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Cultivating Pleurotus ostreatus and Pleurotus eryngii mushroom strains onagro-industrial residues in solid-state fermentation. Part II: Effect onproductivity and quality of carposomes

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Carbon Resources Conversion 2022年第1期5卷 52-60页

作者： Eirini-Maria Melanouri Marianna Dedousi Panagiota Diamantopoulou Laboratory of Edible Fungi Institute of Technology of Agricultural ProductsHellenic Agricultural Organization-Demeter1 S.Venizelou Street14123 LykovrysiAttiki Greece

The potential of selected Pleurotus ostreatus and P. eryngii wild-type and commercial strains to colonize andproduce carposomes after solid-state fermentation of five substrates constituted of agro-residues namely wheatstraw (WS), beech wood shavings (BWS), coffee residue (CR), barley and oats straw (BOS), rice bark (RB),supplemented with wheat bran was investigated. The effect of substrate composition on bioprocess feasibilitywas assessed for the different strains by quantitative (i.e., mushroom yield and Biological Efficiency-BE %) andcarposome qualitative parameters (i.e., weight, size, colour, firmness). P. ostreatus strains produced carposomesearlier than P. eryngii ones. Early fruiting formation was promoted in WS for P. ostreatus strains, whereas forP. eryngii the lowest values of earliness were observed on BOS substrate. As for crop productivity, P. ostreatusstrain AMRL 150 provided the highest BE (>70%) in all substrates except for RB, while P. ostreatus strain AMRL144 achieved the highest yield and BE in BOS and BWS (75.30 and 64.26%, respectively). P. eryngii stainsproduced less number but heavier carposomes. The highest values of BE% for these strains were recorded on WSand BOS substrates. The BE was further correlated to growth parameters of fungal vegetative phase. As formushroom colour, P. ostreatus were lighter than P. eryngii and BOS substrate promoted lightness in both *** P. ostreatus mushrooms were produced at CR. No differences in the whiteness and firmness were detectedamong P. eryngii mushrooms and at any substrate used. The data included in this paper showed that Pleurotusmushrooms can be cultivated on low- or zero-value agro-industrial residues of great financial and environmentalimportance towards the production of value-added food products.

关键词： Oyster mushrooms Biological efficiency Carposomes Pileus Stipe Colour Texture

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A rule-based semi-automatic method to map burned areas in Mediterranean using Landsat images–revisited and improved

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International Journal of Digital Earth 2021年第11期14卷 1602-1623页

作者： Nikos Koutsias Magdalini Plenioua Department of Environmental Engineering University of PatrasG.Seferi 2GR-30100 AgrinioGreece Forest Research Institute Hellenic Agricultural Organization DemeterGR-57006 VassilikaThessalonikiGreece

At local or regional scales,where Landsat has been extensively applied to monitor burned areas,semi-or fully automated methods are not very *** et al.([2013].“A Rule-Based Semi-Automatic Method to map Burned Areas:Exploring the USGS Historical Landsat Archives to Reconstruct Recent Fire History.”International Journal of Remote Sensing 34(20):7049–7068)developed a semi-automatic method to map burned areas consisted of a set of rules that are valid especially when the post-fire image has been captured shortly after the ***,the rule-based approach is not free of errors that eventually create limitations to adopt this method for reconstructing the fire history in a fully automated *** this work,we improved the method by revisited and improving the rules that have been developed in the first paper,especially for reducing the omission *** changes have been made to almost all rules;however,one of the rules has been revised and changed the most as compared to the others by also considering the short-wave infrared channel TM5(in Landsat-5 or Landsat-7).In this case,the algorithm could successfully capture the fire scar in dry vegetated areas as *** to the lack of reference data collected in the field,we used reference data collected by photo interpretation,and the accuracy assessment showed that these had a high agreement with the burned land mapping results.

关键词： Fire scar mapping burned land mapping automatic Landsat rule-based approach Mediterranean

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Utilization of coal fly ash and municipal sewage sludge in agriculture and for reconstruction of soils in disturbed lands： results of case studies from Greece and China

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International Journal of Coal Science & Technology 2018年第1期5卷 64-69页

作者： Christos D. Tsadilas Zhenqi Hu Yinli Bi Thomai Nikoli Institute of Industrial and Forage Crops Hellenic Agricultural Organization "DEMETER" 1 Theophrastos Street 413 35 Larissa Greece Institute of Land Reclamation and Ecological Restoration China University of Mining and Technology D11 Xueyuan Road Haidian District Beijing 100083 China

Coal fly ash （CFA） and municipal sewage sludge （MSS） management is a great concern worldwide. An alternative gaining high interest, is their use in agriculture and for reclamation of degraded lands. The purpose of this paper was to present very briefly the results of some case studies carried out in China and Greece related to land reclamation and agricultural use of CFA and MSS separately or combined. An experiment in Platanoulia area, central Greece showed clearly that CFA applied together with MSS at appropriate rates increased substantially wheat grain and biomass yield and improved soil quality （increased soil pH, organic matter content, total nitrogen, available P and boron）. In a long-term experiment carried out in Huaibei city, Anhui province, China with a reconstructed soil in a subsided land by using CFA, it was found that physicochemical characteristics （infiltration rate, bulk density, total nitrogen, available P and extractable K） tended to be improved over time. In another experiment in Rodia area central Greece, MSS application improved soil quality of limestone mining spoils from bauxite mining activities. Several other experiments with MSS in Greece showed a clear positive effect on cotton and maize yield and on soil quality.

关键词： Coal fly ash Municipal sewage sludge Soil quality Land reclamation

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Seed Germination Traits of Loquat (Eriobotrya japonica Lindl.) as Affected by Various Pre-Sowing Treatments (Cutting of Cotyledons, Removal of Perisperm, Moist Chilling and/or Exogenous Application of Gibberellin)

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Phyton-International Journal of Experimental Botany 2020年第3期89卷 645-656页

作者： Thiresia-Teresa Tzatzani Evangelia Basdeki Evangelia-Vasiliki Ladikou Marios-Ioannis NSotiras Georgios Panagiotakis Sryridon Lionakis Ioannis E.Papadakis Laboratory of Subtropical Plants and Tissue Culture Institute of Olive TreeSubtropical Plants and ViticultureHellenic Agricultural Organization-DEMETERChania73134Greece Laboratory of Genetics Department of BiotechnologyAgricultural University of Athens(Member of the European University for Smart Urban Coastal Sustainability-EU-CONEXUS)Athens11855Greece Laboratory of Pomology Department of Crop ScienceAgricultural University of Athens(Member of the European University for Smart Urban Coastal Sustainability-EU-CONEXUS)Athens11855Greece Laboratory of Pomology School of Agricultural SciencesHellenic Mediterranean UniversityHeraklion71410Greece

The purpose of this study was to investigate the effects of various presowing treatments on the germinability(final germination percentage)and germination rate of loquat seeds in order to increase seedling production in nurseries(applied research)as well as provide answers for important physiological issues related to loquat seeds and their seed coat(basic research).Three experiments were carried out with various pre-sowing *** treatments included full or partial removal of seed coat(perisperm),partial cutting of cotyledons as well as moist chilling at 5℃ for 13 days and/or soaking the seeds in water or 250 ppm gibberellic acid(GA_(3))solution for 24 *** to the results,cotyledons excision resulted in delayed germination,regardless of the presence or absence of the seed coat in comparison with the decoated seeds that demonstrated the highest germination rate amongst *** addition,even the partial excision of seed coats affected positively both the germinability and the germination rate,compared to the control-intact ***,control-intact seeds had a higher germination percentage when exposed to moist chilling independently of the application or not of gibberellin;while the combination of gibberellin application and moist chilling improved both the percentage and the rate of germination of decoated *** conclusion,the role of perisperm(seed coat)in the germination procedure of loquat seeds seems to be important,indicating the existence of seed coat-imposed dormancy on loquat ***,the existence of a mild endogenous embryo-dormancy on loquat is also discussed.

关键词： Cotyledon gibberellin seed coat seed testa Eriobotrya japonica plant propagation perisperm sexual propagation seedling dormancy

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Options for Managing Livestock Production Systems to Adapt to Climate Change

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Journal of Earth Science and Engineering 2014年第7期4卷 415-427页

作者： Evangelia N. Sossidou Eleni Tsiplakou George Zervas Veterinary Research Institute Hellenic Agricultural Organization-DEMETER Thessaloniki 57001 Greece Nutritional Physiology and Feeding Agricultural University of Athens Athens 11885 Greece

This paper discusses recent research findings together with management schemes in preventing and managing of thermal stress by handling external and internal factors in livestock production systems. Preventive measures against thermal stress are described as basic structural adjustments and the modifications that can be implemented readily, according to animal health and welfare requirements and refer to （1） environmental modification and thermal comfort in various housing systems; （2） action on animals, such as genetic selection for breeds resistant to infectious disease, parasites and climate extremes; （3） action on feed and （4） action on staff handling the animals. Moreover, measures to be taken both in situations of chronic thermal stress and heat or cold strokes are presented as they are applied to （1） limit stress, （2） monitor the temperature felt by animals, （3） adapt diet and drinking water supplies and （4） correct physiological imbalances. Examples are given for different farm species （cattle, sheep, goat, poultry and pigs） and different production systems （intensive, extensive and alternative）. The paper concludes with a practical guide for the effective handling of thermal stress at farm level, summarizing the results from recent research studies on the specific topic.

关键词： Livestock production management thermal comfort.

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Forests of Greece, Their Multiple Functions and Uses, Sustainable Management and Biodiversity Conservation in the Face of Climate Change

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Open Journal of Ecology 2021年第4期11卷 374-406页

作者： Konstantinos A. Spanos Asimina Skouteri Dionysios Gaitanis Panos V. Petrakis Ioannis Meliadis Panagiotis Michopoulos Alexandra Solomou Panagiotis Koulelis Evangelia V. Avramidou hellenic agricultural organization—Dimitra General Directorate of Agricultural Research Forest Research Institute Thessaloniki Greece hellenic agricultural organization—Dimitra General Directorate of Agricultural Research Institute of Mediterranean and Forest Ecosystems Hellenic Agricultural Organization Athens Greece

This is a review paper based on national and international literature, national inventories and empirical approach. A general overview of the forests, forest management and forest biodiversity of Greece are briefly summarized. The distribution of the main forest species and their ecosystems in the Geographic regions of Greece is presented in a tabular form. Their multiple functions and uses, their management type/regime, their risks and threats, protection and conservation measures are also synoptically described. Furthermore, adaptive forest management and silviculture as well as management of genetic diversity in order to face climate change are also analysed. Research priority areas on biodiversity and challenges for biodiversity research and related policy in Greece have been summarized. The new Rural Development Program (2014-2020), which is currently in the final phase of implementation, will take into consideration the forest itself and forest-related European and National legal obligations. Finally, major conclusions and research priority areas on future management and conservation of forests are highlighted.

关键词： Greece Forests Multiple Uses Management Biodiversity Conservation Forest Policy Climate Change Strategic Targets

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Quality and Sensory Properties of Reduced Fat Yoghurt Made with Addition of <i>β</i>-Glucans

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Food and Nutrition Sciences 2018年第4期9卷 390-402页

作者： Eleni C. Pappa Efthymia Kondyli William MacNaughtan Athanasia Kakouri Konstantinos Nesseris Cleanthes Israilides Dairy Research Department Institute of Technology of Agricultural Products Hellenic Agricultural Organization-DEMETER Katsikas Greece Division of Food Sciences School of Biosciences University of Nottingham Sutton Bonington Campus Loughborough UK DODONI S.A. 1 Tagmatarchi Kostaki Eleousa Greece Institute of Technology of agricultural Products Hellenic Agricultural Organisation-DEMETER 1 S.Venizelou Lycovrysi Greece

β-Glucans, isolated from Pleurotus citrinopileatus mushroom, were used in the manufacture of yoghurt from reduced fat cow milk with added proteins. β-Glucans were added to the milk in the form of a paste containing 1.21% (on a dry weight basis) of β-glucans, such that the final concentrations in the yoghurt were 0.3%, 0.4% and 0.5%. Control yoghurt was also manufactured from the same milk without the addition of β-glucans. The resultant yoghurts were examined for their physicochemical and microbial characteristics, gel formation and sensory properties and compared to the control at 1, 7, 14 and 21 days of storage. Results showed that the microbial population, pH, titratable acidity, ash and syneresis were not affected by the addition of different levels of glucans. The lowest fat, total solids and protein contents were recorded in the yoghurt containing 0.5% glucan. The yoghurt containing 0.3% β-glucan had the same viscosity as the control and the yoghurt containing 0.5% β-glucan had the lowest viscosity. Yoghurt with β-glucans received slightly higher scores for flavour, appearance and texture than the control. It was concluded that the incorporation of 0.3% or 0.4% β-glucans isolated from edible mushrooms, into the yoghurt does not affect the final product, which is similar or sensorially even better than the control.

关键词： β-glucans Functional Yoghurt Pleurotus citrinopileatus Mushroom

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Implementation of Forest Policy in Greece in Relation to Biodiversity and Climate Change

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Open Journal of Ecology 2018年第3期8卷 174-191页

作者： Konstantinos Spanos Dionysios Gaitanis Asimina Skouteri Panos Petrakis Ioannis Meliadis hellenic agricultural organization General Directorate of Agricultural Research Forest Research Institute Thessaloniki Greece hellenic agricultural organization General Directorate of Agricultural Research Institute of Mediterranean Forest Ecosys-tems Athens Greece

This is a review article based on literature (national and international) and empirical approach. A general overview on research priority areas on biodiversity and approaches and tools to provide information for forest policy implementation on biodiversity are briefly summarized. Challenges for biodiversity research and related policy in Europe and Greece are depicted. General information on forests, protected areas and forest management in Greece is also presented. Major actions and measures for conservation of forest biodiversity in Greece are described and analysed. The implementation of forest policy in Greece (including the adoption of International constitutional frame) in relation to biodiversity protection and climate change is also analysed. Priorities identified by the Strategic Plan of Rural Development 2007-2013 in Greece in order to adapt to climate change, are also presented. Furthermore, the National institutional framework (Legislation) and strategic targets for biodiversity conservation in Greece are synoptically presented. Finally, major conclusions and future challenges are highlighted.

关键词： Biodiversity Conservation Protected Areas Forest Policy Implementation Strategic Targets

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Carposome productivity of Pleurotus ostreatus and Pleurotus eryngii growing on agro-industrial residues enriched with nitrogen,calcium salts and oils

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Carbon Resources Conversion 2023年第2期6卷 150-165页

作者： Marianna Dedousi Eirini-Maria Melanouri Panagiota Diamantopoulou Laboratory of Edible Fungi Institute of Technology of Agricultural Products(ITAP)Hellenic Agricultural Organization-Dimitra1 S.Venizelou Str14123 LykovryssiGreece Laboratory of Food Microbiology and Biotechnology Department of Food Science and NutritionAgricultural University of Athens75 Iera Odos Str11855 AthensGreece

The suitability of the abundant agro-industrial residues wheat straw(WS;control),barley and oats straw(BOS)and rice husk(RH),supplemented with various sources of oils(sunflower,corn oil),nitrogen(peptone,yeast extract)and calcium salts(CaSO_(4)⋅2H_(2)O,CaCl2),as novel substrates in solid-state fermentation of selected Pleurotus ostreatus and *** mushrooms was *** possible effect of different additives on mycelial growth rate,biomass production and endoglucanase,laccase and lipase biosynthesis were ***,their impact on essential cultivation aspects(earliness,total mushroom yield,biological efficiency)and carposome quality parameters(weight,morphological characteristics)was *** fungi showed their highest growth rates on BOS substrates and the most positive implementation was CaSO_(4)⋅2H_(2)O 6%w/w(Kr=9.58 mm/day;***,Kr=9.42 mm/day;***),while different additives led to enhancement of biomass *** species demonstrated minimal levels of endoglucanase activity,with values ranging from 0.01 to 0.42 U/g of dry weight,regardless of the substrate and the stage of *** the contrary,the maximum values of laccase activity were observed at 50%of colonization on BOS and RH,while supplementation with nitrogen and calcium sources positively affected its *** and *** cultivated on BOS supplemented with peptone at 2 and 5%w/w,synthesized significant laccase amounts,i.e.,12,165.78 and 8,624.55 U/g d.w.,*** amounts of lipase were produced,especially in substrates supplemented with sunflower 2%w/w,in quantities up to 1.42 U/g d.w.,whereas the highest lipase activity was achieved by *** on WS supplemented with corn oil at 2%w/w,with a value of 4.25 U/g *** *** fermentation of Pleurotus species in polypropylene bags,WS and BOS supported faster colonization and shorter earliness period than RH substrates,whereas supplementation did no

关键词： Oyster mushrooms Residues Additives Biomass Endoglucanase Laccase Lipase Weight

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Sustainable energy management of solar greenhouses using open weather data on MACQU platform

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International Journal of agricultural and Biological Engineering 2018年第1期11卷 74-82页

作者： Li Li Jieyu Li Haihua Wang Ts.Georgieva K.P.Ferentinos K.G.Arvanitis N.A.Sigrimis Key Laboratory on Modern Precision Agriculture System Integration Research of Ministry of Education China Agricultural UniversityBeijing 100083China Key Laboratory of agricultural Information Acquisition Technology of Ministry of Agriculture China Agricultural UniversityBeijing 100083China Department of Automatics and Mechatronics University of RuseRuse 7017Bulgaria hellenic agricultural organization“demeter” Institute of Soil&Water ResourcesDept.of Agricultural Engineering61 Dimokratias Av.13561AthensGreece&Research Advisor at Geomations SAspinoff of Agricultural University of Athens Department of Natural Resources Management&agricultural Engineering Agricultural University of AthensAthens 11855Greece Director R&D Department Geosmart IKEspinout of Geomations of AUANafplio 21100Greece

Precision energy management is very important for sustainability development of solar greenhouses,since huge energy demand for agricultural production both in quantity and quality.A proactive energy management,according to the optimal energy utilization in a look-ahead period with weather prediction,is presented and tested in this research.A multi-input-multi-output linear model of the energy balance of solar greenhouses based on on-line identification system can simulate greenhouse behavior and allow for predictive *** good time allocation of available solar energy can be achieved by intelligent use of controls,such as store/retrieve fans and ventilation windows,*** energy to warm up the air or to be stored in the storage elements(wall,soil,etc.)or to be exhausted to *** proactive energy management can select an optimal trajectory of air temperature for the forecasted weather period to minimize plants’thermal‘cost’defined by an‘expert’in terms of set-points for the specific *** selection of temperature trajectory is formulated as a generalized traveling salesman problem(GTSP)with precedence constraints and is solved by a genetic algorithm(GA)in this *** simulation study showed good potential for energy saving and timely allocation to prevent excessive crop *** active control elements in addition to predefining and applying,within energy constraints,optimal climate in the greenhouse,it also reduces the energy deficit,*** working hours of the‘heater’in the sustained freezing weather,as well as the ventilation hours,that is,more energy harvest in the warm *** intelligent solar greenhouse management system is being migrated to the web for serving a‘customer base’in the Internet Plus *** capacity,of the concrete ground CAUA system(CAUA is an abbreviations from both China agricultural University and agricultural University of Athens),to implement web‘updates’of criteria,open weather data and models,on which control

关键词： solar greenhouse precision energy management energy-saving open weather data traveling salesman optimization

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