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Multivariate statistical and bioinformatic analyses for the seasonal variations of actinobacterial community structures in a drinking water reservoir

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Journal of Environmental Sciences 2024年第3期137卷 1-17页

作者： Haihan Zhang Sixuan Pan BenMa Tinglin Huang Dmitry B.Kosolapov Manli Ma Xiang Liu Huan Liu Xiaoyan Liu Shaanxi key laboratory of Environmental Engineering Key Laboratory of Northwest Water ResourceEnvironment and EcologyMOEXi’an University of Architecture and TechnologyXi’an 710055China School of Environmental and Municipal Engineering Xi’an University of Architecture and TechnologyXi’an 710055China Papanin Institute for Biology of Inland Waters of Russian Academy of Sciences(IBIW RAS) 109BorokNekouzYaroslavl152742Russia

Actinobacterial community is a conspicuous part of aquatic ecosystems and displays an important role in the case of biogeochemical cycle,but little is known about the seasonal variation of actinobacterial community in reservoir ecological *** this study,the high-throughput techniques were used to investigate the structure of the aquatic actinobacterial community and its inducing water quality parameters in different *** results showed that the highest diversity and abundance of actinobacterial community occurred in winter,with Sporichthya(45.42%)being the most abundant genus and Rhodococcus sp.(29.32%)being the most abundant *** analysis and correlation analysis suggested that in autumn the dynamics of actinobacterial community were infuenced by more factors and Nocardioides ***2R5S2 was the potential keystone species which was negatively correlated with temperature(R=-0.72,Pturbidity are potential conspicuous factors infuencing seasonal actinobacterial community *** partial least squares path modeling further elucidated that the combined effects of DO and temperature not only in the diversity of actinobacterial community but also in other water qualities,while the physiochemical parameters(path coefficient=1.571,P<0.05)was strong environmental factors in natural mixture *** results strengthen our understanding of the dynamics and structures of actinobacterial community in the drinking water reservoirs and provide scientific guidance for further water quality management and protection in water sources.

关键词： Source water reservoir High-throughput sequencing Correlation analysis Network analysis Partial least squares path modeling

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Toward a rapid and convenient nanoplastic quantification method in laboratory-scale study based on fluorescence intensity

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Frontiers of Environmental Science & Engineering 2024年第5期18卷 117-126页

作者： Ruiqi Yan Sen Lin Qian Ding Lei Zhang Xia Yu Wentao Zhao Qian Sui State Environmental Protection key laboratory of Environmental Risk Assessment and Control on Chemical Process School of Resources and Environmental EngineeringEast China University of Science and TechnologyShanghai200237China State key laboratory of Pollution Control and Resource Reuse College of Environmental Science and EngineeringTongji UniversityShanghai200092China Shanghai Institute of Pollution Control and Ecological Security Shanghai200092China

The thorough investigation of nanoplastics(NPs)in aqueous environments requires efficient and expeditious quantitative analytical methods that are sensitive to environmentally relevant NP concentrations and convenient to *** analysis-based quantitative methods have been acknowledged as effective and rapid approaches for quantifying NP concentrations in laboratory-scale ***,we compared three commonly used optical response indicators,namely fluorescence intensity(FI),ultraviolet absorbance,and turbidity,to assess their performance in quantifying ***,orthogonal experiments were conducted to evaluate the influence of various water quality parameters on the preferred indicator-based quantification *** results revealed that FI exhibits the highest correlation coefficient(>0.99)with NP ***,the limit of quantification(LOQ)for various types of NPs is exceptionally low,ranging from 0.0089 to 0.0584 mg/L in ultrapure water,well below environmentally relevant *** variations in water quality parameters such as pH,salinity,suspended solids(SS),and humic acid,a robust relationship between detectable FI and NP concentration was ***,an increased matrix,especially SS in water samples,results in an enhanced LOQ for ***,the quantitative method remains applicable in real water bodies,especially in drinking water,with NP LOQ as low as 0.0157–0.0711 mg/*** exceeds the previously reported detectable concentration for 100 nm NPs at 40µg/mL using surface-enhanced Raman *** study confirms the potential of FI as a reliable indicator for the rapid quantification of NPs in aqueous environments,offering substantial advantages in terms of both convenience and cost-effectiveness.

关键词： Nanoplastic quantification Fluorescence intensity Drinking water Limit of quantification(LOQ) Matrix effects

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Distribution of Traditional Chinese Medicine syndromes in diabetic kidney disease chronic kidney disease 1-5: a correlation study

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Journal of Traditional Chinese Medicine 2024年第3期44卷 572-580页

作者： LI Shuang DUAN Shuwei DONG Zheyi QU Yilun LUO Yayong KE Jianghua WANG Conghui PENG Yangzhi ZHOU Xuefeng CHEN Xiangmei Department of Nephrology First Medical Center of Chinese PLA General HospitalNephrology Institute of the Chinese People's Liberation ArmyNational Key Laboratory of Kidney DiseasesNational Clinical Research Center for Kidney DiseasesBeijing Key Laboratory of Kidney Disease Research School of Clinical Medicine Guangdong Pharmaceutical University Basic Medical College Chengdu University of Traditional Chinese Medicine

OBJECTIVE: To analyze the distribution of Traditional Chinese medicine(TCM) syndromes in patients with diabetic kidney disease(DKD) and its related ***: We enrolled 435 patients with DKD, who were not undergoing dialysis, admitted to the Department of Nephrology, First Medical Center, Chinese PLA General Hospital from April 2020 to August *** of their TCM syndromes and related factors was carried ***: The 435 patients included 109, 117, 86, and 123 chronic kidney disease(CKD) 1-2, CKD3, CKD4, and CKD5 cases, respectively. With the progression of CKD1-5, the proportion of Yin deficiency and dry heat syndrome,and that of Qi and Yin deficiency syndrome showed a downward trend, whereas the proportion of spleen-kidney Yang deficiency, blood deficiency, blood stasis, water stagnation, and phlegm turbidity syndromes showed an upward trend; the differences were statistically significant(P < 0.05). Multivariate logistic regression analysis showed that Yin deficiency and dry heat syndrome was positively correlated with hemoglobin [odds ratio(OR) =1.022, P = 0.005], albumin(OR = 1.058, P = 0.006), and estimated glomerular filtration rate(eGFR)(OR = 1.020,P < 0.001) but negatively correlated with male sex(OR =0.277, P = 0.004). Qi and Yin deficiency syndrome was positively correlated with albumin(OR = 1.056, P < 0.001)and eGFR(OR = 1.008, P = 0.022) but negatively correlated with age(OR = 0.977, P = 0.023). Liver-kidney Yin deficiency syndrome was positively correlated with age(OR = 1.028, P = 0.021) and glycosylated hemoglobin(OR = 1.223, P = 0.007) but negatively correlated with total cholesterol(OR = 0.792, P = 0.006).Spleen-kidney Yang deficiency syndrome was negatively correlated with hemoglobin(OR = 0.977, P < 0.001),albumin(OR = 0.891, P < 0.001), and eGFR(OR = 0.978,P < 0.001) but positively correlated with high density lipoprotein(OR = 3.376, P = 0.001). CONCLUSION: With CKD1-5 progression, TCM syndromes changed from Yin deficiency and dry

关键词： albumins correlation of data diabetic nephropathies glomerular filtration rate hemoglobins syndrome and complex

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Bioinspired Polarized Optical Flow Enables Turbid Underwater Target Motion Estimation

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Journal of Ocean University of China 2024年第4期23卷 915-923页

作者： CHENG Haoyuan ZHAO Shujie ZHU Jinchi YU Hao CHU Jinkui College of Engineering Ocean University of ChinaQingdao 266100China key laboratory for Micro/Nano Technology and System of Liaoning Province Dalian University of TechnologyDalian 116024China

Underwater target motion estimation is a challenge for ocean military and scientific *** this work,we propose a method based on the combination of polarization imaging and optical flow for turbid underwater target *** imaging can reduce the influence of backscattered light and obtain high-quality images *** optical flow shows the motion and structural information of the *** use polarized optical flow to obtain the optical flow field and estimate the target *** experimental results of different targets under varying water turbidity levels illustrate that our method is realizable and *** precision is verified by comparing the results with the precise displacement data and calculating two error *** proposed method based on polarized optical flow can obtain accurate displacement information and a good recognition *** target segmentation based on the Otsu method further proves the superiority of the polarized optical flow under turbid *** study is valuable for target detection and motion estimation in scattering environments.

关键词： turbid underwater motion estimation polarization imaging optical flow

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Predicting impact forces on pipelines from deep-sea fluidized slides:A comprehensive review of key factors

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International Journal of Mining Science and Technology 2024年第2期34卷 211-225页

作者： Xingsen Guo Ning Fan Defeng Zheng Cuiwei Fu Hao Wu Yanjun Zhang Xiaolong Song Tingkai Nian State key laboratory of Coastal and Offshore Engineering Dalian University of TechnologyDalian 116024China Department of Civil Environmentaland Geomatic EngineeringUniversity College LondonLondon WC1E 6BTUnited Kingdom Shandong Provincial key laboratory of Marine Environment and Geological Engineering Ocean University of ChinaQingdao 266100China State key laboratory of Geohazard Prevention and Geoenvironment Protection Chengdu University of TechnologyChengdu 610059China College of Civil Engineering and Architecture Wenzhou UniversityWenzhou 325035China key laboratory of Engineering and Technology for Soft Soil Foundation and Tideland Reclamation of Zhejiang Province Wenzhou 325035China Zhejiang International Science and Technology Cooperation Base of Ultra-soft Soil Engineering and Smart Monitoring Wenzhou 325035China School of Geography Liaoning Normal UniversityDalian 116029China China Railway First Survey and Design Institute Group Co. Ltd.Xi’an 710043China Department of Geotechnical Engineering Nanjing Hydraulic Research InstituteNanjing 210024China Wuhan Center China Geological Survey(Central South China Innovation Center for Geosciences)Wuhan 430205China State key laboratory of Mining Response and Disaster Prevention and Control in Deep Coal Mines Anhui University of Science and TechnologyHuainan 232001China

Deep-sea pipelines play a pivotal role in seabed mineral resource development,global energy and resource supply provision,network communication,and environmental ***,the placement of these pipelines on the seabed surface exposes them to potential risks arising from the complex deep-sea hydrodynamic and geological environment,particularly submarine *** incidents have highlighted the substantial damage to pipelines due to ***,deep-sea fluidized slides(in a debris/mud flow or turbidity current physical state),characterized by high speed,pose a significant *** assessing the impact forces exerted on pipelines by fluidized submarine slides is crucial for ensuring pipeline *** study aimed to provide a comprehensive overview of recent advancements in understanding pipeline impact forces caused by fluidized deep-sea slides,thereby identifying key factors and corresponding mechanisms that influence pipeline impact *** factors include the velocity,density,and shear behavior of deep-sea fluidized slides,as well as the geometry,stiffness,self-weight,and mechanical model of ***,the interface contact conditions and spatial relations were examined within the context of deep-sea slides and their interactions with *** upon a thorough review of these achievements,future directions were proposed for assessing and characterizing the key factors affecting slide impact loading on pipelines.A comprehensive understanding of these results is essential for the sustainable development of deep-sea pipeline projects associated with seabed resource development and the implementation of disaster prevention measures.

关键词： Deep-sea fluidized slides Pipes Impact forces Shear behavior of slides Interface contact conditions Spatial relation

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The important role of turbidity Maximum Zone in sedimentary dynamic of estuarine mangrove swamp

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Frontiers of Earth Science 2024年第1期18卷 219-226页

作者： Tao LIU Ying LIU Baoqing HU key laboratory of Environment Change and Resources Use in Beibu Gulf(Ministry of Education) Nanning Normal UniversityNanning 530001China School of Civil Engineering and Architecture Guangxi UniversityNanning 530004China

Sedimentation is a key process affecting wetland sustainability and carbon burial *** context of sea level rise,climate change and human activities,further understanding about the sedimentary dynamic in wetland is critical in predicting the landscape evolution or the change in carbon burial *** this study,based on the field hydrological observation in a mangrove system in the Nanliu River estuary,we found the net flux of suspended sediment to mangrove is 39−72 kg/m in tidal cycles with turbidity Maximum Zone(TMZ)forming in surface layer and only is 9−18 kg/m in tidal cycles without *** higher net flux of suspended sediment to mangrove in tidal cycles with TMZ forming in surface layer is attributed to high SSC in rising tide and intense flocculation in *** significant discrepancy in sedimentation rate in the mangrove patches also can be explained by the probability of TMZ forming in the surface layer of *** future,rapid sea level rising may lead to the change of TMZ pattern in estuary,which will result in non-negligible variation in sedimentation rate in *** to the present data of sedimentation rate in wetlands,the fragility of wetlands in river estuary may be miscalculated.

关键词： mangrove wetland sedimentation turbidity Maximum Zone Nanliu River

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Experimental investigation of the inhibition of deep-sea mining sediment plumes by polyaluminum chloride

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International Journal of Mining Science and Technology 2024年第1期34卷 91-104页

作者： Fengpeng Zhang Xuguang Chen Jiakang Wei Yangyang Zhang Weikun Xu Hao Li College of Engineering Ocean University of ChinaQingdao 266100China Shandong Province key laboratory of Ocean Engineering Ocean University of ChinaQingdao 266100China China Merchants Marine and Offshore Research Institute Co. Ltd.Shenzhen 518000China National Deep Sea Center Qingdao 266237China

Deep-sea sediment disturbance may occur when collecting polymetallic nodules,resulting in the creation of plumes that could have a negative impact on the ecological *** study aims to investigate the potential solution of using polyaluminum chloride(PAC)in the water *** effects of PAC are examined through a self-designed simulation system for deep-sea polymetallic nodule collection and sediment samples from a potential deep-sea mining *** experimental results showed that the optimal PAC dose was found to be 0.75 g/*** with the test conditions without the addition of PAC,the presence of PAC leads to a reduction in volume,lower characteristic turbidity,smaller diffusion velocity,and shorter settling time of the *** indicates that PAC inhibits the entire development process of the *** addition of PAC leads to the flocculation of mm-sized particles,resulting in the formation of cm-sized *** flocculation of particles decreases the rate of erosion on the seabed by around 30%.This reduction in erosion helps to decrease the formation of ***,when the size of suspended particles increases,it reduces the scale at which they ***,the settling velocity of flocs(around 10^(-2) m/s)is much higher that of compared to sediment particles(around 10^(-5) m/s),which effectively reduces the amount of time the plume remains in suspension.

关键词： Deep-sea mining Deep-sea polymetallic nodules Sediment plume Polyaluminum chloride Jet impact Particle flocculation

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Water quality and habitat drive phytoplankton taxonomic and functional group patterns in the Yangtze River

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Ecological Processes 2024年第1期13卷 144-158页

作者： Wenqi Gao Fangyuan Xiong Ying Lu Wei Xin Haihua Wang Gangpeng Feng Chiping Kong Lei Fang Xiaoping Gao Yushun Chen State key laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences University of Chinese Academy of Sciences Jiangxi Institute for Fisheries Sciences Poyang Lake Fisheries Research Centre of Jiangxi Province East China Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Jiujiang Institute of Agricultural Sciences

Background Although phytoplankton are important primary producers in food webs, they are relatively less studied in large rivers compared to other types of systems. To fill this research gap, we studied phytoplankton taxonomic and functional composition and their relationships with water quality, habitat, climate, and land use across 30 river sections in the middle and lower reaches of the Yangtze River during 2017–*** Major observed phytoplankton groups were cyanobacteria, bacillariophyta, and chlorophyta. Phytoplankton total abundance, total biomass, and species richness significantly decreased in the dry season compared to the wet season, with the species and functional composition differing significantly between seasons. Phytoplankton species differences between seasons were mainly contributed by Oscillatoria sp., Pseudanabaena sp., and Melosira granulata. The dfferences in phytoplankton functional groups between seasons were mainly contributed by P（including Closterium sp., Melosira sp.）, Lo（including Merismopedia sp., Peridinium sp., Ceratium sp., and Gymnodinium sp.）, and J（including Pediastrum sp., Tetraedron sp., Crucigenia sp., Scenedesmus sp., and Coelastrum sp.）. The variance partitioning showed that water quality（NO3-N, total suspended solids, turbidity） and habitat（water flow, river bank and river channel conditions） were critical factors in shaping phytoplankton patterns, followed by climate and land *** Results indicated that there was significant seasonal variation of phytoplankton in the Yangtze River, with water quality and habitat primarily driving phytoplankton patterns. Our study contributes to the understanding of natural and anthropogenic factors that drive seasonal successional processes of phytoplankton in the Yangtze River. These findings have important implications for environmental management as well as towards the ecological restoration of large rivers.

关键词： Yangtze River Phytoplankton Functional group Water quality Physical habitat

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Portable fluorescence instrument for detecting membrane integrity in membrane bioreactor (MBR)

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Frontiers of Environmental Science & Engineering 2024年第2期18卷 121-131页

作者： Yang Yu Changchun Xin Yuxiang Liu Fei Gao Lei Zhang Hui Jia Jie Wang State key laboratory of Separation Membranes and Membrane Processes Tiangong UniversityTianjin 300387China School of Environmental Science and Engineering Tiangong UniversityTianjin 300387China School of Environmental Science and Engineering Tianjin UniversityTianjin 300072China Shenyang Academy of Environmental Sciences Shenyang 110167China Cangzhou Institute of Tiangong University Tiangong UniversityCangzhou 061000China

This study proposed the design, fabrication, and assembly of membrane integrity detection instruments in membrane bioreactors (MBR) based on fluorescence spectroscopy. Based on the PARAFAC model, we found that the peak at 280/335 nm strengthened after membrane breakage. The peak at 340/430 nm reflected the sludge concentration in the MBR and reduced the influence of internal filtration effects on detection. Therefore, we determined that the dual-LED light source excitation detection system can detect tryptophan-like substances at 280 nm (T-peak) and humic acid at 340 nm (C-peak). T-peak was identified as the core index indicating membrane integrity. Moreover, the C-peak is the reference indicator factor for a sensitive response to changes in the sludge concentration. The portable fluorescence instrument exhibited high sensitivity and good feedback accuracy compared to particle counting and turbidity detection, where the log reduction value was greater than 3.5. This overcomes the disadvantage of false alarms in particle counters and is not affected by the position of the pump system. This portable instrument provides a flexible and highly sensitive method for the assessment of industrial membrane integrity.

关键词： Membrane integrity Fluorescence spectrum Portable instrument Membrane bioreactor

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Synthesis of chitosan-based grafting magnetic flocculants for flocculation of kaolin suspensions

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Journal of Environmental Sciences 2024年第5期139卷 193-205页

作者： Chuang Liu Xiaoyu Wang Sicong Du Wenyan Liang Beijing key Lab for Source Control Technology of Water Pollution/Engineering Research Center for Water Pollution Source Control&Eco-remediation/College of Environmental Science and Engineering Beijing Forestry UniversityBeijing 100083China

A series of novel chitosan-based magnetic flocculants FS@CTS-P(AM-DMC)was prepared by molecular structure *** characterization results showed that FS@CTS-P(AM-DMC)had a uniform size of about 21.46 nm,featuring a typical core-shell structure,and the average coating layer thickness of CTS-P(AM-DMC)was about 5.03 ***@CTS-P(AM-DMC)exhibited excellent flocculation performance for kaolin suspension,achieved 92.54% turbidity removal efficiency under dosage of 150 mg/L,pH 7.0,even at high turbidity(2000 NTU)with a removal efficiency of 96.96%.The flocculation mechanism was revealed to be dominated by charge neutralization under acidic and neutral conditions,while adsorption and bridging effects play an important role in alkaline *** properties of magnetic aggregates during flocculation,breakage,and regeneration were studied at different pH levels and *** the process of magnetophoretic,magnetic particles collide and adsorb with kaolin particles continuously due to magnetic and electrostatic attraction,transform into magnetic chain clusters,and then further form three-dimensional network magnetic aggregates that can capture free kaolin particles and other chain *** image velocimetry confirmed the formation of eddy current of magnetic flocs and experienced three stages:acceleration,stabilization,and deceleration.

关键词： Magnetic flocculation Chitosan Fe_(3)O_(4) Graft copolymerization PIV

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