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Journal of Integrative Plant Biology 2014年第5期56卷 492-504页

作者： Bangwei Zhou álvaro Sanz-Sáez Abdelhalim Elazab tianmin Shen Rut Sánchez-Bragado Jordi Bort Maria Dolors Serret José Luis Araus Unit of Plant Physiology Faculty of Biology University of Barcelona henan tianmin seed company Lankao County Department of Plant Biology University of Illinois Urbana-Champaign

This experiment aims to test the traits responsible for the increase in yield potential of winter wheat released in henan Province, China. Seven established cultivars released in the last 20 years and three advanced lines were assayed. The results showed that grain yield was positively correlated with harvest index（HI）, kernel number per square meter, and aboveground biomass. In addition, the HI and aboveground biomass showed an increasing trend with the year of ***, we can conclude that bread wheat breeding advances during recent decades in henan Province, China,have been achieved through an increase in HI, kernel number per square meter, and aboveground biomass. A higher d13C seems also to be involved in these advances, which suggests a progressive improvement in constitutive water use efficiency not associated with a trend towards lower stomatal conductance in the most recent genotypes. However, genetic advance Researchdoes not appear related to changes in photosynthesis rates on area basis when measured in the flag leaf or the spike,but only to a higher, whole‐spike photosynthesis. Results also indirectly support the concept that under potential yield conditions, the spike contributed more than the flag leaf to kernel formation.

关键词： Carbon isotopes grain yield net carbon exchange nitrogen content yield potential

PREDICTING HETEROSIS IN GRAIN YIELD FROM GENETIC DIVERGENCE ANALYSIS IN SUNFLOWER

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生物数学学报 1995年第4期10卷 19-27页

作者： QiaoChungui SunYushu LiDianshen SunChangzhan LiChunzi LiShuqiang Jilin Agricultural University Changchun 130118 Jilin Provincial seed company Changchu

Geneticdivergenceanalysiswasconductedbasedonperformanceof12quantitativetraitsin85sunflowerinbredlines,tostudyi***enheterosisofseedoilcontentandgeneticdistance,andquadraticregressionequationtherelationshipbetweengrainy... 详细信息

关键词： Sunflower Geneticdivergence Clusteranalysis Heterosis

Development of an STS Marker Linked to Powdery Mildew Resistance Genes PmLK906 and Pm4a by Gene Chip Hybridization

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Agricultural Sciences in China 2010年第3期9卷 331-336页

作者： NIU Ji-shan JIA Hai-yan YIN Jun WANG Bao-qin MA Zheng-qiang SHEN Tian-min National Centre of Engineering and Technological Research for Wheat/henan Agricultural University Zhengzhou 450002 P.R.China State Key Laboratory of Crop Genetics and Germplasm Enhancement/Nanjing Agricultural University Nanjing 210095 P.R.China Tian-Min seed Limited company Lankao 730070 P.R.China

Wheat （Triticum aestivum L.） line Lankao 90（6） carries a recessive powdery mildew resistance gene temporarily named PmLK906 on chromosome 2AL. Near PmLK906 there is another known powdery mildew resistance gene locus Pm4. To track the two powdery mildew resistance genes in wheat breeding program by marker assisted selection （MAS）, a linked molecular marker was developed in this study. Wheat gene chip hybridization combined with bulked segregant analysis （BSA） was used to develop an sequence-tagged sites （STS） marker for PmLK906 and Pm4. A new 2 125 bp full-length cDNA clone （GenBank accession no. EU082094） similar to csAtPR5 ofAegilops tauschii was isolated from Lankao 90（6） 21-12, and temporarily named TaAetPR5. Specific products could be amplified from cultivars or lines possessing Pm4a, Pm4b and PmLK906 with primers p9-7pl and p9-7p2 derived from TaAetPR5. TaAetPR5 was linked to PmLK906 at a genetic distance of 7.62 cM, and cosegregated with Pm4a. The p9-7p1 and p9-7p2 could be used as an STS marker for these resistance genes in wheat breeding. Because this marker was cosegregated with Pm4a, it can be used in map-based cloning of the alleles at Pm4 locus also.

关键词： wheat powdery mildew TaAetPR5 sequence-tagged sites (STS) molecular marker

Strategies in American Alfalfa Breeding

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Strategies in American Alfalfa Breeding

Screening of Low Tar Flue Cured Tobacco Varieties

第五届中国苜蓿发展大会

作者： Mike Velde Zixue Liu Liming Hou Dairyland seed Co. Beijing Clover seed&turf Co. Global seed company

"牧草之王"苜蓿可以为奶牛和肉牛提供足够的能量和蛋白质,而被认为是人类食物生产的推动者,应用越来越广泛。本文以苜蓿产业发展先进的美国为例,讲述了美国苜蓿品种的选育、新的育种方法的应用(基因工程分子育种等)、新的苜蓿育种方向...

"牧草之王"苜蓿可以为奶牛和肉牛提供足够的能量和蛋白质,而被认为是人类食物生产的推动者,应用越来越广泛。本文以苜蓿产业发展先进的美国为例,讲述了美国苜蓿品种的选育、新的育种方法的应用(基因工程分子育种等)、新的苜蓿育种方向以及对苜蓿育种进行了展望。用途更广泛抗逆性更强的苜蓿新品种的不断选育和推广,为苜蓿产业的快速发展奠定了坚实的基础。

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Asian Agricultural Research 2017年第11期9卷 66-69页

作者： Yanji JIN Hua CUI Shuhua LIN Guohe WU Lijuan SUN Chong GAO Chengrong AN Yanbian Agricultural Science Research Institution Zhenlai seed company of Jilin Province

The main agronomic traits,economic traits,appearance quality,main chemical components,gas composition and physical parameters of 10 flue cured tobacco varieties were investigated in the *** the field natural incidence of the main diseases was *** results showed that main agronomic characters of the 10 varieties were in line with the breeding objectives,among which 5 varieties have good economic characters;5 varieties have good appearance quality;6 varieties have coordinated chemical composition and high potassium content;9 varieties have good smoke components and physical indicators.

关键词： Flue cured tobacco Varieties Low tar Screening

The Optimum Sowing Time for Plastic-film Corn and the Application of Two Related Theories

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Agricultural Sciences in China 2002年第5期1卷 508-515页

作者： WU Rui-xiang, LIU Rong-quan, LU Cui-ling, LU Yong-lai, LI Hua, ZHANG Li, LU Xiu-zhi, YU Shu-ping, WU Xiu-yan and WANG Xin-huan(Ningcheng Agro-technique Extension Centre , Inner Mongolia Autonomous Region , Tianyi 024200 , P. R. China Ningcheng seed company , Inner Mongolia Autonomous Region , Tianyi 024200 , P. R . China) Ningcheng Agro-technique Extension Centre Inner Mongolia Autonomous Region Tianyi 024200 P. R. China Ningcheng seed company Inner Mongolia Autonomous Region Tianyi 024200 P. R. China

There are two main theories, the 'temperature-raising' theory and the 'precipitation-based regulation' theory, which guide the optimum sowing time of the plastic-film corn. The former was applied in the humid or semi-humid ecotope and on irrigated or half-shaded land in the arid and semi-arid ecotopes, while the latter was suitable for the dry-farming land in the semi-arid ecotope. The results of experiments and investigations for many years showed that the corn output was increased by 69.2% when the former theory was applied to guide the optimum sowing time for plastic-film corn in the semi-humid ecotope, and by 60. 0% when the latter theory was applied in the semi-arid ecotope. In the semi-arid ecotope, however, the output was increased only by 15.7% when the former theory was applied, and even dropped by 14.4% when the latter theory was applied.

关键词： Plastic-film corn Division of climatic ecology Optimum sowing time 'Temperature-raising' theory 'Precipitation-based regulation' theory

Self S‑RNase reduces the expression of two pollen‑specific COBRA genes to inhibit pollen tube growth in pear

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Molecular Horticulture 2023年第1期3卷 39-50页

作者： Lei Wu Ying Xu Kaijie Qi Xueting Jiang Min He Yanbo Cui Jianping Bao Chao Gu Shaoling Zhang Centre of Pear Engineering Technology Research State Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjingJiangsuChina College of Life Sciences Nanjing Agricultural UniversityNanjingJiangsuChina Nanjing Ningcui Biological seed company Limited NanjingJiangsuChina College of Plant Science Tarim UniversityAlaerXinjiang 843300China

Due to self-incompatibility(SI)prevents self-fertilization,natural or artificial cross-pollination has been conducted in many orchards to stabilize fruit ***,it is still puzzled which routes of self S-RNase arresting pollen tube ***,17 COBRA genes were isolated from pear *** these genes,the pollen-specifically expressed PbCOB.A.1 and PbCOB.A.2 positively mediates pollen tube *** promoters of PbCOB.A.1 and/or PbCOB.A.2 were bound and activated by PbABF.E.2(an ABRE-binding factor)and PbC2H2.K16.2(a C2H2-type zinc finger protein).Notably,the expressions of PbCOB.A.1,PbCOB.A.2,and PbC2H2.K16.2 were repressed by self S-RNase,suggesting that self S-RNase reduces the expression of PbCOB.A.1 and PbCOB.A.2 by decreasing the expression of their upstream factors,such as PbC2H2.K16.2,to arrest pollen tube ***.A.1 or PbCOB.A.2 accelerates the growth of pollen tubes treated by self S-RNase,but can hardly affect level of reactive oxygen species and deploymerization of actin cytoskeleton in pollen tubes and cannot physically interact with any reported proteins involved in *** results indicate that PbCOB.A.1 and PbCOB.A.2 may not relieve S-RNase toxicity in incompatible pollen *** information provides a new route to elucidate the arresting pollen tube growth during SI reaction.

关键词： Pyrus Self-incompatibility COBRA C2H2-type zinc finger protein Pollen tube growth S-RNase

Effects of Artificial Aging on Physiological Characteristics of Rice seeds with Different Dormancy Characteristics

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Agricultural Biotechnology 2019年第1期8卷 52-56页

作者： Dongdong CAO Shanyu CHEN Yutao HUANG Yebo QIN Guanhai RUAN Institute of Crop and Nuclear Technology Utilization Zhejiang Academy of Agricultural Science Huzhou Keao seed company Co.Ltd Zhejiang Planting Administration Bureau

In this study,two indica varieties with different dormancy characteristics [4 K58( II-32 B dormant),4 K59( II-32 B) ] and their F2 seeds( C178,C179) obtained through hybridization with sterile line( II-32 A) were used as materials. Different aged seeds( 0,3,6 and 9 d) of these four varieties were acquired by artificial accelerated aging method. Effects of artificial aging on malondialdehyde( MDA) content and catalase( CAT),ascorbate peroxidase( APX),peroxidase( POD) and superoxide dismutase( SOD) activity were investigated. The results showed that with the prolongation of aging time,MDA contents of these four rice varieties increased significantly,while the activity of antioxidant enzymes decreased dramatically. Besides,MDA contents and SOD,CAT and APX activity of the two inbred lines were significantly lower than those of the hybrid varieties. In addition,the activity of antioxidant enzymes in 4 K58 and C178 was significantly lower than that in 4 K59 and C179,respectively. This study indicated that artificial aging treatment significantly inhibited the activity of antioxidant system in seeds,improved membrane lipid peroxidation degree,and thus aggravated the deterioration of seeds. In addition,it also suggested that rice seeds with dormant property were more intolerant to storage.

关键词： Artificial accelerated aging Rice seed Physiological characteristics Dormancy

The telomere-to-telomere genome of Fragaria vesca reveals the genomic evolution of Fragaria and the origin of cultivated octoploid strawberry

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Horticulture Research 2023年第4期10卷 101-109页

作者： Yuhan Zhou Jinsong Xiong Ziqiang Shu Chao Dong Tingting Gu Pengchuan Sun Shuang He Mian Jiang Zhiqiang Xia Jiayu Xue Wasi Ullah Khan Fei Chen Zong-Ming Cheng College of Horticulture Nanjing Agricultural UniversityNanjing 210095China Hainan Yazhou Bay seed Laboratory Sanya 572024China Wuhan Benagen Tech Solutions company Limited WuhanHubei 430021China Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education&State Key Laboratory of Hydraulics&Mountain River Engineering College of Life SciencesSichuan UniversityChengdu 610065China College of Tropical Crops Hainan UniversityHaikou 570228China Sanya Nanfan Research Institute from Hainan University Sanya 572025China

Fragaria vesca,commonly known as wild or woodland strawberry,is the most widely distributed diploid Fragaria species and is native to Europe and *** of its small plant size,low heterozygosity,and relative ease of genetic transformation,*** has been a model plant for fruit research since the publication of its Illumina-based genome in ***,its genomic contribution to octoploid cultivated strawberry remains a long-standing ***,we de novo assembled and annotated a telomere-to-telomere,gap-free genome of ***‘Hawaii 4’,with all seven chromosomes assembled into single contigs,providing the highest completeness and assembly quality to *** gap-free genome is 220785082 bp in length and encodes 36173 protein-coding gene models,including 1153 newly annotated *** 14 telomeres and seven centromeres were annotated within the seven *** the three previously recognized wild diploid strawberry ancestors,***,***,and ***,phylogenomic analysis showed that *** and *** are the ancestors of the cultivated octoploid strawberry F.×ananassa,and *** is its closest *** subgenomes of F.×ananassa belong to the *** group,and one is sister to *** anticipate that this high-quality,telomere-to-telomere,gap-free *** genome,combined with our phylogenomic inference of the origin of cultivated strawberry,will provide insight into the genomic evolution of Fragaria and facilitate strawberry genetics and molecular breeding.

关键词： straw Fragaria origin

Efficient DIPA-CRISPR-mediated knockout of an eye pigment gene in the white-backed planthopper,Sogatella furcifera

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Insect Science 2024年第4期31卷 1015-1025页

作者： Meng-Qi Zhang Lang-Lang Gong Ya-Qin Zhao Yun-Feng Ma Gui-Jun Long Huan Guo Xuan-Zheng Liu J.Joe Hull Youssef Dewer Chao Yang Ning-Ning Zhang Ming He Peng He National Key Laboratory of Green Pesticides Key Laboratory of Green Pesticide and Agricultural BioengineeringMinistry of EducationCenter for R&D of Fine Chemicals of Guizhou UniversityGuiyangChina Pest Management and Biocontrol Research Unit US Arid Land Agricultural Research CenterUSDA Agricultural Research ServicesMaricopaArizonaUSA Phytotoxicity Research Department Central Agricultural Pesticide LaboratoryAgricultural Research CenterDokkiGizaEgypt Guizhou Jifeng seed Industry Limited Liability company XingyiGuizhou ProvinceChina Shandong Facility Horticulture Bioengineering Research Center Weifang University of Science and TechnologyWeifangShandong ProvinceChina

Although CRISPR/Cas9 has been widely used in insect gene editing,the need for the microinjection of preblastoderm embryos can preclude the technique being used in insect species with eggs that are small,have hard shells,and/or are difficult to collect and maintain outside of their normal *** is the case with Sogatella furcifera,the white-backed planthopper(WBPH),a significant pest of Oryza sativa(rice)that oviposits inside rice *** extraction from the stem runs the risk of mechanical damage and hatching is heavily influenced by the micro-environment of the rice *** bypass these issues,we targeted embryos prior to oviposition via direct parental(DIPA)-CRISPR,in which Cas9 and single-guide RNAs(sgRNAs)for the WBPH eye pigment gene tryptophan 2,3-dioxygenase were injected into the hemocoel of adult *** at varying numbers of days posteclosion were evaluated to determine at what stage their oocyte might be most capable of taking up the gene-editing *** evaluation of the offspring indicated that the highest G0 gene-edited efficacy(56.7%)occurred in females injected 2 d posteclosion,and that those mutations were heritably transmitted to the G1 *** study demonstrates the potential utility of DIPA-CRISPR for future gene-editing studies in non-model insect species and can facilitate the development of novel pest management applications.

关键词： CRISPR/Cas9 DIPA-CRISPR gene editing tryptophan 2,3-dioxygenase WBPH