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Risk assessment and ranking of pesticide residues in Chinese pears

Journal of Integrative Agriculture 2015年第11期14卷 2328-2339页

作者： LI Zhi-xia NIE Ji-yun YAN Zhen XU Guo-feng LI Hai-fei KUANG Li-xue PAN Li-gang XIE Han-zhong WANG Cheng LIU Chuan-de ZHAO Xu-bo GUO Yong-ze Institute of Pomology Chinese Academy of Agricultural Sciences/Laboratory of Quality & Safety Risk Assessment for Fruit (Xingcheng)Ministry of Agriculture Beijing Research center for agricultural Standards and testing Zhengzhou Institute of Pomology Chinese Academy of Agricultural Sciences Institute of quality Standards & testing Technology for Agro-products Xinjiang Academy of Agricultural Sciences Yantai Academy of agricultural Sciences Northwest A&F University Institute of Tianjin Agriculture quality Standard and testing Technology

The presence of pesticide residues in pears is a serious health concern. This study presents the results from a 2-year investigation （2013-2014） that used gas chromatography, GS/MS and UPLC/MS-MS to measure the levels of 104 pesti- cides in 310 pear samples. In 93.2% of the samples, 43 pesticides were detected, of which the maximum residue levels （MRLs） were exceeded in 2.6% of the samples. Multiple residues （two to eight compounds） were present in 69.7% of the samples; one sample contained nine pesticides and one sample contained 10. Only 6.8% of the samples did not contain residues. To assess the health risks, the pesticide residue data have been combined with daily pear consumption data for children and adult populations. A deterministic model was used to assess the chronic and acute exposures based on the Joint Meeting on Pesticide Residues （JMPR） method. A potential acute risk was demonstrated for children in the case of bifenthrin, which was found to be present at 105.36% of the acute reference dose （ARfD） value. The long- term exposure of the Chinese consumer to pesticide residues through the consumption of raw pears was far below the acceptable daily intake （ADI） criterion. Additionally, the matrix ranking scheme was used to classify risk subgroups of pesticides and pear samples. In general, 95.5% of samples were deemed to be safe and nine pesticides were classified as being of a relatively high risk. The findings indicated that the occurrence of pesticide residues in pears should not be considered a serious public health problem. Nevertheless, a more detailed study is required for vulnerable consumer groups, especially children. Continuous monitoring of pesticides in pears and tighter regulation of pesticide residue standards are recommended.

关键词： pear pesticide residue risk assessment intake risk ranking

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Correlation of Aflatoxin Contamination in Peanuts with Rhizosphere Microorganisms and Its Comprehensive Control

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agricultural Biotechnology 2019年第2期8卷 232-234,237页

作者： Suyan GAO Shoumin JIA Litian ZHANG Yi ZHANG Haitao Zhao Aili XIANG Biao QI Lixue DONG Tangshan center for Comprehensive Food and Drug Inspection and testing hebei agricultural products quality and safety testing innovation center

Aflatoxin contamination is an important hazard factor affecting the quality safety and food safety of peanut production, which seriously threatens people's health and safety. In this paper, the background and harmful causes of aflatoxin contamination were analyzed in detail, with attention focusing on its control measures, which has certain guiding significance for the comprehensive control of aflatoxin contamination.

关键词： Peanut Aflatoxin contamination Comprehensive control measures

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Temporal-spatial analysis of cyromazine in cowpea using liquid chromatography–mass spectrometry coupled with mass spectrometry imaging

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Food quality and safety 2023年第3期7卷 466-473页

作者： Xuemei Tang Congling Huang Jianxiang Huang Jiale Wang Zhiting Chen Yan Chen Zemiao Xiao Kai Wan Institute of quality Standard and Monitoring Technology for Agro-products of Guangdong Academy of agricultural Sciences Key Laboratory of Testing and Evaluation for Agro-Product Safety and Quality(Guangzhou)Ministry of Agriculture and Rural AffairsNational Reference Laboratory for Agricultural Testing(Pesticide Residues)GuangzhouChina

Background:Cowpea(Vigna unguiculata ***.)is an economically important *** is nutritious and popular with ***,it has been listed as one of the agricultural products of critical concern about safety in China and cyromazine is the major risk ***:This study analyzed the dissipation and permeation kinetics of cyromazine residue in cowpea,to offer a scientific basis for the rational use of pesticide and ensure the safety of agricultural *** and methods:The dissipation and residue level of the systemic insecticide cyromazine on cowpea under field and stored conditions were ***,the spatial distribution of cyromazine was studied using mass spectrometry imaging to visualize the dynamic processes of permeation and migration in the tissues post pesticide ***:The dissipation processing of cyromazine in cowpea was shown to follow the first-order kinetics and half-life was 7.76 d in the *** cowpea,the permeation and migration rate of cyromazine was faster than that in kidney beans and accumulation was mainly in the *** is not safe to apply cyromazine to cowpeas with reference to the application method on kidney ***:These findings present vital data for the determination of risks linked with cowpea consumption and pesticide intake.

关键词： Cyromazine cowpea mass spectrometry imaging pesticide residue

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Determination of Amantadine Residue in Honey by Solid-phase Extraction and High-performance Liquid Chromatography with Pre-column Derivatization and Fluorometric Detection

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Chinese Journal of Chemistry 2011年第8期29卷 1764-1768页

作者：张金振赵静周金慧薛晓峰李熠吴黎明陈芳 Bee products quality Supervision and testing center Institute of Apicultural Research Chinese Academy of Agricultural Sciences Beijing 100093 China

Amantadine （AMA） is an anti-viral drug used in apiculture to protect honeybee against the sacbrood virus （Morator aetatulae）. This study described a reliable high-performance liquid chromatographic （HPLC） method for analyzing AMA in honey using a solid-phase extraction （SPE） cartridge （Plexa PCX） for purification, 4-fluoro-7- nitro-2,1,3-benzoxadiazole （NBD-F） as a pre-column derivatization agent, and fluorometric detection （λex =470 nm, λem=530 nm）. The chromatographic separation was performed on an XDB C18 column （150×4.6 mm i.d.） using 0.1% trifluoroacetic acid/acetonitrile （35 ; 65, V/V） as the mobile phase at a flow rate of 1.0 mLomin 1 with a run time of 20 min. Under these optimal conditions, a linear relationship was observed in the range of 0.025--1.0μg·mL-1 with a good correlation coefficient （0.998） and low limit of detection （0.0080 μg·g-1）, the recoveries were all above 90%, and the intra-day and inter-day precision （RSD） ranged from 3.4%--5.1%.

关键词： amantadine honey solid-phase extraction high-performance liquid chromatographic (HPLC),fluorometric detection

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Time effects of rice straw and engineered bacteria on reduction of exogenous Cu mobility in three typical Chinese soils

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Pedosphere 2022年第5期32卷 665-672页

作者： Hanzhi SHI Dian WEN Yongdong HUANG Shoujun XU Tenghaobo DENG Furong LI Zhichao WU Xu WANG Peihua ZHAO Fuhua WANG Ruiying DU Institute of quality Standard and Monitoring Technology for Agro-products Guangdong Academy of Agricultural SciencesGuangzhou 510640(China) Key Laboratory of testing and Evaluation for Agro-Product safety and quality Ministry of Agriculture and Rural AffairsGuangzhou 510640(China) Guangdong Provincial Key Laboratory of quality&safety Risk Assessment for Agro-products Guangzhou 510640(China)

Globally,copper(Cu)accumulation in soils is a major environmental *** organic waste and some bacterial species can readily absorb metals in an eco-friendly manner,and thus are commonly used in metal-contaminated soil *** study investigates the change in Cu fractions during the aging process and the time effects of rice straw(RS)and engineered bacteria(EB)(Pseudomonas putida X4/pIME)on reduction of Cu *** typical Chinese soils(red,cinnamon,and black soils)were incubated with RS or RS+EB in the presence of exogenous Cu for 24 *** soil physicochemical properties,reactive soil components,Cu fractions,and Cu mobility were determined over *** Cu mobility factor(MF)values were the lowest in the black soil(6.4-9.2)because of its high organic carbon and clay *** additions of both RS and RS+EB accelerated Cu stabilization during the aging process in all three *** Cu MF values decreased with time during the initial 20 months;however,the MF values increased thereafter in all soils,which might be due to the reduction of humic substances and amorphous iron oxides and the increase in iron oxides complexed on the organic *** reduction rates of Cu MF were similar after 16,24,and more than 24 months in the red,cinnamon,and black soils,respectively,indicating that RS and RS+EB could limit Cu mobility at different times in various *** RS treatment showed the greatest efficiency in reducing Cu mobility in the red,cinnamon,and black soils after 12,12,and 8 months of incubation,*** RS+EB treatment was more efficient than the RS treatment in the red soil during the initial 8 months of the incubation *** study provides theoretical support for Cu risk assessments and RS supplementation for Cu remediation in different soils.

关键词： aging Cu distribution mobility factor sequential extraction soil incubation

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Graphene oxide influences bacterial community and soil environments of Cd-polluted Haplic Cambisols in Northeast China

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Journal of Forestry Research 2021年第4期32卷 1699-1711页

作者： Jiaxin Ru Guoyou Chen Yong Liu Ying Sang Jinfeng Song Key Laboratory of Sustainable Forest Ecosystem Management-Ministry of Education School of ForestryNortheast Forestry UniversityHarbin 150040HeilongjiangPeople’s Republic of China Inspection and testing center for quality of Cereals and their products-Ministry of Agriculture and Rural Aff airs Quality and Safety Institute of Agricultural ProductsHeilongjiang Academy of Agricultural SciencesHarbin 150086HeilongjiangPeople’s Republic of China Xinghua products quality Inspection and testing center No.4 Zhonghe RoadTaizhou 225700JiangsuPeople’s Republic of China

Graphene oxide(GO),a carbon nanomaterial that is widely used in the environment and other industries,may pose potential risks to ecosystems,especially the soil *** soils in Northeast China are frequently polluted with cadmium(Cd) ***,there is no study on the influence of GO on the Cd-contaminated soil microbial community and soil chemical *** this study,Cd(100 mg kg^(-1))-polluted soils were treated with different concentrations of GO(0,25,50,150,250,and 500 mg L^(-1),expressed as T1,T2,T3,T4,T5,and T6,respectively) for 40 *** treatment without Cd pollution and GO served as the control(CK).Then,we investigated the influence of the GO concentrations on the bacterial community and chemical properties of Cd-polluted Haplic Cambisols,the zonal soil in Northeast *** GO addition,the richness and diversity indexes of the bacterial community in Cd-contaminated Haplic Cambisols initially increased by 0.05-33.92% at 25 mg L^(-1),then decreased by0.07-2.37% at 50 mg L^(-1),and then increased by 0.01-24.37%within 500 mg L^(-1) *** species and abundance of bacteria varied with GO concentration,and GO significantly increased bacterial growth at 25 and 250 mg L^(-1).GO treatments influenced the bacterial community structure,and the order of similarity of the bacterial community structure was as follows:T4=T5> T1=T6> T2> T3> *** and Acidobacteria were the dominant bacteria,accounting for 36.0% and 26.2%,respectively,of soil *** GO treatments also significantly affected the metabolic function of bacteria and further influenced the diversity of the bacterial community structure by affecting several key soil chemical properties:soil pH,organic matter and available potassium,phosphorus,and *** results provide a theoretical basis for scientific and comprehensive evaluation of the environmental impacts of GO on the zonal forest soils of Northeast China.

关键词： Cadmium pollution Haplic cambisols Graphene oxide Bacterial community Soil environmental factors

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Derivation of soil Pb/Cd/As thresholds for safety of vegetable planting: A case study for pakchoi in Guangdong Province,China

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Journal of Integrative Agriculture 2019年第1期18卷 179-189页

作者： LI Fu-rong WEN Dian WANG Fu-hua SUN Fang-fang WANG Xu DU Ying-qiong LIU Xiang-xiang WAN Kai Public Monitoring center for Agro-product of Guangdong Academy of agricultural Sciences/Key Laboratory of testing and Evaluation for Agro-product safety and quality Ministry of Agriculture/Laboratory of Quality & Safety Risk Assessment for Agro-product (Guangzhou)Ministry of Agriculture

Regional guidelines for soil heavy metals,in conjunction with specific crops,are proposed to better assess soil environmental *** this study,large amounts of soils with different levels of Pb/Cd/As pollution were collected in Guangdong Province,China,to carry out a pot experiment,which was closer to the actual situation and properties of the heavy metal contaminated *** the widely planted vegetable pakchoi as the research object,we analyzed the correlations between the Pb/Cd/As concentrations in pakchoi and the total or available soil Pb/Cd/As concentrations and established their optimal regression *** then,the total and available soil Pb/Cd/As thresholds for pakchoi were calculated according to the allowable Pb/Cd/As limits in pakchoi and were compared with the current soil assessment ***,a lot of paired vegetable-soil samples in field were collected and their pollution situations were assessed by both the current limit thresholds and the calculated ***,it was more consistent to assess the Cd and As pollution situation of the vegetable and soil samples with the calculated soil thresholds than the current soil assessment *** further proved that it was necessary to explore the soil heavy metal thresholds for safety of vegetable in specific regions,which would be more scientific and practical to guide safety production of local agricultural products and effective utilization of soil resources.

关键词： heavy metal pollution available content soil threshold correlation analysis vegetable

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In-package cold plasma treatment of braised chicken: voltage effect

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Food Science and Human Wellness 2022年第4期11卷 845-853页

作者： Yali Zhang Yang Lei Suhong Huang Xiaoli Dong Jichao Huang Ming Huang Nanjing innovation center of Meat products Processing Jiangsu Collaborative Innovation Center of Meat Production and ProcessingQuality and Safety Controland College of Food Science and TechnologyNanjing Agricultural UniversityNanjing 210095China College of Engineering Nanjing Agricultural UniversityNanjing 210095China

This study investigated the effect of different dielectric barrier discharge cold plasma(DBD-CP) treatment voltages on the microbiological growth and quality characteristics of braised chicken during storage at(4 ± 1) ℃. Argon-packed samples were subjected to DBD-CP treatment for 3 min at voltages of 50, 60 and 70 k V. As a result, there was no significant(P > 0.05) difference in L*, a*, b* and p H of braised chicken between control and treatment groups at the same storage time. However, the development of lipid oxidation in DBD-CP treatment samples was slower than that in air-packed samples. Compared to air-packed samples, the reduction of total viable count, yeasts and molds, lactic acid bacteria and Pseudomonads spp. in 70 k V treated samples on day 15 were 3.21, 2.41, 2.44 and 1.96 lg(CFU/g), respectively. The results indicate that DBD-CP treatment could extend the shelf life of braised chicken without negative impacts on quality characteristics.

关键词： In-package cold plasma Braised chicken Shelf-life quality

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Changes in fruit pigment accumulation,chloroplast development,and transcriptome analysis in the CRISPR/Cas9-mediated knockout of Stay-green 1(slsgr1)mutant

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Food quality and safety 2022年第1期6卷 3-12页

作者： Liqun Ma Ni Zeng Ke Cheng Jinyan Li Keru Wang Chunjiao Zhang Hongliang Zhu College of Food Science and Nutritional Engineering China Agricultural UniversityBeijingChina Supervision Inspection&Testing Center of Agricultural Products QualityMinistry of Agriculture and Rural AffairsBeijingChina

The green-flesh(gf)mutant of the tomato fruit ripen to a muddy brown color and has been demonstrated previously to be a loss-of-function ***,we provide more evidence to support this view that S/SGR1 is involved in color change in ripening tomato *** out SISGR1 expression using a clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9 genome editing strategy showed obviously a muddy brown color with significantly higher chlorophyll and carotenoid content compared with wild-type(WT)*** further verify the role of SiSGR1 in fruit color change,we performed transcriptome deep sequencing(RNA-seq)analysis,where a total of 354 differentially expressed genes(124/230 downregulated/upregulated)were identified between WT and ***,the expression of numerous genes associated with photosynthesis and chloroplast function changed significantly when S/SGR1 was knocked *** together,these results indicate that S/SGR1 is involved in color change in ripening fruit via chlorophyll degradation and carotenoid biosynthesis.

关键词： Tomato pigmentaccumulation transcriptome analysis

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Integrative metabolomics and transcriptomics analyses reveal pivotal regulatory mechanisms of 1-methylcyclopropene in maintaining postharvest storage quality of‘Fuji’apples

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Food quality and safety 2023年第1期7卷 49-62页

作者： Lingling Zhang Peiyu Zhang Modi Gao Yi Zhao Chunjiao Zhang Hongliang Zhu College of Food Science and Nutritional Engineering China Agricultural UniversityBeijingChina Supervision Inspection&Testing Center of Agricultural Products QualityMinistry of Agriculture and Rural AffairsBeijingChina

In this study,integrative metabolomics and transcriptomics analyses were conducted to investigate the effects of 1-methylcyclopropene(1-MCP)on apple fruit quality during long-term cold *** results showed that 1-MCP(1μL/L)treatment could maintain fruits apparent quality(*** color and firmness),inhibit the increase of rot rate and soluble solids content/titratable acidity ratio,decrease ethylene release,and respiratory intensity during cold storage,and extend shelf ***,1-MCP had long-term effects on the accumulation of many qualities related to metabolite and gene expression in fruits.1-MCP affected genes related to metabolism at the early stage of storage,specifically those of the glycolysis and tricarboxylic acid cycle *** related to the degradation of sucrose,starch,and cellulose were inhibited,and some starch and cellulose synthesis genes were up-regulated by *** from ethylene synthesis and signal transduction being inhibited by 1-MCP,several enzymes(pectinesterase,pectate lyase,polygalacturonase)were involved in pectin degradation,and degradation products of the cell wall(i.e.D-galacturonic acid and D-glucuronic acid)were also strongly inhibited,further maintaining fruit ***,as precursor glutathione(GSH)related to plant resistance,up-regulated the synthase ***,the expression of genes related to cyanoalanine syn-thase and amino acid utilization pathways was suppressed by 1-MCP Collectively,1-MCP could maintain the postharvest quality of apple fruits.

关键词： 1-Methylcyclopropene apple fruit quality metabolomics transcriptomics

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