检索结果-南通市图书馆

Journal of Integrative Agriculture 2012年第3期11卷 474-482页

作者： YE Jing-jun LI Jian-rong HAN Xiao-xiang ZHANG Lei JIANG Tian-jia XIA Miao co.lege of Food Science and biotechnology Engineering Zhejiang Gongshang University Hangzhou 310035 P.R.China co.lege of Chemistry Chemical Engineering and Food Safety Bohai University Jinzhou 121013 P.R.China

The effects of active modified atmosphere packaging （MAP） on the postharvest quality of shiitake mushrooms stored at co.d temperature （4°C） were *** gas co.ponents were 2% O2 ＋7% co. （MAP1）,2% O2 ＋10% co. （MAP2） and 2% O2 ＋13% co. （MAP3）,*** results showed that active MAP co.ld extend the shelf-life of shiitake mushrooms to 17 d and the co.centration of carbon dioxide co.ld influence the postharvest quality of shiitake ***2 treatment inhibited the increase in respiration rate and malondinaldehyde （MDA） co.tents,delayed the decrease in firmness,soluble sugar and vitamin C,and obviously reduced the activity of polyphenol oxidase （PPO） and the degree of browning,therefore maintaining better quality.

关键词： shiitake mushrooms modified atmosphere package (MAP) postharvest quality

Isolation and Growth Characteristics of SARS-co.-2 in Vero Cell

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2020年第3期35卷 348-350页

作者： Pingping Yao Yachun Zhang Yisheng Sun Yulin Gu Fang Xu Bo Su Chen Chen Hangjing Lu Dehui Wang Zhangnv Yang Biao Niu Jiancai Chen Lixia Xie Lei Chen Yajing Zhang Hui Wang Yuying Zhao Yue Guo Juncheng Ruan Zhiyong Zhu Zhenfang Fu Dayong Tian Qi An Jianmin Jiang Hanping Zhu Key Lab of Vaccine Prevention and Control of Infectious Disease of Zhejiang ProvinceZhejiang Provincial Center for Disease Control and PreventionHangzhou 310051China R&D Department Shanghai King-Cell Biotechnology Co.Ltd.Shanghai 201506China State Key Laboratory of Agricultural Microbiology Huazhong Agricultural UniversityWuhan 430070China co.lege of Veterinary Medicine Huazhong Agricultural UniversityWuhan 430070China

Dear Editors,The co.onavirus disease 2019(co.ID-19),caused by SARS-co.-2,broke out in early December 2019 has escalated into a global pandemic(Lai et al.2020).Till the May 20 th 2020,more than 4,700,000 people were in... 详细信息

关键词： al. infected Cell

A Simple Synthesis of Tetraamino-[60]Fullerene Epoxides as Potential Antitumor Drug Candidates

在线全文

学校读者我要写书评

暂无评论

CCS Chemistry 2024年第10期6卷 2392-2399页

作者： Yicheng Lu Jie Li Xiaolong Liu Dan He Libin Yang Wei Zhou Xie Wang Shanshan Chen Shiliang Chen Yating Liu Xin Wang Jiao Li Jiawei Huo Yang Liu Zehu Wang Meihan Liu Yijun Wang Yamin Li Fuwen Zhao Shumu Li Jinchao Wei Jianan Liu Wei Li Chunru Wang Key Laboratory of Molecular Nanostructure and Nanotechnology Institute of ChemistryChinese Academy of SciencesBeijing 100190 University of Chinese Academy of Sciences Beijing 100190 The National Center for Nanoscience and Technology Beijing 100190 Central South University Changsha410082 Hunan hangzhou Institute of Medicine(HIM) Chinese Academy of SciencesHangzhou310092 Zhejiang Cancer Center Beijing Tongren HospitalCapital Medical UniversityBeijing 100069 Technical Institute of Physics and Chemistry Chinese Academy of SciencesBeijing 100190 Beijing FULLCAN biotechnology co. Beijing 100085

Amphiphilic aminated fullerenes have a broad margin of safety and significant antitumor ***,we develop a simple and versatile synthesis strategy for tetraamino-[60]fullerene epoxide(C_(60)(NR^(1)R^(2))_(4)O)using C_(60)Cl_(6)as a precursor,which notably reduces the reaction time to less than 1 h while retaining a high yield of over 80%with both cyclic and linear seco.dary amine substrates even at the gram *** molecular structure of C_(60)(NR^(1)R^(2))_(4)O is first validated by single-crystal diffraction,and a two-step reaction mechanism co.prising nucleophilic substitution of Cl and the oxidative elimination of Cl_(2)is proposed based on experimental verification and density functional theory simulation.A set of water-soluble aminated C_(60)(NR^(1)R^(2))_(4)O was prepared in large quantities,and in vitro antitumor evaluation unveiled the critical role that terminal primary amino moieties of C_(60)(NR^(1)R^(2))_(4)O play in their antineoplastic *** work provides an effective synthesis method for aminated C_(60)(NR^(1)R^(2))_(4)O,facilitating the development of fullerene-derived tumor-targeted drugs.

关键词： tetraamino-fullerene epoxides simple synthesis C_(60)Cl_(6) water-soluble aminated fullerene antitumor

Anatomical and Chemical Characteristics of a Rolling Leaf Mutant of Rice and Its Eco.hysiological Properties

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Rice science 2008年第3期15卷 201-208页

作者： BAI Lei DUAN Zhuang-qin WANG Jun-min AN Li-zhe ZHAO Zhi-guang CHEN Kun-ming co.lege of Life Sciences Lanzhou University Lanzhou 730000 China Institute of Crop Sciences College of Agriculture and Biotechnology Zhejiang University Hangzhou 310029 China Institute of Crop and Nuclear Technology Utilization Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China

The anatomical and chemical characteristics of a rolling leaf mutant （rlm） of rice （Oryza sativa L.） and its eco.hysiological properties in photosynthesis and apoplastic transport were investigated. co.pared with the wild type （WT）, the areas of whole vascular bundles and xylem as well as the ratios of xylem area/whole vascular bundles area and xylem area/phloem area were higher in rim, whereas the area and the width of foliar bulliform cell were lower. The Fourier transform infrared （FTIR） microspectrosco.y spectra of foliar cell walls differed greatly between rim and WT. The rim exhibited lower protein and polysaccharide co.tents of foliar cell walls. An obvious reduction of pectin co.tent was also found in rim by biochemical measurements. Moreover, the rate of photosynthesis was depressed while the co.ductance of stoma and the intercellular co. co.centration were enhanced in rim. The PTS fluorescence, which represents the ability of apoplastic transport, was 11% higher in rim than in WT. These results suggest that the changes in anatomical and chemical characteristics of foliar vascular bundles, such as the reduction of proteins, pectins, and other polysaccharides of foliar cell walls, participate in the leaf rolling mutation, and co.sequently lead to the reduced photosynthetic dynamics and apoplastic transport ability in the mutant.

关键词： rice rolling leaf mutant anatomical characteristics chemical characteristics apoplastic transport eco.hysiological property Fourier transform infrared microspectrosco.y

Y-chromosome evidence for no independent origin of modern human in China

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2001年第11期46卷 935-937页

作者： KE Yuehai SU Bing LI Hongyu CHEN Lifeng QI Chunjian GUO Xinjun HUANG Wei JIN Jianzhong LU Daru JIN Li Institute of Genetics and Morgan-Tan International Center for Life Science Fudan UniversityShanghai 200433China Kunming Institute of Zoology Chinese Academy of SciencesKunming 650031China Human Genetics Center University of Texas-HoustonHouston 77225USA Department of biotechnology Suzhou UniversitySuzhou 215007China TSBIO Science&Technology co. LtdHangzhou 310013China National Human Genome Center at Shanghai Shanghai 201203China

East Asia is one of the few regions in the world where a large number of human fossils have been unearthed. The co.tinuity of hominid fossils in East Asia, particularly in China has been presented as strong evidence supporting an independent origin of modern humans in this area. To search for such evidence of a possible independent origin of modern humans in China, a total of 9988 male individuals were sampled across China. Three Y-chromosome biallelic markers (M89, M130 and YAP), which were located at the non-re-co.binant region of Y-chromosome, were typed among the samples. Our result showed that all the individuals carry a mutation at one of the three loci. The three mutations (M89T, M130T, YAP+) co.lesce to another mutation (M168T), which was originally derived from Africa about 31000 to 79000 years ago. In other words, all Y-chromosome samples from China, with no exception, were originally derived from a lineage of African origin. Hence, we co.clude that even a very minor co.tribution of in situ hominid origin in China cannot be supported by the Y-chromosome evidence.

关键词： Y-chromosome haplotype SNPs modern human origin Chinese.

Orthogroup and phylotranscriptomic analyses identify transcription factors involved in the plant co.d response:Acase study of Arabidopsis BBX29

在线全文

学校读者我要写书评

暂无评论

Plant co.munications 2023年第6期4卷 148-162页

作者： Shuo Wang Yirong Shen Deyin Deng Liangyu Guo Yixian Zhang Yuqi Nie Yunfei Du Xijuan Zhao Xiaoxue Ye Jianqin Huang Huahong Huang Jian-Kang Zhu Wenwu Wu State Key Laboratory of Subtropical Silviculture Zhejiang A&F UniversityLin’an 311300HangzhouChina Institute of Tropical Biosciences and biotechnology Chinese Academy of Tropical Agricultural SciencesHaikou 571101China Institute of Advanced biotechnology and School of Life Sciences Southern University of Science and TechnologyShenzhen 518055China Center for Advanced Bioindustry Technologies Chinese Academy of Agricultural SciencesBeijing 100081China

C-repeat binding factors(CBFs)are well-known transcription factors(TFs)that regulate plant co.d *** sequencing(RNA-seq)data from diverse plant species provide opportunities to identify other TFs involved in the co.d ***,this task is challenging because gene gain and loss has led to an intertwined co.munity of co.orthologs and in-paralogs between and within *** orthogroup(closely related homologs)analysis,we identified 10,549 orthogroups in five representative eudico.s.A phylotranscriptomic analysis of co.d-treated seedlings from eudico.s identified 35 high-co.fidence co.served co.d-responsive transcription factor orthogroups(co.oFos).These 35 co.oFos included the well-known co.d-responsive regulators CBFs,HSFC1,ZAT6/10,and CZF1 among *** used Arabidopsis BBX29 for experimental *** and genetic analyses showed that co.d-induction of BBX29 is CBF-and abscisic acid-independent,and BBX29 is a negative regulator of co.d *** RNA-seq and Cleavage Under Targets and Tagmentation followed by sequencing analyses revealed that BBX29 represses a set of co.d-induced TFs(ZAT12,PRR9,RVE1,MYB96,etc.).Altogether,our analysis yielded a library of eudico. co.oFos and demonstrated that BBX29 is a negative regulator of co.d tolerance in Arabidopsis.

关键词： phylogenomics phylotranscriptomics orthogroup co.d acclimation BBX29 CBF-independent pathway

Identification of New Allele of FLOURY ENDOSPERM2 in White-co.e Endosperm Mutant of Rice

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Rice science 2022年第5期29卷 407-411,I0017,I0018页

作者： BAO Jinsong ZHANG Yu ZHAO Jiajia CHEN Yaling WU Weixun CAO Liyong XU Feifei Key Laboratory of Nuclear Agricultural Sciences of Ministry of Agriculture and Rural Affairs and Zhejiang Province/Institute of Nuclear Agricultural Sciences College of Agriculture and BiotechnologyZhejiang UniversityHangzhou 310058China Hainan Institute of Zhejiang University Yazhou DistrictSanya 572025China Laboratory of Plant Genetic Improvement and biotechnology Jiangxi Normal UniversityNanchang 330000China State Key Laboratory of Rice Biology/Chinese National Center for Rice Improvement China National Rice Research InstituteHangzhou 310006China

Identification of regulatory genes from chalky/floury endosperm mutants is an important approach to understand the mechanism of starch biosynthesis to accelerate rice grain quality improvement.A mutant GM645,identified from 60co.-irradiation of an indica rice Guanglu’ai 4(GLA4),exhibited white-co.e endosperm and altered starch physico.hemical ***,the causal gene responsible for the white-co.e endosperm in GM645 has not been ***,we developed a reco.bined inbred line(RIL,F7)population derived from a cross between GM645 and a japonica rice Tainong 67(TN67)with translucent *** analysis co.bined with next generation sequencing revealed five single nucleotide polymorphisms(SNPs)in four candidate genes that were highly associated with the white-co.e *** them,one base pair deletion in Os04g0645100 resulted in a frame shift mutation after the 983th amino acid(aa),and a premature stop co.on occurred after the other 11 ***,functional annotation revealed that Os04g0645100 was previously characterized as the FLOURY ENDOSPERM2(FLO2)***-length co.ing sequence from Os04g0645100 driven by the maize ubiquitin promoter was transformed into GM645,and seeds from these transgenic plants expressing Os04g0645100 were largely rescued to translucent,indicating that Os04g0645100 was responsible for the whiteco.e endosperm in GM645.

关键词： properties white ERM

Enhanced water solubility, antioxidant activity, and oral absorption of hesperetin by D-α-toco.heryl polyethylene glyco. 1000 succinate and phosphatidylcholine

在线全文

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & biotechnology) 2019年第3期20卷 273-281页

作者： Su-fang GU Li-ying WANG Ying-jie TIAN Zhu-xian ZHOU Jian-bin TANG Xiang-rui LIU Hai-ping JIANG You-qing SHEN Key Laboratory of Biomass Chemical Engineering of Ministry of Education and Center for Bionanoengineering College of Chemical and Biological EngineeringZhejiang UniversityHangzhou 310027China hangzhou Ubao Healthcare Technology co. Ltd.Hangzhou 310012China Department of Medical Onco.ogy the First Affiliated HospitalZhejiang University School of MedicineHangzhou 310006China

Hesperetin,an abundant bioactive co.ponent of citrus fruits,is poorly water-soluble,resulting in low oral *** developed new formulations to improve the water solubility,antioxidant activity,and oral absorption of *** nano-based formulations were developed,namely hesperetin-TPGS(D-α-toco.heryl polyethylene glyco. 1000 succinate)micelles and hesperetin-phosphatidylcholine(PC)*** two formulations were prepared by a simple technique called solvent dispersion,using US Food and Drug Administration(FDA)-approved excipients for *** scanning calorimetry(DSC)and dynamic light scattering(DLS)were used to characterize the formulations’physical *** analysis,cellular antioxidant activity assay,and a pharmaco.inetic study were performed to evaluate the biological properties of these two *** final weight ratios of both hesperetin to TPGS and hesperetin to PC were 1:12 based on their water solubility,which increased to 21.5-and 20.7-fold,*** hesperetin-TPGS micelles had a small particle size of 26.19 nm,whereas the hesperetin-PC co.plexes exhibited a larger particle size of 219.15 *** addition,the cellular antioxidant activity assay indicated that both hesperetin-TPGS micelles and hesperetin-PC co.plexes increased the antioxidant activity of hesperetin to 4.2-and 3.9-fold,***,the in vivo oral absorption study on rats indicated that the micelles and co.plexes significantly increased the peak plasma co.centration(Cmax)from 2.64μg/mL to 20.67 and 33.09μg/mL and also increased the area under the co.centration–time curve of hesperetin after oral administration to 16.2-and 18.0-fold,*** micelles and co.plexes increased the solubility and remarkably improved the in vitro antioxidant activity and in vivo oral absorption of hesperetin,indicating these formulations’potential applications in drugs and healthcare products.

关键词： Hesperetin D-α-Toco.heryl polyethylene glyco. 1000 succinate(TPGS) Phosphatidylcholine Antioxidant activity Oral absorption

Genome sequence of Gossypium anomalum facilitates interspecific introgression breeding

在线全文

学校读者我要写书评

暂无评论

Plant co.munications 2022年第5期3卷 199-213页

作者： Zhenzhen Xu Jiedan Chen Shan Meng Peng Xu Caijiao Zhai Fang Huang Qi Guo Liang Zhao Yonggang Quan Yixin Shangguan Zhuang Meng Tian Wen Ya Zhang Xianggui Zhang Jun Zhao Jianwen Xu Jianguang Liu Jin Gao Wanchao Ni Xianglong Chen Wei Ji Nanyi Wang Xiaoxi Lu Shihong Wang Kai Wang Tianzhen Zhang Xinlian Shen Key Laboratory of co.ton and Rapeseed(Nanjing) Ministry of Agriculture and Rural Affairsthe Institute of Industrial CropsJiangsu Academy of Agricultural SciencesNanjingChina Institute of Crop Science Plant Precision Breeding AcademyZhejiang Provincial Key Laboratory of Crop Genetic ResourcesCollege of Agriculture and BiotechnologyZhejiang UniversityHangzhouChina JOIN HOPE SEEDS co. Ltd.ChangjiChina Key Laboratory of Genetics Breeding and Multiple Utilization of Crops(MOE)Fujian Agriculture and Forestry UniversityFuzhouChina

Crop wild relatives are an important reservoir of natural biodiversity. However, inco.porating wild geneticdiversity into breeding programs is often hampered by reproductive barriers and a lack of accurate genomicinformation. We assembled a high-quality, accurately centromere-anchored genome of Gossypium anomalum, a stress-tolerant wild co.ton species. We provided a strategy to disco.er and transfer agronomicallyvaluable genes from wild diploid species to tetraploid co.ton cultivars. With a (Gossypium hirsutum 3 ***)2 hexaploid as a bridge parent, we developed a set of 74 diploid chromosome segment substitution lines (CSSLs) of the wild co.ton species G. anomalum in the G. hirsutum background. This set of CSSLsincluded 70 homozygous substitutions and four heterozygous substitutions, and it co.lectively co.tainedabout 72.22% of the G. anomalum genome. Twenty-four quantitative trait loci associated with plant height,yield, and fiber qualities were detected on 15 substitution segments. Integrating the reference genome withagronomic trait evaluation of the CSSLs enabled location and cloning of two G. anomalum genes thatenco.e peroxiredoxin and putative callose synthase 8, respectively, co.ferring drought tolerance andimproving fiber strength. We have demonstrated the power of a high-quality wild-species reference genomefor identifying agronomically valuable alleles to facilitate interspecific introgression breeding in crops.

关键词： wild diploid species Gossypium anomalum genome chromosome segment substitution lines drought tolerance fiber strength

Drying temperature affects rice seed vigor via gibberellin,abscisic acid,and antioxidant enzyme metabolism

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & biotechnology) 2020年第10期21卷 796-810页

作者： Yu-tao HUANG Wei WU Wen-xiong ZOU Hua-ping WU Dong-dong CAO institute of Crop and Nuclear Technology Utilization Zhejiang Academy of Agricultural SciencesHangzhou 310021China Seed Management Station of Zhejiang Province Hangzhou 310020China Huzhou Keao Seed co. Ltd.Huzhou 313000China

Seed vigor is a key factor affecti ng seed *** mechanical drying process exerts a sign ificant in fluence on rice seed *** initial moisture co. tent(IMC)and drying temperature are co.sidered the main factors affecting rice seed vigor through mechanical *** study aimed to determine the optimum drying temperature for rice seeds acco.ding to the IMC,and elucidate the mechanisms mediating the effects of drying temperature and IMC on seed *** seeds with three different IMCs(20%,25%,and 30%)were dried to the target moisture co.tent(14%)at four different drying *** results showed that the drying temperature and IMC had significant effects on the drying performance and vigor of the rice *** upper limits of drying temperature for rice seeds with 20%,25%,and 30%IMCs were 45,42,and 38°C,*** drying rate and seed temperature increased sign ificantly with in creasing drying *** drying temperature,drying rate,and seed temperature showed extremely significant negative co.relations with germination energy(GE),germination rate,germination index(Gl),and vigor index(VI).A high IMC and drying temperature probably induced a massive accumulation of hydrogen peroxide(H2O2)and superoxide anions in the seeds,enhanced superoxide dismutase(SOD)and catalase(CAT)activity,and increased the abscisic acid(ABA)*** the early stage of seed germination,the IMC and drying temperature regulated seed germination through the metabolism of H2O2,gibberellin acid(GA),ABA,and *** results indicate that the metabolism of reactive oxygen species(ROS),antioxidant enzymes,GA,ABA,and a-amylase might be involved in the mediation of the effects of drying temperature on seed *** results of this study provide a theoretical basis and technical guidance for the mechanical drying of rice seeds.

关键词： Drying temperature Rice Seed vigor Gibberellin acid(GA) Abscisic acid(ABA) Antioxidant enzyme