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Identification and characterization of MeERF genes and their targets in pathogen response by cassava(Manihot esculenta)

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The Crop Journal 2021年第5期9卷 1145-1153页

作者： Yuhui Hong Yong Xiao Na Song Shousong Zhu Rui Zhao Ke Li Mengting Geng Xiaohui Yu Honggang Wang Wei Xia Yinhua Chen hainan key laboratory for sustainable utilization of tropical bioresource College of Tropical CropsHainan UniversityHaikou 570228HainanChina Coconut Research Institute Chinese Academy of Tropical Agricultural SciencesWenchang 571339HainanChina School of Bioengineering Dalian University of TechnologyDalian 116024LiaoningChina

Cassava,Manihot esculenta Crantz (Me),is a major dietary source of calories for over 700 million people in tropical *** production of cassava is constantly threatened by cassava bacterial blight (CBB),caused by Xanthomonas axonopodis *** (Xam).The gene resources for CBB-resistant breeding of cassava are *** model plant species,ethylene response factors play important roles in response to pathogen *** this study,cassava ethylene response factors (MeERFs) were identified and characterized as the first step in studying their potential for CBB-resistant breeding of *** the cassava genome 155 MeERFs were identified,of which 23 were induced by Xam *** promoter regions of204 genes harbored GCC-box that had the potential to interact with *** 37 transcriptomes derived from Xam infection treatment,four gene co-expression modules for the MeERFs and GCC-box containing genes were *** MeERFs were associated with two GCC-box containing genes:transcription initiation factor TFIIE subunit beta (MeTFIIE),and histone-lysine N-methyltransferase ASHR1 (MeASHR1).Dual-luciferase reporter assays showed that MeERF10 and MeERF58 positively regulated Me TFIIE;MeERF137 negatively regulated Me TFIIE;MeERF10 and MeERF137 positively regulated Me ASHR1;and MeERF35 negatively regulated Me *** four MeERFs may mediate pathogen response by regulating the expression of the two GCC-box containing genes.

关键词： Cassava Ethylene response factor Expression profile Co-expression analysis Pathogen

Arabidopsis PED_2 positively modulates plant drought stress resistance

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Journal of Integrative Plant Biology 2015年第9期57卷 796-806页

作者： Haitao Shi Tiantian Ye Fan Yang Zhulong Chan hainan key laboratory for sustainable utilization of tropical bioresources College of Agriculture Hainan University key laboratory of Plant Germplasm Enhancement and Specialty Agriculture Wuhan Botanical Garden Chinese Academy of Sciences University of Chinese Academy of Sciences

Abscisic acid (ABA) is an important phytohormone that functions in seed germination, plant development, and multiple stress responses. Arabidopsis Peroxisome defective 2 (AtPED2) (also known as AtPEXOXIN14, AtPEX14), is involved in the intracellular transport of thiolase from the cytosol to glyoxysomes, and perosisomal matrix protein import in plants. In this study, we assigned a new role for AtPED2 in drought stress resistance. The transcript level of AtPED2 was downregulated by ABA and abiotic stress treatments. AtPED2 knockout mutants were insensitive to ABA-mediated seed germination, primary root elongation, and stomatal response, while AtPED2 over-expressing plants were sensitive to ABA in comparison to wide type (WT). AtPED2 also positively regulated drought stress resistance, as evidenced by the changes of water loss rate, electrolyte leakage, and survival rate. Notably, AtPED2 positively modulated expression of several stress-responsive genes (RAB18, RD22, RD29A, and RD29B), positively affected underlying antioxidant enzyme activities and negatively regulated reactive oxygen species (ROS) level under drought stress conditions. Moreover, multiple carbon metabolites including amino acids, organic acids, sugars, sugar alcohols, and aromatic amines were also positively regulated by AtPED2. Taken together, these results indicated a positive role for AtPED2 in drought resistance, through modulation of stress-responsive genes expression, ROS metabolism, and metabolic homeostasis, at least partially.

关键词： Abscisic acid Arabidopsis drought stress metabolite oxygen species PED2 reactive

MaDREB1F confers cold and drought stress resistance through common regulation of hormone synthesis and protectant metabolite contents in banana

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Horticulture Research 2023年第2期10卷 227-240页

作者： Yi Xu Wei Hu Shun Song Xiaoxue Ye Zehong Ding Juhua Liu Zhuo Wang Jingyang Li Xiaowan Hou Biyu Xu Zhiqiang Jin Haikou Experimental Station Key Laboratory of Genetic Improvement of BananasSanya Research InstituteState Key Laboratory of Biological Breeding for Tropical CropsInstitute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHainanChina hainan key laboratory for Protection and utilization of tropical bioresources Hainan Institute for Tropical Agricultural ResourcesChinese Academy of Tropical Agricultural SciencesHainanChina hainan Yazhou Bay Seed laboratory HainanChina hainan key laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions Key Laboratory of Biology and Genetic Resources of Tropical CropsInstitute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHainanChina key laboratory of hainan Province for Postharvest Physiology and Technology of tropical Horticultural Products South Subtropical Crops Research InstituteChinese Academy of Tropical Agricultural SciencesGuangdongChina

Adverse environmental factors severely affect crop *** crop resistance to multiple stressors is an important breeding *** CBFs/DREB1s extensively participate in plant resistance to abiotic stress,the common mechanism underlying CBFs/DREB1s that mediate resistance to multiple stressors remains ***,we show the common mechanism for MaDREB1F conferring cold and drought stress resistance in ***1F encodes a dehydration-responsive element binding protein(DREB)transcription factor with nuclear localization and transcriptional ***1F expression is significantly induced after cold,osmotic,and salt ***1F overexpression increases banana resistance to cold and drought stress by common modulation of the protectant metabolite levels of soluble sugar and proline,activating the antioxidant system,and promoting jasmonate and ethylene *** analysis shows that MaDREB1F activates or alleviates the repression of jasmonate and ethylene biosynthetic genes under cold and drought ***,MaDREB1F directly activates the promoter activities of MaAOC4 and MaACO_(2)0 for jasmonate and ethylene syntheses,respectively,under cold and drought ***1F also targets the MaERF11 promoter to activate MaACO_(2)0 expression for ethylene synthesis under drought ***,our findings offer new insight into the common mechanism underlying CBF/DREB1-mediated cold and drought stress resistance,which has substantial implications for engineering cold-and drought-tolerant crops.

关键词： synthesis sugar finding

Effects of Y2O3 on the microstructure and mechanical properties ofspark plasma sintered fine-grained W-Ni-Mn alloy

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Journal of Rare Earths 2017年第11期35卷 1149-1155页

作者： Yanlin Pan Lei Ding Hui Li Daoping Xiang State key laboratory of Marine Resource utilization in South China Sea Hainan University Haikou 570228 China key laboratory for Advanced Materials in tropical Island Resources Ministry of Education Hainan University Haikou 570228 China

Fine-grained W-Ni-Mn-YOalloys were fabricated by mechanical alloying-assisted spark plasma sintering(SPS), and the effects of YOcontent on the microstructure and mechanical properties of the alloys were studied. Fine-grained 90 W-6 Ni-4 Mn-YOalloys with uniform microstructure and excellent properties were prepared by SPS at 1150 ℃. The addition of trace YOinhibited the sintering densification process and refined the W grain size. The average W grain size decreased from 5.5 μm to 2.1 μ*** fracture mode changed from W grain transgranular fracture and W-W interface fracture to W-W and W-Matrix phase interface fracture. The Rockwell hardness and bending strength of alloys initially increased and then decreased with increasing YOcontent. The optimum comprehensive mechanical properties(Rockwell hardness and bending strength) of the alloys were obtained at the same time when the mass fraction of YOwas 0.4%.

关键词： 90W-6Ni-4Mn alloys Y_2O_3 Spark plasma sintering Bending strength Fracture morphology

Adsorption–desorption behavior of atrazine on agricultural soils in China

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Journal of Environmental Sciences 2017年第7期29卷 180-189页

作者： Lin Yue ChengJun Ge Dan Feng Huamei Yu Hui Deng Bomin Fu School of Environment and Plant Protection Hainan UniversityHaikou 570228China key laboratory of Protection and Development utilization of tropical Crop Germplasm Resources(hainan University) Ministry of EducationHaikou 570228HainanChina

Adsorption and desorption are important processes that affect atrazine transport,transformation,and bioavailability in *** this study,the adsorption–desorption characteristics of atrazine in three soils（laterite,paddy soil and alluvial soil） were evaluated using the batch equilibrium *** results showed that the kinetics of atrazine in soils was completed in two steps：a＂fast＂ adsorption and a ＂slow＂ adsorption and could be well described by pseudo-second-order *** addition,the adsorption equilibrium isotherms were nonlinear and were well fitted by Freundlich and Langmuir *** was found that the adsorption data on laterite,and paddy soil were better fitted by the Freundlich model;as for alluvial soil,the Langmuir model described it *** maximum atrazine sorption capacities ranked as follows：paddy soil 〉 alluvial soil 〉 *** of thermodynamic calculations indicated that atrazine adsorption on three tested soils was spontaneous and *** desorption data showed that negative hysteresis ***,lower solution pH value was conducive to the adsorption of atrazine in *** atrazine adsorption in these three tested soils was controlled by physical adsorption,including partition and surface *** lower equilibrium concentration,the atrazine adsorption process in soils was dominated by surface adsorption;while with the increase of equilibrium concentration,partition was predominant.

关键词： Atrazine Adsorption Desorption Agricultural soils

The Structure and Function of Major Plant Metabolite Modifications

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Molecular Plant 2019年第7期12卷 899-919页

作者： Shouchuang Wang Saleh Alseekh Alisdair R.Fernie Jie Luo hainan key laboratory for sustainable utilization of tropical bioresource College of Tropical Crops Hainan University Haikou 572208 China Max Planck Institute of Molecular Plant Physiology Potsdam-Golm 14476 Germany Centre of Plant Systems Biology and Biotechnology Plovdiv 4000 Bulgaria National key laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Huazhong Agricultural University Wuhan 430070 China

Plants produce a myriad of structurally and functionally diverse metabolites that play many different roles in plant growth and development and in plant response to continually changing environmental conditions as well as abiotic and biotic stresses. This metabolic diversity is, to a large extent, due to chemical modification of the basic skeletons of metabolites. Here, we review the major known plant metabolite modifications and summarize the progress that has been achieved and the challenges we are facing in the field. We focus on discussing both technical and functional aspects in studying the influences that various modifications have on biosynthesis, degradation, transport, and storage of metabolites, as well as their bioactivity and toxicity. Finally, we discuss some emerging insights into the evolution of metabolic pathways and metabolite functionality.

关键词： metabolic diversity metabolite modification genetic basis evolution of metabolism

Development of organelle single nucleotide polymorphism (SNP) markers and their application for the identification of cytoplasmic inheritance patterns in Pyropia yezoensis (Bangiales,Rhodophyta)

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Journal of Oceanology and Limnology 2021年第4期39卷 1447-1457页

作者： Lu WANG Junhao WANG Yunke ZHU Zhengcai CUI Fanna KONG Xianghai TANG Yunxiang MAO key laboratory of Marine Genetics and Breeding(Ministry of Education) College of Marine Life SciencesOcean University of ChinaQingdao 266003China key laboratory of utilization and Conservation of tropical Marine bioresource(Ministry of Education) College of Fisheries and Life ScienceHainan Tropical Ocean UniversitySanya 572022China

The genus Pyropia contains several important cultivated *** research in nori species has mainly focused on the cell nucleus,with few studies on organelles(chloroplast and mitochondria).Due to the high copy numbers of organelles in cells,which influence the development and traits of algae,it is necessary to study their genetic *** this study,the marine red alga Pyropia yezoensis,an important economic macroalga,was selected as the study *** investigate organelle(chloroplast and mitochondria)inheritance in ***,the wild type RZ(maternal strain)was crossed with the red mutant HT(paternal strain)and 30 color-sectors from 11 F1 gametophytic blades were *** complete chloroplast and mitochondrial genomes of the red mutant(HT)were assembled for the first *** reliable and stable single nucleotide polymorphism(SNP)loci filtrated by bioinformatics analysis was used as a molecular marker for chloroplast and mitochondrial DNA,respectively,in subsequent *** amplification and sequence analysis showed that the haplotypes of color-sectors detected were consistent with those of the maternal parent,confirming that both chloroplast and mitochondrial genomes were inherited maternally in *** inheritance pattern of organelles in *** can be used to guide the hybridization and breeding of ***,the organelle SNP markers developed in this study can be applied in subsequent genetic research.

关键词： Pyropia yezoensis organelle single nucleotide polymorphism(SNP)markers chloroplast mitochondrial organelle inheritance maternal inheritance

On microbial community of Pyropia haitanensis by metagenomic analysis

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Journal of Oceanology and Limnology 2021年第3期39卷 1091-1102页

作者： Junhao WANG Yunxiang MAO Guoying DU Xiaojiao LI Xianghai TANG key laboratory of Marine Genetics and Breeding(Ministry of Education) College of Marine Life SciencesOcean University of ChinaQingdao 266003China key laboratory of utilization and Conservation of tropical Marine bioresource(Ministry of Education) College of Fisheries and Life ScienceHainan Tropical Ocean UniversitySanya 572022China

Microorganisms plays an important role in the growth of Pyropia *** understand the structural and functional diversity of the microorganism community of ***(PH40),the associated metabolic pathway network in cluster of orthologous groups(COG)and Kyoto Encyclopedia of Genes and Genomes(KEGG),and carbohydrate-active enzymes(CAZymes)were explored in metagenomic *** extraction from gametophytes of *** was performed first,followed by library construction,sequencing,preprocessing of sequencing data,taxonomy assignment,gene prediction,and functional *** results show that the predominant microorganisms of *** were bacteria(98.98%),and the phylum with the highest abundance was Proteobacteria(54.64%),followed by Bacteroidetes(37.92%).Erythrobacter(3.98%)and Hyunsoonleella jejuensis(1.56%)were the genera and species with the highest abundance of bacteria,*** COG annotation demonstrated that genes associated with microbial metabolism was the predominant *** results of metabolic pathway annotation show that the ABC transport system and two-component system were the main pathways in the microbial *** growth hormone biosynthesis pathway and multi-vitamin biosynthesis functional units(modules)were the other important *** CAZyme annotation revealed that the starch might be an important carbon source for *** transferase family 2(GT2)and glycosyl transferase family 3(GT3)were the highly abundant families in glucoside transferase *** metagenome-assembled genomes containing enzymes involved in the biosynthesis of cobalamin(vitamin B 12)and indole-3-acetic acid were obtained by binning *** were confirmed to belong to Rhodobacterales and Rhizobiales,*** findings provide comprehensive insights into the microorganism community of Pyropia.

关键词： P.haitanensis metagenomic microbial community cluster of orthologous groups(COG) Kyoto Encyclopedia of Genes and Genomes(KEGG)pathways carbohydrate-active enzymes(CAZymes)

Quantification of Photosynthetic Pigments in Neopyropia yezoensis Using Hyperspectral Imagery

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Plant Phenomics 2023年第1期5卷 60-72页

作者： Shuai Che Guoying Du Xuefeng Zhong Zhaolan Mo Zhendong Wang Yunxiang Mao key laboratory of Marine Genetics and Breeding(Ministry of Education) College of Marine Life SciencesOcean University of ChinaQingdao266003China key laboratory of utilization and Conservation of tropical Marine bioresource(Ministry of Education) College of Fisheries and Life ScienceHainan Tropical Ocean UniversitySanya572002China Yazhou Bay Innovation Institute Hainan Tropical Ocean UniversitySanya572025China laboratory for Marine Biology and Biotechnology Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao266073China

Phycobilisomes and chlorophyll-a(Chla)play important roles in the photosynthetic physiology of red macroalgae and serve as the primary light-harvesting antennae and reaction center for photosystem Ⅱ.Neopyropia is an economically important red macroalga widely cultivated in East Asian *** contents and ratios of 3 main phycobiliproteins and Chla are visible traits to evaluate its commercial *** traditional analytical methods used for measuring these components have several ***,a high-throughput,nondestructive,optical method based on hyperspectral imaging technology was developed for phenotyping the pigments phycoerythrin(PE),phycocyanin(PC),allophycocyanin(APC),and Chla in Neopyropia thalli in this *** average spectra from the region of interest were collected at wavelengths ranging from 400 to 1000 nm using a hyperspectral *** different preprocessing methods,2 machine learning methods,partial least squares regression(PLSR)and support vector machine regression(SVR),were performed to establish the best prediction models for PE,PC,APC,and Chla *** prediction results showed that the PLSR model performed the best for PE(R_(Test^(2))=0.96,MAPE=8.31%,RPD=5.21)and the SVR model performed the best for PC(R_(Test^(2))=0.94,MAPE=7.18%,RPD=4.16)and APC(R_(Test^(2))=0.84,MAPE=18.25%,RPD=2.53).Two models(PLSR and SVR)performed almost the same for Chla(PLSR:R_(Test^(2))=0.92,MAPE=12.77%,RPD=3.61;SVR:R_(Test^(2))=0.93,MAPE=13.51%,RPD=3.60).Further validation of the optimal models was performed using field-collected samples,and the result demonstrated satisfactory robustness and *** distribution of PE,PC,APC,and Chla contents within a thallus was visualized according to the optimal prediction *** results showed that hyperspectral imaging technology was effective for fast,accurate,and noninvasive phenotyping of the PE,PC,APC,and Chla contents of Neopyropia in *** could benefit the efficiency of macro

关键词： prediction breeding visible