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A Mutation of OSOTP 51 Leads to Impairment of Photosystem I Complex Assembly and Serious Photo-damage in Rice

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Journal of Integrative plant Biology 2012年第2期54卷 87-98页

作者： Jian-Wei Ye Zi-Ying Gong Chun-Guang Chen Hua-Ling Mi Gen-Yun Chen National key laboratory of plant molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological SciencesChinese Academy of Sciences Shanghai 200032 China

Gene expression in chloroplasts is regulated by many nuclear-encoded proteins. In this study, we isolated a rice （Oryza sativa subsp, japonica） mutant osotp51 with significant reduction in photosystem I （PSI）. The osotp51 is extremely sensitive to light and accumulates a higher level of reactive oxygen species. Its leaves are almost albino when grown at 40 ~mol photons/m2 per s. However, grown at 4 umol photons/m2 per s, osotp51 has a similar phenotype to the wild-type. 77K chlorophyll fluorescence analysis showed a blue shift in the highest peak emission from PSI in osotp51. In addition, the level of PSI and PSII dimer is dramatically reduced in osotp51. OSOTP 51 encodes a pentatricopeptide repeats protein, homologous to organelle transcript processing 51 in Arabidopsis. Loss-of-function OSOTP51 affects intron splicing of a number of plastid genes, particularly the ycf3 coding a protein involved in the assembly of PSI complex. OSOTP51 is functionally conserved in higher plants. The mutation of osotp51 indirectly leads to a widespread change in the structure and functions of PSI, results in severe photoinhibition, and finally dies, even when grown under very low light intensity.

关键词： Chloroplast photoprotection photosynthesis splicing ycf3

Interaction of rice bZIP protein REB with the 5'-upstream region of both rice sbe1 gene and waxy gene

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Chinese Science Bulletin 2002年第4期47卷 310-314页

作者： CAI Yi, XIE Donglu, WANG Zongyang & HONG MengminState key laboratory of plant molecular Genetics, Shanghai Institute of plant physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China State key laboratory of plant molecular Genetics Shanghai Institute of Plant Physiology and Ecology Chinese Academy of Sciences Shanghai China

Rice starch branching enzyme 1 (SBE1) and granule-bound starch synthase (GBSS) catalyzed the bio-synthesis of amylopectin and amylose in rice developing en-dosperm respectively, and the genes encoding these two en-zymes, sbe1 and waxy, are mainly expressed in the en-dosperm. Within the 5 ’-upstream region of rice sbel gene, we identified a 53 bp fragment C53 which could interact with the nuclear proteins extracted from rice endosperm. We also found that fragment Ha-2 in the 5 ’-upstream region of rice waxy gene could compete with C53 for its interaction. Fur-ther experiments demonstrated that rice bZIP protein REB could interact with two ACGT elements (G-box and Hex) in the fragment C53 as well as three ACGT elements (WG1, WG2, and WG3) in the fragment Ha-2. The WG1 element could compete well with Hex element for its interaction with REB. These results suggested that the expression of sbel and waxy gene in rice developing endosperm may be coordinately regulated by REB-like bZIP transcriptional factors.

关键词： rice waxy gene sbel gene bZIP protein.

DELAYED FLOWERING, an Arabidopsis Gene That Acts in the Autonomous Flowering Promotion Pathway and Is Required for Normal Development

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Journal of Integrative plant Biology 2006年第1期48卷 27-34页

作者： Ming-Jie Chen Zheng Yuan Hai Huang key laboratory of molecular Genetics Shanghai Institute of Plant Physiology and Ecology Graduate School of the Chinese Academy of Sciences Shanghai 200032 China

The control of flowering time in higher plants is one of the most important physiological processes and is critical for their reproductive success. To investigate the mechanisms controlling flowering time, we screened for Arabidopsis mutants with late-flowering phenotypes. One mutant, designated delayed flowering （dfr） in the Landsberg erecta （Ler） ecotype, was identified with delayed flowering time. Genetic analysis revealed that dfr is a single gene recessive nuclear mutant and the mutation was mapped to a locus tightly linked to UFO on chromosome 1. To our knowledge, no gene regulating flowering time has been reported yet in this region. The dfrmutant plant showed a delayed flowering time under the different growth conditions examined, including long- and short-day photoperiods and gibberellic acid GA3 treatments, suggesting that DFR is a gene involved in the autonomous flowering promotion pathway. The Arabidopsis gene FLOWERING LOCUS C （FLC） plays a central role in repressing flowering and its transcripts are undetectable in wild-type Ler. However, FLCexpression was upregulated in the dfrmutant, suggesting that DFR is a negative regulator of FLC. In addition, the dfr mutant plant displayed altered valve shapes of the silique and the number of trichomes and branches of each trichome were both reduced, indicating that the DRFgene is also required for normal plant development. Moreover, dfr leafy-5 （Ify-5） double mutant plants showed a much later flowering time than either dfr or Ify-5 single mutants, indicating that DFR and LFY act synergistically to promote flowering in Arabidopsis.

关键词： Arabidopsis autonomous pathway dfr, flowering time trichome.

Statistics as Part of Scientific Reasoning in plant Sciences:Overlooked Issues and Recommended Solutions

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molecular plant 2019年第1期12卷 7-9页

作者： Ying Zhang Chi-Kuang Wen National key laboratory of plant molecular Genetics CAS Center for Excellence in Molecular Plant Sciencesinstitute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China

New research tools for modern life sciences are emerging every few years and being implemented to reveal the underlying mechanisms of scientific questions of ***,statistical practice in modern life sciences has barely moved forward and probably has *** many cases,the decisions from biological experiments are predominantly made by using significant P values involving inappropriate statistical analyses and standards,and positive,favored results are preferentially reported (Fanelli,2012;Head et al.,2015;Demidenko,2016;Yong, 2017).

关键词： life research questions

Immunophilin-like FKBP42/TWISTED DWARF1 Interacts with the Receptor Kinase BRI1 to Regulate Brassinosteroid Signaling in Arabidopsis

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molecular plant 2016年第4期9卷 593-600页

作者： Juthamas Chaiwanon Veder J. Garcia Heather Cartwright Ying Sun Zhi-Yong Wang Department of plant Biology Carnegie Institution for Science Stanford CA 94305 USA Department of Botany Center of Excellence in Environment and Plant Physiology Faculty of Science Chulalongkorn University Bangkok 10330 Thailand present address: Institute of molecular Cell Biology Hebei Key Laboratory of Molecular and Cellular Biology Hebei Normal University Shijiazhuang 050024 China

Mutation of the immunophilin-like FK506-binding protein TWlSTED DWARF1 （FKBP42/TWD1） causes dwarf and twisted-organ phenotypes in Arabidopsis. However, the function of FKBP42 is not fully understood at the molecular level. Using genetic, physiological, and immunological experiments, we show here that FKBP42/TWD1 is necessary for brassinosteroid （BR） signal transduction. The twdl mutant showed reduced BR sensitivity in growth responses and activation of the BZR1 transcription factor. However, twdl showed normal responses to an inhibitor of BIN2/GSK3, suggesting that twdl has a defect upstream of BIN2 in the BR signaling pathway. In vitro and in vivo assays showed that TWD1 interacts physically with the kinase domains of the BR receptor kinases BRI1 and BAK1. TWD1 is not required for normal localization of BRI1-GFP to the plasma membrane or for activation of the flagellin receptor kinase FLS2. Our results suggest that FKBP42/TWD1 plays a specific role in the activation of BRI1 receptor kinase.

关键词： brassinosteroid signaling BRI1 FKBP42

Postulation of Leaf Rust Resistance Genes in Seven Chinese Spring Wheat Cultivars

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Journal of Integrative Agriculture 2013年第9期12卷 1580-1588页

作者： SHI Li-hong ZHANG Na HU Ya-ya WEI Xue-jun YANG Wen-xiang LIU Da-qun Department of plant pathology College of Plant Protection/Biological Control Center for Plant Diseases and Plant Pests of HebeiAgricultural University of Hebei

To detect the leaf rust resistance genes in the 7 Chinese spring wheat clultivars Shenmian 99025, Shenmia 99042, Shenmian 85, Shenmian 91, Shenmian 96, Shenmian 1167 and Shenmian 962, Thatcher, Thatcher backgrounded near-isogenic lines and 15 pathotypes of P. triticina were used for gene postulate at the seedling stage, and 9 of the 15 pathotypes were used in the field tests. molecular markers closely linked to, or co-segregated with resistance genes Lrl, Lr9, LrlO, Lrl9, Lr20, Lr21, Lr24, Lr26, Lr28, Lr29, Lr32, Lr34, Lr35, Lr37, Lr38, and Lr47 were screened to assist detection of the resistance genes. As results, 4 known resistance genes, including Lrl, Lr9, Lr26, and Lr34, and other unknown resistance genes were postulated singly or in combination in the tested cultivars. Shenmian 85, Shenmian 91, Shenmian 96, Shenmian 962, Shenmian 1167, and Shenmian 99042 are potentially useful for wheat production and breeding programs. The result suggested that combining gene postulation, molecular markers and pedigrees is effective and more accuracy method to know the resistance genes in cultivars.

关键词： gene postulation molecular marker Triticum aestivum Puccinia triticina resistance gene

Transcriptome-wide Analysis Of Vernalization Reveals Conserved and Species-specific Mechanisms in Brachypodium

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Journal of Integrative plant Biology 2013年第8期55卷 696-709页

作者： Qing Huan Zhiwei Mao Jingyu Zhang Yunyuan Xu Kang Chong key laboratory of plant molecular physiology Institute of Botanythe Chinese Academy of Sciences University of Chinese Academy of Sciences the Chinese Academy of Sciences

Several temperate cereals need vernalization to promote flowering. Little, however, is known about the vernalization-memory-related genes, and almost no comparative analysis has been performed. Here, RNA- Seq was used for transcriptome analysis in non-vernalized, vernalized and post-vernalized Brachypodium distachyon （L.） Beauv. seedlings. In total, the expression of 1,665 genes showed significant changes （fold change 〉4） in response to vernalization. Among them, 674 putative vernalization-memory-related genes with a constant response to vernalization were significantly enriched in transcriptional regulation and monooxygenase-mediated biological processes. Comparative analysis of vernalization-memory-related genes with barley demonstrated that the oxidative-stress response was the most conserved pathway between these two plant species. Moreover, Brachypodium preferred to regulate transcription and protein phosphorylation processes, while vernalization-memory-related genes, whose products are cytoplasmic membrane-bound-vesicle-located proteins, were preferred to be regulated in barley. Correlation analysis of the vernalization-related genes with barley revealed that the vernalization mechanism was conserved between these two plant species. In summary, vernalization, including its memory mechanism, is conserved between Brachypodium and barley, although several species-specific features also exist. The data reported here will provide primary resources for subsequent functional research in vernalization.

关键词： Brachypodium comparative analysis RNA-Seq vernalization.

Rise of the ethylene biosynthesis machinery from the Cβ-S lyase

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molecular plant 2022年第5期15卷 784-787页

作者： Mengchen Tong Chi-Kuang Wen National key laboratory of plant molecular Genetics CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China

1-aminocyclopropane-1-carboxylic acid(ACC),produced from S-adenosyl-methionine(SAM)by ACC synthase(ACS),is oxidized by ACC oxidase(ACO)to produce the gaseous plant hormone ethylene in seed plants(Kende,1993).

关键词： oxidized machinery gaseous

STRIPE2 Encodes a Putative dCMP Deaminase that Plays an Important Role in Chloroplast Development in Rice

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Journal of Genetics and Genomics 2014年第10期41卷 539-548页

作者： Jing Xu Yiwen Deng Qun Li Xudong Zhu Zuhua He National key laboratory of plant molecular Genetics and National Center of plant Gene Research Institute of Plant Physiology and EcologyChinese Academy of Sciences China National Rice Research Institute

Mutants with abnormal leaf coloration are good genetic materials for understanding the mechanism of chloroplast development and chlorophyll biosynthesis. In this study, a rice mutant st2 （stripe2） with stripe leaves was identified from the ＂y-ray irradiated mutant pool. The st2 mutant exhibited decreased accumulation of chlorophyll and aberrant chloroplasts. Genetic analysis indicated that the st2 mutant was controlled by a single recessive locus. The ST2 gene was finely confined to a 27-kb region on chromosome 1 by the map-based cloning strategy and a 5-bp deletion in Os01g0765000 was identified by sequence analysis. The deletion happened in the joint of exon 3 and intron 3 and led to new spliced products of mRNA. Genetic comptementation confirmed that Os01g0765000 is the ST2 gene. We found that the ST2 gene was expressed ubiquitously. Subcellular localization assay showed that the ST2 protein was located in mitochondria. ST2 belongs to the cytidine deaminase-like family and possibly functions as the dCMP deaminase, which catalyzes the formation of dUMP from dCMP by deamination. Additionally, exogenous application of dUMP could partially rescue the st2 phenotype. Therefore, our study identified a putative dCMP deaminase as a novel regulator in chloroplast development for the first time.

关键词： stripe2 Chloroplast development dCMP deaminase Oryza sativa