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Gas stunning with CO_2 affected meat color,lipid peroxidation, oxidative stress, and gene expression of mitogen-activated protein kinases, glutathione S-transferases,and Cu/Zn-superoxide dismutase in the skeletal muscles of broilers

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Journal of Animal Science and Biotechnology 2018年第3期9卷 738-749页

作者： Lei Xu Haijun Zhang Hongyuan Yue Shugeng Wu Haiming Yang Zhiyue Wang Guanghai Qi Key Laboratory of feed Biotechnology of Ministry of Agriculture & National Engineering Research Center of Biological Feed Feed Research Institute Chinese Academy of Agricultural Sciences College of Animal Science and technology Yangzhou University

Background: Meat color and lipid peroxidation are important traits related to meat quality. CO_2 concentration is a critical factor that can affect meat quality in the commercial use of gas stunning(GS). However, the effect and mechanism of CO_2 stunning on meat color and lipid peroxidation during long-term storage remain poorly studied. We aimed to study the effects of GS methods, especial y CO_2 concentration, on meat color and meat lipid peroxidation in broilers during long-term storage at 4 °C and to explore the potential mechanism of meat color change via lipid peroxidation and the inner lipid peroxide scavenging ***: Eighteen broilers were sacrificed after exposure to one of the following gas mixtures for 90 s: 40% CO_2+21% O_2+ 39% N2(G40%), 79% CO_2+ 21% O_2(G79%), or no stunning(0% CO_2, control). Meat color, serum variables,enzyme activities, and the gene expression of mitogen-activated protein kinase(MAPK), nuclear factor-erythroid2-related factor 2(Nrf2), glutathione S-transferase(GST) and superoxide dismutase(SOD) were determined.(Continued on next page)Results: The concentrations of serum triiodothyronine(T3, P = 0.03) and the ratio of serum free triiodothyronine/free thyroxine(FT3/FT4, P < 0.01) were decreased, whereas levels of serum cortisol(P < 0.01) were increased in the 40%CO_2 group compared with the control group. Additionally, the thiobarbituric acid-reactive substances(TBARS)3 d(P < 0.01) and TBARS6 d(P = 0.01) in breast meat and the TBARS3 din thigh meat(P < 0.01) were increased in the40% CO_2 group compared with the control group. Serum T3 was negatively correlated with TBARS6 dboth in the breast and thigh meat(r =-0.63, P < 0.01 and r =-0.47, P = 0.05 respectively). T3/T4 was negatively correlated with TBARS6 din the breast meat and in the thigh meat(r =-0.57, P = 0.01; and r =-0.53, P = 0.03 respectively). Compared with the control group, Lightness(L*)1 d(P = 0.03) and L*9 d(P < 0.01) were increased, whereas total chromatic abe

关键词： Antioxidant enzyme Broiler Controlled atmosphere stunning Gas stunning Lipid peroxidation MAPK/Nrf2/ARE Meat color Oxidative stress

Sources,dynamics in vivo,and application of astaxanthin and lutein in laying hens:A review

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Animal Nutrition 2023年第2期13卷 324-333页

作者： Hanyi Shi Xuejuan Deng Xiaoyu Ji Ning Liu Huiyi Cai Department of Animal Science Henan University of Science and TechnologyLuoyang471023HenanChina Department of Poultry Science University of GeorgiaAthens30602GAUSA National engineering research center of Biological feed Beijing100081China

Astaxanthin(Ax)and lutein are important fat-soluble pigments and essential nutrients for human and animal *** pluvialis microalga and Phaffia rhodozyma yeast are ideal species for commercial Ax *** flowers are a main source of commercial *** of dietary Ax and lutein in the gastrointestinal tract are similar to lipids,but their activities are tremendously challenged by many physiological and dietary factors;few data are available about these in *** Ax and lutein have insignificant effects on egg production and egg physical properties,but have pronounced effects on yolk color,nutrition,and *** two pigments can also enhance antioxidative capacity and immune function of laying hens.A few studies have shown that Ax and lutein can improve fertilization and hatchability of laying *** the pigmentation and health benefits of Ax and lutein from hen feed to human food,the commercial availability,chicken yolk improvement,and immune function of Ax and lutein are the focuses of this *** potential roles of carotenoids in the cytokine storm and gut microbiota are also briefly *** bioavailability,metabolism,and deposition of Ax and lutein in laying hens are suggested for future research.

关键词： Application Astaxanthin Egg yolk Food chain Laying hen Lutein

Effects of β-TCP Ceramics on Osteoblast Cellular Proliferating,Mineralization and Osteocalcin Expression

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Journal of Wuhan University of technology(Materials Science) 2012年第1期27卷 107-109页

作者： QI Zhitao ZHANG Qihuan ZHENG Qiang DAI Honglian WANG Zisheng QIU Ming LI Shipu School of Chemical and Biological engineering Yancheng Institute of Technology The Bio-material research and engineering center Wuhan University of Technology Yancheng Tianbang feed technology Co.Ltd

After co-cultrured osteoblast with fl-TCP ceramics, the cellular proliferating, mineralization and osteocalcin expression were studied. MTT assay showed that fl-TCP ceramics had no affect on cellular proliferating. Laser scanning confocal detection showed that fl-TCP ceramics could increase the mineralization level of osteoblast. Furthermore, RT-PCR showed that fl-TCP could increase the expression level of osteocalcin. Those results indicate β-TCP ceramics had perfect biocompatibility and increased the mineralization of osteoblast to accelerate osteogenesis by means of affecting the expression of genes involving in osteogeneticprocess.

关键词： β-TCP ceramics osteoblast cellular proliferating mineralization osteocalcin

Lactiplantibacillus plantarum FRT4 attenuates high-energy low-protein dietinduced fatty liver hemorrhage syndrome in laying hens through regulating gut-liver axis

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Journal of Animal Science and Biotechnology 2024年第3期15卷 1292-1311页

作者： Daojie Li Hongying Cai Guohua Liu Yunsheng Han Kai Qiu Weiwei Liu Kun Meng Peilong Yang Key Laboratory of feed Biotechnology of Ministry of Agriculture and Rural Affairs Institute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China National engineering research center of Biological feed Beijing 100081China

Background Fatty liver hemorrhage syndrome(FLHS)becomes one of the most major factors resulting in the laying hen death for caged egg *** study aimed to investigate the therapeutic effects of Lactiplantibacillus plan-tarum(***)FRT4 on FLHS model in laying hen with a focus on liver lipid metabolism,and gut *** The FLHS model of laying hens was established by feeding a high-energy low-protein(HELP)diet,and the treatment groups were fed a HELP diet supplemented with differential proportions of *** *** results indicated that *** FRT4 increased laying rate,and reduced the liver lipid accumulation by regulating lipid metabolism(lipid synthesis and transport)and improving the gut microbiota ***,***-tarum FRT4 regulated the liver glycerophospholipid ***,“gut-liver”axis analysis showed that there was a correlation between gut microbiota and lipid *** The results indicated that *** FRT4 improved the laying performance and alleviated FLHS in HELP diet-induced laying hens through regulating“gut-liver”*** findings reveal that glycerophospholipid metabolism could be the underlying mechanism for the anti-FLHS effect of *** FRT4 and for future use of *** FRT4 as an excellent additive for the prevention and mitigation of FLHS in laying hens.

关键词： Fatty liver hemorrhage syndrome Gut microbiota Lactiplantibacillus plantarum FRT4 Laying hens Lipid metabolism

Nitric Oxide and Brassinosteroids Mediated Fungal Endophyte-Induced Volatile Oil Production Through Protein Phosphorylation Pathways in Atractylodes lancea Plantlets

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Journal of Integrative Plant Biology 2013年第11期55卷 1136-1146页

作者： Cheng-Gang Ren Chuan-Chao Dai Jiangsu Key Laboratory for microbes and Functional Genomics Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources College of Life Sciences in Nanjing Normal University

Fungal endophytes have been isolated from almost every plant, infecting their hosts without causing visible disease symptoms, and yet have still proved to be involved in plant secondary metabolites accumulation. To decipher the possible physiological mechanisms of the endophytic fungus-host interaction, the role of protein phosphorylation and the relationship between endophytic fungus-induced kinase activity and nitric oxide （NO） and brassinolide （BL） in endophyte-enhanced volatile oil accumulation in Atractylodes lancea plantlets were investigated using pharmacological and biochemical approaches. Inoculation with the endophytic fungus Gilmaniella sp. ALl2 enhanced the activities of total protein phosphorylation, Ca2^-dependent protein kinase, and volatile oil accumulation in A. lancea plantlets. The upregulation of protein kinase activity could be blocked by the BL inhibitor brassinazole. Furthermore, pretreatments with the NO-specific scavenger cPTIO significantly reduced the increased activities of protein kinases in A. lancea plantlets inoculated with endophytic fungus. Pretreatments with different protein kinase inhibitors also reduced fungus-induced NO production and volatile oil accumulation, but had barely no effect on the BL level. These data suggest that protein phosphorylation is required for endophyte- induced volatile oil production in A. lancea plantlets, and that crosstalk between protein phosphorylation and the NO pathway may occur and act as a downstream signaling event of the BL pathway.

关键词： Atractylodes lancea endophytic fungus protein phosphorylation signal molecule volatile oi1.

Fermented soybean meal improved laying performance and egg quality of laying hens by modulating cecal microbiota,nutrient digestibility,intestinal health,antioxidant and immunological functions

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Animal Nutrition 2024年第3期18卷 309-321页

作者： Uchechukwu Edna Obianwuna Lingling Huang Haijun Zhang Jing Wang Guanghai Qi Kai Qiu Shugeng Wu National engineering research center of Biological feed Institute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China Wilmar(Shanghai)Biotechnology research&Development center Co. Ltd.Shanghai 200137China

Antinutritional factors in feedstuffs may limit their utilization in livestock production,but fermentation process can be used to improve feed quality;however,studies on fermented soybeans for laying hens remain *** investigated the effect of fermented soybean meal(FSBM)at various inclusion levels as a partial replacement for soybean meal(SBM)on egg production,egg quality,amino acid digestibility,gut morphology and microbiota,antioxidant capacity and immune response of young laying hens.A total of 360 Hy-line Brown laying hens aged 18 weeks were selected and divided into 5 groups of 6 replicates each and 12 birds per *** control group received a basal diet while the trial group received the basal diet with FSBM included at 2.5%,5.0%,7.5%and 10.0%,respectively,for 12 *** findings revealed that the nutritional value of FSBM was higher compared to that of SBM in terms of reduced content of trypsin inhibitors and increased contents of crude protein,amino acids and *** enhanced egg production(Pfeed-to-egg ratio(P<0.05),and albumen quality(albumen height and Haugh unit)(P<0.05).Furthermore,FSBM improved apparent fecal amino acid digestibility(P<0.05),gut morphology(increased villus height,villus width,villus height-to-crypt depth ratio and decreased crypt depth)(P<0.05),antioxidant capacity(reduced malondialdehyde and increased catalase,total superoxide dismutase,glutathione peroxidase and total antioxidant capacity)(P<0.05)and immune function(increased concentrations of IgG,IgA,and IgM;increased levels of transforming growth factor beta and Toll-like receptor 2;and reduced levels of interleukin 1βand tumor necrosis factor alpha)(P<0.05).Further analysis showed that FSBM altered the composition of the gut microbiota favoring beneficial *** findings suggest that probiotic fermentation improved the nutritional value of *** inclusion of FSBM in the diets of laying hens at 2.5%or 5.0%improved amino acid digestibility,gut

关键词： Fermented soybean meal Laying hen Egg production Egg quality Immune response

Dietary supplemental xylooligosaccharide modulates nutrient digestibility,intestinal morphology,and gut microbiota in laying hens

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Animal Nutrition 2021年第1期7卷 152-162页

作者： Jianmin Zhou Shugeng Wu Guanghai Qi Yu Fu Weiwei Wang Haijun Zhang Jing Wang Laboratory of Quality&Safety Risk Assessment for Animal Products on feed Hazards(Beijing)of the Ministry of Agriculture&Rural Affairs and National Engineering Research Center of Biological FeedFeed Research InstituteChinese Academy of Agricultural SciencesBeijing100081China

This study was conducted to evaluate the prebiotic effects of dietary xylooligosaccharide(XOS)sup-plementation on performance,nutrient digestibility,intestinal morphology,and gut microbiota in laying *** a 12-wk experiment,a total of 288 Hy-Line Brown layers at 50 wk of age were randomly assigned into 3 dietary treatments supplemented with XOS at 0,200 or 400 mg/*** treatment had 8 replicates with 12 birds *** fed XOS diets showed a lower feed-to-egg ratio during wk 7 to 12 and a higher egg yolk color value in wk 12 compared with those fed the control diet(Pfeed conversion ratio by modulating nutrient digestibility and ileal morphology in laying hens,which could be attributed to the enhancement of bacterial diversity and alteration of microbial composition.

关键词： Gut microbiota Ileal morphology Laying hen Nutrient digestibility Xylooligosaccharide

Solubilization of potassium containing minerals by high temperature resistant Streptomyces *** from earthworm's gut

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Acta Geochimica 2016年第3期35卷 262-270页

作者： Dian Feng Liu Bin Lian Bin Wang Jiangsu Key Laboratory for microbes and Functional Genomics Jiangsu Engineering and Technology Research Center for Microbiology College of Life Sciences Nanjing Normal University No. 1 Wenyuan Road Department of Bioengineering Puyang Vocational & Technical Institute State Key Laboratory of Environmental Geochemistry Institute of Geochemistry Chinese Academy of Sciences Department of Bio-engineering Henan Institute of Science and Technology

A potassium solubilizing bacterial strain des- ignated EGT, which is tolerant of high temperature, was isolated from an earthworm＇s gut to obtain a bacterium that can weather potassium-bearing rock effectively through solid-state fermentation. Molecular phylogeny and 16S rRNA gene sequence analysis demonstrated the bacterial strain was a member of the Streptomyces genus. To assess its potential to release potassium from silicate minerals, this strain was used to degrade potassium-bearing rock powder by solid-state fermentation. After fermentation, the amount of water-soluble A1, Fe and K of the substrate with active inoculum was higher than those of the control, which had autoclaved inoculum, and those of the fresh substrate. The result indicated that the strain had the ability to weather potassium-bearing rock and could be used as an inoculum in the production of potassium bio-fertilizer, due to its potassium release activity from rock and tolerance to high temperature.

关键词： Weathering Potassium solubilizing bacteria Streptomyces

Soybeanβ-conglycinin and glycinin reduced growth performance and the intestinal immune defense and altered microbiome in juvenile pearl gentian groupers Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂

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Animal Nutrition 2022年第2期9卷 193-203页

作者： Yuanfa He Jinfang Liang Xiaohui Dong Hongyu Liu Qihui Yang Shuang Zhang Shuyan Chi Beiping Tan Laboratory of Aquatic Animal Nutrition and feed Fisheries CollegeGuangdong Ocean UniversityZhanjiang 524088China Aquatic Animals Precision Nutrition and High-Efficiency feed engineering research center of Guangdong Province Zhanjiang 524088China Key Laboratory of Aquatic Livestock and Poultry Feed Science and Technology in South ChinaMinistry of AgricultureZhanjiang 524088China

The utilization efficiency of soy protein is affected by its 2 anti-nutritional substancesdthe antigens bconglycinin and *** study investigated their effects on the growth performance,intestinal immune defense,andmicrobiome in juvenile pearl gentian groupers(Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂).Three isonitrogenous and isolipidic diets were formulated containing fishmeal supplemented with 70 g/kgβ-conglycinin or 100 g/kg glycinin,or no supplementation(control).Each experimental diet was fed to quadruplicate groups with 30 fish in each tank for 8 *** inclusion of eitherβ-conglycinin or glycinin significantly reduced weight gain and specific growth rates,and cell proliferation of the distal *** evaluation of the intestine tract revealed the inflammation signs,characterized by reducing of plica height and width as well as the number of the goblet cells,and widening of the lamina *** group fed theβ-conglycinin diet had reduced lysozyme activity,contents of immunoglobulin M and complements 3 and *** activities of caspase-3 and-9 were observed in the group fed the bconglycinin diet compared to the other 2 *** the intestinal microbiota,the relative abundances of the potentially pathogenic genera Photobacterium and Vibrio were significantly higher in the glycinin group than those in ***,the existence of soybean antigens(β-conglycinin or glycinin)could damage the structural integrity of the intestine,reduce immune defense,reshape the intestinal microbiome and,ultimately,impair growth in fish.

关键词： β-Conglycinin Glycinin Pearl gentian groupers Immune response Intestinal microbiota