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Evidence for the involvement of NOD2 in regulating colonic epithelial cell growth and survival

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World Journal of Gastroenterology 2008年第38期14卷 5834-5841页

作者： Sheena M Cruickshank Louise Wakenshaw John Cardone Peter D Howdle Peter J Murray Simon R Carding Molecular and Cellular Biology University of Leeds Leeds LS2 9JT United Kingdom Faculty of Life Sciences University of Manchester Manchester M13 9PT United Kingdom Institute of Molecular and Cellular Biology University of Leeds Leeds LS2 9JT United Kingdom The Institute of Food Research Norwich Research Park Norwich NR4 7UA United Kingdom Institute of Molecular and Cellular Biology University of Leeds Leeds LS2 9JT United Kingdom Section of Medicine Surgery & Anaesthesia Leeds Institute of Molecular Medicine University of Leeds Leeds LS2 9JT United Kingdom Department of Infectious Diseases St. Jude Children's Research Hospital Memphis TN 38105 United States

AIM: To investigate the function of NOD2 in colonic epithelial cells (CEC). METHODS: A combination of in vivo and in vitro analyses of epithelial cell turnover in the presence and absence of a functional NOD2 protein and, in response to enteric Salmonella typhimurium infection, were used. shRNA interference was also used to investigate the consequences of knocking down NOD2 gene expression on the growth and survival of colorectal carcinoma cell lines. RESULTS:In the colonic mucosa the highest levels of NOD2 expression were in proliferating crypt epithelial cells. Muramyl dipeptide (MDP), that is recognized by NOD2, promoted CEC growth in vitro . By contrast,the growth of NOD2-deficient CECs was impaired. In vivo CEC proliferation was also reduced and apoptosis increased in Nod2-/- mice, which were also evident following enteric Salmonella infection. Furthermore, neutralization of NOD2 mRNA expression in human colonic carcinoma cells by shRNA interference resulted in decreased survival due to increased levels of apoptosis. CONCLUSION: These findings are consistent with the involvement of NOD2 protein in promoting CEC growth and survival. Defects in proliferation by CECs in cases of CD may contribute to the underlying pathology of disrupted intestinal homeostasis and excessive inflammation.

关键词：结肠上皮细胞 NOD2 细胞生长功能蛋白

先天性上睑下垂患儿行自身掌长肌额肌悬吊术的长期疗效

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世界核心医学期刊文摘（眼科学分册） 2005年第9期1卷 32-32页

作者： Wong C.Y. Ng J.S.K. D.S.C. Lam 杨建刚 Department of Ophthalmology and Visual Sciences The Chinese University of Hong Kong Hong Kong Eye Hospital 147 K Argyle Street Kowloon Hong Kong

Purpose: To investigate the long-term outcome of using autogenous palmaris longus tendon (PLT) sling for correcting congenital ptosis in children. Methods: This is an observational case series involving 15 eyelids of 14 consecutive children with congenital ptosis who underwent frontalis suspension surgery using PLT in a university teaching hospital. Results: One child had bilateral ptosis and the other children had unilateral ptosis. The age of patients at the time of surgery ranged from 2 to 7 years, with an average of 4.7 years. At a mean follow-up of 92 months (range, 80-104 months), all eyelids were successfully corrected with good lid height. No recurrence or other postoperative complications were encountered except one patient who developed a small skin fold over the PLT harvest site. Conclusion: Long-term lid position is remarkably stable after surgical correction using PLT. PLT sling appears to be a safe and effective treatment for children with congenital ptosis requiring frontalis sling operation. It could be a good alternative to autogenous fascia lata, and further studies, to compare these two sling materials seem warranted.

关键词：先天性上睑下垂额肌悬吊术掌长肌矫正术大学教学医院阔筋膜长期效果观察性

计算机辅助联合吲哚菁绿分子荧光影像技术在肝脏肿瘤诊断和手术导航中的应用指南（2019版）（英文）

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南方医科大学学报 2019年第10期39卷 1127-1140页

作者：无方驰华 LU Qiping LAU Wanyee 中华医学会数字医学分会中国研究型医院学会数字智能化专业委员会中国医师协会肝癌专业委员会中国医师协会临床精准医学专业委员会中国图学学会医学图像与设备专业委员会中国生物物理学会分子影像学专业委员会南方医科大学珠江医院肝胆一科广东省数字医学临床工程技术研究中心 Department of General Surgery General Hospital of Central Theater Command Faculty of Medicine The Chinese University of Hong Kong

计算机辅助联合吲哚菁绿分子荧光影像技术可以从三维的形态解剖和细胞功能水平进行术前规划和术中侦测,从而导航肝癌解剖性、功能性、根治性的肝切除术,临床应用证明其有重要的诊疗价值,此项技术现已在全国得到了广泛推广和应用。为规... 详细信息

计算机辅助联合吲哚菁绿分子荧光影像技术可以从三维的形态解剖和细胞功能水平进行术前规划和术中侦测,从而导航肝癌解剖性、功能性、根治性的肝切除术,临床应用证明其有重要的诊疗价值,此项技术现已在全国得到了广泛推广和应用。为规范计算机辅助联合吲哚菁绿分子荧光影像技术在肝脏肿瘤精准诊疗中的应用,本指南在以下几个方面进行了研究:(1)三维可视化技术应用流程;(2)ICG分子荧光成像的机制及应用流程;(3)三维可视化和虚拟现实技术的临床应用;(4)ICG分子荧光影像技术的临床应用。ICG分子荧光成像技术能从分子、细胞水平层面实现肿瘤边界界定、肝段和肝叶切除切线的确定;微小病灶或转移灶的侦测;术中根据肝脏肿瘤的荧光信号特点,结合术中快速冰冻病理学检查,可初步判定肝脏占位性病变的分化程度;肝切除后对肝断面进行残留肿瘤病灶和胆漏的检测。计算机辅助联合ICG分子荧光影像技术在肝脏肿瘤诊断和手术导航中的应用,为肝脏肿瘤的外科治疗提供了一种新的数字智能化外科诊疗技术,该项技术亦将不断改进和完善,为肝脏肿瘤的精准诊疗展现出良好的应用前景。

关键词：吲哚菁绿荧光影像三维可视化肝脏肿瘤导航手术

酶促反应动力学教学刍议:米氏方程衍生公式与图像

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化学教育（中英文） 2021年第8期42卷 105-110页

作者：王志鹏车子良马新雨 Farica Zhuang 蒋振雄尹晟王鹏 Department of Chemistry Texas A&M UniversityCollege StationTexas 77840USA Division of Genetics Department of MedicineBrigham and Women’s HospitalDepartment of Biological Chemistry and Molecular PharmacologyHarvard Medical SchoolBostonMA 02115USA Department of Mathematics Harvard UniversityMA 02138USA Citadel Securities LLC:131 South Dearborn St.Chicago Illinois 60603USA Department of Computer Science Duke UniversityDurhamNC 27708USA Department of Biology Texas A&M UniversityTexas 77840USA Duke Center for Genomic and Computational Biology Duke UniversityDurbamNC 27708USA Nutrition/Metabolism Laboratory Department of SurgeryBeth Israel Deaconess Medical CenterHarvard Medical SchoolBostonMA 02138USA 中山大学附属第一医院烧伤外科广州510080

许多国内外高校的本科教学缺乏对米氏方程的深入推衍,这对学生了解酶促反应的动态过程及在实际科研工作中的应用是极为不利的。从酶促反应方程出发对米氏方程进行推导,继之采用双倒数法、单倒数法、直接隐函数法、间接隐函数法以及积分... 详细信息

许多国内外高校的本科教学缺乏对米氏方程的深入推衍,这对学生了解酶促反应的动态过程及在实际科研工作中的应用是极为不利的。从酶促反应方程出发对米氏方程进行推导,继之采用双倒数法、单倒数法、直接隐函数法、间接隐函数法以及积分法对米氏方程进行线性化,得到适用于不同情况的衍生方程。从数学和酶学角度出发指出了不同方程的优势和缺点,并针对其缺陷提出了若干解决方案以及在实际应用过程中的注意事项。希对高校中有志于科研事业的相关专业的教师学生有所助益。

关键词：米氏方程米氏常数酶动力学线性化

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刍议组蛋白去乙酰化酶的结构分类与功能调控

生命的化学 2020年第12期40卷 2151-2161页

作者：王志鹏张璇蒋振雄车子良马新雨 Division of Genetics Department of MedicineBrigham and Women's HospitalDepartment of Biological Chemistry and Molecular PharmacologyHarvard Medical SchoolBoston 02115USA Department of Chemistry Texas A&M UniversityCollege Station 77B40USA 清华大学化学系北京100084 清华大学生命学院北京100084 Department of Plant and Pathology and Microbiology Texas A&M UniversityCollege Station 77840USA Department of Biology Texas A&M UniversityCollege Station 77840USA

赖氨酸乙酰化是一类典型的组蛋白翻译后修饰,受到组蛋白乙酰转移酶(HAT)和组蛋白去乙酰化酶(HDAC)的共同精细调控。尽管HDAC一直是学科研究的前沿问题,但在国内科研综述或教学中缺乏相关内容的阐述。本文从HDAC的发现历史和去除乙酰化... 详细信息

赖氨酸乙酰化是一类典型的组蛋白翻译后修饰,受到组蛋白乙酰转移酶(HAT)和组蛋白去乙酰化酶(HDAC)的共同精细调控。尽管HDAC一直是学科研究的前沿问题,但在国内科研综述或教学中缺乏相关内容的阐述。本文从HDAC的发现历史和去除乙酰化的机制入手,在现有对HDAC分类的基础上,依次概述了4类HDAC的基本结构特征和生物功能。此外,本文还分析了针对不同HDAC的抑制剂及其在医学药学中的应用,旨在为相关的靶点研究及教学工作提供参考。

关键词：赖氨酸乙酰化去乙酰化酶组蛋白去乙酰化酶抑制剂