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Urinary donor-derived cell-free DNA as a non-invasive biomarker for BK polyomavirus-associated nephropathy

Journal of Zhejiang University-Science B(Biomedicine & biotechnology) 2021年第11期22卷 917-928页

作者： Jia SHEN Luying GUO Wenhua LEI Shuaihui LIU Pengpeng YAN Haitao LIU Jingyi ZHOU Qin ZHOU Feng LIU Tingya JIANG Huiping WANG Jianyong WU Jianghua CHEN Rending WANG Kidney Disease Center the First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310003China National Key Clinical Department of Kidney Diseases Hangzhou 310003China Key Laboratory of Kidney Disease Prevention and co.trol Technology of Zhejiang Province Hangzhou 310003China Zhejiang University Institute of Nephrology Hangzhou 310003China AlloDx(Shanghai)Biotech. Co.Ltd.Shanghai 201100China Organ Donation and co.rdination Office the First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310003China

BK polyomavirus-associated nephropathy(BKPyVAN)is a co.mon cause of allograft ***,differentiation between BKPyVAN and type I T cell-mediated rejection(TCMR)is challenging when simian virus 40(SV40)staining is negative,because of the similarities in *** study investigated whether donor-derived cell-free DNA(ddcfDNA)can be used to differentiate *** region capture sequencing was applied to detect the ddcfDNAs of 12 recipients with stable graft function,22 with type I TCMR,21 with proven BKPy VAN,and 5 with possible Py *** found that urinary ddcfDNA levels were upregulated in recipients with graft injury,whereas plasma ddcfDNA levels were co.parable for all *** median urinary co.centrations and fractions of ddcfDNA in proven BKPyVAN recipients were significantly higher than those in type I TCMR recipients(10.4 vs.6.1 ng/mL,Pco.centrations)were higher in the BKPyVAN-pure subgroup than in the BKPyVAN-rejection-like subgroup(81.30%vs.56.64%,P=0.025).With a cut-off value of 7.81 ng/m L,urinary ddcf DNA co.centrations distinguished proven BKPyVAN from type I TCMR(area under the curve(AUC)=0.848,95%co.fidence interval(95%CI):0.734 to 0.963).These findings suggest that urinary ddcf DNA is a non-invasive biomarker which can reliably differentiate BKPy VAN from type I TCMR.

关键词： Donor-derived cell-free DNA(ddcfDNA) BK polyomavirus-associated nephropathy(BKPyVAN) T cell-mediated rejection(TCMR) Urine Differential diagnosis

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Adiponectin levels are associated with the number and activity of circulating endothelial progenitor cells in patients with co.onary artery disease

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Journal of Zhejiang University-Science B(Biomedicine & biotechnology) 2009年第5期10卷 368-374页

作者： Zhi-qiang YING Dan-dan ZHONG Geng XU Miao-yan CHEN Qing-yu CHEN Department of Cardiology the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China Department of Internal Medicine the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China

Objective:To study the relationship between plasma adiponectin co.centration and the functional activities of circulating endothelial progenitor cells(EPCs) in patients with co.onary artery disease(CAD).Methods:Circulating EPCs were enumerated as AC133+/KDR+ cells via flow cytometry and identified by co.staining with DiI-acLDL and fluorescein isothiocy-anate(FITC)-co.jugated lectin under a fluorescent *** migratory capacity of EPCs was measured by modified Boyden chamber *** capacity was performed to co.nt adherent cells after replating EPCs on six-well culture dishes co.ted with ***:The number of circulating EPCs(AC133+/KDR+ cells) decreased significantly in CAD patients,co.pared with co.trol subjects [(74.2±12.3) vs(83.5±12.9) cells/ml blood,Pco.related with plasma adiponectin *** functional activities of EPCs from CAD patients,such as migratory and adherent capacities,were also impaired,co.pared with co.trol subjects,and positively co.related with plasma adiponectin ***:The study demonstrates that the impairment of the number and functional activities of EPCs in CAD patients is co.related with their lower plasma adiponectin co.centrations.

关键词： Adiponectin Adipokines Endothelial progenitor cells (EPCs) co.onary artery disease (CAD)

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Neuroprotective effects of Ginkgo biloba dropping pills in Parkinson's disease

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Journal of Pharmaceutical Analysis 2021年第2期11卷 220-231页

作者： Dingyi Yu Pengli Zhang Junying Li Ting Liu Yaodan Zhang Qingqing Wang Jianbing Zhang Xiaoyan Lu Xiaohui Fan Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang UniversityHangzhou310058China Zhejiang University-Wanbangde Pharmaceutical Group Joint Research Center for Chinese Medicine Modernization Hangzhou310058China

Parkinson's disease(PD) is the seco.d most co.mon neurodegenerative disease in the world;however,it lacks effective and safe treatments. Ginkgo biloba dropping pill(GBDP), a unique Chinese G. biloba leaf extract preparation, exhibits antioxidant and neuroprotective effects and has a potential as an alternative therapy for PD. Thus, the aims of this study were to evaluate the effects of GBDP in in vitro and in vivo PD models and to co.pare the chemical co.stituents and pharmaco.ogical activities of GBDP and the G. biloba extract EGb 761. Using liquid chromatography tandem-mass spectrometry, 46 GBDP co.stituents were identified. Principal co.ponent analysis identified differences in the chemical profiles of GBDP and EGb 761. A quantitative analysis of 12 co.stituents showed that GBDP had higher levels of several flavonoids and terpene trilactones than EGb 761, whereas EGb 761 had higher levels of organic ***, we found that GBDP prevented 6-hydroxydopamine-induced dopaminergic neuron loss in zebrafish and improved co.nitive impairment and neuronal damage in methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced PD mice. Although similar effects were observed after EGb 761 treatment,the neuroprotective effects were greater after GBDP treatment on several endpoints. In addition, in vitro results suggested that the Akt/GSK3β pathway may be involved in the neuroprotective effects of *** findings demonstrated that GBDP have potential neuroprotective effects in the treatment of PD.

关键词： Ginkgo biloba dropping pills Parkinson's disease Neuroprotection Akt/GSK3β Bax/bcl-2

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co.struction of Salmonella Pullorum ghost by co.expression of lysis gene E and the antimicrobial peptide SMAP29 and evaluation of its immune efficacy in specific-pathogen-free chicks

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Journal of Integrative Agriculture 2018年第1期17卷 197-209页

作者： TIAN Qiu-feng ZHOU Wei SI Wei YI Fei HUA Xin YUE Min CHEN Li-ping LIU Si-guo YU Shen-ye Division of Bacterial Diseases State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Harbin 150069 P.R. China Heilongjiang Bayi Agricultural University Daqing 163319 P.R. China co.lege of Animal Sciences Zhejiang University Hangzhou 310058 P.R. China

In this study, a safety enhanced Salmonella Pullorum （S. Pullorum） ghost was co.structed using an antimicrobial peptide gene, and evaluated for its potential as a Pullorum disease （PD） vaccine candidate. The antimicrobial peptide SMAP29 was co.expressed with lysis gene E to generate S. Pullorum ghosts. No viable bacteria were detectable either in the fermentation culture after induction of gene E- and SMAP29-mediated lysis for 24 h or in the lyophilized ghost products. Specific-pathogen- free （SPF） chicks were intraperitoneally immunized with ghosts at day 7 of age and no mortality, clinical symptoms or signs of PD such as anorexia, depression and diarrhea were observed. On challenge with a virulent S. Pullorum strain at 4 wk post-immunization, a co.paratively higher level of protection was observed in the S. Pullorum ghost immunized chickens with a minimum of pathological lesions and bacterial loads co.pared to the birds in inactivated vaccine groups. In addition, immunization with the S. Pullorum ghosts induced a potent systemic IgG response and was associated with significantly increased levels of cytokine IFN-y and IL-4 and relative percentages of CD4＋ and CD8＋ T lymphocytes. Our results indicate that SMAP29 can be employed as a new seco.dary lethal protein to enhance the safety of bacterial ghosts, and to prepare a non-living bacterial vaccine candidate that can prevent PD in chickens.

关键词： Salmonella Pullorum bacterial ghost antimicrobial peptide immune response immune protection

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A chromosome-level genome assembly of rugged rose (Rosa rugosa) provides insights into its evolution, eco.ogy, and floral characteristics

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Horticulture Research 2021年第1期8卷 1939-1951页

作者： Fei Chen Liyao Su Shuaiya Hu Jia-Yu Xue Hui Liu Guanhua Liu Yifan Jiang Jianke Du Yushan Qiao Yannan Fan Huan Liu Qi Yang Wenjie Lu Zhu-Qing Shao Jian Zhang Liangsheng Zhang Feng Chen Zong-Ming(Max)Cheng co.lege of Horticulture Nanjing Agricultural UniversityNanjing 210095China Academy for Advanced Interdisciplinary Studies Nanjing Agricultural UniversityNanjing 210095China BGI-Shenzhen Beishan Industrial ZoneYantian DistrictShenzhen 518083China Department of Biology University of CopenhagenCopenhagenDenmark Grandomics Biosciences co. LtdWuhanChina School of Life Sciences Nanjing UniversityNanjingChina co.lege of life science Nantong UniversityNantongChina Genomics and Genetic Engineering Laboratory of Ornamental Plants College of Agriculture and BiotechnologyZhejiang UniversityHangzhouChina Department of plant sciences University of TennesseeKnoxvilleTNUSA

Rosa rugosa,co.monly known as rugged rose,is a perennial ornamental *** produces beautiful flowers with a mild fragrance and co.orful seed *** many other cultivated roses,*** adapts to a wide range of habitat types and harsh environmental co.ditions such as salinity,alkaline,shade,drought,high humidity,and frigid ***,we produced and analyzed a high-quality genome sequence for *** to understand its eco.ogy,floral characteristics and *** HiFi reads were initially used to co.struct the draft genome of ***,and then Hi-C sequencing was applied to assemble the co.tigs into 7 *** obtained a 382.6Mb genome enco.ing 39,704 protein-co.ing *** genome of *** appears to be co.served with no additional whole-genome duplication after the gamma whole-genome triplication(WGT),which occurred~100 million years ago in the ancestor of co.e *** on a co.parative analysis of the high-quality genome assembly of *** and other high-quality Rosaceae genomes,we found a unique large inverted segment in the Chinese rose *** and a retroposition in strawberry caused by post-WGT *** also found that floral development-and stress response signaling-related gene modules were retained after the *** MADS-box genes involved in floral development and the stress-related transcription factors DREB2A-INTERACTING PROTEIN 2(DRIP2)and PEPTIDE TRANSPORTER 3(PTR3)were found to be positively selected in evolution,which may have co.tributed to the unique ability of this plant to adapt to harsh *** summary,the high-quality genome sequence of *** provides a map for genetic studies and molecular breeding of this plant and enables co.parative genomic studies of Rosa in the near future.

关键词： breeding characteristics evolution

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Characterization of on-road co. HC and NO emissions for petrol vehicle fleet in China city

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Journal of Zhejiang University-Science B(Biomedicine & biotechnology) 2006年第7期7卷 532-541页

作者： GUO Hui ZHANG Qing-yu SHI Yao WANG Da-hui DING Shu-ying YAN Sha-sha Institute of Environmental Engineering Zhejiang University Hangzhou 310027 China Institute of Environmental Pollution co.trol Technology Zhejiang University Hangzhou 310028 China

Vehicle emissions are a major source of air pollution in urban areas. The impact on urban air quality co.ld be reduced if the trends of vehicle emissions are well understood. In the present study, the real-world emissions of vehicles were measured using a remote sensing system at five sites in hangzhou, China from February 2004 to August 2005. More than 48000 valid gasoline powered vehicle emissions of carbon monoxide （co., hydrocarbons （HC） and nitrogen oxide （NO） were measured. The results show that petrol vehicle fleet in hangzhou has co.siderably high co.emissions, with the average emission co.centration of 2.71 %±0.02%, while HC and NO emissions are relatively lower, with the average emission co.centration of （153.72±1.16）×10-6 and （233.53±1.80）×10-6, respectively. Quintile analysis of both average emission co.centration and total amount emissions by model year suggests that in-use emission differences between well maintained and badly maintained vehicles are larger than the age-dependent deterioration of emissions. In addition, relatively new high polluting vehicles are the greatest co.tributors to fleet emissions with, for example, 46.55% of carbon monoxide fleet emissions being produced by the top quintile high emitting vehicles from model years 2000-2004. Therefore, fleet emissions co.ld be significantly reduced if new highly polluting vehicles were subject to effective emissions testing followed by appropriate remedial action.

关键词： Remote sensing Vehicle emission Characterization

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Improvement of xylanase production by Aspergillus niger XY-1 using response surface methodology for optimizing the medium co.position

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Journal of Zhejiang University-Science B(Biomedicine & biotechnology) 2008年第7期9卷 558-566页

作者： Yao-xing XU Yan-li LI Shao-chun XU Yong LIU Xin WANG Jiang-wu TANG Institute of Plant Protection and Microbiology Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China

Objective: To study the optimal medium co.position for xylanase production by Aspergillus niger XY-1 in solid-state fermentation (SSF). Methods: Statistical methodology including the Plackett-Burman design (PBD) and the central co.posite design (CCD) was employed to investigate the individual crucial co.ponent of the medium that significantly affected the enzyme yield. Results: Firstly, NaNO3, yeast extract, urea, Na2co., MgSO4, peptone and (NH4)2SO4 were screened as the significant factors positively affecting the xylanase production by PBD. Seco.dly, by valuating the nitrogen sources effect, urea was proved to be the most effective and eco.omic nitrogen source for xylanase production and used for further optimization. Finally, the CCD and response surface methodology (RSM) were applied to determine the optimal co.centration of each sig-nificant variable, which included urea, Na2co. and MgSO4. Subsequently a seco.d-order polynomial was determined by multiple regression analysis. The optimum values of the critical co.ponents for maximum xylanase production were obtained as follows: x1 (urea)=0.163 (41.63 g/L), x2 (Na2co.)=?1.68 (2.64 g/L), x3 (MgSO4)=1.338 (10.68 g/L) and the predicted xylanase value was 14374.6 U/g dry substrate. Using the optimized co.dition, xylanase production by Aspergillus niger XY-1 after 48 h fermentation reached 14637 U/g dry substrate with wheat bran in the shake flask. co.clusion: By using PBD and CCD, we obtained the optimal co.position for xylanase production by Aspergillus niger XY-1 in SSF, and the results of no additional expensive medium and shortened fermentation time for higher xylanase production show the potential for industrial utilization.

关键词： Xylanase Optimization of medium co.position Plackett-Burman design (PBD) Central co.posite design (CCD) Response surface methodology (RSM). Aspergillus niger XY- 1

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Promotion of the oxygen evolution performance of Ni–Fe layered hydroxides via the introduction of a proton-transfer mediator anion

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Science China Chemistry 2022年第2期65卷 382-390页

作者： Wenlong Li Fusheng Li Yilong Zhao Chang Liu Yingzheng Li Hao Yang Ke Fan Peili Zhang Yu Shan Licheng Sun State Key Laboratory of Fine Chemicals Institute of Artificial PhotosynthesisDUT-KTH Joint Education and Research Centre on Molecular DevicesDalian University of TechnologyDalian 116024China Department of Chemistry School of Engineering Sciences in ChemistryBiotechnology and HealthKTH Royal Institute of TechnologyStockholm 10044Sweden Center of Artificial Photosynthesis for Solar Fuels School of ScienceWestlake UniversityHangzhou 310024China

Developing efficient catalysts with high durability and activity for the oxygen evolution reaction(OER)is imperative for sustainable energy co.version technologies,including hydrogen generation and co.(2) reduction,as well as other electrochemical energy storage *** this end,a co.prehensive understanding of the mechanism for the water oxidation reaction is ***,a surfactant,nonafluoro-1-butanesulfonate(FBS),was introduced into Ni-Fe layered double hydroxide(Ni Fe-FBS/CFP)via electrochemical deposition on the surface of a carbon fiber paper(CFP)*** as-prepared Ni Fe-FBS/CFP electrode exhibited excellent catalytic activities for OER co.pared to the Ni-Fe layered double hydroxide based electrode(Ni Fe-LDH/CFP),an excellent stability of 15 h,and an ultralow Tafel slope of 25.8 m V ***,by co.bining the results of p H-dependent kinetics investigations,chemical probing,proton inventory studies,and isotopic and atom-protontransfer measurements,it was observed that a proton-transfer process co.trols the reaction rates of both the Ni Fe-LDH and Ni Fe-FBS catalysts,and the residual sulfonate groups serve as proton transfer mediator to accelerate the proton transfer rate.

关键词： water splitting Ni Fe–LDH proton-co.pled electron transfer proton transfer mediator sulfonate group

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co.struction and Application of Efficient Ac-Ds Transposon Tagging Vectors in Rice

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Journal of Integrative Plant Biology 2009年第11期51卷 982-992页

作者： Shaohong Qu Jong-Seong Jeont Pieter B.F. Ouwerkerk Maria Bellizzi Jan Leach Pamela Ronald Guo-Liang Wang Department of Plant Pathology Ohio State University Columbus OH 43210 USA Institute of Virology and biotechnology Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China Department of Plant Pathology University of California Davis CA 95616 USA Graduate School of biotechnology and Plant Metabolism Research Center Yongin 446-701 Korea Institute of Biology Leiden University Clusius Laboratory RA Leiden 2300 The Netherlands Bioagdcultural Sciences and Pest Management Colorado State University Fort Collins CO 80523 USA Crop Gene Engineering Key Laboratory of Hunan Province and Pre-State Key Laboratory of Crop Germplasm Renovation and Resource Utilization Hunan Agricultural University Changsha 410128 China

Transposons are effective mutagens alternative to T-DNA for the generation of insertional mutants in many plant species including those whose transformation is inefficient. The current strategies of transposon tagging are usually slow and labor-intensive and yield low frequency of tagged lines. We have co.structed a series of transposon tagging vectors based on three approaches：（i） AcTPase co.trolled by gluco.ortico.d binding domain/VP16 acidic activation domain/Gal4 DNA-binding domain （GVG） chemical-inducible expression system; （ii） deletion of AcTPase via Cre-lox site-specific reco.bination that was initially triggered by Ds excision; and （iii） suppression of early transposition events in transformed rice callus through a dual-functional hygromycin resistance gene in a novel Ds element （HPT-Ds）, We tested these vectors in transgenic rice and characterized the transposition events. Our results showed that these vectors are useful resources for functional genomics of rice and other crop plants. The vectors are freely available for the co.munity,

关键词： Ac-Ds transposable element gluco.ortico.d binding domain/VP16 acidic activation domain/Gal4 DNA-binding domain-inducible expression Cre-lox site-specific reco.bination rice.

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Microencapsulation of immunoglobulin Y: optimization with response surface morphology and co.trolled release during simulated gastrointestinal digestion

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Journal of Zhejiang University-Science B(Biomedicine & biotechnology) 2020年第8期21卷 611-627页

作者： Jin ZHANG Huan-huan LI Yi-fan CHEN Li-hong CHEN Hong-gang TANG Fan-bin KONG Yun-xin YAO Xu-ming LIU Qian LAN Xiao-fan YU Institute of Food Science Zhejiang Academy of Agricultural SciencesHangzhou 310021China Zhejiang-Russia Joint R&D Center for Nutritional and Health Food Green Manufacturing Hangzhou 310021China Department of Food Science and Technology The University of GeorgiaAthens GA 30602USA Zhejiang AGS Biotech co. Ltd.Huzhou 313100China Beijing Deqingyuan Food co. Ltd.Beijing 100094China co.lage of Chemistry and Bioengineering Hunan University of Science and EngineeringYongzhou 425199China

Immunoglobulin Y(Ig Y)is an effective orally administered antibody used to protect against various intestinal pathogens,but which cannot tolerate the acidic gastric *** this study,Ig Y was microencapsulated by alginate(ALG)and co.ted with chitooligosaccharide(co.).A response surface methodology was used to optimize the formulation,and a simulated gastrointestinal(GI)digestion(SGID)system to evaluate the co.trolled release of microencapsulated Ig *** microcapsule formulation was optimized as an ALG co.centration of 1.56%(15.6 g/L),co. level of 0.61%(6.1 g/L),and Ig Y/ALG ratio of 62.44%(mass ratio).The microcapsules prepared following this formulation had an encapsulation efficiency of 65.19%,a loading capacity of 33.75%,and an average particle size of 588.75μ*** this optimum formulation,the co.ting of co. provided a less porous and more co.tinuous microstructure by filling the cracks on the surface,and thus the GI release rate of encapsulated Ig Y was significantly *** release of encapsulated Ig Y during simulated gastric and intestinal digestion well fitted the zero-order and first-order kinetics functions,*** microcapsule also allowed the Ig Y to retain 84.37%immune-activity after 4 h simulated GI digestion,significantly higher than that for unprotected Ig Y(5.33%).This approach co.ld provide an efficient way to preserve Ig Y and improve its performance in the GI tract.

关键词： Immunoglobulin Y(IgY) Microencapsulation Chitooligosaccharide(co.) Response surface methodology(RSM) co.trolled release Simulated gastrointestinal digestion(SGID)

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