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Chinese Journal of Oceanology and Limnology 2009年第1期27卷 74-79页

作者：李莉郭希明张国范 Key laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences haskin shellfish research laboratory Institute of Coastal and Marine Sciences Rutgers University

Microsatellites were screened in a backcross family of the Pacific oyster, Crassostrea gigas. Fifteen microsatellite loci were distinguishable and polymorphic with 6 types of allele-combinations. Null alleles were detected in 46.7% of loci, accounting for 11.7% of the total alleles. Four loci did not segregate in Mendelian Ratios. Three linkage groups were identified among 7 of the 15 segregating loci. Fluorescence-based automated capillary electrophoresis (ABI 310 Genetic Analyzer) that used to detect the microsatellite loci, has been proved a fast, precise, and reliable method in microsatellite genotyping.

关键词： Crassostrea gigas microsatellite null allele genetic mapping

Preliminary genetic linkage map of the abalone Haliotis diversicolor Reeve

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Chinese Journal of Oceanology and Limnology 2010年第3期28卷 549-557页

作者：石耀华郭希明顾志峰王爱民王嫣 Ocean College Hainan University Key Laboratory of Tropic Biological Resources MOE Hainan Key Laboratory of Tropical Hydrobiological Technology haskin shellfish research laboratory Institute of Marine and Coastal Sciences Rutgers University

Haliotis diversicolor Reeve is one of the most important mollusks cultured in South China. Preliminary genetic linkage maps were constructed with amplified fragment length polymorphism (AFLP) markers. A total of 2 596 AFLP markers were obtained from 28 primer combinations in two parents and 78 offsprings. Among them, 412 markers (15.9%) were polymorphic and segregated in the mapping family. Chi-square tests showed that 151 (84.4%) markers segregated according to the expected 1:1 Mendelian ratio (P<0.05) in the female parent, and 200 (85.8%) in the male parent. For the female map, 179 markers were used for linkage analysis and 90 markers were assigned to 17 linkage groups with an average interval length of 25.7 cm. For the male map, 233 markers were used and 94 were mapped into 18 linkage groups, with an average interval of 25.0 cm. The estimated genome length was 2 773.0 cm for the female and 2 817.1 cm for the male map. The observed length of the linkage map was 1 875.2 cm and 1 896.5 cm for the female and male maps, respectively. When doublets were considered, the map length increased to 2 152.8 cm for the female and 2 032.7 cm for the male map, corresponding to genome coverage of 77.6% and 72.2%, respectively.

关键词： amplification fragment length polymorphism (AFLP) genetic linkage map Haliotis diversicolor Reeve

TETRAPLOID INDUCTION BY INHIBITING MITOSIS I IN SCALLOP CHLAMYS FARRERI

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Chinese Journal of Oceanology and Limnology 1999年第4期17卷 350-358,344页

作者：杨蕙萍郭希明陈再忠王永平 Experiment Marine Biology laboratory Institute of Oceanology Chinese Academy of Sciences Qingdao China haskin shellfish research laboratory Rutgers University Port Norris USA Fisheries College Ocean University of Qingdao Qingdao China

Tetraploid induction was carried out by inhibiting mitosis I in fertilized eggs ofChlamys farreri. Mitosis I was blocked with cold shock (5–7°C), Cytochalasin B (0.75 mg/L) and 6-dimethylaminopurine (6-DMAP) (60–75 mg/L) when 60% fertilized eggs released polar body II at 20°C. At 4-cells embryo stage, the ploidy was determined by counting chromosome number. In control groups, most embryos were diploids (72.22%) and aneuploids (24.78%). In Cytochalasin B, cold shock and 6-DMAP treated groups, tetraploids were respectively 10.51%, 4.08%, and 13.34%; aneuploids were 43.10%, 35.93% and 29.16%, and triploids were 7.84%, 8.52% and 18.33%. At D-larva stage, ploidy was determined by flow cytometry (FCM). The ploidy analysis of day 2 larvae showed diploids in control group and also in three treated groups. Juvenile scallops (0.2–0.3cm) which were harvested in two control groups and two CB treated groups were all diploids through checking ploidy individually by FCM.

关键词： scallopChalamys farreri tetraploid Cytochalasin B mitosis I

The potential of ocean acidification on suppressing larval development in the Pacific oyster Crassostrea gigas and blood cockle Area inflata Reeve

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Chinese Journal of Oceanology and Limnology 2014年第6期32卷 1307-1313页

作者：李加琦蒋增杰张继红毛玉泽卞大鹏方建光 Mariculture Ecology and Carrying Capacity laboratory Yellow Sea Fisheries Research InstituteChinese Academy of Fisheries Sciences National Engineering research Center of Marine shellfish

We evaluated the effect of pH on larval development in larval Pacific oyster(Crassostrea gigas) and blood cockle(Arca inflata Reeve).The larvae were reared at pH 8.2(control),7.9,7.6,or 7.3beginning 30 min or 24 h post *** to lower pH during early embryonic development inhibited larval shell formation in both *** with the control,larvae took longer to reach the D-veliger stage when reared under pH 7.6 and *** to lower pH immediately after fertilization resulted in significantly delayed shell formation in the Pacific oyster larvae at pH 7.3 and blood cockle larvae at pH 7.6 and ***,when exposure was delayed until 24 h post fertilization,shell formation was only inhibited in blood cockle larvae reared at pH ***,the early embryonic stages were more sensitive to acidified *** results suggest that ocean acidification will have an adverse effect on embryonic development in *** the effects appear subtle,they may accumulate and lead to subsequent issues during later larval development.

关键词： ocean acidification larval development bivalve calcifier

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中国北方沿海“褶牡蛎”的分类和订名

海洋科学 2009年第10期33卷 104-106页

作者：王海艳郭希明刘晓许飞张素萍徐凤山张国范中国科学院海洋研究所山东青岛266071 haskin shellfish research laboratory Rutgers University

中国牡蛎的种类繁多,多样性极为丰富,由于其形态变异大,已报道的中国牡蛎物种组成及分布争议甚大,中国北方沿海常见"褶牡蛎"的分类也一直存在很大的争议,这严重阻碍了其他相关研究的进行。作者系统综述了中国北方沿海"褶牡蛎"的分类历... 详细信息

中国牡蛎的种类繁多,多样性极为丰富,由于其形态变异大,已报道的中国牡蛎物种组成及分布争议甚大,中国北方沿海常见"褶牡蛎"的分类也一直存在很大的争议,这严重阻碍了其他相关研究的进行。作者系统综述了中国北方沿海"褶牡蛎"的分类历史和研究现状,并对中国北方沿海"褶牡蛎"进行了系统的分类研究,取得了最新研究成果。确立了中国北方沿海"褶牡蛎"的分类地位,改变其分类长期混乱的局面,这对于"褶牡蛎"的养殖和其他相关研究均具有重要的意义。

关键词： “褶牡蛎” 分类订名

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中国近海“近江牡蛎”的分类和订名

海洋科学 2007年第9期31卷 85-86页

作者：王海艳郭希明刘晓张国范张素萍徐凤山中国科学院海洋研究所山东青岛266071 haskin shellfish research laboratory Rutgers University

牡蛎（Ostreidae）属软体动物门（Mollusca）、双壳纲（Bivalvia）、珍珠贝目（Pterioidae），为世界性广分布种类。由于其肉味鲜美，营养丰富，从远古时代就被人类所食用，具有很高的经济价值，是世界各国海水养殖业重要的养殖对象，... 详细信息

牡蛎（Ostreidae）属软体动物门（Mollusca）、双壳纲（Bivalvia）、珍珠贝目（Pterioidae），为世界性广分布种类。由于其肉味鲜美，营养丰富，从远古时代就被人类所食用，具有很高的经济价值，是世界各国海水养殖业重要的养殖对象，是目前中国乃至世界产量最大的经济贝类。

关键词：近江牡蛎中国近海分类软体动物门海水养殖业世界性营养丰富远古时代

Seawater acidification affects the immune enzyme activities of the Manila clam Ruditapes philippinarum

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Journal of Oceanology and Limnology 2018年第5期36卷 1688-1696页

作者： HUO Zhongming MENG Xiangyu Md.Golam RBBANI CAO Weinan WU Qidi LI Ying WANG Jingtian YUAN Hongmei YANG Feng YAN Xiwu Engineering research Center of shellfish Culture and Breeding in Liaoning Province College of Fisheries and Life Science Dalian Ocean UniversityDalian 116023China

Ocean acidification leads to changes in physiological and immune responses of bivalves, but the eff ect on the immune enzyme activities of the Manila clam, R uditapes philippinarum, when the pH is lower than the normal value has not been studied in detail. In this study, experiments were conducted to determine how pH(7.4, 7.7, 8.0) affects the immune enzyme activities in the gill and hemocytes of the Manila clam. Membrane stability and phagocytosis increased with decrease of pH from 8.0 to 7.7 and then decreased at pH 7.4. The total protein content in the hemocytes and gills decreased with decreasing pH. Lysozyme content in the hemocytes increased with decreasing pH, and the diff erences were significant among the different pH groups(P research investigating exposure of bivalves to elevated CO_2 conditions.

关键词： seawater acidification immune enzyme Ruditapes philippinarum

Dietary cholesterol promotes growth and ecdysone signalling pathway by modulating cholesterol transport in swimming crabs(Portunus trituberculatus)

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Animal Nutrition 2022年第3期10卷 249-260页

作者： Tingting Zhu Qicun Zhou Zheng Yang Yingying Zhang Jiaxiang Luo Xiangsheng Zhang Yuedong Shen Lefei Jiao Douglas R.Tocher Min Jin laboratory of Fish and shellfish Nutrition School of Marine SciencesNingbo University315211 NingboChina Guangdong Provincial Key laboratory of Marine Biotechnology Institute of Marine SciencesShantou University515063 ShantouChina

Cholesterol,as an indispensable nutrient,regulates molting and growth in *** crustaceans are unable to biosynthesize cholesterol de novo,it is central to understand how dietary cholesterol affects molting in *** 8-week feeding trial was conducted to evaluate the effects of dietary cholesterol level(0.12%,0.43%,0.79%,1.00%,1.30%and 2.50%)on growth,cholesterol metabolism and expression of genes related to lipid and ecdysone metabolism in female swimming crabs(Portunus trituberculatus).A total of 192 crabs(1.41±0.05 g)were randomly distributed into 192 *** treatment had 4 replicates with each replicate containing 8 *** fed the 1.00%cholesterol diet showed best growth performance,and thus based on percent weight gain,the optimal dietary cholesterol requirement was calculated at 1.01%.Tissue cholesterol concentrations were positively correlated with dietary cholesterol *** contents of functional fatty acids in hepatopancreas significantly increased as dietary cholesterol increased from 0.12%to 2.50%(P<0.05).The expression levels of genes related to lipogenesis pathway,lipid catabolism and fatty acid oxidation were significantly downregulated with increased dietary cholesterol level(P<0.05).The highest expression levels of cholesterol transport genes,low-density lipoprotein receptor(ldlr)and low-density lipoprotein receptorrelated protein 2(lrp2)occurred in crabs fed the 1.30%cholesterol ***,hormones related to molting such as crustacean hyperglycemic hormone(CHH),methyl farnesoate(MF),molt-inhibiting hormone(MIH),and ecdysone in hemolymph were significantly influenced by dietary cholesterol level(P<0.05).The highest expression levels of ecdysone receptor(ecr)and chitinase 1(chi1)in eyestalk and hepatopancreas were found in crabs fed the diet containing 1.00%cholesterol(P<0.05).In conclusion,the optimal dietary level was beneficial to functional fatty acid accumulation,regulated lipid metabolism,promoted the ecdysone s

关键词： Portunus trituberculatus Cholesterol Lipid metabolism Ecdysone signalling pathway

High dietary lipid level promotes low salinity adaptation in the marine euryhaline crab(Scylla paramamosain)

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Animal Nutrition 2023年第1期12卷 297-307页

作者： Jiaxiang Luo Chen Ren Tingting Zhu Chen Guo Shichao Xie Yingying Zhang Zheng Yang Wenli Zhao Xiangsheng Zhang Jingjing Lu Lefei Jiao Qicun Zhou Douglas R.Tocher Min Jin laboratory of Fish and shellfish Nutrition School of Marine SciencesNingbo UniversityNingbo 315211China Guangdong Provincial Key laboratory of Marine Biotechnology Institute of Marine SciencesShantou UniversityShantou 515063China

The physiological processes involved in adaptation to osmotic pressure in euryhaline crustaceans are highly energy demanding,but the effects of dietary lipids(fat)on low salinity adaptations have not been well *** the present study,a total of 120 mud crabs(Scylla paramamosain,BW=17.87±1.49 g)were fed control and high-fat(HF)diets,at both medium salinity(23‰)and low salinity(4‰)for 6 wk,and each treatment had 3 replicates with each replicate containing 10 *** results indicated that a HF diet significantly mitigated the reduction in survival rate,percent weight gain and feed efficiency induced by low salinity(P<0.05).Low salinity lowered lipogenesis and activated lipolysis resulting in lipid depletion in the hepatopancreas of mud crabs(P<0.05).Thus,HF diets enhanced the process of lipolysis to supply more *** the gills,low salinity and the HF diet increased the levels of mitochondrial biogenesis markers,the activity of mitochondrial complexes,and the expression levels of genes related to energy metabolism(P<0.05).Consequently,the positive effects of the HF diet on energy metabolism in mud crabs at low salinity promoted osmotic pressure ***,significantly higher haemolymph osmotic pressure and inorganic ion content,as well as higher osmotic pressure regulatory enzyme activity in gills,and gene and protein expression levels of NaK-ATPase were observed in crabs fed the HF diet at low salinity(P<0.05).In summary,high dietary lipid levels improved energy provision to facilitate mitochondrial biogenesis,which increased ATP provision for osmotic pressure regulation of mud *** study also illustrates the importance of dietary lipid nutrition supplementation for low salinity adaptations in mud crabs.

关键词： Osmotic pressure regulation Mitochondrial energy metabolism Lipid metabolism

Dietary choline activates the Ampk/Srebp signaling pathway and decreases lipid levels in Pacific white shrimp(Litopenaeus vannamei)

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Animal Nutrition 2023年第4期15卷 58-70页

作者： Jingjing Lu Xinyue Tao Jiaxiang Luo Tingting Zhu Lefei Jiao Peng Sun Qicun Zhou Douglas R.Tocher Min Jin laboratory of Fish and shellfish Nutrition School of Marine SciencesNingbo UniversityNingbo 315211China Guangdong Provincial Key laboratory of Marine Biotechnology Institute of Marine SciencesShantou UniversityShantou 515063China

An 8-week feeding trial was conducted in Pacific white shrimp(Litopenaeus vannamei)to evaluate the effects of dietary choline supplementation on choline transport and metabolism,hepatopancreas histological structure and fatty acid profile,and regulation of lipid *** isonitrogenous and isolipidic diets were formulated to contain different choline levels of 2.91(basal diet),3.85,4.67,6.55,10.70 and 18.90 g/kg,respectively.A total of 960 shrimp(initial weight,1.38±0.01 g)were distributed randomly into twenty-four 250-L cylindrical fiber-glass tanks,with each diet assigned randomly to 4replicate *** results indicated that dietary choline significantly promoted the deposition of choline,betaine and carnitine(P<0.05).The diameters and areas of R cells,total lipid and triglyceride contents in hepatopancreas,and triglyceride and non-esterified fatty acid contents in hemolymph were negatively correlated with dietary choline *** contents of functional fatty acids in hepatopancreas,the activity of acetyl-CoA carboxylase(Acc),and the mRNA expression of fas,srebp and acc were highest in shrimp fed the diet containing 4.67 g/kg choline,and significantly higher than those fed the diet containing 2.91 g/kg,the lowest level of choline(P<0.05).The number of R cells,content of very lowdensity lipoprotein(VLDL),activities of carnitine palmitoyl-transferase(Cpt1),lipoprotein lipase and hepatic lipase,and the mRNA expression levels of cpt1,fabp,fatp,ldlr,and ampk in hepatopancreas increased significantly as dietary choline increased(P<0.05).In addition,hepatopancreas m RNA expression levels of ctl1,ctl2,oct1,badh,bhmt,ck,cept,and cct were generally up-regulated as dietary choline level increased(P<0.01).In conclusion,dietary choline promoted the deposition of choline and its metabolites by up-regulating genes related to choline transport and ***,appropriate dietary choline level promoted the development of hepatopancreas R cells and maintained the n

关键词： Pacific white shrimp Choline Lipid Fatty acid Histology Ampk signaling pathway