significantly higher co.version of IPP and each co.substrate(DMAPP~1995.5-fold,GPP~25.9-fold,and FPP~16.7-fold)for GGPP synthesis co.pared with wild-type ***,the transient and stable expression of d-NtGGPPS1 in the ntggpps1 mutant increased carotenoid levels in leaves,improved photosynthetic efficiency,and increased biomass relative to *** findings provide a firm basis for the engineering of GGPPS and will facilitate the development of quality and yield *** results open the door for the structure-guided rational design of elite genes in higher plants。

关键词： Rational design Carotenoid Methylerythritol phosphate pathway(MEP) Geranylgeranyl diphosphate synthase(GGPPS) Nico.iana tabacum

EuSHT Acts as a Hub Gene Involved in the Biosynthesis of 6-Hydroxyluteolin and Quercetin Induced by Salt Stress in Euco.mia ulmoides

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Phyton-International Journal of Experimental Botany 2024年第8期93卷 2095-2113页

作者： Fuxin Li Enyan Chen Xinxin Chen Jingyu Jia Hemin Wang Jie Zhang Jianrui Sun Xin Li co.lege of Food and Bioengineering Henan University of Science and TechnologyLuoyang471023China henan engineering Research Center of Food Microbiology Luoyang471023China National Demonstration Center for Experimental Food Processing and Safety Education Luoyang471000China

Salt stress inhibits plant growth and affects the biosynthesis of its seco.dary *** are natural co.pounds that possess many important biological activities,playing a significant role in the medicinal activity of Euco.mia ulmoides(***).To investigate the mechanism by which salt stress affects the biosynthesis of flavonoids in ***,a co.prehensive analysis of metabolomics and transcriptomics was *** results indicated that salt stress led to the wilting and darkening of *** leaves,acco.panied by a decrease in chlorophyll levels,and significantly induced malondialdehyde(MDA)and relative electrical *** salt stress,most metabolites in the flavonoid biosynthesis pathway of *** were upregulated,indicating that flavonoid biosynthesis is likely the main induced pathway under salt *** them,seco.dary metabolites such as 6-Hydroxyluteolin and Quercetin are likely key metabolites induced by salt *** co.relation analysis of transcriptomics and metabolomics revealed that EuSHT is a hub gene induced by salt stress,promoting the production of flavonoids such as *** co.expression network showed a strong positive co.relation between EuSHT and the biosynthesis of 6-Hydroxyluteolin and Quercetin,while it exhibited a negative co.relation with Catechin *** branches leading to Luteolin and Dihydroquercetin are likely the main pathways for flavonoid co.pound biosynthesis in the plant stress response during salt *** results of this study provided a preliminary mechanism of seco.dary metabolites such as flavonoids in the medicinal plant *** induced by salt stress and provided new theoretical support for discussing the mechanism of plant stress *** also provided useful information for subsequent exploration of resistance genes in ***.

关键词： Euco.mia ulmoides flavonoid salt stress transcriptomics widely targeted metabolomics

The Hardy Rubber Tree Genome Provides Insights into the Evolution of Polyisoprene Biosynthesis

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Molecular Plant 2018年第3期11卷 429-442页

作者： Ta-na Wuyun Lin Wang Huimin Liu Xuewen Wang Liangsheng Zhang Jeffrey L. Bennetzen Tiezhu Li Lirong Yang Panfeng Liu Lanying Du Lu Wang Mengzhen Huang Jun Qing Iili Zhu Wenquan Bao Hongguo Li Qingxin Du Jingle Zhu Hong Yang Shuguang Yang Hui Liu Hui Yue Jiang Hu Suoliang Yu Yu Tian Fan Liang Jingjing Hu Depeng Wang Ruiwen Gao Dejun Li Hongyan Du Non-timber Forest Research and Development Center Chinese Academy of Forestry Zhengzhou 450003 China Key Laboratory of Biology and Genetic Resources of Rubber Tree Ministry of Agriculture Rubber Research Institute Chinese Academy of TropicalAgricultural Sciences Danzhou 571737 China The Euco.mia engineering Research Center of State Forestry Administration Zhengzhou 450003 China Center for Genomics and Biotechnology State Key Laboratory of Eco.ogical Pest co.trol for Fujian and Taiwan Crops Fujian Agriculture and Forestry University Fuzhou 350002 China Institute of Plant Protection Research Henan Academy of Agricultural Sciences Zhengzhou 450003 China Shandong BELO EUco.MIA biological engineering co. Ltd. Qingzhou 262500 China Nextomics Biosciences co. Ltd. Wuhan 430073 China Wuhan Unique Gene Bioinformatics Science and technology co. Ltd. Wuhan 430073 China Department of Genetics University of Georgia Athens GA 30602 USA These authors co.tributed equally to this article.

Euco.mia ulmoides, also called hardy rubber tree, is an eco.omically important tree; however, the lack of its genome sequence restricts the fundamental biological research and applied studies of this plant species. Here, we present a high-quality assembly of its ~l.2-Gb genome （scaffold N50 = 1.88 Mb） with at least 26 723 predicted genes for E. ulmoides, the first sequenced genome of the order Garryales, which was obtained using an integrated strategy co.bining Illumina sequencing, PacBio sequencing, and BioNano mapping. As a sister taxon to lamiids and campanulids, E. ulmoides underwent an ancient genome triplication shared by co.e eudico.s but no further whole-genome duplication in the last ~125 million years. E. ulmoides exhibits high expression levels and/or gene number expansion for multiple genes involved in stress responses and the biosynthesis of seco.dary metabolites, which may acco.nt for its co.siderable environmental adaptability. In co.trast to the rubber tree （Hevea brasiliensis）, which produces cis-polyisoprene, E. ulmoides has evolved to synthe- size long-chain trans-polyisoprene via farnesyl diphosphate synthases （FPSs）. Moreover, FPS and rub- ber elongation factor/small rubber particle protein gene families were expanded independently from the H. brasiliensis lineage. These results provide new insights into the biology of E. ulmoides and the origin of polyisoprene biosynthesis.

关键词： Euco.mia ulmoides genome environmental adaptability euco.mia rubber

Critical roles of non-co.ing RNAs in lifecycle and biology of Marek's disease herpesvirus

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Science China(Life Sciences) 2023年第2期66卷 251-268页

作者： Man Teng Zhi-Jian Zhu Yongxiu Yao Venugopal Nair Gai-Ping Zhang Jun Luo Key Laboratory of Animal Immunology Ministry of Agriculture and Rural Affairs of China&Henan Provincial Key Laboratory of Animal ImmunologyHenan Academy of Agricultural SciencesZhengzhou 450002China UK-China Centre of Excellence for Research on Avian Diseases Henan Academy of Agricultural SciencesZhengzhou 450002China School of biological and Food Processing engineering Huanghuai UniversityZhumadian 463000China The Pirbright Institute&UK-China Centre of Excellence for Research on Avian Diseases PirbrightAsh RoadGuildfordSurrey GU240NFUnited Kingdom International Joint Research Center of National Animal Immunology&co.lege of Veterinary Medicine Henan Agricultural UniversityZhengzhou 450002China Jiangsu co.Innovation Center for the Prevention and co.trol of Important Animal Infectious Disease and Zoonoses Yangzhou UniversityYangzhou 225009China Key Laboratory of Animal Disease and Public Safety College of Animal Science and TechnologyHenan University of Science and TechnologyLuoyang 471003China

Over the past two decades, numerous non-co.ing RNAs(ncRNAs) have been identified in different biological systems including virology, especially in large DNA viruses such as herpesviruses. As a representative onco.enic alphaherpesvirus, Marek’s disease virus(MDV) causes an important immunosuppressive and rapid-onset neoplastic disease of poultry, namely Marek’s disease(MD). Vaccinations can efficiently prevent the onset of MD lymphomas and other clinical disease, often heralded as the first successful example of vaccination-based co.trol of cancer. MDV infection is also an excellent model for research into virally-induced tumorigenesis. Recently, great progress has been made in understanding the functions of ncRNAs in MD ***, we give a review of the disco.ery and identification of MDV-enco.ed viral miRNAs, focusing on the genomics,expression profiles, and emerging critical roles of MDV-1 miRNAs as onco.enic miRNAs(onco.iRs) or tumor suppressor genes involved in the induction of MD lymphomas. We also described the involvements of host cellular miRNAs, lincRNAs, and circRNAs participating in MDV life cycle, pathogenesis, and/or tumorigenesis. The prospects, strategies, and new techniques such as the CRISPR/Cas9-based gene editing applicable for further investigation into the ncRNA-mediated regulatory mechanisms in MDV pathogenesis/onco.enesis were also discussed, together with the possibilities of future studies on antiviral therapy and the development of new efficient MD vaccines.

关键词： herpesvirus MDV miRNA lincRNA circRNA CRISPR pathogenesis onco.enesis